• 한국가축번식학회지
• /
• 제16권1호
• /
• pp.21-38
• /
• 1992

The present study was carried out to investigate morphologic changes in the corpus luteum of the pregnant rat by electron microscope after administration of prostaglandin F2$\alpha$(PGF2$\alpha$). Pregnant rates were treated with PGF2$\alpha$(1,500$\mu\textrm{g}$/rat) and their corpura lutea were observed morphologically. The results obtained in this study were summarized as follows ; 1. The weight of the ovaries and corpura lutea were decreased slightly at 8~24 hours after PGF2$\alpha$ administratin but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12~48 hours and 18~24 hours after PGF2$\alpha$ tretment but there were no signifciant differences between control and treatment. 3. The weight of uterus and the unmber of embryo decreased slightly at 96 hours and at 18~96 hours after PGF2$\alpha$ administration but no significant differences were obtained. 4. In the electron microscopic observatons, lipid droplets which are electron dense and appear in the cytoplasm moderately increased in number after PGF2$\alpha$ treatment. The lipid droplets were surrounded by mitochodria and appeared in the autophagic vacuoles. 5. Moderated and high electron dense mitochondria which are round or elongated in shape showed pleomorphism from 3 hours after PGF2$\alpha$ treatment. Destruction of tubular of vesicular cristae was observed at 6 hours after the treatment. Dense body and myelin figures in matrix of mitochondria were also appeared. 6. Well-developed smooth endoplasmic reticulum(sER) showed tubular or vesicular cisternae. A number of whorl membranes containing ribosomes, mitochondria and lipid droplets were observed at 1.5 hour after treatment. sER was abundant in luteal cells at 12 hours were treatment. 7. Well-developed Golgi pparatus appeared obviously 6 hours and more prominently at 12 hours. Those Golgi vesicles were remarkably dilated. 8. Generally, a few rough endoplasmic reticulum (rER) were appeared after treatment and cisternae showed slight dilatation. No differences among the treatments were observed. However, slight dilation of cisternae was observed at 1.5 hours after treatment. 9. Ribosomes composed of free and polyribosomes were abundant before treatment but polyribosomes were appeared at 12 to 24 hours after treatment. 10. Intercellular space were slightly extended at 3 hours and markedly extended at 12 hours. Numerous microvillous protrusions were observed at these times. Membranous multivesicular structures and autophagic vacuoles were also appeared in the intercellular space. 11. At 3 hours after the treatment, autophagic vacuoles appeared in the cytoplasm of the cell. They increased in number with time and were observed to transfer to the intercellular space. Lysosomal dense body appeared in the cytoplasm and the inclusion body was also observed in nucleus at 12 to 24 hours after treatment.

• 한국가금학회지
• /
• 제16권1호
• /
• pp.23-28
• /
• 1989

난백단백질의 농도와 당류의 첨가 수준이 난백의 열감수성에 미치는 영향을 검토하기 위하며 가열처리.($60^{\circ}C$, 5분간) 전후 난백의 기능성을 측정하였으며 그 결과는 다음과 같다. 난백단백질의 농도 8.3%까지는 가열처리 전후 난백의 탁도가 감소하였으나 희석됨에 따라 증가하다가 3.32% 이하에서는 다시 감소하였다. 가열처리 전후 난백의 기포력은 단백질 농도 8.3%에서 가장 높았으며 희석됨에 따라 점차 감소하였고 기포안정성은 단백질 농도가 희석됨에 따라 점차 저하하였다. 난백에 sucrose를 첨가할 경우 탁도의 변화가 없었으나 가열처리의 경우에는 sucrose의 첨가량에 따라 점차 감소하였다. 기포력은 sucrose 5%이상 첨가 시 감소하였으며 가열처리의 경우에는 sucrose 2.5%이상 첨가 시 점차 감소하였다. 기포안정성은 sucrose를 5%이상 진가할 경우 점차 증가하였으나 가열처리한 경우에는 sucrose의 첨가량에 따라 증가하였다. Glucose의 첨가량에 따라서는 탁도가 높아졌으며 가열처리 시에는 변화가 없었다. 기포력은 Glucose의 첨가량에 따라 점차 감소하였으며 가열처리의 경우에는 5%이상 첨가 시 다소 감소하였다. 기포안정성은 가열처리 전후 glucose의 첨가량에 따라 증가되었으며 sucrose의 첨가시 보다 크게 증가되었다.

• 한국가축번식학회지
• /
• 제4권1호
• /
• pp.35-45
• /
• 1980

The purpose of this experiment was to investigate the effects of castration and administration of testosterone propionate(TP) on the development of the thyroid, adrenal glands and the testis in immature male rats, 25 days immatured, weighing 64.1${\pm}$2g, were divided into two groups of control and castrated, each sub-divided into 30 rats again, treated and untreated with TP respectivity. Each rat was given 20$m\ell$ of TP subcutaneously at two weeks interval. Six rats among each group were randomly sacrificed at 7, 21, 35, 49, and 63 days after treatment, of which their thyroid, adrenal glands and testis were collected for cytometric observation. The results obtained were as follows. 1. The size of the follicle of thyroid glands had a tendency to increase proportionally to the treatment period in every group. However, in castration group, the follicular size of the untreated with TP were significantly increased from 49 days (p<0.05) after treatment than that of the treated with TP, while in control group, the treated with TP were not increased during the treatment period. Regarding the height of the follicular epithelial cell in thyroid gland, the treated with TP had a tendency to increase than the untreated with TP in both castration and control group. 2. Regarding the size of the follicle of thyroid gland in relation with the increment period, the untreated with TP in control group were slightly increased from 49 days after treatment, but the treated with TP were not changed significantly. Castration group had a tendency to increase significantly than the control group, especially the untreated with TP in castration group were significantly increased. 3. As for the change of the relative height of thyroidal follicular epithelial cell in relation with the increment of treatment period, the untreated, A and C group, in both castration and control group were increased at 35 days 63 days after treatment while the treated, B and D group had tendency to increase from 21 days after treatment. 4. Regarding the thickness fo adrenal cortex, the castration group had a tendency to increase than the control group until 21 days after treatment. But, at 35 days, the change of the thickness was reversed; Mean while at 49 days and 63 days, especially C group in castration were significantly increased than any other groups although there were no significant differences among the every group during the whole treatment period. Regarding the thickness of adrenal cortex in relation with the increment of treatment period, A, B and C group had a tendency to increase until 21 days after treatment. After that period, there was no significant increment in all groups. Especially, in D group, there were no significant changes from 7 days to 63 days after treatment. 5. As for the tickness of adrenal medulla, there were no significant changes in every group of castration and TP treatment, except that the castration group had a tendency to increase continually than the control throughout the whole treament period. 6. In terms of the number, diameter and thickness of seminiferous tubule in testis of control group, the treated group with TP were distinctly reduced than those of the untreated group from 49 days after treatment respectively.

• 한국가축번식학회지
• /
• 제3권1호
• /
• pp.48-55
• /
• 1979

This experiment was conducted to investigate the effects of ovariectomy and estradiol benzoate administration on body growth and development of endocrine glands in female rats. One hundred and twenty female rats, 30 days old weighing 72.0${\pm}$3.0g, were divided into control and ovariectomized groups and divided again into two groups, treated and untreated with estradiol benzoate, respectively. Each group was composed of 30 rats. Hormone was injected every two week interval. The body weight was measured weekly from the beginning of treatment to 63 days after treatment and 6 rats per group were sacrificed at 7, 21, 35, 49 and 63 days after treatment for weight change of endocrine gland. The results obtained were as follows: 1. the changes in the body weight during the experimental period were not signifilcantly different between two groups, although the body weight of ovariectomized groups were heavier than that of control groups and the body weight of treated group with estradiol benzoate was heavier than that of untreated group. 2. The body lengths in all groups were increased proportionally to body weight until 63 days after treatment and there were not significantly different among the experimental groups. 3. The weight of hypophysis treated with estradial benzoate in ovariectomized groups was continuously increased in the whole period and was highly significant at 49 and 63 days after treatment as compared with those of untreated in ovariectomized group. 4. Adrenal gland weights of the control and ovariectomized group treated with estradiol benzoate were distinctly light at 21 days after treatment, but there were not significantly different among all groups at 35 days. Adrenal gland weight of ovariectomized groups was smaller than control groups at 49 days and untreated group in ovariectomized groups was considerably smaller than the other 3 groups at 63 days after treatment. 5. In control rats, the ovary weight treated group with estradiol benzoate was larger than the untreated group at 35 days after treatment, but was smaller than the untreated group after 49 days. 6. The weight of reproductive tract in ovariectomized groups were markedly smaller than control groups till 35 days after treatment. The growth of reproductive tract untreated in ovariectomized groups was almost completely depressed.

• Journal of Acupuncture Research
• /
• 제19권6호
• /
• pp.12-23
• /
• 2002

Objective : Assuming That the Characteristic of Meridian System Has been Similar to This of Electric Potentials in Human Body And that Measurements of Electric Potential at Well(井穴) and Sea(合穴) Points in Branches of the Twelve Meridians(WSBTM) Will Be Representative of Measurements of the Twelve Meridians, to Measure the Electric Potentials of 21 Patients with Shoulder Lesions, And Aching of Shoulder and Arm(肩臂痛)(ASA), to Be Differences in Electric Potentials of Patients Between Before And After Acupuncture Treatment, And Then to Find out the Characteristic of Meridian System in Patients with Shoulder Lesions. Methods : Electric Potentials of Well And Sea Points in the Meridians in Twenty one Patients with ASA Diagnosed by Both x-ray And Self View on Pain at Shoulder And Arm were Repeatedly Measured by Physiograph(PowerLab) at Before And after Acupuncture Treatment, Respectively. Measurements of Those Electrical Potentials Were Analyzed by Factor Analysis. Results : The Electric Potentials of WSBTM At the Left Side Before Acupuncture Treatment Were Divided Into Five Factors, But Those After Acupuncture Treatment Were Done Into Four Factors. On The Other Hand Those At the Right Side Before Acupuncture Treatment Were Divided Into Four Factors, The Number of Factors After Acupuncture Treatment Was Five. Conclusions : In Conclusions, The Number of Factors Before Acupuncture Treatment Are Different from Those After Acupuncture Treatment. In the Results The Effect of Acupuncture Treatment May be Reflected in Measurements of Electric Potential at WSBTM.

• The Journal of Korean Physical Therapy
• /
• 제14권3호
• /
• pp.60-73
• /
• 2002

Spasticity is the most troublesome problems in the management in cerebral palsy. The purpose of this study was to observe the effect of FES to spasticity. 8 cerebral palsy children were selected for this study. Assessment was carried out before treatment for obtain baseline measurement of spasticity and reassessment were carried out at after 10th., 20th. treatment sessions and 24hours after treatment. The results of this study were as following that MAS scores were significantly reduced after 10th and 20th after treatment compared with pre-treatment. MAS scores were significantly reduced after 20th compared with pre-treatment. These results indicated that FES appears to reduce significantly MAS scores and maintained 24hours after treatment compared with pre-treatment.

• Journal of Acupuncture Research
• /
• 제21권4호
• /
• pp.53-64
• /
• 2004

Objective: This study was to evaluate the influence of stress on Peripheral Facial Paralysis during the complex traditional korean medical treatment. Methods: We investigated 41 cases of patients with Peripheral Facial Paralysis who were given the complex traditional korean medical treatment. one group had stress as main factor, the other group didn't it. we evaluated the treatment effect of each group by using Gross Grading System of House-Brackmann, Yanagihara's Unweighed Grading System. Results : 1. In age, sex, lesion, duration of disease, frequency of treatment, duration of treatment, we found that two groups have no significant differences. 2. Pain back of the ear showed the highest frequency in symptoms at onset. 3. Boyangwhanotang showed the highest frequency in oriental herb medicine treatment. 4. As a result of evaluation by using Gross Grading System of House-Brackmann, Yanagihara's Unweighed Grading System, treatment score after 2weeks was marked more higher than score before treatment and treatment score after final treatment was more higher than treatment score after 2weeks on each groups. 5. After final treatment, Non-stress group had signficant result on Gross Grading System of House-Brackmann, Yanagihara's Unweighed Grading System compared with stress group. Conclusion : These results suggested that patient with Non-stress group should be get better than stress group.

• 열처리공학회지
• /
• 제37권3호
• /
• pp.128-137
• /
• 2024

In this study, the carburizing heat treatment process used in aircraft gear manufacturing was compared with the general carburizing heat treatment process using AISI 9310 steel. The process of carburizing followed by slow cooling, and then quenching after austenitizing(Process A) showed less compressive residual stress and less retained austenite in the surface layer compared to the process of quenching directly after carburizing(Process B). In prpcess B, there was a large amount of retained austenite when quenched immediately after carburization, and when treated with subzero, martensite rapidly increased and the compressive residual stress increased significantly, but at the same time, there is a risk of cracking due to severe expansion in volume. Therefore, in the case of aviation parts, it is believed that a step-by-step heat treatment cycle was adopted to ensure stability against heat treatment cracks. As a result of the final tempering after sub-zero treatment, the A process specimen showed a deeper effective case depth and HV700 depth and a higher hardness value above HV700 than the B process specimen.

• 동의생리병리학회지
• /
• 제22권6호
• /
• pp.1626-1632
• /
• 2008

Among patients who are receiving treatments at an oriental medical hospital for various symptoms and diseases, patients whose main disease is accompanied by metabolic syndrome with abnormal liver function. This research was performed in order to observe the progression of changes in the liver function and serum lipid profile after the oriental medical treatments to patients who have been receiving oriental medical treatment for various other diseases and have been diagnosed as having metabolic syndrome at their first visit to the hospital based on NCEP ATP III diagnosis criteria and WHO Asia Pacific region criteria. Total number of subject patients were 39cases(mean age:55.58${\pm}$2.09 years) which had 20 male and 19 female. For the references for hepatic enzyme levels and blood lipid profile were measured in before treatment and four times after treatments(every 2.31${\pm}$06.17 weeks). Serum AST was 48.86${\pm}$7.46 IU/L before oriental medical treatment. After the treatment, 40.63${\pm}$4.69, 43.12${\pm}$5.46, 37.82${\pm}$4.52 IU/L were measured where although the level decreased to the normal level compared to pre-treatment, the value was not significant statistically(P>0.05). ALT was 66.26${\pm}$11.01 IU/L before oriental medical treatment. After the treatment 62.10${\pm}$8.20, 61.10${\pm}$8.76, 43.79${\pm}$5.68 were measured where although the level decreased, abnormally high level was maintained. The last result was significant statistically(P<0.05) compared to pre-treatment. ALP was 193.06${\pm}$14.20 IU/L before oriental medical treatment. After the treatment, 176.80${\pm}$6.48, 177.46${\pm}$11.81, 162.41${\pm}$9.06 where although compared to pre-treatment the last result was significant statistically(P<0.05), the change was within the normal range. ${\gamma}$-GGT was 87.83${\pm}$12.59 IU/L before oriental medical treatment. After the treatment, progressively near normal level was achieved with 118.73${\pm}$46.45, 85.03${\pm}$17.12, 70.64${\pm}$10.93 and the last result was statistically significant compared to pre-treatment (P<0.05). Blood triglyceride was 217.63${\pm}$32.18 mg/dL before oriental medical treatment. After treatment 215.09${\pm}$22.18, 189.93${\pm}$22.44, 191.22${\pm}$18.51 where abnormal values continued even after treatment although results was not statistically significant compared to pre-treatment(P>0.05). Total-cholesterol was 197.28${\pm}$9.24 mg/dL before oriental medical treatment, after treatment 201.55${\pm}$11.13, 186.87${\pm}$8.77 and 186.68${\pm}$7.61 were measured that results were not statistically significant compared to pre-treatment(P>0.05). HDL-cholesterol was 41.88${\pm}$2.38 mg/dL before oriental medical treatment, after treatment 48.75${\pm}$4.22, 44.10${\pm}$1.91, 48.00${\pm}$2.06 the results were not statistically significant compared to pre-treatment(P>0.05). LDL-cholesterol was 111.66${\pm}$13.08 mg/dL before oriental medical treatment, after treatment 109.94${\pm}$10.18, 101.79${\pm}$8.63, 104.00${\pm}$6.98 the results were not statistically significant compared to pre-treatment(P>0.05). With such results, even if common oriental medical treatments were given to metabolic syndrome patients with abnormal liver function, the liver function was confirmed not to be aggravated, and the concentration of lipids in the blood was confirmed not to be affected in most patients.

• 한국가축번식학회지
• /
• 제7권1호
• /
• pp.41-51
• /
• 1983

The purpose of this experiment was to investigate the effects of adrenalectomy and PMSG treatment on reproductive organs and serum steroid hormone level in immature female rats. The animals used in this experiment were 25 days old female rats weighing a, pp.oximately 70g. They were randomly divided into two groups of intact rat group (Int-) and adrenalectomized rat group (Adx-) and each group were subdivided into two groups of Non-PMSG (-Cont) and PMSG treated (-PMSG) group. The rat of PMSG-treated group (-PMSG) was administered subcutaneously with 25 IU PMSG on first day (9 a.m.) after adrenalectomy. The adrenalectomized rat groups were su, pp.ied with saline solution through the experiment period. The rate of ovulation and vaginal opening and reproductive organ weights were observed at 8, 32, 56, 80 and 104 hours after PMSG treatment. At the same time, the serum level of estradiol-17${\beta}$ and progesterone were measured by the radioimmunoassay. The results obtained were as follows: 1. Ovulation was shown at 56 hours after treatment in Int-PMSG group and Adx-PMSG group and Adx-PMSG group. The rate of ovulation was very low in PMSG-treated groups, but it was increased in 80 to 90% at 104 hours after treatment. However, there was no ovulation in Int-Cont group and Adx-Cont group. 2. Vaginal opening was shown at 56 hours after treatment in Int-PMSG group and Adx-PMSG group and a, pp.ared in 80% at 104 hours after treatment. The rate of vaginal opening in PMSG-treated groups was very low, but Int-Cont group and Adx-Cont group had no vaginal opening. 3. The weight of ovary and uterus in two PMSG-treated groups were increased with the elapse of time after treatment and were significantly heavy in all observation time, but changes in Int-Cont group and Adx-Cont group were not recognized. The weights of ovaries and utera in Adx-Cont group were increased with the elapse of time. 4. The level of serum estradiol-17${\beta}$ was remarkably increased in PMSG-treated groups (Int-PMSG and Adx-PMSG groups) compared with Int-Cont and Adx-Cont group, and significant difference was recognized between Non-PMSG group and PMSG-treated group in the experimental period. Especially, the highest levels of Int-PMSG groups and Adx-PMSG groups were shown at 80 and 56 hours after treatment and after ward estradiol-17${\beta}$ levels of PMSG-treated groups were decreased. However, changes of the levels did not a, pp.ared in Non-PMSG groups at 104 hours after treatment. 5. The level of serum progesterone in PMSG-treated groups was significantly increased between 80 and 104 hours after treatment. With the elapse of time, the level was increased in all observed groups except for Int-Cont and Adx-Conx group. And the order from the highest level at 104 hours after treatment was Int-PMSG, Adx-PMSG, Int-Cont and Adx-Cont group.