• The Plant Pathology Journal
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• 제16권4호
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• pp.189-199
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• 2000

To search for the antifungal substances, various actino-mycete isolates were obtained from various soils of Korea using plate dilution method on the humic acid vitamin agar plates. In the screening procedures using a dual culture method, 32 actionomycete isolates were selected, which showed the inhibitory activity against mycelial growth of plant pathogenic fungi Altirnaria mali, Colletotrichum gloeosporides, Fusarium oxysporum f.sp. cucumerinum, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. Bioassay of the crude extracts from culture filtrates and mycelial mets revealed that 12 antagonistic actionomycetes produced highly active antifungal substances. Actinomycete strain S5-55 which showed the substantial antifungal activity against the tested fungi was selected for production of the antifungal substances. Based on the cytochemical and morphological characteristics, strain S5-55 was identified as a Streptomyces species. The results of the numerical identification using the TAXON program confirmed that Streptomyces strain S5-55 was identical with Streptomyces humidus including in TAXON major cluster 19. The production of antifungal substance was most favorable when S. humidus strain S5-55 was cultivated for 10 dats on soluble starch broth supplemented with $K_2$HPO$_4$. The antifungal substances active against the plant pathogenic fungi P. capsici and M. grisea were partially purified using $\textrm{C}_{18}$ reversed-phase column chromatography.

• 한국토양비료학회지
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• 제25권4호
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• pp.401-406
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• 1992

우리나라 28개 지역(地域) 논밭 토양에서 수집한 시료에서 방선균(放線菌) 108주를 분리하고, 고추역병균, 참깨역병균 및 채소입고병균에 종합적으로 항균력을 갖는 Streptomyces sp. A-2를 선발하여 그 활성물질(活性物質)의 특성을 조사한 결과는 다음과 같다. 1. G.Y.B., $M\ddot{u}eller$, B.H.I., Czapek 등의 배지중에서 길항성(拮抗性)의 효과가 가장 잘 이전되는 배지는 G.Y.B. 및 B.H.I. 배지이었고, 주(主) 활성물질(活性物質)은 에칠아세테이트로 이전(移轉)되는 군과 수층(水層)에 잔존하는 군으로 구분되었으며 이들 물질은 열(熱)과 압력(壓力)에 매우 강한 결태형태(結台形態)를 보였다. 2. 길항성(拮抗性) Streptomyces sp. A-2에 의해 배양된 여액의 pH를 3이나 12로 조정하여 길항물질을 추출하여 3개 식물병원균에 대한 길항력 검정을 실시한 결과 pH를 달리한 추출법에서 별다른 차이점이 없었다. 이들 분획의 UV 스펙트럼은 폴리엔 길항활성의 마크로라이드의 그것과 유사하였으므로 폴리엔계 항생제가 이 균주의 길항성에 관련하는 주(主) 물질(物質)이라고 추정되었다.

• 한국정밀공학회지
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• 제1권2호
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• pp.24-32
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• 1984

The object of the study is to discuss the effect of magic ink as a surface active substance on the mechanism of chip formation in oblique cutting. The Rehbinder effect has been known as a phenomenon that the mechanical strength reduces when the metal is coated with some surface active substances. In order to interpret these surface effects defined by Rehbinder, the influence on the shear strength of shear plane by coating surface active substances, cutting force by the depth of cut, surface roughness and hardness ratio were observed. The results are as follows: 1. By coating the magic ink on free surface of the forming chip, the effective shear angle increases, and the cuttinbg force and the deformed chip thickness decreases. 2. With the large inclination angle the effective shear angle increases, and the specific cutting force and the friction angle decrease. 3. Cutting of the coated surface improves the surface roughness and the hardness ratio drops, which means another Rehbinder effect.

• Journal of Dairy Science and Biotechnology
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• 제31권2호
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• pp.195-200
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• 2013

The ripening of cheese allows for the development of characteristic taste and flavour, nutritional substances, bio-active components and texture, helping to improve quality. Many different microbiological, biochemical and nutritional changes occur during the process depending on the quality of raw milk, added cultures and enzymes, as well as specific processing and ripening conditions. During the ripening lactose is hydrolyzed to lactic, propionic and acetic acid, helping to reduce potential effects of the problem of lactose intolerance. Fat is hydrolyzed to butyric, propionic and conjugated linoleic acid, which function as bio-active substances. Protein is hydrolyzed to different peptides and amino acids which all show various bio-activities. However, errors of cheese ripening can happen and affect the quality of the product. To guarantee good quality cheese the process needs to be managed carefully with the right microbes used and ensuring cleanliness of processing facilities, staff, ventilation and hazard analysis and critical control points (HACCP). Research into and controlling of ripening technology is crucial for producing high quality cheeses.

• KSBB Journal
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• 제8권3호
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• pp.300-305
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• 1993

옥수수 종설에 존재하는 brassinosteroid 활생물질 을 탐색하기 위하여 MeOH로 추출하고, 이 추출물을 rice inclination test를 지표로 용매분획, silica g gel adsorption chromatography, Sephadex LH-20 c chromatography, charcoal adsorption chromato­g graphy, bondesil chromatography 등의 수법으로 b brassinosteroid 활생물질을 정제한 다음, silica gel 흡착 chromatography로 활생 성분을 분리하고 H HPLC에 의해 활성 본체를 구명한 결과, castas­t terone과 teasterone이 동정되었는데, 대부분 cas­t tasterone형태로 존재하였다. 옥수수 종실에 함유된 b brassinosteroid함량은 brassinolide로 환산하여 생 체중량 g당 3-8ng 수준이였다.

• 한국해양환경ㆍ에너지학회지
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• 제11권4호
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• pp.214-220
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• 2008

선박평형수 관리 협약에서 활성물질을 사용하는 선박평형수 처리장치는 IMO가 제정한 절차에 따라 IMO로부터 승인을 받아야하는 것으로 되어 있다. 협약에서 활성물질이란 유해 수중 생물과 병원균에 대하여 바이러스나 균류를 포함한 일반적인 또는 특정한 작용을 하는 물질 또는 생물을 말한다고 되어 있다. IMO 해양환경 보호위원회에서는 2008년 10월까지 13개의 선박평형수 처리장치에 기본승인을 부여하였고 4개의 처리장치에 최종승인을 부여하였다. 본 논문에서는 "활성물질을 사용하는 선박평형수 관리시스템의 승인을 위한 절차서(G9)"와 "GESAMP-BWWG의 정보 수집과 업무 수행을 위한 방법"에 근거하여 기본승인과 최종승인의 절차와 문서의 요소를 고찰하였고, IMO로부터 기본승인 또는 최종승인을 받은 처리장치의 승인 내용을 요약하였으며, 이로부터 몇 가지 문제점을 제기하였다.

• 한국식품저장유통학회지
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• 제10권4호
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• pp.574-583
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• 2003

식품의 저장성 및 안전성 향상을 목적으로 필름이나 용기형태의 항균 기능성 포장재를 개발하고자 하는 노력이 지속되고 있으며, 아울러 새로운 고분자 및 항균 소재 탐색에 대한 연구도 활발히 진행되고 있다. 이와 관련하여 기존의 합성 고분자뿐만 아니라 생고분자에 각종 항균제를 첨가 또는 혼입하여 제조한 식품 포장용 항균성 필름이 최근 들어 주목받고 있는데, 이러한 항균성 필름은 담체로 사용된 고분자는 물론 항균제의 종류, 이들의 상호작용에 따라 항균효과 및 지속기간, 필름의 물성 등이 현저하게 달라진다. 따라서 보다 효과적인 식품 포장용 항균 기능성 필름의 제조기술 개발을 위해 발표된 문헌 자료를 토대로 항균필름 제조에 사용된 고분자 소재와 항균제의 종류, 항균활성 평가방법, 제조방법에 따른 항균효과 및 필름의 물성 변화 등 항균필름의 종합적인 항균성 평가 결과를 중심으로 정리하였다.

• Journal of Ginseng Research
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• 제47권3호
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• pp.408-419
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• 2023

Background: Ginsenoside compound K (CK), the main active metabolite in Panax ginseng, has shown good safety and bioavailability in clinical trials and exerts neuroprotective effects in cerebral ischemic stroke. However, its potential role in the prevention of cerebral ischemia/reperfusion (I/R) injury remains unclear. Our study aimed to investigate the molecular mechanism of ginsenoside CK against cerebral I/R injury. Methods: We used a combination of in vitro and in vivo models, including oxygen and glucose deprivation/reperfusion induced PC12 cell model and middle cerebral artery occlusion/reperfusion induced rat model, to mimic I/R injury. Intracellular oxygen consumption and extracellular acidification rate were analyzed by Seahorse multifunctional energy metabolism system; ATP production was detected by luciferase method. The number and size of mitochondria were analyzed by transmission electron microscopy and MitoTracker probe combined with confocal laser microscopy. The potential mechanisms of ginsenoside CK on mitochondrial dynamics and bioenergy were evaluated by RNA interference, pharmacological antagonism combined with co-immunoprecipitation analysis and phenotypic analysis. Results: Ginsenoside CK pretreatment could attenuate mitochondrial translocation of DRP1, mitophagy, mitochondrial apoptosis, and neuronal bioenergy imbalance against cerebral I/R injury in both in vitro and in vivo models. Our data also confirmed that ginsenoside CK administration could reduce the binding affinity of Mul1 and Mfn2 to inhibit the ubiquitination and degradation of Mfn2, thereby elevating the protein level of Mfn2 in cerebral I/R injury. Conclusion: These data provide evidence that ginsenoside CK may be a promising therapeutic agent against cerebral I/R injury via Mul1/Mfn2 mediated mitochondrial dynamics and bioenergy.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2020년도 봄 학술논문 발표대회
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• pp.189-190
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• 2020

The importance of indoor air quality management has recently been highlighted due to environmental problems such as indoor air pollution. Among indoor air pollutants, carbon dioxide occurs in cooking, heating, burning, and causes forgetfulness, dementia and amnesia. Radon, which occurs in building materials, soil and ground, is a type 1 carcinogen that causes lung cancer in the body through breathing. These substances can be released from the room through ventilation, but there is a limit to reducing the amount of indoor activity due to reduced ventilation conditions due to increased indoor activity time. However, these substances can be removed from the gas by adsorption. The purpose of this study was to identify the properties of granular active and powdered active white soil and mix them to make cement-based active white soil adsorbent matrix for carbon dioxide, fine dust and radon gas adsorption, and to evaluate indoor air improvements according to the mixing scale. The results of the experiment showed that active carbon dioxide adsorption performance increased for carbon dioxide and radon as the exchange rate increased through physical adsorption. In particular, the higher the replacement rate of the granular active bag, the better adsorption performance was shown.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.296-304
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• 1994

Antibiotic substances were produced by Bacillus subtilis YB-70, a potential biocontrol agent found to suppress root-rot of eggplant (Solanum melonggena L) caused by Fusarium solani, in a dextrose glutamate medium and isolated by isoelectric precipitation. Partial purification was performed by column chromatography on silica gel with two solvent systems: chloroform-methanol and methanol-chloroform-water as eluting solvents, This active fraction YBS-1 s contained antifungal activity were soluble in ethanol, methanol, and water, but were not soluble in other solvents including acetone, butanol, ethyl ether, dimethylformamide, propanol, and etc. High performance liquid chromatography and thin layer chromatographic separation of YBS-1s showed that they have been composed of three biological active bands that were named YBS-1A, -1B, and -1C. The substances were stable to heat and resistant to protease. YBS-1s were active against a wide range of plant pathogenic fungi but did not inhibit the growth of bacteria and yeasts. They were not only fungicidal but also fungistatic against chlamydospores of F. solani. The $ED_{50}$ values for the chlamydospore germination and the germ-tube growth of F. solani were $O.725\mu\textrm{m}/ml\;and\;O.562\mu\textrm{m}/ml$, respectively. Microscopic observations proved the substances restricted the growth of phytopathogenic fungus F. solani by spore burst followed by dissolving of its germ-tube, and caused abnormal hyphal swelling after application to chlamydospores or growing hyphae. Cultural filtrate of B; subtilis YB-70 also suppressed the development of root-rot of eggplant in pot tests.