• Title/Summary/Keyword: Activity Generation

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31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

  • Kim, Hyun-Won
    • Bulletin of the Korean Chemical Society
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    • v.32 no.1
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    • pp.183-185
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    • 2011
  • Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

Biodiversity and Enzyme Activity of Marine Fungi with 28 New Records from the Tropical Coastal Ecosystems in Vietnam

  • Pham, Thu Thuy;Dinh, Khuong V.;Nguyen, Van Duy
    • Mycobiology
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    • v.49 no.6
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    • pp.559-581
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    • 2021
  • The coastal marine ecosystems of Vietnam are one of the global biodiversity hotspots, but the biodiversity of marine fungi is not well known. To fill this major gap of knowledge, we assessed the genetic diversity (ITS sequence) of 75 fungal strains isolated from 11 surface coastal marine and deeper waters in Nha Trang Bay and Van Phong Bay using a culture-dependent approach and 5 OTUs (Operational Taxonomic Units) of fungi in three representative sampling sites using next-generation sequencing. The results from both approaches shared similar fungal taxonomy to the most abundant phylum (Ascomycota), genera (Candida and Aspergillus) and species (Candida blankii) but were different at less common taxa. Culturable fungal strains in this study belong to 3 phyla, 5 subdivisions, 7 classes, 12 orders, 17 families, 22 genera and at least 40 species, of which 29 species have been identified and several species are likely novel. Among identified species, 12 and 28 are new records in global and Vietnamese marine areas, respectively. The analysis of enzyme activity and the checklist of trophic mode and guild assignment provided valuable additional biological information and suggested the ecological function of planktonic fungi in the marine food web. This is the largest dataset of marine fungal biodiversity on morphology, phylogeny and enzyme activity in the tropical coastal ecosystems of Vietnam and Southeast Asia. Biogeographic aspects, ecological factors and human impact may structure mycoplankton communities in such aquatic habitats.

Effects of Environmental Variables on Hydrogen Generation from Alkaline Solutions using used Aluminum Cans (알칼리 용액에서 알루미늄 재활용 캔을 이용한 수소생산에 미치는 환경 인자의 영향)

  • Yun, Kwi-Sub;Park, Chan-Jin
    • Transactions of the Korean hydrogen and new energy society
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    • v.22 no.1
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    • pp.29-34
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    • 2011
  • This study examined the effect of environmental variables, such as the NaOH concentration and solution temperature, on the rate of hydrogen generation from NaOH solutions through the corrosion of used aluminum cans as a potential candidate material for the safe and economic production of hydrogen. Corrosion of the used aluminum cans was promoted by increasing the NaOH concentration and solution temperature because of the loss of aluminum passivity. The measured rate of hydrogen generation from the NaOH solutions increased with increasing NaOH concentration due to the catalytic activity of NaOH in the hydrolysis process. However, at higher solution temperatures, the rate of hydrogen generation rate was less affected by the NaOH concentration than that at lower temperature.

The Leisure behaviors of single households in Korea : A generational analysis (1인 가구의 세대별 여가행태 분석)

  • Jeong, Yeongkeum;Yoon, Soyoung
    • Journal of Family Resource Management and Policy Review
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    • v.22 no.4
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    • pp.1-15
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    • 2018
  • The purposes of this study is to analyze the leisure behaviors of single households in Korea on the basis of generation, and identify leisure characteristics or factors that influence levels of happiness in these households. The sample comprised 1,144 single householders, among whom 74 belong to the teenage generation, 288 are of the eco generation, 154 belong to generation X, 104 are baby boomers, and 524 are of the older generation. Various leisure characteristics were compared across the generations, namely, conditions for leisure, degree of leisure participation, level of work-leisure balance, persistence of participation, and type of activity. Results showed differences in leisure characteristics and leisure factors that influence levels of happiness among the generations.

Development of a Digital Storytelling Activity Model for Digital Generation (디지털 세대를 위한 디지털 스토리텔링 활동 모형 개발)

  • Jo, Miheon
    • Journal of The Korean Association of Information Education
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    • v.21 no.6
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    • pp.647-656
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    • 2017
  • Digital storytelling provides students with opportunities to plan and compose a story, and create a storytelling material using various digital technologies. Through digital storytelling activities, students can enhance competencies such as creativity, communication, critical thinking and collaboration, which are required for the digital generation in the future. The purpose of this research was to develop a digital storytelling model that guides students' activities related to the planning of a story and the creation of a digital material. The model classified 4 stages of activities(i.e. 'Preparing Activity', 'Planning and Composing a Story', 'Producing Storytelling Material' and 'Making a Presentation and Evaluating Storytelling Material'), and provided instructional guidances for each stage. Also various forms were presented for story planning, story table, evaluation among students and individual reflection journal.

Convergence research on the possibility of development of oral care products using the anti-plaque activity of natural essential oils against Streptococcus mutans (천연에센셜오일의 Streptococcus mutans에 의한 치석형성 억제 활성을 이용한 구강관리제품 개발 가능성에 대한 융합연구)

  • Kim, Minhyung;Lee, So-Young;Min, Hee-Hong
    • Journal of the Korea Convergence Society
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    • v.9 no.7
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    • pp.149-154
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    • 2018
  • This article intended to examine the anti-plaque activity of 4 essential oils of Lavender, Tea tree, Eucalyptus, Lemongrass against Streptococcus mutans. In the results of measuring the anti-plaque effect against Streptococcus mutans, Minimum anti-adhesive concentration of Lavender oil was 1.0% and that of Tea tree, Eucalyptus, lemongrass essential oils was 0.5 %. Also, it was confirmed that the essential oils have the effect of inhibiting acid generation by Streptococcus mutans. It was confirmed that pH of the concentration was lowered by the acid generation under the MAC by measuring pH of the solution after incubating Streptococcus mutans and the essential oils in the thermostatic bath varying their concentration. From these results, the essential oils, particularly, Tea tree, Eucalyptus, Lemongrass essential oils are the natural material inhibiting the plaque generation and the potential that they can be used to develop the oral care products was confirmed.

Anti-Inflammatory Activity of Compounds from the Whole Plant of Patrinia saniculaefolia

  • An, Ren-Bo;Na, Min-Kyun;Min, Byung-Sun;Chang, Hyeun-Wook;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.17 no.2
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    • pp.90-94
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    • 2011
  • An in vitro bioassay-guide revealed that the methanol (MeOH) extract of the whole plant of Patrinia saniculaefolia (Valerianaceae) showed cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) dual inhibitory activity by assessing their effects on the production of prostaglandin $D_2$ ($PGD_2$) and leukotriene $C_4$ ($LTC_4$) in mouse bone marrow-derived mast cells (BMMCs). Phytochemical study of the MeOH extract of this plant led to the isolation of twelve compounds; ${\beta}$-farnesene (1), squalene (2), nardostachin (3), patridoid I (4), patridoid II (5), patridoid II-A (6), oleanolic acid (7), oleanonic acid (8), 23-hydroxyursolic acid (9), oleanolic acid 3-O-${\alpha}$-L-arabinopyranoside (10), oleanolic acid 3-O-${\beta}$-D-glucopyranoside (11), oleanolic acid 3-O-[${\beta}$-D-xylopyranosyl-(1${\rightarrow}$3)-${\beta}$-D-(6-O-butyl)glucuronopyranoside] (12). Among the compounds, 4 and 5 strongly inhibited both the COX-2-dependent $PGD_2$ generation with $IC_{50}$ values of 8.7 and 13.6 ${\mu}M$, respectively, and the generation of $LTC_4$ in the 5-LOX dependent phase with $IC_{50}$ values of 41.7 and 46.9 ${\mu}M$, respectively, which suggest that the anti-inflammatory activity of P. saniculaefolia might occur in part via the inhibition of both $PGD_2$ and $LTC_4$ generation by 4 and 5.

Antiplatelet Activity of NQ12 May Be Mediated by Inhibition of Cyclooxygenase and Generation of 12-HETE

  • Jin, Yong-Ri;Shin, Hye-Jun;Cho, Mi-Ra;Ryu, Chung-Kyu;Yun, Yeo-Pyo
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.278.1-278.1
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    • 2002
  • In the previous study, we reported that NQ12, a vitamin K antagonist. showed a potent antithrombotic and antiplatelet activities. In order to elucidate the antiplatelet activity of NQ12. we investigated the effect of NQ12 on arachidonic acid cascade parameters such as cPLA2. cyclooxygenase (COX), and the downstream production such as TxA2, PGD2 and 12-HETE. N012 inhibited COX activity in a concentration-dependent manner in U937 cells. (omitted)

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Designs for Development of Bra Tops for the New Senior Generation

  • Lim, Hosun;Cho, Hakyung
    • Fashion & Textile Research Journal
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    • v.15 no.3
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    • pp.358-363
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    • 2013
  • Due to recent changes in increased life expectancy, economic power and social development, the new senior generation (focusing on people in their fifties) represents a new market power with strong competitiveness. The new senior generation is the generation after the baby boomers, with characteristics that include a high-level of education, increased income, a high-level of consumption, increased assets, self-fulfillment desires and individuality. Demands in the senior fashion industry are expected to gradually increase and the underwear industry that targets the new senior generation is expected to grow rapidly. These demands will require product development for the new senior generation based on emotional and socio-cultural characteristics. The current new senior underwear market is under the process of R&D with a focus on body shape changes; however, there are limited studies on emotional and socio-cultural approaches. This study formulates a basic design plan for different age groups based on an analysis of the situation of bra wear (current inconveniences, demand and needs) by women in their fifties and over. Subsequently, a functional active bra top that considers activity and comfort was proposed for women in their fifties, an all-in-one foundation bra top that shapes the body was proposed for women in their sixties, and comfort bra tops that are comfortable and easy to put on and take off were proposed for women in their seventies and over.