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http://dx.doi.org/10.9721/KJFST.2016.48.4.323

Evaluation of enrichment broth and selective media for the detection of non-O157 enterohemorrhagic Escherichia coli  

Lee, Da Yeon (Food Analysis Center, Korea Food Research Institute)
Kim, Hee-eon (Food Analysis Center, Korea Food Research Institute)
Seo, Dong Won (Food Analysis Center, Korea Food Research Institute)
Cho, Yong Sun (Food Analysis Center, Korea Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.48, no.4, 2016 , pp. 323-328 More about this Journal
Abstract
In this study, specific and rapid enrichment and growth conditions for the most important, classic non-O157 enterohemorrhagic Escherichia coli (EHEC) serogroups were assessed. Three enrichment broth types, namely, EC medium with MUG broth, BRILA broth, and mTSB broth with novobiocin, were compared to identify the optimum enrichment broth for EHEC isolation. Four kinds of selective media, namely, ENDO agar, Chromocult agar, TBX agar, and CHROMagar$^{TM}$ STEC medium, were compared to identify the optimum one for non-O157 EHEC isolation. The results suggested that incubation in EC medium with MUG broth at $42^{\circ}C$ for 20 h is optimum for the enrichment of non-O157 EHEC. TBX agar was identified to have the highest specificity among the tested media. Consequently, a combination of complementary selective media according to serotype must be considered for comprehensive isolation of specific EHEC.
Keywords
enterohemorrhagic Escherichia coli; enrichment broth; selective media; evaluation;
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