• Title/Summary/Keyword: Agar

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Effects of Light and Media on Pycnidial Formation of Didymella bryoniae (Auersw.) Rehm (병자곡 및 자양곡형성에 미치는 광선 및 배지의 영향)

  • Lee D. H.
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.211-215
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    • 1977
  • The Sporulation of Didymella bryomiae were observed under diurnal cycles of light/darkness of near ultraviolet light (NUV) and artificial daylight (ADL) and continous darkness in eight isolates growing on PDA and V-8 juice agar. Light stimulated pycindial and perithecial formation of this fungus on potato dextrose agar and V-8 juice agar. Sprulation was poor in darkness, but some isolates were able to produce pycnidia and perithecia in the absence of light. Perithecial formation was much better under artificial daylight (ADL) on V-8 juice agar than those grown under near ultraviolet light (NUV). In general, cultures grown on V-8 juice agar sporulated better than cultures grown on PDA under three setsof light condition. Most of the pycnidiospores obtained from each isolates of this fungus grown on PDA were non-septate and microtype, but macrotype of non-septate and uniseptate pycnidiospores were produced on V-8 juice agar. Pycnidiospore produced on V-8 juice agar were similar to those produced on the radicle of naturally infected seeds. The appearance of perithecia were quite distinctive from pycnidia. The mature perithecia were darker than pycnidia and whitish spore masses formed on the ostiole of perithecia.

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Isolation and Identification of Marine Bacterium Cytophaga sp. AYK301 and Optimal Culture Conditions for the Production of Agarase (해양세균 Cytophaga sp. AYK301의 분리.동정 및 한천분해효소 생산을 위한 최적배양조건)

  • Lee, Won-Gyeong;Kim, Bong-Jo;Ha, Sun-Deuk;Gong, Jae-Yeol
    • KSBB Journal
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    • v.14 no.5
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    • pp.572-577
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    • 1999
  • A marine bacterium with highly effective agar degrading activity was ioslated from the southern sea of Korea (Chonnam, YoChon) and identified as Cytophaga sp. and named as Cytophaga sp. AYK301. This strain produced an extracellular agarase which had a high activity with agar. The optimum culture conditions for the production of agarase have been determined. For the increase of agarase productivity, 0.2% agar, 0.3% beef extract, and 0.05% NH$_4$NO$_3$ were used as carbon, organic and inorganic nitrogen source, respectively. The optimal initial pH, NaCl, culture time and temperature for the agar degrading activity were 7.5, 7.0%, 36 hr and $25^{\circ}C$, respectively. In the optimal conditions, the agarase production was increased up to more than 4.0 folds as compared to that by the basal medium.

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Distribution of Microorganisms Isolated from Cellular Phones

  • Kim, Su-Jung;Kim, Ga-Hyun;Shin, Eun-Kyung;Bae, Ji-Hyun;Jeon, Yu-Ri
    • Biomedical Science Letters
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    • v.14 no.4
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    • pp.257-261
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    • 2008
  • Cellular phones are the most used electronic device everyday in modern life and are always in contact with our hands, Although many studies have revealed microorganisms living on our hands, there are only a few reports on the research about products or places which are in contact with our hands. Therefore, the purpose of this study is to verify microorganisms living in cellular phones. Microorganisms were scraped from cellular phones of students and professors from the clinical laboratory science department in Daegu Health College, and cultured at Brain Heart Infusion agar and MacConkey agar following API kit to identify them. The average colony number was $1.5{\times}10^2$ on BHI agar and $40{\times}10$ on MacConkey agar. There was no difference according to gender. In Gram stain result, Gram(+) Cocci showed the highest frequency. Also in BHI agar plates, Micrococcus spp and Acinetobacter baumannii identified with high frequency. Moreover, S. aureus, which is very well known as strong food poisoning bacteria, was isolated. Klebsiella pneumonia ssp pneumonia was isolated with the highest frequency from the MacConkey agar or S-S agar plate. From these results show, there are as many different microorganisms from cellular phones as from our hands. This is the first report isolating strong food poisoning bacteria in cellular phones. Since infection in hospitals have been an important issue to be aware of, it is equally necessary to investigate cell phones and products which hospital workers touch with their hands.

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Experimental Investigations on the Characteristics of Explosion Mitigations by Different Concentrations of Agar Gel Barriers (Agar Gel Barrier의 농도변화에 따른 폭발완화 특성에 관한 실험적 연구)

  • Park, Dal-Jae;Kim, Nam-Il
    • Journal of the Korean Institute of Gas
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    • v.15 no.5
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    • pp.13-18
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    • 2011
  • Experimental studies were carried out to investigate the characteristics of explosion mitigations by varying concentrations of agar gel barriers in an explosion chamber, 1400 mm in length, with a square cross-section of $100{\times}100mm^2$. Another extension chamber, $100{\times}100{\times}300mm^3$, was made to hold a gel barrier. Four different gel concentrations were used in the measurements: 2, 3, 4, 5 %(by weight of gel). Displacement of the gel barrier was measured using a high speed camera, and pressure development was measured using pressure transducers and a data acquisition system. It was found that as the concentrations of the gel barriers increased, the gel rupture time and the time taken to reach the maximum pressure increased. It was also found that the increment of gel concentrations increased the reduction percentage in the maximum pressure between before and after gel barrier.

Cultural Characteristics of Chromogenic and Teleomorphic Strains of Collectotricum gloeosporioides Isolated from Apple and Red pepper (사과와 고추에서 분리한 Colletotrichum gloeosporioides의 색소형성형 및 유성세대형 계통의 배양적 특징)

  • Lee, Du-Hyung
    • The Korean Journal of Mycology
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    • v.25 no.4 s.83
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    • pp.340-347
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    • 1997
  • Conidia and cultural characteristics of isolates of chromogenic and teleomorphic strains of Colletotrichum gloeosporioides from apple and red pepper were compared. The mycelial growth of teleomorphic strains was faster than that of chromogenic strains in potato dextrose agar and V-8 agar. The chromogenic isolates from apple and red pepper developed. white gray to gray green mycelial rings interspersed with salmon to apricot colored conidial masses in colonies on potato dextrose agar and V-8 agar and none formed on ascigerous stage in cultures. The chromogenic isolates from red pepper produced conidia, most with one apex attenuated on apple and potato dextrose agar whereas fusiform and smaller conidia were produced in V-8 agar and water agar leaf medium. The chromogenic isolates from apple produced fusiform conidia in the media tested. The teleomorphic isolates from apple and red pepper produced cylindrical conidia, most with both apices rounded, developed white gray to dark olive green in a zonate pattern with small dark spots throughout colonies and formed the ascigerous stage in cultures.

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Sutdies on Quality Characteristics of Jeju Mandarin Orange Jelly for the Aged (고령자용 감귤젤리의 품질 특성 연구)

  • Lee, Ji-Eun;Choi, Eun-Jung;Oh, Myung-Suk
    • Journal of the Korean Society of Food Culture
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    • v.22 no.4
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    • pp.475-481
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    • 2007
  • This study was carried out to determine the quality characteristics of Jeju mandarin orange jelly with various gelling agent such as agar, ${\kappa}-carrageenan$ and gellan gum for the aged. The concentration of agar was 0.4-0.6% and that of ${\kappa}-carrageenan$ and gellan gum was 0.2-0.4%. The color value, gelling temperature, melting temperature, break down rate, textural properties and sensory acceptance test of Jeju mandarin orange jelly with various gelling agent were measured. Average age of the subjects for acceptance test was 78.23. Redness and yellowness of Jeju mandarin orange jelly with agar was lower than that with ${\kappa}-carrageenan$. The gelling and melting temperature of Jeju mandarin orange jelly with agar was lowest among the jellies. Break down rate of Jeju mandarin orange jelly with agar was highest among the jellies. Above results showed that the stability of Jeju mandarin orange jelly with agar was inferior than that with ${\kappa}-carrageenan$ and gellan gum. Hardness, adhesiveness and springiness of Jeju mandarin orange jelly with agar was lowest among the jellies and sensory acceptance of Jeju mandarin orange jelly with gellan gum was highest among them. Thus, gellan gum was appropriate gelling agent for the Jeju mandarin orange jelly with regard to the acceptability and the depression of sour taste in Jeju mandarin orange jelly could improve the acceptability for the aged.

Functional Properties of Hydrolysates Prepared from Agar Treated with Organic Acids (한천 유기산 가수분해물의 생리활성)

  • Joo, Dong-Sik;Lee, Chang-Ho;Cho, Soon-Yeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.12
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    • pp.1548-1553
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    • 2008
  • This study was concerning various physiological activities of agar hydrolysates. All agar hydrolysates showed strong antimicrobial activity against Bacillus subtilis and Bacillus cereus. Also, the agar hydrolysates prepared at the temperature of $110^{\circ}C$ or $120^{\circ}C$ showed antimicrobial activity against St. aureus and E. coli. Among the agar hydrolysates, several hydrolysates treated with citrate or malate at $110^{\circ}C$ or $120^{\circ}C$ conditions showed tyrosinase activity inhibition, and their inhibition rates of tyrosinase activity were about 80%. Some tested samples treated with 0.5% organic acid at $100^{\circ}C$ or $110^{\circ}C$ inhibited the growth of cancer cell. Two agar hydrolysates prepared with 0.5% citrate and lactate at $110^{\circ}C$ for $180^{\circ}C$ min had relatively high cancer cell growth inhibition among the tested samples. The agar hydrolysates treated with citrate and lactate at $110^{\circ}C$ for 180 min obtained the main peaks of six and seven from Sephadex G-15 column chromatography. Among the main peaks, the cancer cell growth inhibition of C-3 and L-3 fractions were higher than that of other fractions.

Evaluation of enrichment broth and selective media for the detection of non-O157 enterohemorrhagic Escherichia coli (Non-O157 장출혈성대장균 검출을 위한 증균배지 및 선택배지 성능 평가)

  • Lee, Da Yeon;Kim, Hee-eon;Seo, Dong Won;Cho, Yong Sun
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.323-328
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    • 2016
  • In this study, specific and rapid enrichment and growth conditions for the most important, classic non-O157 enterohemorrhagic Escherichia coli (EHEC) serogroups were assessed. Three enrichment broth types, namely, EC medium with MUG broth, BRILA broth, and mTSB broth with novobiocin, were compared to identify the optimum enrichment broth for EHEC isolation. Four kinds of selective media, namely, ENDO agar, Chromocult agar, TBX agar, and CHROMagar$^{TM}$ STEC medium, were compared to identify the optimum one for non-O157 EHEC isolation. The results suggested that incubation in EC medium with MUG broth at $42^{\circ}C$ for 20 h is optimum for the enrichment of non-O157 EHEC. TBX agar was identified to have the highest specificity among the tested media. Consequently, a combination of complementary selective media according to serotype must be considered for comprehensive isolation of specific EHEC.

Comparison of the E-Test with Agar Dilution Susceptibility Test by Using Bacteroides fragilis (Bacteroides fragilis의 E-test와 한천 평판 희석법에 의한 항균제 감수성상의 비교)

  • Kim, Hee-Sun;Kim, Sung-Kwang;Cha, Hwa-Sun
    • Journal of Yeungnam Medical Science
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    • v.10 no.1
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    • pp.135-143
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    • 1993
  • The susceptibilities of 45 clinical isolates of bacteroides frogilis to cefaclor, ciproflxacin and imipenem were determined by new method, E-test (AB Bidisk, Solna, Sweden) and were compared with those from conventional agar dilution method by using brain heart infusion, Mueller-Hinton and Wilkins Chalgren agar plates. And the susceptibility of 60 clinical isolates of Bacteroides fragilis group (B. fragilis 45 strains, B. distasonis 6 strains, B. ovatus 5 strains, B. thetaiotaomicron 4 strains) to 5 quinolones (ciprofloxacin, enoxacin, norfloxacin, ofloxacin, pefloxacin) were determined by in vitro agar dilution method. Compared with agar dilution MICs for B. fragilis 45 strains, 90.3% of E-test MICs were within ${\pm}$1 dilution of the agar dilutions, and 98.4% were within 2 dilutions. And there were little effect of different medium bases to determine MICs except Mueller-Hinton agar. On Mueller-Hinton agar, B. fragilis showed have or no growth activity. In vitro susceptibility of B. fragjlis group to quinolones, most of the test strains showed resistant patterns to quinolones except ofloxacin and there was little difference of susceptibility patterns between species of B. fragilis group.

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Comparison of Temperature Distribution in Agar Phantom and Gel Bolus Phantom by Radiofrequency Hyperthermia

  • Jung, Dong Kyung;Kim, Sung Kyu;Lee, Joon Ha;Youn, Sang Mo;Kim, Hyung Dong;Oh, Se An;Park, Jae Won;Yea, Ji Won
    • Progress in Medical Physics
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    • v.27 no.4
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    • pp.224-231
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    • 2016
  • The usefulness of Gel Bolus phantom was investigated by comparing the temperature distribution characteristic of the agar phantom produced to investigate the dose distribution characteristic of radiofrequency hyperthermia device with that of the Gel Bolus phantom under conditions similar to those of an agar phantom that can continuously carry out temperature measurement. The temperatures of the agar phantom and the Gel Bolus phantom were raised to $36.5{\pm}3^{\circ}C$ and a temperature sensing was inserted at depths of 5, 10, and 15 cm from the phantom central axis. The temperature increase rate and the coefficient of determination were analyzed while applying output powers of 100 W and 150 W, respectively, at intervals of 1 min for 60 min under conditions where the indoor temperature was in the range $24.5{\sim}27.5^{\circ}C$, humidity was 35~40%, internal cooling temperature of the electrode was $20^{\circ}C$, size of the upper electrode was 250 mm, and the size of the lower electrode was 250 mm. The coefficients of determination of 150 W output power at the depth point of 5 cm from the central axis of the phantom were analyzed to be 0.9946 and 0.9926 in the agar and Gel Bolus phantoms, respectively; moreover, the temperature change equation of the agar and Gel Bolus phantoms with time can be expressed as follows in the state the phantom temperature is raised to $36^{\circ}C:Y(G)$ is equation of Gel Bolus phantoms (in 5 cm depth) applying output power of 150 W. Y(G)=0.157X+36. It can be seen that if the temperature is measured in this case, the Gel Bolus phantom value can be converted to the measured value of the agar phantom. As a result of comparing the temperature distribution characteristics of the agar phantom of a human-body-equivalent material with those of the Gel Bolus phantom that can be continuously used, the usefulness of Gel Bolus phantom was exhibited.