• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.159-165
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• 1997

We collected 3,237 plant samples, mainly leaves of various trees, from many provinces in Korea and a total of 1,925 Bacillus thuringiensis isolates were obtained and characterized. The isolates were characterized in terms of crystal morphology, PAGE pattern of the toxin proteins, plasmids pattern, biochemical characteristics, and bioassay. The microscopic observation showed that 49.1% of the isolates have bipyramidal shape crystals, 7.1% of spherical shape crystals, 1.4% of rhomboidal shape crystals, and others have small or amorphous inclusions. The insecticidal activities of the spore-crystal mixtures of isolates were tested against Plutella xylostella, Bombyx mori, Culex pipiens, and Agelastica coerulea. Bioassay showed that 51.3% of the isolates were shown to be active; lepidopteran-specific (44.8%), dipteran-specific(4.9%) and coleopteran-specific (1.6%). The remainder(48.8%) did not show any activity against the insects we tested. Interestingly though, some of these non-active isolates were shown to have bipyramidal crystals. By serotyping 22 isolates of our collection, we found that there are various kind of subspecies such as aizawai, amagiens, canadensis, darmstadiensis, galleriae, finitimus, kurstaki, morrisoni and neoleonensis, and three isolates have been classified into a new serotype, H49, and one of them, the type strain, named subsp. muju. From this study it was found that phylloplane is a good source for the isolation of Bacillius thuringiensis, and Bacillus thuringiensis is distributed widely in Korea.

• Journal of Internet Computing and Services
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• v.18 no.1
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• pp.37-41
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• 2017

In this paper, a platform was proposed that can service the streaming of location based real-time video-clip for smart-travel-services to work with PPL(Product Placement). The location information of drama like as LTE GPS and Beacon were used to provide the drama video with the PUSH service. And PPL DB with the attributes like as picture, video, music and URI(Uniform resource Identifier) was designed. As a result, this platform could provide the location based video streaming service that could replace the original PPL properties, color or shape, with the other properties by the interactive tagging the PPL DB in drama scenes. The proposed platform could provide the location based real-time video streaming service that can alter the PPL properties with the request of service provider.

• IE interfaces
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• v.21 no.2
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• pp.229-236
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• 2008

Recently, ubiquitous computing paradigm considers as a tool for making innovation and competitive strength in manufacturing industry like other industries. Particularly, the location-based service that enables us to trace real-time logistics make effective management of schedules for inventory control, facilities and equipments, jobs planning, and facilitate the processes of information management and intelligence, which relate with ERP and SCM in organizations. Our study tries to build the location-based system for products of semiconductors in manufacturing place and suggests the good conditions and effective tracking procedures for positions of products. Our study show that the system is good for the saving of time in tracking products, however, it has to be improved in terms of accuracy. The study verifies the application of RFID technology in manufacturing industry and suggests the improvement of photograph process through RFID. In addition, our research introduces the future operation of FAB in semiconductors' processes that relate with real-time automation and RFID in manufacturing company.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11a
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• pp.376-378
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• 2005

유비쿼터스 컴퓨팅을 구현하려면 위치인식 기술과 이를 기반으로 하는 다양한 위치 인식시스템이 필수적이다. 그러나 복잡한 실내 환경에서는 Global Positioning System (GPS)의 이용이 불가능하며 실내 위치 측정을 위한 전문적인 시스템이 필요하다. RFID 기술은 적은 시설투자와 낮은 비용으로 위치 측정 시스템을 구축할 수 있는 가능성을 제공한다. RFID기술을 이용한 실내 위치 측정 시스템 LANDMARC는 기타 시스템과 달리 참고태그라는 개념을 도입하여 RFID리더기의 배치 수량을 줄이는 동시에 위치 측정의 정확성을 보장함으로써 시스템을 도입하는데 소요되는 비용을 절감하였으며 현실에서의 높은 사용 가능성을 제공한다. 본 논문에서는 기존의 LANDMARC시스템을 분석하여 문제점들을 해결하고 실내에서의 위치측정의 정확도를 진일보 향상시킬 수 있는 보다 효율적인 방안을 제안했다. 실험 분석 결과 동일한 실내 환경하에서 본 논문에서 제안하는 방법이 기존의 LANDMARC 시스템에 비하여 더 높은 정확성을 보장함을 입증하였다.

• Proceedings of the Korea Contents Association Conference
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• 2006.11a
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• pp.560-564
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• 2006

Managing articles on exhibition in the museum these days are generally conducted in manual. With the reason, profound time as well as money is spent without generating proper results and this matter is regarded as a serous problem to be solved. Therefore, in this thesis, a suggestion to solve the consequently difficulty will be provided. In the suggested solution, both portable and fixed RFID readers and an active tag using 900Mhz frequency range are used. In constant, the methods of understanding realtime management of exhibited articles and integrated managing for museum would be developed effectively.

• BMB Reports
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• v.29 no.3
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• pp.183-191
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• 1996

DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.

• Biotechnology and Bioprocess Engineering:BBE
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• v.2 no.2
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• pp.86-89
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• 1997

Epidermal growth factor(EGF) known as a urgastrone is a powerful mitogen with a wide variety of possibilities for medical usages. A mature EGF coding region was isolated from human prepro-EGF sequence by a conventional PCR and cloned into pQE vector in which the gene product was supposed to be expressed with 6$\times$His tag for the subsequent purification. The recombinant mature EGF was expressed in M15[Rep4], an Escherichia coli host strain, in amount of 30-40% of total proteins pressent in E. coli extract by the addition of isopropylthio-$\beta$-galactopyranoside (IPTG). The recombinant EGF purified using a Ni2+-NTA affinity colume chromatography was active in its ability to induce phosphorylation on tyrosine residues of several substrate proteins when murine NH3T3 and human MRC-5 fibroblast cells were stimulated with it. This work may provide the basic technology and information for the production of recombinant EGF.

• 한국정보컨버전스학회:학술대회논문집
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• 2008.06a
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• pp.75-86
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• 2008

This paper presents the design, implementation, and evaluation of the RFID Guardian, the first-ever unified platform for RFID security and privacy administration. The RFID Guardian resembles an "RFID firewall", enabling individuals to monitor and control access to their RFID tags by combining a standard-issue RFID reader with unique RFID tag emulation capabilities. Our system provides a platform for coordinated usage of RFID security mechanisms, offering fine-grained control over RFID-based auditing, key management, access control, and authentication capabilities. We have prototyped the RFID Guardian using off-the-shelf components, and our experience has shown that active mobile devices are a valuable tool for managing the security of RFID tags in a variety of applications, including protecting low-cost tags that are unable to regulate their own usage.

• Journal of the Society of Naval Architects of Korea
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• v.47 no.6
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• pp.836-842
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• 2010

Safety system including location monitoring system for blasting workers was studied. Positioning performance of the location monitoring system was highly dependent on communication protocol and the number of access points in the blasting cell. RTLS(Real Time Location System) is an important technology to develop the location information of workers and variously used to enhance workers safety. Location monitoring system with Cell-ID and RSSI wireless communication technology was verified to have a proper positioning performance for the steel block application.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.970-972
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• 2010

ERP is an integrated information system to manage all human and material resources in offices. Therefore, the business competitiveness is reinforced. The best requirement of ERP is the real time data collection and analysis in ubiquitous environment. Passive information collection is a problem in existing ERP system. To solve that problem, active system is proposed using RFID Tag and Reader. From the simulation results, we confirm that resource information is acquired and event message is occurred after fixed time. In this paper, the real time management system of industry resource with RFID collects resource information actively instead of passive information collection and supports the event according to the schedule.