Browse > Article

In vitro Anti-malarial Activity of Juniperus Chinensis Extract  

Lee, Keyong-Ho (Korea Prime Pharm.)
Kim, Byeong-Soo (College of Industrial Sciences, Kongju National University)
Ho, Choe-Yeong (College of Industrial Sciences, Kongju National University)
Rhee, Ki-Hyeong (College of Industrial Sciences, Kongju National University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.3, 2012 , pp. 239-242 More about this Journal
Abstract
This study was carried out to investigate the anti-malarial activity of Juniperus chinensis by in vitro and in vivo system using Plasmodium falciparum chloroquine-sensitive(3D7) and P. falciparum chloroquine-resistant(S20) strains. According to cytotoxicty test on NIH 3T3 cell, the ethanol extract(EtOH), ethylacetate(EtOAc) fraction and aqueous fraction possessed significant anti-malarial activity against both 3D7 and S20 strains at non-toxic concentrations(<100 /). In vitro assay, EtOAc fraction showed notable activity against 3D7 and S20 strains of P. falciparum with $IC_{50}$ values of $37{\pm}2{\mu}g/ml$ and $36{\pm}6{\mu}g/ml$. In animal test using P. falciparum infected human erythrocytes, the treatment of EtOAc fraction significantly inhibited parasitaemia in mice in a dose-dependent manner that is parasitaemia of 42%, 34% and 31% in doses of 10 mg/kg, 20 mg/kg and 40 mg/kg, respectively. The study provides data to support the medicinal importance of the J. chinensis.
Keywords
Juniperus chinensis; Malaria; Plasmodium falciparum;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Breman, J. G. (2001) The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden. Am. J. Trop. Med. Hyg. 64(1-2 Suppl): 1-11.
2 Mendis, K., Sina, B., Marchesini, P. and Carter, R. (2001) The reglected burden of Plasmodium vivax malaria. Am. J. Trop. Med. Hyg. 64: 97-106.
3 Kelly, J. X., Smilkstein, M. J., Cooper, R. A., Lane, K. D., Johnson, R. A., Janowsky, A., Dodean, R. A., Hinrichs, D. J., Winter, R. and Riscoe, M. (2007) Design, synthesis, and evaluation of 10-N-substituted acridones as novel chemosensitizers in Plasmodium falciparum. Antimicrob. Agents Chemother. 51(11): 4133-4140   DOI   ScienceOn
4 Gessler, M. C., Nkunya, M. H. H., Mwasumbi, L. B., Henrich, M. and Tanner, M. (1993) Screening Tanzanian plants for antimalarial activity. Acta Trop. 56: 65-77.
5 Tran, Q. L., Tezuka, Y., Ueda, J. Y., Nguyen, N. T., Maruyama, Y., Begum, K., Kim, H. S., Wataya, Y., Tran, Q. K. and Kadota, S. (2003) In vitro antiplasmodial activity of antimalarial medicinal plants used in Vietnamese traditional medicine. J. Ethnopharmacol. 86(2-3): 249-252.   DOI   ScienceOn
6 Bae, Y. S., Si C. L., Kim, J. K. and Darchesy, J. J. (2005) Extractive of Juniper needle. J. Kor. For. En. 24: 24-30.
7 Kim, S. J., Jung, J. Y., Kim, H. W. and Park, T. (2008) Antiobesity effects of Juniperus chinensis extract are associated with increased AMP-activated protein kinase expression and phosphorylation in the visceral adipose tissue of rats. Biol. Pharm. Bull. 31:1415-1421.   DOI   ScienceOn
8 Lim, J. P., Song, Y. C., Kim, J. W., Ku, C. H., Eun, J. S., Lee, K. H. and Kim, D. K. (2002) Free radical scavengers from the heartwood of Juniperus chinensis. Arch. Pharm. Res. 25:449-452.   DOI   ScienceOn
9 Lee, C. H., Park, J. M., Song, H. Y., Jeong, E. Y. and Lee, H. S. (2009) Acaricidal activities of major constituents of essential oil of Juniperus chinensis leaves against house dust and stored food mites. J. Food Prot. 72(8): 1686-1691.
10 Satayavivad, J., Watcharasit, P., Khamkong, P., Tuntawiroon, J., Pavaro, C. and Ruchirawat, S. (2004). The pharmacodynamic study of a potent new antimalarial (MC1). Acta Trop. 89: 343-349.   DOI   ScienceOn
11 Mosmann, T. (1983) Rapid colorimetric assays for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63.   DOI   ScienceOn
12 Walliker, D., Quakyi, I. A., Wellems, T. E., McCutchan, T. F., Szarfman, A., London, W. T., Corcoran, L. M., Burkot, T. R. and Carter, R. (1987) Genetic analysis of the human malaria parasite Plasmodium falciparum. Science 236: 1661-1666.   DOI
13 Di Santi, S. M., Boulos, M., Vasconcelos, M. A., Oliveira, S., Couto, A. and Rosario, V. E. (1987) Characterization of Plasmodium falciparum strains of the State of Rondonia, Brazil, using microtests of sensitivity to antimalarials, enzyme typing and monoclonal antibodies. Rev. Inst. Med. Trop. Sao Paulo 29: 142-147.   DOI   ScienceOn
14 Trager, W. and Jensen, J. B. (1976) Human malaria parasites in continuous culture. Science 193: 673-675.   DOI
15 Prachayasittikul, S., Saraban1, P., Cherdtrakulkiat, R., Ruchirawat, S. and Prachayasittikul, V. (2010) New bioactive triterpenoids and antimalarial activity of Diospyros rubra Lec. EXCLI J. 9:1-10
16 Soh, P. N., Witkowski, B., Olagnier, D., Nicolau, M. L., Garcia-Alvarez, M. C., Berry, A. and Benoit-Vical, F. (2009) In vitro and in vivo properties of ellagic acid in malaria treatment. Antimicrob. Agents Chemother. 53: 1100-1106.   DOI   ScienceOn
17 Kayano, A. C., Lopes, S. C., Bueno, F. G., Cabral, E. C., Souza-Neiras, W. C., Yamauchi, L. M., Foglio, M. A., Eberlin, M. N., Mello, J. C. and Costa, F. T. (2011) In vitro and in vivo assessment of the anti-malarial activity of Caesalpinia pluviosa. Malar. J. 10: 112-116.   DOI   ScienceOn
18 Chung, I. M., Kim, M. Y., Park, S. D., Park, W. H. and Moon, H. I. (2009) In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum. Phytother. Res. 23(11): 1634-1637.   DOI   ScienceOn
19 Kihampa, C., Nkunya, M. H., Joseph, C. C. and Magesa, S. M. (2010) Antimosquito phenylpropenoids from the stem and root barks of Uvariodendron pycnophyllum (Diels) R.E.Fr. J. Appl. Sci. Environ. Manage. 14(3): 29-32.
20 Deharo, E., Bourdy, G., Munoz, V., Ruiz, G. and Sauvain, M. (2001) A search for natural bioactive compounds in Bolivia through a multidisciplinary approach. Part V. Evaluation of the antimalarial activity of plants used by the Tacana indians. J. Ethnopharm. 77: 91-98.   DOI   ScienceOn
21 Liu, A. L., Shu, S. H., Qin, H. L., Lee, S. M., Wang, Y. T. and Du, G. H. (2009) In vitro anti-influenza viral activities of constituents from Caesalpinia sappan. Planta Med. 75: 337-339.   DOI   ScienceOn
22 Pereira, M. V., Dias, C. S., Costa, V. O., Conde, N. O. and Buzalaf, M. R. (2009) In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens. J. Ethnopharm. 124: 289-294.   DOI   ScienceOn
23 Rao, Y. K., Fang, S. H. and Tzeng, Y. M. (2005) Anti-inflammatory activities of flavonoids isolated from Caesalpinia pulcherrima. J. Ethnopharm. 100: 249-253.   DOI   ScienceOn
24 Shukla, S., Mehta, A., John, J., Singh, S., Mehta, P., Vyas, S. P. (2009) Antioxidant acitivity and total phenolic content of ethanolic extract of Caesalpinia bonducella seeds. Food Chem. Toxicol. 47: 1848-1851.   DOI   ScienceOn
25 Bero, J., Frederich, M. and Quentin-Leclercq, J. (2009) Antimalarial compounds isolated from plants used in traditional medicine. J. Pharm. Pharmacol. 61: 1401-1433.   DOI   ScienceOn