• 대한본초학회지
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• 제22권2호
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• pp.121-126
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• 2007

Objectives : A morphological classification among Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora and Codonopsis pilosula was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Codonopsis pilosula is like a long cone, with amole-like projected parts at the beginning of the roots. 2. Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora, and Codonopsis pilosula are all similar as following: 1) Cork layers are composed of a few to over 10 subsidiary cells, with individual or a group of stone cells among them. 2) Phloem is wide and has a milk tube. Conclusion : Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora, and Codonopsis pilosula are all similar and difficult to be distinguished through microscopic observation, so that chemical experiments should be conducted for their identification.

• 동아시아식생활학회지
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• 제27권4호
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• pp.442-449
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• 2017

Adenophora triphylla var. japonica extract has been reported to have estrogenic activity and beneficial effects in postmenopausal women requiring estrogen replacement therapy. This study investigated the effects of A. triphylla extract on cognitive function in ovariectomized mice. Water extracts from A. triphylla were administrated to ovariectomized mice fed a high fat diet for 6 weeks. The step through latency time in the passive avoidance test was shortened in ovariectomized mice as compared with sham-operated mice. However, administration of A. triphylla extracts had a tendency to prolong the latency time in ovariectomized mice. Moreover, A. triphylla supplementation caused a significant decrease in mean escape latency in the water maze test. These results indicate that A. triphylla could be used to improve estrogen deficiency-related learning and memory impairments in postmenopausal women.

• Journal of Plant Biotechnology
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• 제50권
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• pp.137-141
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• 2023

잔대(Adenophora triphylla var. japonica)의 뿌리는 비만, 암, 염증을 예방하는 데 사용되는 귀중한 약용 자원으로 사용되지만, 어린잎이나 순은 식재료로 사용되고 있다. 본 연구에서는 A. triphylla 뿌리와 잎에서 얻은 70% 에탄올 추출물의 항산화 및 항암 효과를 비교 평가하였다. 잎 추출물이 뿌리 추출물보다 강력한 DPPH-radical 소거 활성, 환원력 및 ORAC 값을 나타냈다. 또한, 인체 암세포주에서 잔대 추출물의 세포독성 사멸 효과를 조사한 결과, 잎 추출물이 A549 (폐암), LNcaP (전립선암), SKOV3 (난소암) 및 Caco-2 (대장암) 세포에서 강력한 항암 성분의 공급원임을 보여주었다. 이러한 결과는 뿌리뿐만 아니라 잎도 제약 산업에서 기능성 소재의 귀중한 공급원이 될 수 있음을 시사한다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.437-438
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• 2010

• Tuberculosis and Respiratory Diseases
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• 제78권3호
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• pp.210-217
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• 2015

Background: Adenophora triphylla var. japonica is empirically used for controlling airway inflammatory diseases in folk medicine. We evaluated the gene expression and production of mucin from airway epithelial cells in response to lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica. Methods: Confluent NCI-H292 cells were pretreated with lupenone, lupeol or taraxerol for 30 minutes and then stimulated with tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$) for 24 hours. The MUC5AC mucin gene expression and production were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Additionally, we examined whether lupenone, lupeol or taraxerol affects MUC5AC mucin production induced by epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA), the other 2 stimulators of airway mucin production. Results: Lupenone, lupeol, and taraxerol inhibited the gene expression and production of MUC5AC mucin induced by TNF-${\alpha}$ from NCI-H292 cells, respectively. The 3 compounds inhibited the EGF or PMA-induced production of MUC5AC mucin in NCI-H292 cells. Conclusion: These results indicated that lupenone, lupeol and taraxerol derived from Adenophora triphylla var. japonica regulates the production and gene expression of mucin, by directly acting on airway epithelial cells. In addition, the results partly explain the mechanism of of Adenophora triphylla var. japonica as a traditional remedy for diverse inflammatory pulmonary diseases.

• 환경위생공학
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• 제21권2호
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• pp.1-10
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• 2006

Codonopsis pilosula and Adenophora triphylla were cultivated by sawing seeds, but the germination rate were low. To cultivate these two wild plants was very difficult. The marketable roots of the plants had low quality. The plant pathogens were very difficulty to prevent the diseases (Rhizoctonia, Pythium, Fusarium, Erwinia, Botrytis, Phythophthora) appeared in cultural state. For the extermination of the disease needs a lot of agricultural chemicals, the effect of remain behind an insecticides was high of a hazardous rate after harvest. On this studies, for the safe prevention of the diseases and the promote of seeds germination, we used Bacillus subtilis, B. liquefaciens, Paenibacillus polymixa, Pseudomonas putida separated in our research, these results would bring us harmless products of medicinal herbs for human.

• Plant Resources
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• 제6권2호
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• pp.94-97
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• 2003

The objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on the germination and seedling growth of Adenophora triphylla var. japonica, Codonopsis lanceolata, Platycodon glandiflorum, Which belongs to Campanulaceae and useful for on oriental medicine materials. Seed shape of Codonopsis lanceolata is Elliptic. Platycodon glandiflorum is Oblong. Color of seed coats varied from yellow to dark brown. Seed size of Adenophora triphylla var. japonica is 1.37mm in length, 0.69mm in width. One thousand-seed weight of Adenophora triphylla var. japonica is 258mg. Seed size of Codonopsis lanceolata is 4.45mm in length, 2.11mm in width. One thousand-seed weight of Codonopsis lanoceolata is 1,278mg. Seed size of Platycodon glandiflorum is 2.31mm in length, 1.10mm in width. One thousand-seed weight of Platycodon glandiflorum is 988mg. Optimum temperatures for seed germination and seedling growth range from 20 to $25^{\circ}C$.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.453-454
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• 2010

• 한국약용작물학회지
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• 제28권2호
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• pp.128-135
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• 2020

Background: Sowing seeds of Adenophora triphylla is known to be difficult owing to their small size and irregular seed shape. Therefore, this study was conducted to develop a seed pelleting technique to save labor during sowing. Methods and Results: To identify the optimal germination temperature for A. triphylla seeds, the temperature range was set from 17℃ to 32℃. Germination surveys were conducted in plastic greenhouse conditions in March, April, and May to determine the appropriate sowing time. The optimal germination temperature for A. triphylla seeds was 29℃ and May was the optimal sowing time in plastic greenhouse conditions. Covering materials for seed pelleting used talc (T), kaolin (K), calcium carbonate (C), and vermiculite (V). The pellet binder used agar (A), pectin, xanthan gum, polyvinyl alcohol (PVA), and sodium alginate (S). The best suited treatment mixture were the best suited in kaolin / calcium carbonate / vermiculite (KCV), talc / calcium carbonate / vermiculite (TCV) mixture treatment for covering material, and sodium alginate (S), agar (A) as pellet binder, respectively. The germination rate was the best in TCV mixed with S. Conclusion: The mixture of TCV (2 : 1 : 3) + 1.5% S (TCVS), was found to be the best pelleting materials for A. triphylla seeds, and seed pelleting can be labor-saving during sowing.

• 동아시아식생활학회지
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• 제25권5호
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• pp.813-821
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• 2015

Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. The purpose of the present study was to evaluate the anti-inflammatory and anti-asthma effects of Adenophora triphylla var. japonica extracts. We investigated the molecular mechanism underlying the effects of 80% ethanol extracts (AE) of A. triphylla on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. AE treatment inhibited pro-inflammatory cytokines such as TNF-${\alpha}$ and IL-6 as well as nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. In particular, NO and pro-inflammatory cytokine production was suppressed more effectively by aerial parts (AE-A) than roots (AE-R) of A. triphylla. Quantitative RT-PCR assay showed that AE reduced mRNA levels of iNOS and COX-2. We also evaluated the anti-asthmatic effects of AE-A in an ovalbumin (OVA)-induced BALB/c mouse model. AE-A supplementation significantly reduced the amounts of airway eosinophils, IL-4 and IL-13 levels in BALF, and IgE levels in serum as compared with untreated, OVA-induced mice. These results suggest that AE-A can be considered as a therapeutic agent to potentially relieve asthma.