• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1212-1220
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• 2013

Because the cholesterol oxidase from Brevibacterium sp. M201008 was not as stable as the free enzyme form, it had been covalently immobilized onto chemically modified Sepharose particles via N-ethyl-N'-3-dimethylaminopropyl carbodiimide. The optimum immobilization conditions were determined, and the immobilized enzyme activity obtained was 12.01 U/g Sepharose-ethylenediamine. The immobilization of the enzyme was characterized by Fourier transform infrared spectroscopy. The immobilized enzyme exhibited the maximal activity at $35^{\circ}C$ and pH 7.5, which was unchanged compared with the free form. After being repeatedly used 20 times, the immobilized enzyme retained more than 40.43% of its original activity. The immobilized enzyme showed better operational stability, including wider thermal and pH ranges, and retained 62.87% activity after 20 days of storage at $4^{\circ}C$, which was longer than the free enzyme.

• The Journal of Korean Academic Society of Nursing Education
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• v.9 no.2
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• pp.276-287
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• 2003

The purpose of this study is to examine the impact of social voluntary activity on the identity of women's college students. universities are functionally constituted by teaching, researching and social service. Data for analysis are collected 439 students at K women's college in other to examine research questions, t-test and regression test. The results of this analysis are as follows : First, There was any gap in self-esteem, morality and social welfare ideology between the college students who had ever experienced social voluntary activity and who had not. Second, The personal characteristics did not give an impact, but their satisfaction was under the influence of institutional support for supervision and altitude of themselves for social service, which were part of the institution characteristics. Third, The social service effectiveness was not affected by the personal characteristics, except influenced by the instituitional characteristits such as degree of compensation an influence on self-esteem. also social welfare and morality were not affected. Fourth, There was any gap in self-esteem, morality and social welfare ideology between the impact of service satisfaction on service effectiveness.

• Korean Journal of Pharmacognosy
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• v.43 no.3
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• pp.239-242
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• 2012

This study was carried out to investigate the anti-malarial activity of Juniperus chinensis by in vitro and in vivo system using Plasmodium falciparum chloroquine-sensitive(3D7) and P. falciparum chloroquine-resistant(S20) strains. According to cytotoxicty test on NIH 3T3 cell, the ethanol extract(EtOH), ethylacetate(EtOAc) fraction and aqueous fraction possessed significant anti-malarial activity against both 3D7 and S20 strains at non-toxic concentrations(<100 /). In vitro assay, EtOAc fraction showed notable activity against 3D7 and S20 strains of P. falciparum with $IC_{50}$ values of $37{\pm}2{\mu}g/ml$ and $36{\pm}6{\mu}g/ml$. In animal test using P. falciparum infected human erythrocytes, the treatment of EtOAc fraction significantly inhibited parasitaemia in mice in a dose-dependent manner that is parasitaemia of 42%, 34% and 31% in doses of 10 mg/kg, 20 mg/kg and 40 mg/kg, respectively. The study provides data to support the medicinal importance of the J. chinensis.

• Journal of the Korean Wood Science and Technology
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• v.29 no.3
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• pp.104-111
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• 2001

There are some evidence that active enzymatic proteins, e.g. fungal laccase, exist in the naturally occured soil humus. This study was performed to investigate the covalent binding of fungal laccase to the humic acid-iron complex, and to measure laccase activity of immobilized ones. Seven methods were adopted to form the covalent binding of fungal laccase with soil humic acids complexed with iron. Using these seven methods it was possible to change the dimension of spacer arm between laccase and support, and also to regulate the mode of covalent binding of this enzyme. The spacer arm was regulated from 2C to 11C. There was not observed any straight relationship between the spacer arm longitude and the laccase activity after immobilization, but the binding mode more effective than the former. Three out of the seven methods gave the high activity of immobilized laccase, and which active products of laccase immobilization was stable up to 10 days after the process. It is indicated that natural soil condition might be prevented the laccase activation by the toxic influence of some phenolic humic compounds. It was shown, for the first time, the possibilities to obtain the high activity of fungal laccase by binding to humic acids, and especially in complex with iron.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.8 no.3
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• pp.145-150
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• 2014

In this paper, we propose a novel scheme to improve the performance of a voice activity detection(VAD) which is based on the deep belief networks(DBN) with the likelihood ratio(LR). The proposed algorithm applies the DBN learning method which is trained in order to minimize the probability of detection error instead of the conventional decision rule using geometric mean. Experimental results show that the proposed algorithm yields better results compared to the conventional VAD algorithm in various noise environments.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.2
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• pp.473-488
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• 2016

The purpose of this study is to present as an alternative to a new policy of the regional level about actively adopting of the Sharing Culture and to suggest policy direction of Talent Sharing activities as an intermediary Sharing activities. To achieve this, We derive a significant outcomes as compared mutual to the center of a volunteer manager and volunteer groups for talent sharing activities in Busan Area. As a result, several suggestion are presented as follow : First, we needed to build for systematic control of talent sharing activities around existing volunteer management institution. Second, Supply and Demand control system is needed to build for talent sharing activities at the local government dimension. Third, To reflect the trend of the people watching the activity with some degree of talent, expertise as a talent sharing activity, Talent sharing volunteers are needed for specialized capability verification. Finally, Sharing activities are needed to settle major policy for the systematic management of talent sharing activity and to expanded budget support of local government at the local government dimension.

• Journal of International Academy of Physical Therapy Research
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• v.9 no.4
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• pp.1576-1585
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• 2018

Regularly participating in physical activity is known to improve quality of life and body composition in elderly with stroke. However, comparatively less physical activity is performed by the stroke survivors. The factors related to inactivity in elderly female stroke survivors have not been elucidated. Therefore, this study aims to compare the quality of life factors and limb compositions between the active and inactive elderly female stroke survivors. Forty nine subjects between the ages of 65 to 75 years were selected from the KNHANES data between the years 2009 to 2011. In addition, 186 agematched healthy peers were also selected for limb composition comparisons. The subjects were groups based on walking days per week: walkers; 3 days or more, non-walkers; less than 3 days per week. BMI and waist circumference were within the obesity ranges for both the non-walkers and walkers. As results, the trend for greater fat (${\pm}10%$) and lean mass (${\pm}30%$) differences were observed for non-walker and walkers, respectively. Significantly greater reasons for function limitation by stroke and hypertension were reported with significantly greater self-care difficulty was shown by the walkers. In conclusion, elderly female stroke survivals may require customized motivation and continuous support to participate in physical activity regularly.

• Journal of Fisheries and Marine Sciences Education
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• v.16 no.2
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• pp.171-180
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• 2004

The purpose of the present study is to study how the understanding of growing of child to be related with recognizing the role of parents and activity of bringing up, and at the same time verifying a part of father through reviewing difference view with mother's. Object of investigation were 396 members of parents who have babies in infancy (177 members of fathers and 219 members of mothers. ). The result of this research shows that father has stronger confidence than mother in the role of parents relatively, but he does not understand as much as mother does in growing of child, and is not active enough in bringing up, keeps less intimate relationship with his child and does not affect on his child than mother does. And father who has more understanding on growing of child has also more positive activity in bringing up child. We can get one conclusion from this research, now a days it is seriously required for a father to participate more corporately in bringing up his child, we should do support more effort to let father gets better and right understanding in growing of child and building-up more effective and positive relation with his child.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.835-845
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• 2018

We studied the role of ethylene control in regulating energy metabolism, antioxidant enzyme activity, and vase life of cut rose Rosa hybrida 'Carola'. Rose flowers at stage II were sprayed with one of the following solutions: water (control), $10{\mu}L\;L^{-1}$ 1-methylcyclopropene (1-MCP), or $0.5g\;L^{-1}$ 2-chloroethanephosphonic acid (ethephon). After harvest, ethylene production rate, respiration intensity, energy charge (EC), activities of energy metabolism-related and antioxidant enzymes, and malondialdehyde (MDA) content were measured. Results showed that 1-MCP enhanced the activities of superoxide dismutase, $H^+$-adenosine triphosphatase, $Ca^{2+}$-adenosine triphosphatase, succinic dehydrogenase, and cytochrome c oxidase, increased adenosine triphosphate (ATP) content, maintained high EC levels, inhibited respiration intensity, reduced peroxidase (POD) and polyphenol oxidase (PPO) activity and MDA accumulation, and prolonged vase life. Ethephon promoted ethylene production and respiration intensity, increased POD and PPO activity, reduced ATP content and EC levels, and accelerated senescence. Our results support a novel role for ethylene control in regulating senescence of 'Carola'.

• International conference on construction engineering and project management
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• 2017.10a
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• pp.80-88
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• 2017

Although hazard identification is one of the most important steps of safety management process, numerous hazards remain unidentified in the construction workplace due to the dynamic environment of the construction site and the lack of available resource for visual inspection. To this end, our previous study proposed the collective sensing approach for safety hazard identification and showed the feasibility of identifying hazards by capturing collective abnormalities in workers' walking patterns. However, workers generally performed different activities during the construction task in the workplace. Thereby, an additional process that can identify the worker's walking activity is necessary to utilize the proposed hazard identification approach in real world settings. In this context, this study investigated the feasibility of identifying walking activities during construction task using Wearable Inertial Measurement Units (WIMU) attached to the worker's ankle. This study simulated the indoor masonry work for data collection and investigated the classification performance with three different machine learning algorithms (i.e., Decision Tree, Neural Network, and Support Vector Machine). The analysis results showed the feasibility of identifying worker's activities including walking activity using an ankle-attached WIMU. Moreover, the finding of this study will help to enhance the performance of activity recognition and hazard identification in construction.