• 한국응용과학기술학회지
• /
• 제37권1호
• /
• pp.56-65
• /
• 2020

본 연구는 ABTS radical 소거 활성을 통한 항산화 활성, RAW 264.7 대식 세포에서의 세포 독성, DCF-DA assay을 통한 세포 내 ROS 생성 억제 효과, 항균력을 측정하여 구아바 잎 추출물이 화장품 소재로써의 활용 가능성을 검토하고자 하였다. 실험 결과, 구아바 잎 추출물의 우수한 ABTS radical 소거능을 확인하였다. RAW 264.7 대식세포에서의 세포 독성은 나타나지 않았으며 세포 내에서 ROS 생성량은 농도 의존적으로 감소하는 억제효과를 확인하였다. 또한 구아바 잎 추출물의 항균력 분석에서는 피부상재균인 S. aureus, E. coli, C. albicans, P. acnes 균주들에서 항균 활성이 확인되었으며, 각 균주에 대한 최소 저해 농도(MIC)는 대체로 0.25 - 1 mg/mL의 수준으로서 P. acnes ≃ S. aureus < E. coli < C. albican 순의 낮은 농도로 측정되었다. 이와 같은 결과를 통해 구아바 잎 추출물의 항산화 활성과 세포 내 ROS 생성 억제 효과, 피부에 염증을 유발하는 피부상재균에 대한 항균력이 확인됨에 따라 독성이나 부작용이 없는 기능성 화장품 소재로써의 활용이 가능할 것으로 사료된다.

• Journal of Applied Biological Chemistry
• /
• 제44권2호
• /
• pp.77-83
• /
• 2001

For defining the possibilities of the commercial mass liquid culture of Cordyceps sinensis, the pharmacological activities of mycelia were analyzed. The mycelium of C. sinensis consists of carbohydrate (5.1%) and fat (1.3%), and contains a low content of protein (0.7%) and ash (0.5%), and 92.4% moisture. The molecular sugar ratio of carbohydrate was composed mainly of glucose, mannose (1.0 : 0.9), in addition a small amount of galactose and arabinose (0.2 : 0.1). The cellular materials of mycelia were fractionated into ethylacetate (EA), MeOH (M) and hot-water extract fraction (HW). HW fraction showed the most potent intestinal immune system modulating activity, anti-coagulant activity, and anti-complementary activity, and M fraction had the inhibition activity of radical generation as effective as genistine. These results reveal that the mycelium of liquid cultured C. sinensis showed pharmacological activities and could be used for commercial purpose.

• 국제학술발표논문집
• /
• The 1th International Conference on Construction Engineering and Project Management
• /
• pp.791-795
• /
• 2005

A simulation model is proposed to evaluate the effect of correlations between activity durations on the overall project duration. The proposed model incorporates NORTA, a recent developed statistical method, into the simulation process to allow arbitrarily specified marginal distributions for activity durations and any desired correlation structure. The generality is of practical value when systematic data is not available and planners have to rely on arbitrary experts' estimation, which may involve a mixed situation when some activity durations are continuously distributed whereas others are discrete outcomes. The proposed model is validated by showing that the correlation coefficients of the simulation results are close to the originally specified ones. The simulation results are compared to two conventional approaches: PERT and simulation without correlation. The comparisons illustrate that the proposed model can provide important management information, which would otherwise be distorted due to the neglect of the correlations between activity durations.

• 대한영양사협회학술지
• /
• 제12권2호
• /
• pp.127-145
• /
• 2006

The purpose of this study is to develop an educational program for Korean traditional food culture, which is consisted of diverse activity factors and educational contents of the elementary students, and to transfer Korean traditional food culture to the next generation effectively. We have developed the program on the basis of FIB(Fun Integrated Behavior) approach derived from the activity-based education theory. This program is consisted of 32 subjects related with Korean traditional food culture, which is planned to perform in special activity classes for 2 semesters. An instruction guidebook for teachers and a workbook for students have been also developed. The traditional food culture educational program which is developed in this study could be used as a practical educational tool in 'dietary life and culture class' or 'special activity class' which is recently being introduced as the nutrition education curriculum in the elementary school. Furthermore, we expect this program to be able to contribute to transferring Korean traditional food culture to the next generation and reinforcing the elementary schoolers' diet behavior through inspiring them with a realization of the superiority of Korean traditional food culture.

• 한국약용작물학회지
• /
• 제19권3호
• /
• pp.177-184
• /
• 2011

This study was performed to assess the antioxidant activities and whitening effects of Agrimonia pilosa Ledeb on melanin synthesis. The whitening effects of Agrimonia pilosa Ledeb water extracts were examined by in vitro mushroom tyrosinase assay and B16BL6 melanoma cells. We assessed inhibitory effect of Agrimonia pilosa Ledeb water extract on expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effect of Agrimonia pilosa Ledeb onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activitie. Our results indicated that Agrimonia pilosa Ledeb water extract effectively inhibited free radical generation. In DPPH and hydroxy radical scavenging activity, Agrimonia pilosa Ledeb water extract had a potent anti-oxidant activity in a dose-dependent manner. They significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. Also, Agrimonia pilosa Ledeb suppressed the expression of tyrosinase in B16BL6 melanoma cells. These results show that Agrimonia pilosa Ledeb inhibited melanin production on the melanogenesis. The underlying mechanism of Agrimonia pilosa Ledeb on whitening activity may be due to the inhibition of tyrosinase activity. We suggest that Agrimonia pilosa Ledeb may be useful as new natural active ingredients for antioxidant and whitening cosmetics.

• 인터넷정보학회논문지
• /
• 제14권2호
• /
• pp.25-34
• /
• 2013

본 논문에서는 워크플로우 기반 인적 자원의 소속성 분석을 위한 업무-수행자 이분 행렬 생성 알고리즘을 제안한다. 워크플로우 기반 인적 자원은 워크플로우 관리 시스템에 의해 관리되는 조직의 모든 수행자들을 말하며, 워크플로우 모델의 실행 과정에서 특정 업무 집합에 참여하게 된다. 이러한 워크플로우 모델에 정의된 수행자들과 업무들과의 소속성을 나타내는 소셜 네트워크를 업무-수행자 소속성 네트워크라 정의하였으며, 본 논문에서 제안하는 알고리즘은 워크플로우 모델로부터 발견된 업무-수행자 소속성 네트워크 모델(APANM)에 대한 이분 행렬을 생성하기 위한 알고리즘이다. 결론적으로, 알고리즘에 의해 생성된 업무-수행자 이분 행렬은 중심성(centrality), 밀집도(density), 상관 관계(correlation)와 같은 다양한 소셜 네트워크 관련 속성들을 분석하는데 적용될 수 있으며, 이를 통해 워크플로우 기반 인적 자원의 소속성에 대한 유용한 지식을 획득할 수 있다.

• 한국작물학회지
• /
• 제39권2호
• /
• pp.180-186
• /
• 1994

콩의 lipoxygenase 활성이 낮은 품종의 육성을 위한 유전육종학적 기초연구의 일환으로 서울대학교 농학과에서 유지해오고 있는 품종 및 계통과 국내 수집계통 등 507점을 공시재료로 하여 lipoxygenase 활성의 변이, 그리고 잡종초기세대를 통한 lipoxygenase 활성의 유전 및 선발효과를 조사한 결과는 다음과 같다. 공시품종 및 계통들의 lipoxygenase 활성은 50~670 unit (unit : $\Delta$0.001/min /mg, 234nm) 의 범위(507품종 및 계통)였으며 평균 350unit로 나타났다. 수집지역과 종실색 구분에 의한 각 그룹별 lipoxygenase 활성의 평균간 차이가 없었으며 성열이 이른 계통의 집단이 lipoxygenase 활성평균이 낮았다. Lipoxygenase 활성은 양적인 유전현상을 나타냈으며 활성을 높게하는 유전자의 상가적 효과가 우성효과보다 큰 것으로 나타났고 활성을 높게하는 유전자와 낮게하는 유전자의 분포비율은 비슷한 것으로 추정되었다. 추정된 협의의 유전력과 광의의 유전력은 0.78($H_n$), 0.86($H_b$)이었고, 선발효율은 고, 저 양 방향으로의 조기세대 선발에 의해 원집단에 비해 높은 방향의 선발에서 29.7~44.7%증가하였고 낮은 방향의 선발에서 21.8~27.3% 저하되었으며 모든 경우에 유의하게 선발효과가 있었다.

• 대한본초학회지
• /
• 제26권2호
• /
• pp.17-23
• /
• 2011

Objectives : This study aims at examining the effect of the fermentative extract of root of Sophorae Radix on the immuno-modulating activity. Methods : Cell viabilities were measured by MTT assay. Effect of SFS on nitric oxide(NO), hydrogen peroxide production from RAW 264.7 cells was accessed by Griess reagent assay. Effect of SFS on productions of inflammatory cytokines such as TNF-${\alpha}$, IL-6 in LPS-induced RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. Results : The results of the experiment are as follows. 1. As a result of carrying out MTT assay to check the cellular toxicity of the fermentative extract of Sophorae Radix. There was not any excessive toxicity to the macrophage when the fermentative extract of root of Sophorae Radix was treated in different concentrations. 2. The fermentative extract of Sophorae Radix increased the generation of hydrogen peroxide in the macrophage and significantly restored the suppression of the generation of the hydrogen peroxide in the macrophage induced by LPS. 3. The fermentative extract of Sophorae Radix reduced the generation of NO in the macrophage and significantly suppressed the increase of the generation of NO in the macrophage induced by LPS. 4. The fermentative extract of Sophorae Radix significantly decreased the amount of TNF-${\alpha}$ generated in the macrophage induced by LPS when it was $25{\mu}g/mL$ or higher. Conclusion : These results suggest that SFS has anti-inflammatory moiety related with its inhibition of NO, hydrogen peroxide, TNF-${\alpha}$, IL-6, in macrophage led by LPS.

• 한국농공학회논문집
• /
• 제55권5호
• /
• pp.25-35
• /
• 2013

In swine house, dust generation comes from various sources and is known to be harmful both for the animals and the farmers because the dust contains biological and gaseous matters. When farmers are constantly exposed to the dusts, they can suffer chronic or acute respiratory symptoms and have high probability of manifesting various diseases. To address this problem, understanding of the mechanism of dust generation is very important. In this paper, the dust concentration of inhalable, respirable, TSP and $PM_{10}$ were monitored and analyzed according to the pig-activity level, ventilation quantity and feeding method in fattening pig house. From the measured results, in case of the concentration of TSP, an inverse-linear relation with ventilation rate ($R^2=0.88$) and linear relation with the installation height of feed supply pipe ($R^2=0.73$) were determined. However in case of the concentration of $PM_{10}$, no particular relationship with the variables was observed. Using the concentration of inhalable and respirable dust based on the pig-activity level, multi-variate regression analysis was conducted and results have shown that the movement of pigs can contribute to the dust generation (p＜0.05, $R^2=0.71$, 0.61). The relationship determined between dust generation and environmental variables investigated in this study is very significant and useful in conducting dust-reduction researches.

• 약학회지
• /
• 제53권1호
• /
• pp.1-5
• /
• 2009

This study was performed to investigate the effects of acteoside and isoacteoside isolated from Clerodendron trichotomum Thunberg on melanin production in B16 melanoma cells. In DPPH radical scavenging activity, acteoside and isoacteoside had a potent anti-oxidant activity in a dose-dependent manner. Both acteoside and isoacteoside dose-dependently inhibited silica-induced ROS (reactive oxygen species) generation in B16 melanoma cells. They significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. The inhibitory effect of acteoside was more potent than that of isoacteosidee. In Western blot of tyrosinase, acteoside inhibited MSH-induced tyrosinase expression in B16 melanoma cells, which is related to the inhibitory action of acteoside on tyrosinase activity and melanin production. These results show that acteoside and isoacteoside from Clerodendron trichotomum Thunberg has a potent antioxidant activity and whitening activity. The underlying mechanism of acteoside on whitening activity may be due to the inhibition of tyrosinase activity and tyrosinase expression.