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Genetic Characteristics and Anthocyanin Content of Basil(Ocimum basilicum L.)

  • Jae Eun Kim;Song Mun Kim;Ki Yeon Lee;Kyung Dae Kim;Jae Hee Lee;Eun Ha Jang;Jin Gwan Ham
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.318-318
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    • 2022
  • Basil(Ocimum basilicum L.) is an annual herb that grows wild in hot and humid regions such as tropical Asia and Africa. This study was carried out for resource discovery, propagation, and DB construction of aromatic plants. In order to evaluate the genetic characteristics, 28 kinds of basil seeds were supplied from the Center for Genetic Resources. For basil characteristics, planting date, plant height, growth shape, leaf length, leaf shape, petiole color, petiole, stem color, flower color, after harvest, Ml length including roots, flower length, stem thickness and biological weight were measured. For anthocyanin analysis, only basil with purple color was selected, anthocyanins were extracted with 60% ethanol containing 1% citric acid, a standard quantitative curve was prepared with cyanidin-3-glucoside, and absorbance was measured at 525 nm. Basil planting started around June 16, and the flowering period lasted for a total of 18 days from July 19 to August 5. On August 6, when flowering was completed, morphological characteristics including flower color were measured at the site by an object. The measured basil plant length was 26.6-59.6 cm, leaf length 2.0-7.9 cm, leaf width 0.7-5.2 cm, and petiole 1.3-3.9 cm. The growth pattern of basil was generally straight, medium in shape. The color of the leaves was observed evenly by mixed green, purple, purple and green, and the recesses in the shape of the leaf were also conspicuously observed. A total of three basil flower colors were observed: white, light pink(149P 10P/84), and purple(37V 2.5RP 4/12). During the second growth survey after harvest, the total length including the basil roots is 26.7-52.0 cm, the flower length is 8.2-29.3cm, the stem thickness is 7.1-15.9mm, the number of stem nodes is 3-12 nodes, and the total weight of the basil was measured to be about 218-1540 g. There are a total of 16 types of basil expressed in purple, and the anthocyanin content was measured to be 73.74 ~ 380.72 mg/100g.

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Effect of Medium Composition on in vitro Plant Root Regeneration from Axillary Buds of Cassava (Manihot esculenta Crantz) (카사바 액아배양 시 배지조성이 기내 식물체 발근에 미치는 영향)

  • Young Hee Kwon;Won IL Choi;Hee Kyu Kim;Kyung Ok Kim;Ju Hyoung Kim;Yong Sup Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.24-24
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    • 2021
  • The Cassava (Manihot esculenta Crantz) is one of the major food crops in the tropical or subtropical regions. Recently, clean planting materials of improved cassava cultivars are in high demand. Problems in the propagation of cassava are virus vulnerable and low rates of seed germination. Thus, the study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. So we tried to optimize protocols for mass production from axillary buds of Cassava. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with axillary buds, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, axillary buds approximately 1 cm in length were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate showed 55.6%. The shoot number and its length was 1.0/explant and 2.3 cm in the most favorable medium composition. The auxin β-indolebutyric acid(IBA) 0~2.0 mg/L was proved to be effective on root development. Plantlets with fibrous roots easily generated tuberous roots in vitro. The tuberous roots were induced only when both kinetin and IBA were used in combination. after 8 weeks, the root generation rate showed 100%. The root number and its length was 17.2/explant and 2.2 cm in the most promising medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

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Microbiological Quality Evaluation of Foods(Ojingeochaesomoochim : Vegetable Salad with Blanched Squid) That Went through Cooking Process after Heating Treatment in School Food Services (학교급식의 가열조리후처리 음식(오징어채소무침)에 대한 미생물학적 품질 평가)

  • Kim, Yang-Sook;Moon, Hye-Kyung
    • Korean journal of food and cookery science
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    • v.30 no.1
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    • pp.51-63
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    • 2014
  • A microbiological quality evaluation on cooked food, food materials and cooking equipment was conducted in 14 school food services in Changwon area, South Korea for Ojingeochaesomoochim (vegetable salad with blanched squid) which went through cooking process after heating treatment. Only 5 schools out of the 14 (35.7%) observed the sanitization criteria of the Korean School Meals Sanitization Management for raw vegetables (concentration of chlorine solution: 100ppm, immersion time of sanitizing: 5 min). The acceptance rates regarding the microbial standards for Korean school meals showed improvement before and after of sanitization: for onions 57.1% vs 71.4%; cucumber 42.9% vs. 78.6%. However, garlic showed adverse sanitizing effect as the acceptance rate went down from 42.9% to 35.7%. Blanched squid and red pepper paste was acceptable in all 14 schools (100%), while squid seasoned with red pepper powder was acceptable in only 42.9% of the schools. Microbial results for knives, cutting boards, mixing bowls were satisfactory, however 35.7% of sanitary gloves that were put on right before the seasoning showed poor sanitization, and this indicated high probability of cross-contamination from the gloves. We calculated that 57.1% of the cooked foods were in accordance with the microbial standards for Korean school meals. Therefore, it is strongly recommended to have verification process, especially on the effectiveness of sanitization of raw vegetables, in cooking foods through cooking process after heating treatment in HACCP system of school food service.

Analysis of the Contents of Visiting Nursing Articles on Domestic Portal Sites Using Topic Modeling: Focusing on the Comparison Before and After Coronavirus Disease (토픽 모델링을 이용한 국내 포털사이트 방문간호 기사 내용 분석: 코비드-19 이전과 이후 비교를 중심으로)

  • Lim, Ji Young;Lee, Mi Jin;Kim, Geun Myun;Lee, Ok kyun
    • Journal of Home Health Care Nursing
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    • v.30 no.2
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    • pp.141-154
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    • 2023
  • Purpose: This study aimed to explore the social perception of visiting nursing before and after coronavirus disease (COVID-19). Methods: This survey-based study used online big data for comparative analysis by classifying the keywords related to visiting nursing searched on domestic portal sites before and after COVID-19. Results: According to the results of analyzing the Intertopic Distance Map based on Latent Dirichlet Allocation in this study, four topics were extracted, two each before and after COVID-19. The first topic before the COVID-19 period was termed "the expansion of visiting nursing subjects and services visiting nursing," while the second was termed "visiting nursing," which is related to customized welfare. The first topic after the COVID-19 period was termed "the suspension and resumption of visiting nursing services," while the second was "the development of a non-face-to-face home visit healthcare system". Conclusion: The results of this study can be used as useful reference data to contribute to future medical service delivery system reform policies starting at the end of COVID-19 and the revitalization of community care for visiting nursing.

Transitional care for high-risk elderly patients pre/post discharge by collaboration between general hospital and community pharmacy: a pilot study

  • Park, Mi Seon;Lee, Ji Hee;Lee, Heung Bum;Kim, Ju Sin;Choi, Eun Joo
    • Korean Journal of Clinical Pharmacy
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    • v.32 no.1
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    • pp.27-36
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    • 2022
  • Background: Medication-related problems (MRPs) frequently occur during the discharge period. Elderly patients, particularly, are at high risk for these problems due to polypharmacy and the use of potentially inappropriate medications. The purpose of this study was to build and implement collaboration between general hospital and community pharmacies to address MRPs among high-risk elderly patients before/after discharge. Methods: This retrospective study was conducted between June and December of 2020. The inclusion criteria were patients with aged ≥65 years; residents of Jeonju; discharged from Jeonbuk National University hospital; either on medication of exceeding 10 medications (or high-risk medications) after hospitalization through the emergency room, or having severe illness. Patients received medication reconciliation and counselling by hospital pharmacists before discharge and home-visit pharmaceutical care as follow-up by community pharmacists after discharge. Results: Twenty-two patients agreed to home-visit pharmaceutical services. Fifteen and 11 patients completed the first and second home-visit pharmaceutical care service, respectively. Forty-two MRPs were identified in 15 patients. The types of high-frequency MRPs were incorrect administration of drug, adverse drug reactions, medication non-compliance, drug-drug interactions, lifestyle modifications, and expired medication disposal. After consultation with the pharmacist, 34 out of 42 MRPs were resolved. Conclusions: Transitional care for high-risk elderly patients before and after discharge was successfully built and implemented through a collaboration between general hospital and community pharmacies. This study suggests that home-visit pharmaceutical services may have positive effects on the safe use of drugs during the transition period; however, additional research is needed to expand on these findings.

The Effect of Community Service Users' Intention to Repurchase Social Services (지역사회서비스 이용자의 사회서비스 재구매 의도에 미치는 영향)

  • Lee, Hyoung-Ha;Choi, Han-Su
    • Journal of the Korea Society of Computer and Information
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    • v.19 no.9
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    • pp.141-150
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    • 2014
  • This study was conducted as an attempt to explain social service users' intention to repurchase social services. For this purpose, it analyzed variables affecting intention to repurchase social services, focusing on service satisfaction and service effect. The subjects of this study were users participating in community service investment programs and local development projects (city/district) in G Metropolitan City. For the survey, the researcher visited institutions providing social services and had the subjects answer a self-administered questionnaire. A total of 607 questionnaires were recovered and 553 of them were used in the final analysis. The survey period was from the $9^{th}$ to $24^{th}$ of September, 2013. According to the results of analysis, intention to repurchase social services was relatively high as 3.62 out of 5. Service satisfaction was 3.97 out of 5, and service effect was as high as 4.11 out of 5. According to the results of regression analysis on intention to repurchase social services, intention to repurchase services was affected significantly by 'service accessibility' (B=.100, p<.01) among the factors of service satisfaction, and 'More adaptable to daily life after getting services' (B=.206, p<.001) and 'The current services are necessary for my family' (B=.475, p<.001) among the factors of service effect. Based on these results, practical suggestions were made for enhancing users' intention to repurchase social services.

Inhibitory Effect of Purple Corn 'Seakso 1' Husk and Cob Extracts on Lipid Accumulation in Oleic Acid- Induced Non-Alcoholic Fatty Liver Disease HepG2 Model (올레산 유도 비알코올성 지방간세포에서 자색옥수수 색소 1호 포엽과 속대 추출물의 지질 축적 억제 효과)

  • Lee, Ki Yeon;Kim, Tae hee;Kim, Jai Eun;Bae, Son wha;Park, A-Reum;Lee, Hyo Young;Choi, Sun jin;Park, Jong yeol;Kwon, Soon bae;Kim, Hee Yeon
    • Journal of Food Hygiene and Safety
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    • v.35 no.1
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    • pp.93-101
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    • 2020
  • Seakso 1, a maize hybrid, was developed in 2008 by Gangwon Agricultural Research and Extension Services in Korea and registered in 2011. It is single-cross hybrid, semi-flint, deep-purple variety of corn, variety of are yellow, while the husks and cobs are purple. Due to the sensitivity of Seakso 1 to excess moisture after seeding, water supply should be carefully managed, and it should be harvested at a suitable time to obtain the highest anthocyanin content. This study investigated the hepatoprotective effect of Saekso 1 corn husk and cob extracts (EHCS) in oleic acid-induced non-alcoholic fatty liver disease (NAFLD) in HepG2 cells. EHCS showed a high level of lipid accumulation inhibiting effect. EHCS also suppressed triglyceride accumulation and inhibited expression of lipid marker genes, such as sterol regulatory element binding protein-1c (SREBP-1c) and sterol regulatory element binding protein-1a (SREBP-1a). Analysis by western blot of the expression of p-AMPK, p-SREBP1, PPARα, and FAS proteins showed that the incidence of SREBP1 protein, a major factor involved in lipid metabolism in the liver, has decreased significantly after treatment with the extracts. Moreover, the protein-induced expression of FAS, a major enzyme involved in the biosynthetic pathways of fatty acids, was decreased significantly in all concentrations. These results suggest that EHCS is a potent agent for the treatment of NAFLD.

Effects of Different Altitudes and Cultivation Methods on Growth and Flowering Characteristics of Elsholtzia splendens (재배지대와 유형이 꽃향유의 생육 및 개화 특성에 미치는 영향)

  • Young Min Choi;Jin Jae Lee;Dong Chun Cheong;Hong Ki Kim;Hee Kyung Song;Seung Yoon Lee;So Ra Choi;Hyun Ah Han;Han Na Chu
    • Korean Journal of Plant Resources
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    • v.37 no.4
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    • pp.392-400
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    • 2024
  • This study was conducted to find the flowering and growth characteristics according to the different altitudes (plains and mid-mountain regions) and cultivation methods (field and plastic houses cultivation) of Elsholtzia splendens. Experimental regions located at 12 meters and 500 meters above sea level were selected for the plains and the mid-mountain, respectively, and the same method was applied for cultivation management by different altitudes and cultivation methods. In the mid-mountain region, flower bud emergence (2-3 days), flowering (9 days), and full bloom (6-7 days) stages of Elsholtzia splendens were earlier than in the plains, and field cultivation was earlier than plastic house cultivation. The plant height, the main stem diameter, and the number of branches tended to increase gradually after an initial rapid growth at 59 to 69 days after planting date. The days of duration of sunshine (less than 8 hours) from the rainy season (June 20) to the period when vegetative growth increases gradually (59 to 69 days after planting) was 22 to 29 days and 26 to 35 days in the plains and the mid-mountain regions respectively, and this period was estimated time of transition from vegetative growth to reproductive growth. The spikes growth of Elsholtzia splendens by cultivation altitudes was higher in the mid-mountain region than in the plains, and there were no statistically significant differences in growth characteristics except for the main stem diameter, the number of branches, and the dry matter. Also, the amount of flowering and growth was higher in the plastic house cultivation compared to the field cultivation. As a result, some differences in flowering amount were observed when cultivating Elsholtzia splendens for landscaping purposes, but it was considered possible to cultivate in both plains and mid-mountain regions. This study therefore provides ecological information for understanding the relationship between weather characteristics and growth of Elsholtzia splendens.

Pipelining Semantically-operated Services Using Ontology-based User Constraints (온톨로지 기반 사용자 제시 조건을 이용한 시맨틱 서비스 조합)

  • Jung, Han-Min;Lee, Mi-Kyoung;You, Beom-Jong
    • The Journal of the Korea Contents Association
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    • v.9 no.10
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    • pp.32-39
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    • 2009
  • Semantically-operated services, which is different from Web services or semantic Web services with semantic markup, can be defined as the services providing search function or reasoning function using ontologies. It performs a pre-defined task by exploiting URI, ontology classes, and ontology properties. This study introduces a method for pipelining semantically-operated services based on a semantic broker which refers to ontologies and service description stored in a service manager and invokes by user constraints. The constraints consist of input instances, an output class, a visualization type, service names, and properties. This method provides automatically-generated service pipelines including composit services and a simple workflow to the user. The pipelines provided by the semantic broker can be executed in a fully-automatic manner to find a set of meaningful semantic pipelines. After all, this study would epochally contribute to develop a portal service by ways of supporting human service planners who want to find specific composit services pipelined from distributed semantically-operated services.

Pharmaceutical Care Services of Community Pharmacies in Korea Through the Review of Literature (문헌자료 고찰을 통한 우리나라 약국서비스 시행 현황)

  • Sohn, Hyun Soon;Kim, Hyojung;Park, Hyekyung;Han, Nayoung;Oh, Jung Mi;Ji, Eunhee
    • Korean Journal of Clinical Pharmacy
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    • v.25 no.1
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    • pp.18-26
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    • 2015
  • Background: The recent change in pharmaceutical education system following the paradigm shift to patient-oriented pharmacy service requires an in-depth discussion to reorganize a future direction and establish a basis for maximizing social values of community pharmacy service. Objective: This study was conducted to review the current status of community pharmacy service provision in Korea based on published literatures. Methods: The electronic databases of National Digital Science Library and Electronic National Assembly Library were used to search the journal articles and dissertation papers. A search term "community pharmacy" was used and the published period was limited to papers published after year 2001, when the legal separation of prescribing and dispensing was implemented. Relevant study reports were also searched manually. Information about pharmacy service provision and study outcomes were retrieved from the selected papers, and classified by predefined individual service scope. Results: A total 33 papers reporting services provided by community pharmacies were selected (journal article 11, dissertation paper 17, and study report 5). Pharmacy services identified in these papers could be classified into prescription dispensing service, pharmaceutical care service, self medication service, other products service, and health promotion service. Twenty papers reported prescription dispensing services, three papers reported pharmaceutical care service, and only two papers reported health promotion service. Current community pharmacy services are highly dependent on prescription drugs while expanded services such as pharmaceutical care and health promotion are peripheral. Most prevalent research topic was medication counseling service (18 papers), reflecting that community pharmacists generally consider it to be the most important and fundamental service. Overall, current pharmacy services are very limited and focus on prescription dispensing service. Conclusion: At this point of time requiring expansion and quality improvement of community pharmacy services, we suggest further lively discussion to strengthen pharmacist's functional identity and set conditions for providing socially expected services.