• 한국건설관리학회논문집
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• 제22권5호
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• pp.29-38
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• 2021

정부와 기업이 건설업의 산업재해를 줄이기 위해 지속적으로 노력하고 있지만, 재해는 크게 줄어들지 않고 있다. 본 연구는 건설 재해에 영향을 미치는 요인들 간의 연관성을 정량적으로 규명하고자 하였다. 산업안전공단에서 공개한 중대재해 사례 1,197건을 대상으로, 데이터마이닝 기법 중 하나인 연관성 분석을 이용하여 연구를 수행하였다. 산업안전공단에서 제공하는 데이터와 외부 변수를 포함하여 재해 발생 형태, 건설업종, 작업내용, 기인물, 체감온도, 사고 시간대, 추락높이의 변수로 아이템을 구성하여 분석하였으며, 떨어짐 재해와 그 외의 재해로 구분하여 연관규칙을 도출하였다. 떨어짐 재해의 경우 향상도가 1.38 이상인 64개의 연관규칙을 도출하였으며, 떨어짐을 제외한 재해의 경우 향상도가 1.54 이상인 59개의 연관규칙을 도출하였다. 도출된 연관규칙을 재해요인 간의 연관성에 초점을 두고 해석한 후, 고찰에서 연구의 한계와 건설재해 요인 간의 관련성을 파악할 때 연관성 분석 기법을 적용함에 있어 유의사항을 제시하였다. 본 연구는 건설 재해에 영향을 미치는 요인들 간의 연관성을 정량적인 수치로 제시하여 추후 근로자들과 현장관리자가 건설현장에서 적절한 안전대책을 마련하는 기초자료를 제공하였다는 점에서 의미를 찾을 수 있다.

• 한국정보과학회논문지:시스템및이론
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• 제35권4호
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• pp.166-171
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• 2008

전체문서(Corpus)에서의 두 단어 간 연결 상태를 파악하여 앞 단어 다음에 오는 단어의 빈도수를 기반으로 여러 형태의 그룹을 분류하여 단어 간 다양성과 긴밀성을 살펴보았다. 기존의 연구에서 Zipf's Power Law는 Chinese Restaurant Process로 설명되었고 Scale Free Network에서는 edged의 수에 따른 노드의 profile을 조사하여 hub들을 찾는 연구가 수행되었다. 본 연구에서는 단어 간 연결의 유일성과 다양성을 조사하여 Zipf's Power Law와 hub profile을 동시에 살펴보았다. 데이타 분석 결과 단어 간 연결의 긴밀성과 다양성 사이에서 대칭성으로 함축되는 유의한 결과를 얻었으며 이는 소위 'exploitation'과 'exploration'의 관점에서 설명될 수 있다. 또한 분석 자료인 TIPSTER에서 관찰된 약간의 대칭성 깨짐(symmetry breaking)에 대해서도 논한다.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2008년도 학술대회 2부
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• pp.193-198
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• 2008

현재 급속도로 성장하는 인터넷과, PC의 하드웨어, 소프트웨어를 기반으로, 대기업 외에도 중소기업, 소호에서도 대부분의 업무가 네트워크를 활용한 PC 기반으로 연화하고 있다. 하지만 각각의 업무 특성에 고려되지 않은 PC 환경은 사용자들에게 불편함을 야기시키고 이에 따라 각자 환경에 특성화된 PC/Network 서비스의 출현이 예고되고 있다. 본 연구에서는 소호와 중소기업을 대상으로 한 PC 사용자의 이용 행태를 맥락 질문법을 중심으로 이용 행태에 대한 Raw data 를 수집하였고, 분석에서는 Affinity Notes 와 사용자 Modeling 등의 해석 과정을 거쳐, 사용자의 PC/Network 사용 패턴, 환경, 행대 등을 Affinity Diagram 으로 분류화하였다. 분류화된 데이터와 사용자의 Metal Model 을 중심으로 크게 3 가지의 뚜렷한 사용자 이용 행태에 대한 유행을 도출할 수 있었다. 첫 번째 사용자 유행은 업무에 필요하다면 자유롭게 PC 및 네트워크를 사용하여 작업을 수행하는 'Holistic Networker' 이고, 두번째는 자신의 업무에 관해서는 능동적이지만 외적인 부분은 배타적이며 수동적인 'Unplugged Controller' 그리고 마지막 유형으로는 주어진 여건에서 벗어나지 않고 업무를 진행하는 'Passive Reactor'로 분류 할 수 있었다. 이러한 유행은 각각의 성향을 제시할 수 있었고, 이러한 유행과 성향을 바탕으로 PC/Network 기반 시스템제작 시, 서비스 정의, 기능 도출 등에 기초가 될 수 있을 것이라 본다.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.443-451
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• 1994

The binding characteristics of pirenzepine and oxomemazine to muscarinic receptor were studied to evaluate the selectivity of oxomemazine for the muscarinic receptor subtypes in rat cerebral microsomes. Equililbrium dissociation constant $(K_D){\;}of{\;}(-)[^3H]$quinuclidinyl benzilate$([^3H)QNB)$ determined from saturation isotherms was 64-pM. Analysis of the pirenzepine inghibition curve of [$^3H$]QNB binding to cerebral microsome indicatd the presence of two receptor subtypes with high $(K_1 = 16 nM, M_1 receptor)$two receptor subypes with about 20-fold difference in the affinity for high $(k_1 = 84nM, {\;} O_H receptor){\;} and {\;}low{\;} (K_1{\;} ={\;} 1.65\muM, {\;} O_L receptor$) affinity sites. The percentage populations of $M_1{\;} and M_3$, /TEX> receptors to the total receptors were 61 : 39, and those of $O_H{\;} and{\;} O_L$ receptors 39 : 61, resepectively. Both pirenzepine and oxomemazine increaed the $K_D$ value for $[^3H]QNB$ without affecting the binding site concentrations and Hii coefficient for the $[^3H]QNB$ without affecting the binding site concentractions and Hill coefficient for the [$^{3}$H]QNB binding. Oxomemazine had a 10-fold higher affinity at $M_1$ receptors than at $M_3$ receptors, and pirenzepine a 8-fold higher affinity at $O_H$ receptors were of $O_H$ receptors and 71% of $M_3$ receptors. However, $M_3$for oxomemazine and $O_H$for pirenzepine were composed of a uniform population. These results suggest that oxomemazine could be classified as a selective drug for $M_1$ receptors and also demonstrate that rat cerebral microsomes contain three different subtypes of $M_1{\;} M_3$ and the other site which is different from $M_1, {\;} M_2$, /TEX> receptors.

• Bulletin of the Korean Chemical Society
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• 제28권5호
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• pp.758-762
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• 2007

A new alkyldithiocarbonate (xanthate), as sodium salts, C2H5OCS2Na, was synthesized by the reaction between CS2 with ethyl alcohol in the presence of NaOH. The new xanthate was characterized by 1H NMR, IR and elemental analysis. Then, the new synthesized compound was examined for functional study of cresolase activity of Mushroom Tyrosinase (MT) from a commercial source of Agricus bisporus in 10 mM phosphate buffer pH 6.8, at three temperatures of 10, 20 and 33℃ using UV spectrophotemetry. 4-[(4-methylphenyl)- azo]-phenol (MePAPh) was used as a synthetic substrate for the enzyme for cresolase reaction. The results show that ethyl xanthate can activate or inhibit the cresolase activity of mushroom tyrosinase depending to the concentration of ethyl xanthate. It was concluded that the enzyme has two distinct sites for ethyl xanthate. The first one is a high-affinity activation site and the other is a low-affinity inhibition site. Activation of the enzyme in the low concentration of ethyl xanthate arises from increasing the affinity of binding for the substrate as well as increasing the enzyme catalytic constant. The affinity of ligand binding in the activation site is decreased by increasing of the temperature, which is the opposite result for the inhibition site. Hence, the nature of the interaction of ethyl xanthate is different in two distinct sites. The binding process for cresolase inhibition is only entropy driven, meanwhile the binding process for cresolase activation is not only entropy driven but also enthalpy driven means that hydrophobic interaction is more important in the inhibition site.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권6호
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• pp.402-409
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• 2001

Different bioligands carrying synthetic adsorbents have been reported in the literature for protein separation, We have developed a novel and new approach to obtain high protein ad-sorption capacity utilizing 2-methacrylamidohistidine(MAH) as a bioligand. MAH was synthe-sized by reacting methacrylocholride and histidine, Spherical beads with an average size of 150-200㎛ were obtained by the radical suspension polymerization of MAH and 2-hydrosyethyl-methacrylate(HEMA) conducted in an aqueous dispersion medium. p(HEMA-co-MAH) beads had a specific surface area of 17.6㎡/g . Synthesized MAH monomer was characterized by NMR. p(HEMA-co-MAH) beads were characterized by swelling test, FTIR and elemental analysis. Then Cu(II) ions were incorporated onto the beads and Cu(II) loading was found to be 0.96 mmol/g.These affinity beads with a swelling ration of 65% and containing, 1.6 mmol MAH/g were used in the adsorption/desorption of human serum albumin(HSA) from both aqueous solutions and hu-man serum. The adsorption of HSA onto p(HEM-co-MAH) was low(8.8 mg/g). Cu(II) chelation onto the beads significantly increased the HSA adsorption (56.3 mg/g). The maximum HSA ad-sorption ws observed at pH 8.0 Higher HSA adsorption was observed from human plasma(94.6 mgHSA/g) Adsorption of other serum proteins were obtained as 3.7 mg/g for fibrinogen and 8.5mg/g for γ-globulin. The total protein adsorption was determined as 107.1mg/g. Desorption of HSA was obtained using 0.1 M Tris/HCl buffer containing 0.5 M NaSCN, High desorption rations(up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cu(II) chelated-p(HEMA-co-MAH) beads without significant decreases in the adsorption capacities.

• 생명과학회지
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• 제18권4호
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• pp.488-493
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• 2008

Phosphate, a favorable phosphorous form for plant, is one of major nutrient elements for growth and development in plants. Plants exhibit various physiological and biochemical responses in reaction to phosphate starvation in order to maintain phosphate homeostasis. Of them, expression of high affinity phosphate transporter gene family and efficient uptake of phosphate via them is a major physiological process for adaption to phosphate deficient environment. Although the various genetic resources of high affinity phosphate transporter are identified recently, little is known about their functions in plant that is prerequisite information before applying to crop plants to generate valuable transgenic plants. We demonstrated that Arabidopsis transgenic plants over-expressing two different high affinity phosphate transporter gens, OsPT1 and OsPT7, derived from rice, exhibit better growth responses compared with wild-type under phosphate starvation condition. Specially, OsPT7 gene has proven to be more effective to generate Arabidopsis transgenic plant tolerant to phosphate deficiency than OsPT1. Furthermore, the expression level of AtPT1 gene that is one of reporter genes specifically induced by phosphate starvation was significantly low compared with wild-type during phosphate starvation. Taken together, these results collectively suggest that over expression of OsPTl and OsPT7 genes derived from monocotyledonous plant function efficiently in the dicotyledonous plant, relieving stress response caused by phosphate starvation and leading to better growth rate.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2009년도 추계학술대회 및 정기총회
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• pp.14-16
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• 2009

Poria cocos is an importantant medical macrofungus,the sclerotium of Poria cocos has specific value as the drug material. There are few papers about its breeding and spawn rejuvenation. In this project, the protoplasts of cultivated strain T and wild strain L were prepared and treated separately by ultraviolet and heating, then fused with the PEG6000. The tural fusants were selected and identified by the affinity and ISSR analysis. 71 incompatibility strains between parents and reg regenerations were obtained from 118 regenerations by the affinity analysis. Five incompatibility strains were amplified with different primers, the results were showed that they had specific bands of both parents in the profile amplified with 3 primers, which proved these 5 strains were fusants by means of molecular biology marker. On the other hand, 25 strain were selected from 168 protoplast regenerations of cultivated strain T for cultivation experiment. The fresh sclerotium weight of these protoplast regenerations were better than the original strain.significantil 3 strains (T-1, T-4, T-7) increased respectively 118%, 73% and 73% than original strain. This method could be the effective in the rejuvenation Poria cocos.

• 대한건축학회논문집:계획계
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• 제35권1호
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• pp.83-92
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• 2019

Advertising signboards are designed for the visibility to affects recall and recognition of costumers. It is well know that the visibility from images is created by the value difference among colors. The research defines whether the background color combination of outdoor signboards is configured to maximize visibility, by a series of color value variation in complex commercial facilities. The subject of study is to examine how the visibility is made by the color combination since visibility cannot be obtained independently. Two steps of analysis were performed to confirm that the color composition of signboards was based on the color value difference. The first is to analyze that the entire colors of signboards are clearly categorized as different value groups. All components of colors, hue, value and chroma had been analyzed by color aesthetic measures to prove that the value variation has the only regularity and the principle of composition. The second step is a further verification with an ample amount of samples to determine whether series of signboards create a value altering pattern. The data for analysis is gained by colorimetric survey and the color data are used for exponentializing the degree of combining, which shows selective affinity between each pair colors.

• BMB Reports
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• 제40권5호
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• pp.723-730
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• 2007

Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.