Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selective Media Containing Antibiotics for Counting Bifidobacteria

항생제를 이용한 Bifidobacteria의 선택배지

  • 이정준 (한국 야쿠르트 유업(주) 연구소) ;
  • 신명수 (한국 야쿠르트 유업(주) 연구소) ;
  • 나석환 (한국 야쿠르트 유업(주) 연구소) ;
  • 배형석 (한국 야쿠르트 유업(주) 연구소) ;
  • 백영진 (한국 야쿠르트 유업(주) 연구소)
  • Published : 1994.06.01
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Abstract

Selective agar media were constructed for the counting of bifidobacteria in dairy produ- cts containing bifidobacteria, lactobacilli and streptococci. This media containing antibiotics inhibited the growth of lactobacilli and streptococci at less than 10$^{5}$ cfu/ml, but had no influence on the recovery of bifidobacteria. In order to inhibit the growth of 10$^{5}$ cfu/ml of lactobacilli and streptoco- cci, the addition of 1.0~2.0 $\mu$g/ml of tetracycline in BL agar medium was needed. When 25 $\mu$g/ml of neomycin and paromomycin were mixed with 1.0 $\mu$g/ml of tetracycline in BL agar medium, it was able to inhibit the growth of 10$^{6}$ cfu/ml of lactobacilli and streptococci but had a little negative effect on the recovery of colonies of bifidobacteria. The results revealed that the BL agar medium containing 1.0 $\mu$g/ml of tetracycline was suitable to count the cell number of bifidobacteria selecti- vely in the presence of a 1 to 10$^{5}$-fold excess of L. casei, L. acidophilus and S. salivarius subsp.thermophilus.

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References

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