• The Journal of the Korean Society for Microbiology
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• v.19 no.1
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• pp.49-54
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• 1984

The authors isolated Vibrio parahaemolyticus from sea water, sea mud and various marine products in Busan shore area from 1981 to 1982, Among 100 isolated strains, 66 strains showed positive reaction in Kanagawa phenomenon. With the above 66 strains, the authors carred out test for detecting hemolysis activity of V.parahaemolyticus on human, rabbit, chicken, pig, goat, sheep and cow erythrocytes, in different media, such as modified Wagatsuma, nutrient, peptone and brain heart infusion agar plates media. The following results were obtained: 1. The media which can be used for Kanagawa phenomenon of V. parahoemolyticus were modified Wagatsuma, nutrient, peptone agar media, but not brain heart infusion agar medium. 2. The erythrocytes which showed positive Kanagawa phenomenon were those of human, rabbit, chicken and pig, but sheep, goat and cow erythrocytes showed no sensitivities.

• Korean Journal of Food Science and Technology
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• v.48 no.4
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• pp.323-328
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• 2016

In this study, specific and rapid enrichment and growth conditions for the most important, classic non-O157 enterohemorrhagic Escherichia coli (EHEC) serogroups were assessed. Three enrichment broth types, namely, EC medium with MUG broth, BRILA broth, and mTSB broth with novobiocin, were compared to identify the optimum enrichment broth for EHEC isolation. Four kinds of selective media, namely, ENDO agar, Chromocult agar, TBX agar, and CHROMagar$^{TM}$ STEC medium, were compared to identify the optimum one for non-O157 EHEC isolation. The results suggested that incubation in EC medium with MUG broth at $42^{\circ}C$ for 20 h is optimum for the enrichment of non-O157 EHEC. TBX agar was identified to have the highest specificity among the tested media. Consequently, a combination of complementary selective media according to serotype must be considered for comprehensive isolation of specific EHEC.

• Journal of Environmental Health Sciences
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• v.33 no.2 s.95
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• pp.145-149
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• 2007

The range of reduction rates of airborne bacteria concentration at 6 hrs, 12 hrs, 24 hrs and 48 hrs, which means a storage time until input of agar media into incubator after air sampling with an impactor. were 15-20%, 25-40%, 35-50% and 55-70%, respectively, compared to initial concentration. Types of agar media and storage thermal condition did not significantly affect a collection efficiency of impactor in terms of evaluating airborne bacteria level (p>0.05). To better improve the impactor's collection efficiency of airborne bacteria, based on the result of this study, it is recommended that the vicinity of $25^{\circ}C$ should be sustained until input of agar media into incubator after air sampling.

• Journal of Environmental Health Sciences
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• v.19 no.1
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• pp.30-36
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• 1993

A survey was conducted to measure concentration of airborne microbe in a hospital using RSC air sampler during October~November 1991.The result was as follows: 1) In an agar strip GK-A media for total counts of microbial particles. The highest count were 1384 CFU/m$^3$ in the main lobby, followed by 912 CFU/m$^3$, in the obstetric room, 688 CFU/m$^3$ in 1CU. By gram staining, the distribution for organisms in the air were shown 74.1% in gram possitive cocci followed by 16.8%, in gram possitive bacilli 6.7% in gram negative bacilli and 4.7% in yeast, but low organism was detected in recovery room with 194 CFU/m$^3$. 2) In agar strip S media for Staphylococci the count at the main lobby was detected in the recovery room with 92 CFU/m$^3$, Tests of coagulase were negative Staphylococci with 78%, and positive Staphylococci with 22%. The Staphylococci were highly resistance to penicillin, ampicillin and sensitive to amikacin, cefazolin, gentamycin and chloramphenicol. 3) In agar strip C media for coliform bacteria the colony counts at the main lobby was 139 CFU/m$^3$ and treatment room was 190 CFU/m$^3$, most frequently isolated microorganisms were non fermentative bacilli. 4) In agar strip HS media for yeast and molds. Most frequently colony counts 17~76 CFU/m$^3$, 0.5% lactophenol cotton blue stains were shown unidentified 77.2%, 8.1%, in Penicillium 8.1% in Aspergillus, and 3.8% in mucor.

• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.309-315
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• 1994

Selective agar media were constructed for the counting of bifidobacteria in dairy produ- cts containing bifidobacteria, lactobacilli and streptococci. This media containing antibiotics inhibited the growth of lactobacilli and streptococci at less than 10$^{5}$ cfu/ml, but had no influence on the recovery of bifidobacteria. In order to inhibit the growth of 10$^{5}$ cfu/ml of lactobacilli and streptoco- cci, the addition of 1.0~2.0 $\mu$g/ml of tetracycline in BL agar medium was needed. When 25 $\mu$g/ml of neomycin and paromomycin were mixed with 1.0 $\mu$g/ml of tetracycline in BL agar medium, it was able to inhibit the growth of 10$^{6}$ cfu/ml of lactobacilli and streptococci but had a little negative effect on the recovery of colonies of bifidobacteria. The results revealed that the BL agar medium containing 1.0 $\mu$g/ml of tetracycline was suitable to count the cell number of bifidobacteria selecti- vely in the presence of a 1 to 10$^{5}$-fold excess of L. casei, L. acidophilus and S. salivarius subsp.thermophilus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.7
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• pp.1025-1031
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• 2011

The objective of this study was to compare the performances of conventional microbiological media used in isolation of Shigella spp. from foods. Total of six selective media, including MacConkey agar (MAC), Salmonella Shigella agar (SSA), desoxycholate citrate agar (DCA), xylose lysine desoxycholate agar (XLD), hektoen enteric agar (HEA), and CHROMagar, were tested. MAC showed almost the same colony numbers as compared to tryptic soy agar (TSA) while DCA showed significantly lower colony numbers when cultivated Shigella spp. was counted in each medium. In a food recovery test with beef, pork and shrimp, S. sonnei recovered well on CHROMagar (p<0.05). With lettuce and cabbage, S. sonnei displayed significantly significant recovery (p<0.05) on SSA in comparison with other selective media. Heat-injured cells recovered well on MAC and SSA. In a specificity test using Enterobacteriaceae strains, HEA was identified as having the highest specificity among the tested media. However, Morganella spp. could not be differentiated from Shigella spp. on any of the tested selective media. Shigella spp. precluded the possibility of isolation from foods by a single 'best' selective medium. Consequently, a combination of complementary selective media or selection of appropriate media according to cell conditions must be considered for comprehensive isolation.

• Korean Journal of Microbiology
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• v.23 no.1
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• pp.43-48
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• 1985

Strict anaerobic procedures and anaerobic chamber were employed in order to improve the isolation of obligate anaerobes from oral pyogenic infections. Also different culture media were utilized to maximize bacterial recovery. Two blood media: nalidixic acid Tween blood agar (NATB) and plain blood agar (BA), two non-blood media: nalidixic acid Tween agar (NAT) and Gifu anaerobic medium (GAM) were used and compared for their isolation efficacy. Specimens from seven patients were collected with syringe. After collection, the needle was sealed with sterilixed silicone rubber and brought to labortory. The time spent from specimen collection to its processing in anaerobic chamber usually was 15 min. Identification of isolated bacterial strains was done with the API-20A system. Increase in isolation of anaerobic vacteria was achieved. Obligate anaerobic bacteria isolated were 3.3 strains per specimen. This figure falls within the range of 1.9-4.4 strains per specimen reported in other countries. With respect to the media utilized, blood media were superior to non-blood media. NATB medium was effective especially for the isolation of Gram-positive cocci. A total of 15 species of Gram-negative rods was isolated and 12 of them belonged to Bacteroides.

• Proceedings of the Korean Environmental Health Society Conference
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• 2002.04a
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• pp.3-5
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• 2002

A measurement of indoor microorganism using Reuter Centrifugal Air Sampler(RCS) was undertaken during October 1991-February 1999 and 6-Stage Cascade Air Sampler was undertaken during May 2001-June 2001 in Seoul. The results were as follows : 1. The average of total microorganism collected on the agar strip GK-A media were, in the order, subway station, hospital, underground shopping center, department store, book store, theater, sports facility, educational institution, office building in sites. 2. The average of staphylcocci collected on the agar strip GK-S media, in the order, were subway station, underground shopping center, hospital and department store, department store, theater, office building, sports facility and educational institution in sites. 3. The average of fungus collected on the agar strip GK-HS media, in the order, were Underground hospital, shopping center, theater, subway station, department store, book store, sports facility, educational institution, and office building in sites

• Journal of the Korean Home Economics Association
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• v.19 no.4
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• pp.9-15
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• 1981

damages of cotton cloth and characteristics of fabroid degradation were studied by Chaetomium globosum and Aspergillus niger which presupposed as powerful erosive fungi to cellulose fiber by means of tensile strength. The results obtained are as follows: 1. the growth(rate) of fungi in malt extract agar was superior to potato agar for two weeks. 2. Chaetomium globosum showed mostly severe damage t the cotton cloth in malt extract agar media at pH 4.5. 3. Tensile strength was reduced with time by Aspergillus niger-coenzyme and Chaetomium globosum-coenzyme reaction. In comparison with Chaetomium globosum and Aspergillus niger, the former weaken tensile strength about 15.8% and the latter enfeebled 10.0% after 124 hours. 4. after 30 days the breeding of fungi in pH 4.5 malt extract agar media, critical damage of cotton cloth was observe, I. e., 92.4% damage by chaetomium globosum and 74.9% lose by aspergillus nige respectively.

• The Korean Journal of Mycology
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• v.10 no.1
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• pp.7-13
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• 1982

Habit characteristics of imperfect and perfect stage of D. bryoniae encountered on naturally infected seeds of cucumber and pumpkin were studied by the blotter method and compared with those grown on Difco potato dextrose agar (PDA), V-8 juice agar and water agar leaf medium (WALM). Most of the pycnidiospores obtained from each isolate of this fungus grown on PDA were non-septate and microtype. Non-septate pycnidiospores were predominanted in all isolates, but a macrotype of the non-septate and a number of uniseptate pycnidiospores were produced on V-8 juice agar and water agar leaf medium. On seed the pycnidiospores were mostly non -septate, but rarely uniseptate ones were also found. On radicle of cucumber seed, the pycnidiospores were non-septate and uniseptate but small percentage biseptate with somewhat constricted at septa. Pycnidiospores produced on V-8 juice agar and water agar leaf medium were similar to those produced on seeds. In the present investigation the perithecia were mostly globose to subglobose with apical papillate ostiole and whitish spore masses formed on the ostiole of perithecia, either on naturally infected seed or on culture media. The mature perithecia were dark brown to black. They were partially embedded or erumpent on seed coat and culture media. The perithecia varied in size within a much narrower range than the pycnidia. But perithecial formation of this fungus on PDA, V-8 juice agar, WALM and seed varied considerably depending upon isolate and culture media.