• 한국전산구조공학회논문집
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• 제17권3호
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• pp.279-293
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• 2004

등방성 혹은 비등방성 적층복합판 및 쉘의 선형 정적 문제와 자유진동 해석이 새로운 변형률 변위 관계가 도입된 개선된 9절점 쉘 요소에 의하여 수행되었다. 그 관계에서 새롭게 추가된 휨 변형률과 변위사이의 관계 항들에 의한 효과는 비틀어진 보 문제에서 검토되었다. 정식화의 전 과정을 통해, 식들의 모든 항들은 자연 좌표계에 기초하고 있다. 가정 자연 변형률 방법이 막 잠김과 전단 잠김 거동을 제거하기 위하여 사용하였다. 적층 복합판 및 쉘의 고유치의 계산을 위해 Lanczos방법을 사용하였고 질량행렬을 구성하기 위하여 Gauss적분법을 사용하였다. 정식화의 유효성을 평가하기 위해 수치 예제를 해석적 해와 비교하였으며, 제시된 결과는 자유진동 조건하에서 적층체의 거동을 이해하는데 유용할 것이다.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.233-240
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• 2016

FK506, a widely used immunosuppressant, is a 23-membered polyketide macrolide that is produced by several Streptomyces species. FK506 high-yielding strain Streptomyces sp. RM7011 was developed from the discovered Streptomyces sp. KCCM 11116P by random mutagenesis in our previous study. The results of transcript expression analysis showed that the transcription levels of tcsA, B, C, and D were increased in Streptomyces sp. RM7011 by 2.1-, 3.1-, 3.3-, and 4.1-fold, respectively, compared with Streptomyces sp. KCCM 11116P. The overexpression of tcsABCD g enes in Streptomyces sp. RM7011 gave rise to approximately 2.5-fold (238.1 μg/ml) increase in the level of FK506 production compared with that of Streptomyces sp. RM7011. When vinyl pentanoate was added into the culture broth of Streptomyces sp. RM7011, the level of FK506 production was approximately 2.2-fold (207.7 μg/ml) higher than that of the unsupplemented fermentation. Furthermore, supplementing the culture broth of Streptomyces sp. RM7011 expressing tcsABCD genes with vinyl pentanoate resulted in an additional 1.7-fold improvement in the FK506 titer (498.1 μg/ml) compared with that observed under non-supplemented condition. Overall, the level of FK506 production was increased approximately 5.2-fold by engineering the supply of allylmalonyl-CoA in the high-yielding strain Streptomyces sp. RM7011, using a combination of overexpressing tcsABCD genes and adding vinyl pentanoate, as compared with Streptomyces sp. RM7011 (95.3 μg/ml). Moreover, among the three precursors analyzed, pentanoate was the most effective precursor, supporting the highest titer of FK506 in the FK506 high-yielding strain Streptomyces sp. RM7011.

• Journal of Ecology and Environment
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• 제47권3호
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• pp.118-133
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• 2023

Background: Free-living amoebae (FLA) are widely distributed in freshwater, seawater, soil, and extreme environments, and play a critical role as feeders on diverse preys in the ecosystem. Also, some FLA can become opportunistic pathogens in animals including humans. The taxa Amoebozoa and Heterolobosea are important amoeboid groups associated with human pathogens. However, the identification and habitat of amoebae belonging to Amoebozoa and Heterolobosea remain poorly reported in the Republic of Korea. This study highlights the first record for identification and source of four amoebae including putative pathogens in the Republic of Korea. Results: In the present study, four previously reported FLA were isolated from freshwaters in Sangju Gonggeomji Reservoir (strain GO001), one of the largest reservoirs during the Joseon Dynasty period, and along the Nakdong River, the largest river in the Republic of Korea (strains NR5-2, NR12-1, and NR14-1) for the first time. Microscopic observations and 18S rDNA phylogenetic trees revealed the four isolated strains to be Acanthamoeba polyphaga (strains NR5-2 and NR12-1), Tetramitus waccamawensis (strain GO001), and Naegleria australiensis (strain NR14-1). Strains NR5-2 and NR12-1 might be the same species and belonged to the morphological Group 2 and the T4 genotype of Acanthamoeba. Strain GO001 formed a clade with T. waccamawensis in 18S rDNA phylogeny, and showed morphological characteristics similar to previously recorded strains, although the species' flagellate form was not observed. Strain NR14-1 had the typical morphology of Naegleria and formed a strongly supported clade with previously recorded strains of N. australiensis in phylogenetic analysis of 18S rDNA sequences. Conclusions: On the bases of morphological and molecular analyses, four strains of FLA were newly observed and classified in the Republic of Korea. Three strains belonging to the two species (A. polyphaga and N. australiensis) isolated from the Nakdong River have the potential to act as opportunistic pathogens that can cause fatal diseases (i.e. granulomatous amoebic encephalitis, Acanthamoeba Keratitis, and meningoencephalitis) in animals including humans. The Nakdong River in the Republic of Korea may provide a habitat for potentially pathogenic amoebae, but additional research is required to confirm the true pathogenicity of these FLA now known in the Republic of Korea.

• 콘크리트학회논문집
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• 제26권3호
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• pp.247-254
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• 2014

화재시 콘크리트 구조물은 구성재료의 상이한 열적특성으로 인해 강도가 저하하고 동시에 수직부재는 수평부재의 팽창에 의한 모멘트하중을 받아 전단파괴가 발생한다. 따라서 여러가지 화재곡선을 사용한 콘크리트 구조물의 화재시 거동에 대한 연구가 많이 이루어졌지만 주로 온도상승구간에서 발생하는 폭렬특성과 열팽창변형에 관한 연구가 대부분이다. 하지만 고온이 유지될 경우 발생할 수 있는 크리프변형은 화재시 구조물의 안정성에 큰 영향을 미치지만 상대적으로 연구가 미진한 상태이다. 또한 이러한 고온을 받는 콘크리트의 안정성에는 체적의 대부분을 차지하는 굵은골재의 열적특성이 큰 영향을 미치기 때문에 이 연구에서는 화강암계, clay계, clay-ash계 세 종류의 굵은골재를 사용한 콘크리트의 고온 역학적 특성을 평가했다. 그 결과 굵은골재의 성인으로 인한 내부공극 때문에 경량골재를 사용한 콘크리트가 일반골재를 사용한 콘크리트보다 높은 고온강도 및 탄성계수를 나타냈고 열팽창변형과 전체변형의 경우 더 낮은 변형률을 나타내어 온도상승구간에서의 구조적 안정성 측면에서 유리한 것으로 판단되었다. 그러나 고온크리프의 경우 내부공극으로 인해 더 큰 수축량이 발생하기 때문에 내화성능설계시에 이에 대한 추가적인 고려가 필요할 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제27권9호
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• pp.1701-1710
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• 2017

Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that $RecCpp40_{+10}$ failed to induce fever in rabbits, whereas $RecCpp80_{+10}$ caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.

• Journal of Cardiovascular Imaging
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• 제30권3호
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• pp.185-196
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• 2022

BACKGROUND: Two-dimensional (2D) strain provides more predictive power than ejection fraction (EF) in patients with ST-elevation myocardial infarction (STEMI). 3D strain and EF are also expected to have better clinical usefulness and overcome several inherent limitations of 2D strain. We aimed to clarify the prognostic significance of 3D strain analysis in patients with STEMI. METHODS: Patients who underwent successful revascularization for STEMI were retrospectively recruited. In addition to conventional parameters, 3D EF, global longitudinal strain (GLS), global area strain (GAS), as well as 2D GLS were obtained. We constructed a composite outcome consisting of all-cause death or re-hospitalization for acute heart failure or ventricular arrhythmia. RESULTS: Of 632 STEMI patients, 545 patients (86.2%) had a reliable 3D strain analysis. During median follow-up of 49.5 months, 55 (10.1%) patients experienced the adverse outcome. Left ventricle EF, 2D GLS, 3D EF, 3D GLS, and 3D GAS were significantly associated with poor outcomes. (all, p < 0.001) The maximum likelihood-ratio test was performed to evaluate the additional prognostic value of 2D GLS or 3D GLS over the prognostic model consisting of clinical characteristics and EF, and the likelihood ratio was 15.9 for 2D GLS (p < 0.001) and 1.49 for 3D GLS (p = 0.22). CONCLUSIONS: The predictive power of 3D strain was slightly lower than the 2D strain. Although we can obtain 3D strains, volume, and EF simultaneously in same cycle, the clinical implications of 3D strains in STEMI need to be investigated further.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.157-157
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• 2010

Vertical-structure light-emitting diodes (V-LEDs) by laser lift-off (LLO) have been exploited for high-efficiency GaN-based LEDs of solid-state lightings. In V-LEDs, emitted light from active regions is reflected-up from reflective ohmic contacts on p-GaN. Therefore, silver (Ag) is very suitable for reflective contacts due to its high reflectance (>95%) and surface plasmon coupling to visible light emissions. In addition, low contact resistivity has been obtained from Ag-based ohmic contacts annealed in oxygen ambient. However, annealing in oxygen ambient causes Ag to be oxidized and/or agglomerated, leading to degradation in both electrical and optical properties. Therefore, preventing Ag from oxidation and/or agglomeration is a key aspect for high-performance V-LEDs. In this work, we demonstrate the enhanced thermal stability of Ag-based Ohmic contact to p-GaN by reducing the thermal compressive stress. The thermal compressive stress due to the large difference in CTE between GaN ($5.6{\times}10^{-6}/^{\circ}C$) and Ag ($18.9{\times}10^{-6}/^{\circ}C$) accelerate the diffusion of Ag atoms, leading to Ag agglomeration. Therefore, by increasing the additional residual tensile stress in Ag film, the thermal compressive stress could be reduced, resulting in the enhancement of Ag agglomeration resistance. We employ the thin Ni layer in Ag film to form Ni/Ag mutli-layer structure, because the lattice constant of NiO ($4.176\;{\AA}$ is larger than that of Ag ($4.086\;{\AA}$). High-resolution symmetric and asymmetric X-ray diffraction was used to measure the in-plane strain of Ag films. Due to the expansion of lattice constant by oxidation of Ni into NiO layer, Ag layer in Ni/Ag multi-layer structure was tensilely strained after annealing. Based on experimental results, it could be concluded that the reduction of thermal compressive stress by additional tensile stress in Ag film plays a critical role to enhance the thermal stability of Ag-based Ohmic contact to p-GaN.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.27-36
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• 2022

Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.

• 콘크리트학회논문집
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• 제27권5호
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• pp.489-499
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• 2015

내진 보강 공법의 보강효과 파악을 위해선 이에 대한 이론적 규명이 필요하다. 이에 본 연구는 단부에 기둥 부재를 증타한 단근 배근된 전단벽체를 대상으로 해 보강되는 기둥의 상세와 일체화 거동을 고려한 전단강도 모델을 제안했다. 이 모델은 증타된 기둥부재의 전단변형이 집중되는 길이를 가정해 이를 일체화된 벽체 내에 발생하는 스트럿 두께 산정에 이용했다. 뿐만 아니라 이 길이 내에 집중 발생하는 전단변형률을 유도, 이를 일체화 거동을 고려한 적합성 조건을 기존 전단벽체의 해석 알고리즘에 도입함으로써 증타 보강된 벽체의 해석 알고리즘을 새로이 제안했다. 또한 제안된 알고리즘을 통해 계산된 전단강도로 횡력 저항 메커니즘을 단일 스트럿 화 시켜줌으로써 보강부재의 초기강성을 제안하였다. 본 연구에서 제안한 방법의 타당성을 확인하기 위해 해석 결과 값을 본 연구에서 수행한 증타 보강된 벽체를 대상으로 한 실험뿐만 아니라 기존 RC 골조 내에 RC 전단벽체를 신설해 보강한 실험의 결과와 비교해 본 결과, 기둥부재의 상세를 고려하면서 동시에 기존 기준들에 비해 실험결과에 가깝게 예측할 수 있음을 확인했다.

• 한국지반공학회논문집
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• 제24권12호
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• pp.113-120
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• 2008

1차원 지반응답해석은 지반에 의한 지진동의 증폭현상을 모사하는 데 널리 사용되고 있다. 가장 널리 사용되고 있는 등가선형 지반응답해석기법은 유효변형률에 상응하는 전단탄성계수와 감쇠비를 전 주파수 영역에 일률적으로 적용하며, 진동주파수에 대한 지반 응답의 영향을 고려하지 않는다. 지반의 비선형 거동을 주파수영역에서 보다 정확하게 모사하기 위하여 진동주파수와 지반거동의 상관관계를 주파수-변형률 곡선으로써 모의하는 등가선형해석기법이 개발되었으며, 이 방법은 기존의 등가선형해석기법에 비하여 정확성을 향상시킨다고 알려져 있다. 이제까지 다양한 형상의 주파수변형률 곡선이 제안되었으며, 이들은 한결같이 해석의 정확성을 증가시킨다고 주장된 바 있다. 본 연구에서는 기존의 연구에서 제안되었건 두 개의 주파수-변형률 관계 완화곡선과 본 연구에서 새롭게 제안된 세 개의 곡선을 이용하여 주파수 의존성을 고려한 등가선형해석기법의 정확성을 검증하였다. 검증에는 세 개의 부지에서 기록된 6개의 지진파가 사용되었다. 해석결과, 완화곡선은 지반응답에 지배적인 영향을 미치는 것으로 나타났으며, 주파수 의존성을 고려한 해석은 정확성을 향상시킬수 있는 것으로 나타났다. 하지만, 모든 사례에서 가장 정확하게 응답을 예측하는 완화곡선은 없는 것으로 나타났으며, 해석사례별로 최적의 완화곡선이 다른 것으로 나타났다. 따라서, FDEL 해석 수행 시, 다양한 완화곡선을 사용해야 할 것으로 판단되며 등가선형해석과 병행해서 수행되어야 한다고 판단된다.