• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.501-510
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• 2007

The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes involved in muscle cell proliferation and differentiation. Of three different types of proteases (type II collagenase, pronase and trypsin-EDTA) used to hydrolyze the myogenic satellite cells from muscle tissues, trypsin-EDTA treatment yielded the highest number of cells. The cells separated by hydrolysis with type II collagenase and incubated on gelatin-coated plates showed an enhanced cell attachment onto the culture plate and cell proliferation at an initial stage of cell growth. In this study, the bovine MSCs were maintained in vitro up to passage 16 without revealing any significant morphological change, and even to when the cells died at passage 21 with decreased or almost no cell growth or deformities. When the cells were incubated in a steroid-depleted environment (DMEM(-)/10% CDFBS (charcoal-dextran stripped FBS)), they grew slowly initially, and were widened and deformed. In addition, when the cells were transferred to an incubation medium containing steroid (DMEM(+)/10% FBS), the deformed cells resumed their growth and returned to a normal morphology, suggesting that steroid hormones are crucial in maintaining normal MSC morphology and growth. The results demonstrated that treatments with 19-nortestosterone and testosterone significantly increased AR gene expression (p<0.05), implying that both testosterone and 19-nortestosterone bind with AR and that the hormone bound-AR complex up-regulates the genes of its own receptor (AR) plus other genes involved in satellite cell growth and differentiation in bovine muscle.

• Journal of Periodontal and Implant Science
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• 제35권1호
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• pp.111-121
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• 2005

Coronary heart disease is the leading cause of mortality in adult population. Whereas the association between periodontal disease and coronary heart disease (CHD) are controversial, recent studies reported the association between periodontal disease and acute myocardial infarction or prognosis of CHD. This study was aimed to investigate the relationship between periodontal disease and angiographically defined CHD, and acute myocardial infarction, and the prognosis of treated CHD. Patients under the age of 60 who had undergone the diagnostic coronary angiography were enrolled in this study, Subjects were classified as positive CHD (+CHD, n=37) with coronary artery stenosis more than 50% in at least one of major epicardial arteries, and negative CHD (-CHD, n=20) without stenosis. After recording the number of missing teeth, periodontal disease status was measured by means of plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), and clinical attachment level (CAL), Positive CHD subjects were classified into acute myocardial infarction group (AMI), and non-AMI with angina pectoris and old myocardial infarction. Six months postoperatively, positive CHD subjects were followed and had undergone the coronary angiography again. Even though there was no significant difference in the periodontal parameters and status between positive CHD and negative CHD, some periodontal parameters, such as mean probing depth and proportion of sites with probing depth greater than 4mm or 6mm were significantly different between AMI and Non-AMI(p<0.05). There was no significant difference in the periodontal parameters according to in angiographically follow-up status. These results indicate that periodontal disease may be associated with the occurrence of acute myocardial infarction.

• Journal of Periodontal and Implant Science
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• 제24권1호
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• pp.87-97
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• 1994

The ultimate goal of periodontal therapy is to fully reconstruct the periodontal attachment apparatus. Commonly used techniques for treatment of infrabony defects include a combination of root planing, curettage and root treatment. To prevent the apical migration of epithelial cells, the technique of guided tissue regeneration is used. The aim of this study is to compare the effects of root treatment with Citric acid & Tetracycline and Guided tissue regeneration in dogs. Experimental periodontitis was induced by the ligation of orthodontic elastic threads in the upper right and left premolars 3, 4 of five adult dogs for 6 weeks. 4 types of procedures were performed as follows; 1) Control graup : Mucoperiosteal flap 2) Experinental I : GTR used Gore-tex(R) membrane 3) Experinental II : Root treatment with citric acid (PHl) 4) Experinental III : Root treatment with tetracycline HCl (50mg/ml) There after, dogs were serially sacrificed at the 1, 2, 4, 5, 8 weeks, and the specimens were prepared, and stained with hematoxylin-eosin for the light microscopic evaluation. The results of this study were as follows; 1. Junctional epithelium reached to the notch through the furcation area in control group at 8 weeks. 2. In the aspects of the inflammatory cell infiltration, control group showed severe aggregation than experimental group I, II, III through the experimental period 3. New cementum was observed over the notch from 5 weeks in experimental group II 4. In the aspects of the amount of new bone formation, experimental group was better than control group, but there was not significant differences among the experimental group, I, II, III

• Imaging Science in Dentistry
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• 제41권3호
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• pp.115-121
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• 2011

Purpose : This article is to describe a modified device for intraoral radiography which was developed to obtain reproducible radiographic images for assessment of distal osseous defects of the mandibular second molar (2 Mm) after impacted third molar (3 Mm) surgery. Materials and Methods : A commercial available alignment system for posterior region was modified by adding a reference gauge pin (millimetric) and threading a hollow acrylic cylinder at the ring of the radiographic positioner to attach the X-ray collimator. The design included customized resin acrylic stent for the occlusal surface of the 2Mm in maximum intercuspal position, individualizing the biteblock positioner. Periapical radiographs were taken before and after surgical extraction of 3 Mm, employing the radiographic technique of parallelism described by Kugelberg (1986) with this modified film holder and inserting the gauge pin on the deepest bone probing depth point. Results : This technique permitted to obtain standardized periapical radiographs with a moderate to high resolution, repeatability, and accuracy. There was no difference between the measurements on the pre- and post-operative radiographs. This technique allowed better maintenance of the same geometric position compared with conventional one. The insertion of the gauge pin provided the same reference point and localized the deepest osseous defect on the two-dimensional radiographs. Conclusion : This technique allowed better reproducibility in posterior radiographic records (distal surface of 2 Mm) and more accurate measurements of radiographic bone level by the use of a millimetric pin.

• 대한의용생체공학회:의공학회지
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• 제44권1호
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• pp.80-84
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• 2023

Electrodes are one of the types of biosensors capable of measuring bio signals, such as electrocardiogram (ECG) and electromyogram (EMG) signals. These electrodes are used in various fields and offer the advantage of being able to measure ECG signals without the need for skin attachment, compared to Ag/AgCl electrodes. The purpose of this study was to evaluate the efficacy of conductive textile electrodes in collecting ECG signals in a bed-like environment. Three adult participants were involved, and a total of 30 minutes of ECG signals were collected for each participant. The collected ECG signals were analyzed to determine the heart rate, normLF and a comparison was made between the conductive textile electrodes and Ag/AgCl electrodes. As a result, the change in heart rate and normLF could be observed, and in particular, the difference between the two electrodes decreased. This study confirmed that conductive textile electrodes can effectively collect ECG signals in a bed-like environment. It is hoped that this research will lead to the development of a system that can detect various sleep-related diseases through the use of these electrodes.

• 한국가정과교육학회지
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• 제29권1호
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• pp.71-92
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• 2017

본 연구는 30-40대 싱글여성의 원가족 및 사회적 관계의 경험을 심층면접을 통해 분석하였다. 서울경기, 경상도와 전라도 지역에 거주하는 싱글여성 18명을 대상으로 반구조화된 심층 면담을 실시하였다. 연구자들은 면담의 축어록을 통해 참여자들의 원가족과 사회적 관계에서의 경험과 관련된 진술문만을 발췌하여 분석하였고, 수집한 자료들은 주제별 분석법을 적용하여, 면접 내용의 부호화 과정, 해체화 과정, 재구성 과정의 3단계로 진행하였다. 그 결과 '가족 애착', '특별한 사람들', '소원한 친인척 관계', '작은 사회생활', '만남의 상실', '무엇보다 자유를 찾아' 라는 6가지 대주제를 찾을 수 있었다. '가족 애착'이라는 대주제에서는 "돌보아지는 성인아기, 싱글생활에 대한 지지, 끈끈한 가족관계"의 3개 중주제를 찾았다. '특별한 사람들'이라는 대주제에서는 "엄마 사랑, 친구같은 언니, 사랑스러운 조카"의 3개 중주제를 찾았고, '소원한 친인척관계'는 하나의 단일 중범주를 찾을 수 있었다. 또한 '작은 사회생활'에서는 "직장에서의 인연, 지인들과의 담소, 이성과 거리두기, 싱글은 싱글끼리"의 4개 중주제를, '만남의 상실'이라는 대주제에서는 "만남보다 홀로, 귀차니즘, 벅찬 일상"의 3개 중주제를, '무엇보다 자유를 찾아'라는 대주제에서는 "애착 주의, 얽힘 주의"의 2개 중주제를 찾아서, 총 16개의 하위 범주를 찾았다. 이와 같은 주제 분석의 결과는 싱글 여성들이 나타내는 원가족과 사회적 관계에서의 독특한 경험의 특성으로 볼 수 있어서, 이같은 결과가 싱글 여성의 삶에 대한 이해의 폭을 확장시켰다고 볼 수 있다. 싱글여성들이 보이는 끈끈한 가족관계와 스스로 선택한 좁은 사회적 관계 형성의 특성을 고려하면, 생애설계교육에서는 부모와 자녀간의 건강한 애착 형성을 위한 교육과 다양한 사람들과 건강한 관계를 형성하도록 지원할 수 있는 교육내용이 중요하게 다루어져할 것으로 보인다. 즉 결혼과 가족생활에 대한 새로운 가치를 발견하도록 지원하는 인구교육에서는 좋은 부모가 되기 위한 부모교육과 다양한 인간들과 건강한 관계를 형성하도록 지원하는 교육내용이 중요한 교육내용으로 다루어져야 함을 시사하였다.

• 대한골관절종양학회지
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• 제3권1호
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• pp.18-25
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• 1997

Prosthetic replacement is one of the most common methods of reconstruction after resection of malignant tumor around the knee. Gait analysis provides a relative objective data about the gait function of patients with prosthesis. The purpose of this study was to compare the gait pattern of the patients who underwent limb salvage surgery with prosthesis for distal femur and that of patients with prosthesis for proximal tibia. This study included ten patients (4 males, 6 females, mean age 22.7 years, range 14-36) who underwent a wide resection and Kotz hinged modular reconstruction prosthesis replacement and six normal adult(Control). The site of bone tumor was the distal femur (Group 1) in six patients and proximal tibia (Group 2) in 4 patients. The follow-up period ranged from 15 to 82 months (mean : 33 months). The evaluation consisted of clinical assessment, radiographic assessment, gait analysis using VICON 370 Motion Analysis System. The gait analysis included the linear parameters such as, walking velocity, cadence, step length, stride length, stance time, swing time, single support and double support time and the three-dimensional kinematics (joint rotation angle, velocity of joint rotation) of ankle, knee, hip and pelvis in sagittal, coronal and transverse plane. For the kinetic evaluation, the moment of force (unit: Nm/kg) and power (unit: Watt/kg) of ankle, knee and hip joint in sagittal, coronal and transverse plane. In the linear parameters, cadence, velocity, step time and single support were decreased in both group 1 and group 2 compared with control. Double support decreased in group 2 compared with control significantly(p<.05). In contrast to our hypothesis, there was no significant difference between group 1 and group 2. In Kinematics, we observed significant difference (p<.05) of decreased knee flexion in loading response (G2

• 대한치과교정학회지
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• 제29권4호
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• pp.445-456
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• 1999

최근에는 교육수준의 향상, 소득증대, 대중매체의 발달 등으로 인하여 성인 교정환자의 수요가 상당히 증가하고 있다. 성인환자는 어린환자에 비해 상대적으로 구강내 도재수복물을 장착하고 있는 경우가 많아 도재치에 대한 교정용 브라켓의 직접접착의 기회도 증가하고 있다. 자연치에서는 법랑질을 산부식하여 비교적 쉽게 접착력을 증진시킬 수 있으나 도재치에서는 임상에서 브라켓이 너무 쉽게 탈락하거나 오히려 도재파절의 위험성이 존재하여 도재치에 대한 효과적인 직접접착법에 대하여 관심을 갖게 되었다. 그러나, 이러한 접착효율에 대한 다양한 연구가 있었으나 아직 정설로 받아들여지는 것은 없는 실정이다. 본 연구는 임상에서 비교적 간단히 이용할 수 있는 몇 가지 방법을 in vitro 상의 실험을 통하여 그 결과를 비교검토하여 효과적인 방법을 제시하고자 시행되었다. 우선 장석형 도재인 Ceramco II로 도재 시편을 제작하였다. 도재의 표면처리방법은 Glazing한 도재 자체를 사용하기 위해서는 무처리하였으며, 거칠기를 부여하기위해서는 불화수소산으로 산부식하거나 Microetcher II로 Sandblasting, 또는 불화수소산과 Sandblasting을 복합처리하였다. 접착레진은 화학중합형 인 Ortho-two와 광중합형인 Transbond를 사용하였다 모든 시편에는 도재 프라이머를 도포하였다. 실험군은 7가지 군으로 나누어 브라켓의 전단강도를 측정하였다. 1. 도재의 표면처리에 대한 비교에서 Ortho-two군내에서는 불화수소산이나 sandblasting으로 표면거칠기를 부여한 군이 무처리군에 비해 높은 전단강도를 갖는다. Transbond군내에서는 각각의 표면처리에 따른 유의성있는 차이가 없었다. 2. 불화수소산이나 Sandblasting처리군에서는 화학중합형 레진인 Ortho-two가 광중합형 레진인 Transbond보다 높은 전단강도를 갖는다. 3. 도재 파절율의 비교에서는 무처리군이 표면거칠기를 부여한 군에 비해 매우 낮은 파절율 갖는다. 4. E(Transbond+Intact)군의 평균전단강도는 134.4kg/$cm^2$ 로 본 실험군중 가장 낮은 것으로 나타났다. 이것은 선학들이 제안한 임상적용가능 전단강도보다 크므로 7가지군 모두 임상적으로 교정력을 가하기 에 충분한 전단강도를 갖는다. 그러나, 도재의 파절율을 고려해볼 때 파절율이 상대적으로 낮은 무처리군이 임상적으로 더 효과적일 것으로 사료된다.

• Journal of Chest Surgery
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• 제40권4호
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• pp.288-291
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• 2007

배경: 심장 내의 점액종은 심장 내 모든 종양의 약 반수를 차지하며 그 중 75%는 좌심방 내에 발생하나 우심방, 드물게는 심실 내 점액종도 보고되고 있다. 대개의 증상은 혈행장애나 색전에 의한다. 진단은 다른 심질환을 합병하지 않는 한 심초음파만으로 가능하며 치료는 조기에 수술적인 제거로 거의 완치가 가능하다. 대상 및 방법: 1984년 7월부터 2004년 7월까지 심장 점액종을 수술한 환자 57명을 대상으로 의무 기록 검토를 통한 후향적 분석을 시행하였다. 걸과: 환자의 연령은 12세에서 76세 사이로 평균 $53.5{\pm}14.0$세였고 여자가 38명(67%), 남자가 19명(33%)이었다. 술 전 증상으로는 운동 시 호흡곤란이 27예에서 있었고, 심계항진이 4예, 흉통이 9예, 실신을 경험한 경우가 4예 있었다. 진단방법으로 6예에서 심장초음파와 CT 및 혈관 조영술이 진단에 이용되었으며 나머지 51예에서는 심장초음파검사만으로 충분하였다. 수술 소견상 점액종의 부착 부위는 심방중격이 50예, 승모판막윤이 3예, 좌심방벽이 4예였으며 점액종의 크기와 형태는 다양하였다. 점액종을 제거하기 위한 접근방법으로 3예에서는 우심방 절개를 통한 중격절제를, 33예(58%)에서는 양심방 절개를 시행하였으며, 좌심방을 통해 15예(26%), inverted T incision을 통해 3예, 확장된 중격절제를 통해 3예를 수술하였다. 총 추적기간은 1개월부터 229개월까지로 평균 $84.0{\pm}71.3$개월이었고, 추적 관찰 도중 손실된 5명을 제외하고 수술로 인한 조기 및 만기 사망 예는 없었으며 외래 추적 중 재발도 없었다. 결론: 심장점액종은 판막질환이나 관상동맥질환 등 다른 질환 등과 구별하기 어려운 임상적 증상을 보이면서 색전에 의한 사망률 및 이환율이 높고 재발하는 경우도 있으며 진단과 동시에 특별한 부적응증이 없는 한 수술로써 근치가 가능하기 때문에 정확한 진단과 함께 보다 완벽한 절제가 요구된다.

• 지역사회간호학회지
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• 제15권4호
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• pp.587-599
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• 2004

Purpose: The purpose of this study is to compare abdomen-fat rate, life style and social-support between normal weight women and obese women. Method: 304 women objectives from their 30 to 59 years of age were selected living in Je-chon city, Chung-Buck province and their height and weight were measured from April 1st to June 30th, 2003. Data were classified into low-weight group ($18.5kg/m^2$), normal-weight group ($18.5{\sim}22.9kg/m^2$), over weight group ($23{\sim}24.9kg/m^2$), and obese group ($25kg/m^2$) following the Korean Conference of Obesity, 2001. in which 119 people in the normal weight group and 91 people in the obese group, i.e. total 210 people were analyzed in sequence. Using SPSS Win 10.1 Program, frequency and percentile, and by ANOVA, $X^2-test$ and t-test were treated. Results: The average age of obese women was 46.68 distributing 40.7% of forties and 39.6% of fifties while normal-weight women were average 41.73-year old distributing 53.8% of forties and 34.5% of thirties, which revealed aged in obese women. The body fat rate of obese women averaged $37.52{\pm}4.17%$, in which 98.9% of obese women and 21.0% of normal weight women with a more than 30% of body-fat rate resulted in a higher body-fat rate in obese women. The waists of obese women averaged $88.37{\pm}8.22cm$, in which more than 85cm showed in obese women of 68.2% and normal weight women of 7.6% indicating a higher waist-fat rate in obese women. The abdomen-fat rate of more than 0.85 of waist vs hip-fat showed 74.7% in obese women and 58.4% in normal weight women, indicating a higher abdomen-fat rate in obese women. Obese women and normal weight women showed significant differences in education level, number of children, religion, menstrual status, and mother's weight. Especially, obese women ate hotter or saltier food than normal weight women preferring meat. However, no significant differences appeared in marital status, social economic status. occupation. eating habits. smoking. drinking and physical exercise. Social support levels showed a lower rate in obese women than in normal weight women, indicating a statistically significant difference (p<.05). Observing areas of social support, obese women showed lower rates in attachment/intimacy, social integrity, opportunity of foster and confidence in value except help and instruction, which indicated a statistically significant difference (p<.05). Social support for obese women showed significant differences in age, education level, social hierarchy, religion and menstrual status. Obese women were more negative than normal weight women in health recognition, indicating a statistically significant difference (p<.01). Normal weight women showed higher health recognition when provided high social support and significantly low (p<.01) health recognition when provided low social support. However, there was no significant difference in health recognition in obese women whether high or low social support was given. The health recognition of obese women showed significant differences in age, education level, social hierarchy, number of children, menstrual status, physical exercise, eating habits, eating taste and preference of food. Conclusion: Obese women showed elder than normal-weight women, higher body-fat rate and abdomen-fat rate, lower social support, and a tendency to more negative health recognition. Therefore, providing weight-control programs for the treatment of obesity and prevention of recurrence for obese women to prevent progressing to adult disease and promote a healthy life, we suggest that better eating habits and the encouragement of regular physical exercise should be included, as well as total approachment on change of health recognition and social support would be needed.