• Journal of Korean Society of Coastal and Ocean Engineers
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• v.19 no.2
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• pp.179-182
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• 2007

In this study hydraulic model experiment was conducted to identify the performance of newly developed water affinity sea-wall block to keep the coastal line from eroding and supply water affinity space by reflection coefficient and safety. In the result, the block is applicable to the field.

• Journal of environmental and Sanitary engineering
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• v.12 no.2
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• pp.59-64
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• 1997

For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with protein A-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na$_{2}$HPO$_{4}$ buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.145-145
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• 1993

일반적으로 단백질의 정제를 위한 가장 효과적인 방법은 affinity chromatography법으로 알려져 있다. 본 연구에서는 베타 수용체의 순수정제를 위한 효과적인 affinity matrix를 제조하고 이의 특징을 확인함으로써 수용체 정제를 위한 기초를 수립하고자 하였다. Bisoxirane reagent인 1,4-butanediol diglycydyl ether를 Sepharose CL-4B gel과 반응시켜 탄노원자 10개 길이의 spacer arm을 부착시켰다. 여기에 sodium persulfate, dithiothreitol을 연속적으로 사용하여 -SH기를 도입시긴 추 free radical 반응을 이용하여 베타수용체 길항제인 alprenolol을 부착시킴으로써 affinity gel을 제조하였다. alprenolol치 부착반응을 위한 최적온도는 4$0^{\circ}C$로 나타났으며, semimicro Kjeldahl 질소 분석법을 적용하여 gel을 분석한 결과 ml당 0.4-0.6 $\mu$ mol의 alprenolol이 결합하였음을 확인하였다.

• YAKHAK HOEJI
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• v.41 no.5
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• pp.638-646
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• 1997

The steroid 9${alpha}$-hydroxylase activity has been detected in cytosol fraction, $100,00{\times}g$ supernatant of cell free extract of Mycobacterium fortuitum. The activity was not linear with protein concentration in the assay suggesting 9${alpha}$-hydroxylase is a multicomponent enzyme. The 9${alpha}$-hydroxylase system was partially purified through fractional saturation of ammonium sulfate, strong anion exchange (Mono Q) column chromatography, gel filtration (Superose 12) column chromatography, and testosterone affinity gel chromatography. Ammonium sulfate 50~60% saturated fraction of the cytosol gave 9${alpha}$-hydroxylase activity. For further purification, the half-saturated ammonium sulfate fraction was applied to Mono Q, Superose 12, or affinity gel column. The purification factors of 9${alpha}$-hydroxylase containing fraction after Mono Q, Superose 12, and affinity gel chromatography was 13, 11, and 17 respectively.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.1051-1054
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• 2008

The entropy production in a non-equilibrium state based on the reversible Oregonator model of the Belousov-Zhabotinskii (BZ) reaction system has been studied. The reaction affinity and the reaction rate for the individual steps have been calculated by varying the concentrations of key variables in the system. The result shows a linear relationship between the reaction affinity and the reaction rate in the given concentration range. However, the overall entropy calculated on the basic assumption that the entropy in a reaction system corresponds to the summation of a product of reaction affinity and reaction rate of individual steps shows a nonlinearity of the reaction system. The results well agrees with the fact that the entropy production is not linear or complicated function in a non-linear reaction system.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.4
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• pp.240-245
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• 2003

The use of microfabricated microfluidic devices offers significant advantages over current technologies including fast analysis time and small reagent requirements. In the context of proteomic research, the possibility of using affinity-based separations for prefractionation of samples using microfluidic devices has significant potential. We demonstrate the use of microscale devices to achieve affinity separations of proteins using a device fabricated from borosilicate glass wafers. Photolithography and wet etching are used to pattern individual glass wafers and the wafers are fusion bonded at 650$^{\circ}C$ to obtain enclosed channels. A polymer has been successfully polymerized in situ and used either as a frit for packing beads or, when derivatized with Cibacron Blue 3GA, as a separation matrix. Both of these technologies are based on in situ UV photopolymerization of glycidyl methacrylate (GMA) and trimethylolpropane trimethacrylate (TRIM) in channels.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.204-206
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• 2012

SMP(Symmetric Multi-Processing)는 Shared memory bus 를 사용함으로써 scalability 가 제한적이었다. 이런 SMP의 scalability 제한을 극복하기 위해 제안 된 것이 NUMA(Non Uniform Memory Access)이다. NUMA는 memory bus 를 CPU 별 local 하게 가지고 있어 자신이 가지는 memory 영역에 대해서는 다른 영역을 접근하는 것 보다 더 빠른 latency 를 가지는 구조이다. Local 한 memory 영역의 존재는 scalability를 높여 주었지만 서버 가상화 환경에서 VM을 동적으로 scheduling 을 하였을 때 VM의 page 가 실행되는 core 의 local 한 메모리 영역에 존재하지 않게 되면 remote access로 인해 local access보다 성능이 떨어진다. 이 논문에서는 서버 가상화 환경에서 최신 architecture인 AMD bulldozer에서 NUMA affinity가 위반되었을 때 발생하는 성능 저하와 어떤 상황에서 이런 NUMA affinity가 위반되어도 성능저하가 없는지 연구하였다.

• Asian Journal for Public Opinion Research
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• v.11 no.2
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• pp.107-144
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• 2023

In competitive political contexts, sustaining power is the ultimate goal for political parties. Nominating celebrity politicians can be a double-edged sword for parent brands in attracting votes and influencing voting intention. This study contributes to the moderating role of brand affinity towards celebrity politicians. It considers celebrities' cognitive perceived benefits and voting intention relationship in a multiparty parliamentarian election. A cross-sectional survey with a stratified proportional random sampling technique in fifteen Lebanese districts ensured a representative sample. One thousand two hundred sixty-nine responses were found eligible for analysis. Findings indicate that brand affinity positively moderates the negative relationship between perceived benefits and voting intention. This study offers a new understanding of celebrity politicians' implementation strategy and campaign management and considers the contribution of the affective intelligence theory. It provides implications, limitations, and promising directions for future research on celebrity politicians.

• IMMUNE NETWORK
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• v.16 no.4
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• pp.219-232
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• 2016

Production of high affinity antibodies for antigens is a critical component for the immune system to fight off infectious pathogens. However, it could be detrimental to our body when the antigens that B cells recognize are of self-origin. Follicular helper T, or Tfh, cells are required for the generation of germinal center reactions, where high affinity antibody-producing B cells and memory B cells predominantly develop. As such, Tfh cells are considered as targets to prevent B cells from producing high affinity antibodies against self-antigens, when high affinity autoantibodies are responsible for immunopathologies in autoimmune disorders. This review article provides an overview of current understanding of Tfh cells and discusses it in the context of animal models of autoimmune diseases and allograft rejections for generation of novel therapeutic interventions.

• Journal of the Korean Chemical Society
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• v.59 no.1
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• pp.93-96
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• 2015