• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4357-4361
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• 2015

Thunbergia Laurifolia Linn. (TL) is one of the most familiar plants in Thai traditional medicine that is used to treat various conditions, including cancer. However, the antitumor activity of TL or its constituents has never been reported at the molecular level to support the folklore claim. The present study was designed to investigate the antitumor effect of an aqueous extract of TL in human breast cancer cells and the possible mechanism(s) of action. An aqueous crude extract was prepared from dried leaves of TL. Folin-Ciocalteu colorimetric assays were used to determine the total phenolic content. Antiproliferative and cell cycle effects were evaluated in human breast adenocarcinoma MCF-7 cells by MTT reduction assay, cell growth inhibition, clonogenic cell survival, and flow cytometric analysis. Free radical generation by the extracts was detected using electron paramagnetic resonance spectroscopy. The exposure of human breast adenocarcinoma MCF-7 cells to a TL aqueous extract resulted in decreases in cell growth, clonogenic cell survival, and cell viability in a concentration-dependent manner with an $IC_{50}$ value of $843{\mu}g/ml$. Treatments with extract for 24h at $250{\mu}g/ml$ or higher induced cell cycle arrest as indicated by a significant increase of cell population in the G1 phase and a significant decrease in the S phase of the cell cycle. The capability of the aqueous extract to generate radical intermediates was observed at both high pH and near-neutral pH conditions. The findings suggest the antitumor bioactivities of TL against selected breast cancer cells may be due to induction of a G1 cell cycle arrest. Cytotoxicity and cell cycle perturbation that are associated with a high concentration of the extract could be in part explained by the total phenolic contents in the extract and the capacity to generate radical intermediates to modulate cellular proliferative signals.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.757-763
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• 1999

The metal specificity of D-xylose isomerase from Streptomyces rubiginosus was examined by site-directed mutagenesis. The activation constants for metal ion ($Mg^{2+},{\;}Mn^{2+},{\;}or{\;}Co^{2+}$) of wild-type and mutant enzymes were determined by titrating the metal ion-free enzyme with $Mg^{2+},{\;}Mn^{2+},{\;}and{\;}Co^{2+}$, respectively. Substitutions of amino acids either on coordinated or around the M2 site (His-22O, Asn-185, Glu-186, and Glu-221) dramatically affected the activation constants as well as activity. A decrease of metal binding affinity was most significant in the presence of $Mg^{2+}$. When compared with the wild-type enzymes, the binding affinity of H220S and Nl85K for Mg^{2+} was decreased by 10-15-fold, while the affinity for $Mn^{2+}{\;}or{\;}Co^{2+}$ only decreased by 3-5-fold. All the mutations close to the M2 site changed their metal preference from $Mg^{2+}{\;}to{\;}Mn^{2+}{\;}or{\;}Co^{2+}$. These altered metal preferences may be caused by a relatively weak binding affinity of $Mg^{2+}$ to the enzyme. Thermal inactivation studies of mutants at the M2 site also support the importance of the M2 site geometry for metal specificity as well as the thermostability of the enzyme. Mutations of other important groups hardly affected the metal preference, although pronounced effects on the kinetic parameters were sometimes observed. This study proposes that the metal specificity of D-xylose isomerase can be altered by the perturbation of the M2 site geometry, and that the different metal preference of Group I and GroupII D-xylose isomerases may be caused by nonconserved amino acid residues around the M2 site.

• Korean Journal of Human Ecology
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• v.19 no.5
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• pp.775-794
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• 2010

This study is to provided a basic model to encourage environment and outdoor play programs. The study was based on the analyses of actual activities performed in two child care centers in Daejeon area and the way in which the programs for outdoor play were related to the environment of the centers. Data were collected through 'The Daily Report of the Outdoor Play Programs: Plan and Evaluation' paper conducted at each center performed from 2007 to 2009. Following Mockwha Choi et al., (2007), the play observed and described by teachers was analyzed to classify play areas and play activities within each area. The places, equipment and, playthings using in each play period were also examined as well as the limitation and inspiration caused by the environment. The results of the study are as follows: 1) Empty and unfilled space tended to generate more types of non-structural play and role-pretend play 2)Adventurous play was rarely observed. More thoughtfully designed physical environments need to be provided for children to experience challenge and adventure through physical activities 3) Outdoor play environment should be suitable for the overall developments of infants and toddlers depending on their activities. A child care specialist should support the design such environment 4) Naturally, the programs of outdoor play will not be identical for all child care centers. Rather, they should go along with the programmatic characters and environmental attributes of each center. Therefore the awareness of teachers and parents as to the importance of must realize of outdoor play should be further increased.

• Proceedings of the CALSEC Conference
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• 2002.01a
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• pp.107-131
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• 2002

Recently, it is an important problem that combines part of sale, technical development etc.. as well as function that is the multi-item and small quantity production environment and production to computer network that supports synthetic efficiency of general corporation activity in manufacturing industry. Integration production information system is developed by these requests and globalization of production system and marketing by tough international competition of various industry product need IMS(Intelligent Manufacturing System) that cope in these change. Therefore, this paper searches analysis method, problem and development way for information system of manufacture field to construct this IMS efficiently, and develops each information strategy planning establishment step, product and integral way of products which is the systematic establishment way of essential information strategy planning in efficient manufacturing information system construction. Also, this paper presents detailed system design methods of each step and templates for efficient methodology of information strategy planning to support IMS efficiently.

• The Journal of Society for e-Business Studies
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• v.9 no.2
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• pp.163-181
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• 2004

Recently, it is to increase rapidly in people who do a private business through Web service. So, many companies have been developing part of businesses using Web Services. The greatest interest of companies developing Web Services was that users were easily discovering and using information on theirs. So, companies have been taking interest in a standard that supports registrations of information on Web service and discoveries of a registered Web services. Therefore UDDI 3.0 standard of the registry for Web service standardized by OASIS is in the spotlight. But, because of the complexity of it, there are no implementations developed in the inside of the country yet. And a development activity of it is faint. In outside of the country, IBM, Microsoft and SAP has developed it, but they do not perfectly support a standard of UDDI 3.0. This Paper shows the development of the Registry System which supports perfectly a standard of UDDI 3.0.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.3
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• pp.99-105
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• 2015

Hsp33 is a prokaryotic molecular chaperon that exerts a holdase activity upon response to an oxidative stress at raised temperature. In particular, intramolecular disulfide bond formation between the four conserved cysteines that bind a zinc ion in reduced state is known to be critically associated with the redox sensing. Here we report the backbone NMR assignment results of the half-oxidized Hsp33, where only two of the four cysteines form an intramolecular disulfide bond. Almost all of the resolved peaks could be unambiguously assigned, although the total assignments extent reached just about 50%. Majority of the missing assignments could be attributed to a significant spectral collapse, largely due to the oxidation-induced unfolding of the C-terminal redox-switch domain. These results support two previous suggestions: conformational change in the first oxidation step is localized mainly in the C-terminal zinc-binding domain, and the half-oxidized form would be still inactive. However, some additional regions appeared to be potentially changed from the reduced state, which suggest that the half-oxidized conformation would be an intermediate state that is more labile to heat and/or further oxidation.

• Journal of the Korean Society of Costume
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• v.52 no.1
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• pp.145-158
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• 2002

The so-called 'Bustle'which was a support put on waist to heave the side the buttocks means the grandiloquent style to express to ideal body. Bustle style. one of the outstanding and remarkable fashion trends in the late 20th century, has significant meaning in fashion history. This is because not only the style has the longest history, but also it has been periodically repeated every end of a century from the 17th to the 20th century. To analyse to cause the Bustle style was shown, I studied first the sign of forthcoming activity of the Bustle style, the change of the Bustle style shown In the times at the end of century and feature of the Bustle style. Based on the previous reasons of appearance of the Bustle style In the history. there are two similarities. First. reaction led to an occurrence of the reactionary tendency, the Bustle style as they were eager for the past because of a feeling of uneasiness about a coming century. Second, the principle of retrogression led to an occurrence of the Bustle style craving for new style. This Bustle style welch was reaction from being tired of the former fashion appeared to expression a desire for new style. In short, Bustle style was an intermediation between different histories and the basis for continuous development of new eras. rather than lust a part of classicism.

• Journal of KIISE:Software and Applications
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• v.34 no.8
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• pp.752-762
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• 2007

The PSP (Personal Software Process) is developed to help developers make high-quality products through improving their personal process. With consistent measurement and analysis activity that the PSP suggests, developers can identify process deficiencies and make reliable estimates on effort and quality. However, due to the high-overhead and context-switching problem of manual data recording, developers have difficulties in collecting reliable data, which can lead wrong analysis results. On the other hand, the paper-based process guides of the PSP are inconvenient to navigate its process information and difficult to attach additional information. In this paper, we introduce a PSP supporting tool developed to handle these problems. The tool provides automated data collection facilities to help acquire reliable data, an EPG (Electronic Process Guide) for the PSP to provide easy access and navigation of the process information, and an experience repository to store development experience as additional information about the process.

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.241-253
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• 2011

The present thesis is to concretize process stages and methods for effective use of online CGTS(creative group thinking system), or the web-storming program, in space design and to propose a support system for idea generation specific to space design. For the purpose of this study, activity factors in space design process and functional factors of the web-storming program were written into an interaction matrix, which was used for a questionnaire survey on correlations. Then, the results of the interaction matrix were visualized and analyzed following the procedure of observation-interpretation-application. The analysis found that the schematic design stage is most correlated where close collaboration is performed in the process of space design and that differences in correlation arise upon division of work. As such, the present study proposed 'provision of media for ideas in accordance with specific process and UI optimization' as a means of improving usability in schematic design that is closely correlated and suggested an idea to enhance the applicability of web-storming system in division of work.

• The Korean Journal of Community Living Science
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• v.17 no.1
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• pp.109-122
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• 2006

As we encounter the global and localized era, the development operations on a regional level are in active promotion. This manuscript has been completed with the purpose of probing for course of action in lifelong learning movement in terms of activating and developing of local communities. For this, the comparative analysis of practiced cases in America's community school movement, Japan's movement for establishing lifelong learning village and Sweden's study circle movement have been made. For the analytical frame of the comparison, the actual results on background of promotion, themes for practice, details of practice, methods for practice of local community centered lifelong learning movement have been applied. As a result of analysis, the local community centered lifelong learning movement has been promoted to break each country's social and economic crisis and to activate the local community. The promotion of each operation has been accomplished with the support of specific organization and the participants were the citizens of the local community. Also, the details of practice are composed of operating the people-centered lifelong learning program, cooperative learning by local citizens and local community realization activity. The details of education is closely related with the life of learners. Therefore, the lifelong movement for the activation of local community hereafter should be promoted based on the coherence of local community, should be able to contain the actual life of the citizens and should be practiced as a process of forming the lifelong learning group at concerned local community through a democratic learning process.