• Korean Journal of Food Science and Technology
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• v.33 no.4
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• pp.486-491
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• 2001

The pathogenicity for one hundred strains of domestic and foreign Y. enterocolitica was tested with HEp-2 cell invasion method as a reference. The serotyping, biotyping, PCR and esculin hydrolyis, salicin fermentation, pyrazinamidase activity, indole production, xylose fermentation, CRMOX and autoagglutination were compared to determine the possibility of pathogenic detection method. According to the test results, serotyping was limited to verify pathogenicity, however, biotyping was quite related to pathogenicity up to 99%. The biotype 1A strains were non-pathogenic, while all strains of biotype $1B{\sim}4$ showed pathogenicity with the exception of one strain belonged to type 1B. The esculin and salicin test results were completely close and correlated to pathogenicity up to 99%. The HEp-2 cell invasion and pyrazinamidase test were related to pathogenicity by 95%. Biochemical tests such as D-xylose fermentation, CRMOX agar test and autoagglutination in broth were effective as a support test. It is strongly recommended that sequencial esculin test and PCR test could be done to verify pathogenicity of Y. enterocolitica as the easiest and accurate procedure.

• Journal of Microbiology and Biotechnology
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• v.6 no.1
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• pp.7-11
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• 1996

MR-387Al (ARPA-Val-Pro) and A2 (AHPA-Val-Hyp) were prepared as aminopeptidase N inhibitors through the synthesis of peptide MR-387A and B analogues which contained 3-amino-2-hydroxy-4-phenyl butanoic acid (ARPA) as a zinc-chelating moiety. They are competitive inhibitors of aminopeptidase N with inhibition constants(Ki) of 4.1 $\times 10^{-7}\;and 1.1 \times 10^{-6}$ M, respectively. MR-387Al also strongly inhibited aminopeptidase B of human myelogenous leukemia K-562 cell with $IC_50$ of 0.35 $\mu$ M. Inhibitions of aminopeptidase N activity by ARPA-bearing inhibitors of various peptide chain lengths also have been studied. $IC_ 50$ values of AHPA-Val (bestatin), ARPA-Val-Pro (MR-387Al) and ARPA-Val-Pro-Leu (MR-387C) compared against porcine kidney aminopeptidase N were 20.1, 0.60 and 0.08 $\mu$ M, respectively. These results support that a multiple interaction between the $S_1\to S'_3$ sites of aminopeptidase N and the $P_1\to P'_3$ of the inhibitor plays a crucial role in stabilizing strongly the enzyme-inhibitor complex.

• Biomedical Science Letters
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• v.14 no.1
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• pp.13-18
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• 2008

Excessive alcohol consumption is associated with increased risks of many diseases including cancer. Individuals who regularly consume excessive quantities of alcohol have a greater risk of developing head and neck cancers such as esophageal, pharyngeal and oral cavity cancers if they are deficient in ALDH2 expression compared to normal populations. We evaluated lipid peroxidation in Aldh2 +/+ and Aldh2 -/- mice after they had been subjected to acute ethanol exposure. Malondialdehyde(MDA) level in liver tissue was evaluated as a biomarker of oxidative lipid peroxidation. Although the ethanol treatment did not increase the hepatic MDA level both in Aldh2 +/+ mice and in Aldh2 -/- mice, the MDA level was significant higher in the Aldh2 -/- mice than in the Aldh2 +/+ group. The MDA level was also significantly correlated with olive tail moment in blood and the level of 8-OHdG in liver tissue. This is a strong evidence to support our hypothesis that oxidative stress is more intense in Aldh2 -/- mice than in Aldh2 +/+ mice. Our results suggest that ALDH2-deficient individuals may be more susceptible than wild-type ALDH2 individuals to ethanol-mediated liver disease, including cancer.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.684-695
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• 2010

Geographic information system (GIS) is being increasingly used for decision making, planning and agricultural environment management because of its analytical capacity. GIS and remote sensing have been combined with environmental models for many agricultural applications on monitoring of soils, agricultural water quality, microbial activity, vegetation and aquatic insect distribution. This paper introduce principles, vegetation indices, spatial data structure, spatial analysis of GIS and remote sensing in agricultural applications including terrain analysis, soil erosion, and runoff potential. National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA) has a spatial database of agricultural soils, surface and underground water, weeds, aquatic insect, and climate data, and established a web-GIS system providing spatial and temporal variability of agricultural environment information since 2007. GIS-based interactive mapping system would encourage researchers and students to widely utilize spatial information on their studies with regard to agricultural and environmental problem solving combined with other national GIS database. GIS and remote sensing will play an important role to support and make decisions from a national level of conservation and protection to a farm level of management practice in the near future.

• The Korean Journal of Community Living Science
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• v.18 no.1
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• pp.3-20
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• 2007

The purpose of this study was diagnosed to grasp the needs for the elderly-elderly care activity in rural areas. The research was conducted in questions and case studies in 7 farming villages. The major findings of this study as follows: First, the senior citizens in rural areas were not in good health, and the illnesses were revealed to be arthritis, high blood pressure, and diabetes. At the occurrence of an illness, they complained about the discomfort in doing daily life routines and expected eir spouses or offsprings to te care of them. Elders in the rural community responded that they had difficulty and financial problems in family maintenance. Leisure activities included watching TV, playing card/board games, or spending time without any specific activities. Second, recipients of elderly-elderly care expected to receive services from a woman in her 50s $1{\sim}2$ hours, once to twice a week. Third, the service providers wanted to be elderly-elderly care takers, hoping to work once a week for $2{\sim}3$ hours. They wanted the education for the elderly-elderly care to be once a week for 3 hours, with understanding the elderly, consultation, preventing senile dementia, and health as the contents.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2001.01a
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• pp.89-96
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• 2001

Agent-mediated electronic markets have been a grow-ing area of agent research and developmen tin recent year. There exist a lot of e-commerce sites on the In-ternet(e.g. Priceline, com, Amazon, com etc). These e-commerce site have proposed new business models for effective and efficient commerce activity. Intelli-gent agents have been studied very widely in the field of artificial intelligence, For purpose of this paper, an agent can act autonomously and collaboratively in a network environment on behalf of its users. It is hard for people to effectively and efficiently monitor, buy, and sell at multiple e-commerce sites. If we intro-duce agent technologies into e-commerce systems, we can expect to further enhance the intelligence of their support. In this paper, we propose a new coopera-tion mechanism among seller agents based on exchang-ing their goods in our agent-mediated electronic market system. G-Commerce. On G-Commerce, seller agents and buyer agents negotiate with each other. In our model, seller agents cooperatively negotiate in order to effectively sell goods in stock. Buyer agents coopera-tively form coalitions in order to buy goods based an discount proices. Seller agent's negotiation goods. Our current experiments show that exchanging mechanism enables seller agents to effectively sell goods in stock. Also, we present the Pareto optimality of our exchang-ing mechanism.

• BMB Reports
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• v.39 no.3
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• pp.270-277
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• 2006

Helices 4 and 5 of the Bacillus thuringiensis Cry4Ba $\delta$-endotoxin have been shown to be important determinants for mosquito-larvicidal activity, likely being involved in membrane-pore formation. In this study, the Cry4Ba mutant protein containing an additional engineered tryptic cleavage site was used to produce the $\alpha4$-$\alpha5$ hairpin peptide by an efficient alternative strategy. Upon solubilization of toxin inclusions expressed in Escherichia coli and subsequent digestion with trypsin, the 130-kDa mutant protoxin was processed to protease-resistant fragments of ca. 47, 10 and 7 kDa. The 7-kDa fragment was identified as the $\alpha4$-loop-$\alpha5$ hairpin via N-terminal sequencing and mass spectrometry, and was successfully purified by size-exclusion FPLC and reversed-phase HPLC. Using circular dichroism spectroscopy, the 7-kDa peptide was found to exist predominantly as an $\alpha$-helical structure. Membrane perturbation studies by using fluorimetric calcein-release assays revealed that the 7-kDa helical hairpin is highly active against unilamellar liposomes compared with the 65-kDa activated full-length toxin. These results directly support the role of the $\alpha4$-loop-$\alpha5$ hairpin in membrane perturbation and pore formation of the full-length Cry4Ba toxin.

• Journal of Environmental Science International
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• v.22 no.1
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• pp.91-98
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• 2013

In order to study the seasonal patterns and possible origins of air concentrations of volatile organic compounds(VOC), measurements were taken with GC-MS at 3 sampling sites in Jinju for 12 months from Mar. 2010 to Feb. 2011. Atmospheric VOC are sampled on tubes containing solid adsorbents(Tenax TA) with a time resolution of 2hrs. Composition and concentration of VOC are analysed with a GC system equipped with thermal desorption apparatus(ATD). The most abundant compound appeared to be Toluene, Ethylbenzene and m,p-Xylene. The mean concentrations of Benzene were 0.20 ppb at GN site, 0.18 ppb at DA site, and 0.25 ppb at SP site, respectively. VOC concentration showed a strong seasonal variation, with higher concentrations during the spring and lower concentrations during the summer. The results showed that monthly fluctuations in measured VOC concentrations depended on variations in the strength of sources, as well as on photochemical activity and meteorological conditions. In Jinju, the total VOC emissions for 2009 were estimated to be 4,407 ton/year by Clean Air Policy Support System(CAPSS). It is shown that solvent use 57.5%(2,534 ton/yr), waste treatment and disposal 23.3%(1,025 ton/yr), and mobil source-road traffic 12.2%(537 ton/yr) are the most significant anthropogenic source.

• Industrial Engineering and Management Systems
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• v.7 no.2
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• pp.171-181
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• 2008

Home care, known also as domiciliary care, is part of the community care service that is a responsibility of the local government authorities in the UK as well as many other countries around the world. The aim is to provide the care and support needed to assist people, particularly older people, people with physical or learning disabilities and people who need assistance due to illness to live as independently as possible in their own homes. It is performed primarily by care workers visiting clients' homes where they provide help with daily activities. This paper is concerned with the dispatching of care workers to clients in an efficient manner. The optimized routine for each care worker determines a schedule to achieve the minimum total cost (in terms of distance traveled) without violating the capacity and time window constraints. A collaborative population-based meta-heuristic called Particle Swarm Optimization (PSO) is applied to solve the problem. A particle is defined as a multi-dimensional point in space which represents the corresponding schedule for care workers and their clients. Each dimension of a particle represents a care activity and the corresponding, allocated care worker. The continuous position value of each dimension determines the care worker to be assigned and also the assignment priority. A heuristic assignment scheme is specially designed to transform the continuous position value to the discrete job schedule. This job schedule represents the potential feasible solution to the problem. The Earliest Start Time Priority with Minimum Distance Assignment (ESTPMDA) technique is developed for generating an initial solution which guides the search direction of the particle. Local improvement procedures (LIP), insertion and swap, are embedded in the PSO algorithm in order to further improve the quality of the solution. The proposed methodology is implemented, tested, and compared with existing solutions for some 'real' problem instances.

• Journal of Advanced Navigation Technology
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• v.18 no.5
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• pp.409-414
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• 2014

Since the five-day workweek has implemented and the spare time increased, the tourist industry has been showing the growth with quality. The tourist industry takes center stage as the twenty-first century higher value-added business on the strength of electronic communication development. Especially as being surrounded by water on three sides and national income has incremented, people have the interest in marine leisure industry. The Ministry of Maritime Affairs and Fisheries carries out a plan to promote marine tourism promotion plan through the significant policy support. Also, they makes an effort to lure the tourist through blending cultures. Therefore, through the 360 degree camera, the activity that mobilizes the policy fund can be monitored rightly and the application strategy that is useful to promote a higher value-added tourist industry can be suggested.