• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.65-71
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• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1185-1198
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• 2018

The nucleosomal organization of chromatin using histone proteins is a fundamental and ubiquitous feature of eukaryotic nuclei, with the major exception of dinoflagellates. Although a number of recent genomic and transcriptomic analyses have detected numerous histone genes in dinoflagellates, little is known about their expression. Here in, we aimed to investigate the expression pattern of histone genes under nutritional stress, and an attempt was made to detect histone expression at the protein level in Alexandrium pacificum. The presence of histones at the mRNA level was confirmed in this study by the amplification, cloning, and sequencing of 10 different genes. Relative expression profiling of these genes under different growth conditions was determined with real-time PCR and revealed considerable levels of histone transcription in nutritionally stressed cells. We were unable to detect the expression of histones at the protein level even after immunodetection and analysis using mass spectrometry, although a histone-like protein was detected as a major nuclear component. A. pacificum expresses multiple variants of histone, and protein sequences revealed both conservation and divergence with respect to other eukaryotes. We concluded that A. pacificum maintained an active transcription of histone genes within the cell, and enhanced expression of histone genes in nutritional stress strongly suggest that histones have functional significance in dinoflagellates, although expression at the protein level was below our current detection limits, which suggests a limited role of histones in DNA packaging. Finally, the plausible regulation of histone expression at the gene and protein levels in A. pacificum is discussed.

• International Journal of Aeronautical and Space Sciences
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• 제16권3호
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• pp.475-483
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• 2015

We propose a numerical scheme to simulate the time-domain echo signals at tracking radar for a realistic scenario where an EAD (expendable active decoy) and an airborne target are both in dynamic states. On various scenarios where the target takes different maneuvers, the trajectories of the EAD ejected from the target are accurately calculated by solving 6-DOF (Degree-of-Freedom) equations of the motion for the EAD. At each sampling time of the echo signal, the locations of the EAD and the target are assumed to be fixed. Thus, the echo power from the EAD can be simply calculated by using the Friis transmission formula. The returned power from the target can be computed based on the pre-calculated scattering matrix of the target. In this paper, an IPO (iterative physical optics) method is used to construct the scattering matrix database of the target. The sinc function-interpolation formulation (sampling theorem) is applied to compute the scattering at any incidence angle from the database. A simulator is developed based on the proposed scheme to estimate the echo signals, which can consider the movement of the airborne target and EAD, also the scattering of the target and the RF specifications of the EAD. For applications, we consider the detection probability of the target in the presence of the EAD based on Monte Carlo simulation.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.219-225
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• 2006

The rabbit pyrogen test and Limulus amoebocyte lysate (LAL) assay have been used to detect endotoxins present in vaccines. Currently, the rabbit pyrogen test is used to detect endotoxins in hepatitis B (HB) vaccines, even though the HB surface protein, which is the active ingredient, is overexpressed in and purified from eukaryotic cells that lack these endotoxins. Although the LAL clot assay is sensitive and reliable and can be used to replace the rabbit pyrogen test, its reaction is limited by the lack of responsiveness to the Gram-positive bacterial components. Furthermore, aluminum hydroxide in the HB vaccine can interfere with the LAL assay. In contrast, macrophages can detect the endotoxin as well as other pyrogens, and secrete $TNF-{\alpha}$. Therefore, this study was undertaken to examine the possibility of replacing the animal tests with a more efficient $TNF-{\alpha}$ secretion assay. With this in mind, we determined if aluminum hydroxide in the HB vaccines affects the $TNF-{\alpha}$ secretion assay. HB vaccines and the HB protein solutions spiked with lipopolysaccharide (LPS) produced the same level of dose-dependent $TNF{\alpha}$ secretion and temperature increase in rabbits, indicating that aluminum hydroxide in the HB vaccine does not interfere with the pyrogenic response in rabbits, nor does it interfere with $TNF-{\alpha}$ secretion. In addition, the $TNF-{\alpha}$ assay was found to be more sensitive than the LAL assay, and correlated well with the pyrogen test and the LAL assay. These results suggest that the $TNF-{\alpha}$ assay in RAW264.7 cells is a good substitute for the current pyrogen assays that are used for detecting LPS in HB vaccines as well as in other vaccines containing aluminum.

• 한국환경과학회지
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• 제29권9호
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• pp.885-905
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• 2020

This study described the characteristics of the upstream migration of salmon (Chum salmon, Oncorhynchus keta) along Yeon-gok Stream in the eastern coastal region of Korea from October 24 to November 9, 2018 using radio tag and data storage tag loggers for the detection of the locations of tagged salmon and measurement of water temperature. Tracking experiments were conducted and classified into four types (case 1 to case 4) depending on the release time and the number of salmon tracked. Experiments from case 1 to case 3 were classified depending on the number of salmon tracked into cases in which a single tagged salmon was tracked (case 1), a pair of tagged salmon was tracked (case 2), and salmon were tracked by different sex ratios (case 3). Experiments from cases 1 to 3 were conducted between 10 AM and 1 PM, and case 4 was conducted after 3:30 PM. Salmon moved and spawned in the downstream region of the Yeon-gok, where water temperature is higher than in other rivers and salmon return in Canada, Russia, Japan, and the U.S.A. Most of the radio-tagged salmon swam in deep and shaded areas during the day but actively moved upstream close to sunset, regardless of the release time. Females showed relatively more active movements than males during upstream migration.

• 한국동물위생학회지
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• 제37권3호
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• pp.151-156
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• 2014

Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared using sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms ($R^2$=0.6375) and stabilized farms ($R^2$=0.8928)] in sample-to-positive (S/P) ratio va lues. Among the 140 sera from negative farms, one sample was falsely positive by either of the ELISA kits. In conclusion, both of the ELISA kits showed a good correlation when applied on field samples collected from farms at various stages of PRRSV infection. Bionote ELISA or IDEXX ELISA gave a false positive result on 1 out of 140 negative samples so their specificity was calculated as 99.3%. Therefore, Bionote ELISA would be a good complementary and alternative method for IDEXX ELISA kit, and vice versa.

• 한국재료학회지
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• 제22권5호
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• pp.237-242
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• 2012

We report on the NO gas sensing properties of Al-doped zinc oxide-carbon nanotube (ZnO-CNT) wire-like layered composites fabricated by coaxially coating Al-doped ZnO thin films on randomly oriented single-walled carbon nanotubes. We were able to wrap thin ZnO layers around the CNTs using the pulsed laser deposition method, forming wire-like nanostructures of ZnO-CNT. Microstructural observations revealed an ultrathin wire-like structure with a diameter of several tens of nm. Gas sensors based on ZnO-CNT wire-like layered composites were found to exhibit a novel sensing capability that originated from the genuine characteristics of the composites. Specifically, it was observed by measured gas sensing characteristics that the gas sensors based on ZnO-CNT layered composites showed a very high sensitivity of above 1,500% for NO gas in dry air at an optimal operating temperature of $200^{\circ}C$; the sensors also showed a low NO gas detection limit at a sub-ppm level in dry air. The enhanced gas sensing properties of the ZnO-CNT wire-like layered composites are ascribed to a catalytic effect of Al elements on the surface reaction and an increase in the effective surface reaction area of the active ZnO layer due to the coating of CNT templates with a higher surface-to-volume ratio structure. These results suggest that ZnO-CNT composites made of ultrathin Al-doped ZnO layers uniformly coated around carbon nanotubes can be promising materials for use in practical high-performance NO gas sensors.

• 융합신호처리학회논문지
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• 제1권1호
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• pp.23-31
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• 2000

본 논문에서는 물체의 경계나 형태 추출을 위하여 레벨 세트 이론을 바탕으로 한 새로운 곡선 전개방법을 제안한다. 특히 전처리 과정에서 잡음의 효과적 처리를 위하여 기존의 필터 방식들이 가지는 단점인 경계 부분의 bluning 현상을 줄이고 정확한 에지 위치를 보존할 수 있는 비등방성 확산 필터(anisotropic diffusion filter)를 사용한다. 기존의 레벨 세트 방식이 수축이나 팽창 중 단지 한가지의 방식만 적용되어지는 반면, 제안한 방법은 물체의 경계 추출시 팽창과 수축이 통시에 가능하므로 특히 초기 곡선이 여러 물체에 걸쳐져 있는 경우에도 정확한 형태 추출이 가능하였다. 아울러 초기 곡선의 설정이 위치나 형태에 거의 제한을 받지 않기 때문에 추출을 원하는 영역이 아주 조금만 포함되어 있어도 정화한 형태 추출이 가능하였다.

• Journal of Periodontal and Implant Science
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• 제41권4호
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• pp.167-175
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• 2011

Purpose: Periodontal ligament (PDL) cell differentiation into osteoblasts is important in bone formation. Bone formation is a complex biological process and involves several tightly regulated gene expression patterns of bone-related proteins. The expression patterns of bone related proteins are regulated in a temporal manner both in vivo and in vitro. The aim of this study was to observe the gene expression profile in PDL cell proliferation, differentiation, and mineralization in vitro. Methods: PDL cells were grown until confluence, which were then designated as day 0, and nodule formation was induced by the addition of 50 ${\mu}g$/mL ascorbic acid, 10 mM ${\beta}$-glycerophosphate, and 100 nM dexamethasone to the medium. The dishes were stained with Alizarin Red S on days 1, 7, 14, and 21. Real-time polymerase chain reaction was performed for the detection of various genes on days 0, 1, 7, 14, and 21. Results: On day 0 with a confluent monolayer, in the active proliferative stage, c-myc gene expression was observed at its maximal level. On day 7 with a multilayer, alkaline phosphatase, bone morphogenetic protein (BMP)-2, and BMP-4 gene expression had increased and this was followed by maximal expression of osteocalcin on day 14 with the initiation of nodule mineralization. In relationship to apoptosis, c-fos gene expression peaked on day 21 and was characterized by the post-mineralization stage. Here, various genes were regulated in a temporal manner during PDL fibroblast proliferation, extracellular matrix maturation, and mineralization. The gene expression pattern was similar. Conclusions: We can speculate that the gene expression pattern occurs during PDL cell proliferation, differentiation, and mineralization. On the basis of these results, it might be possible to understand the various factors that influence PDL cell proliferation, extracellular matrix maturation, and mineralization with regard to gene expression patterns.

• 천문학회보
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• 제36권2호
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• pp.61.2-61.2
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• 2011

Low-ionization nuclear emission-line regions (LINERs) have been generally regarded to be powered by active galactic nuclei (AGNs), yet still a number of alternative explanations on the origin of LINER emission are suggested; for example, planetary nebulae nuclei of massive stars, supernovae shocks from death of massive stars, and old stellar populations. Interestingly, a majority of recent star formation early-type galaxies (ETGs) in local universe presents such LINER emission lines. Given that situation, revealing the true nature of LINERs is a crucial step to constrain the evolution path to quiescent ETGs. To resolve the issue, we use Keck/LRIS to obtain spatially resolved spectra on a carefully selected ETG. The ETG SDSS J091628.05+420818.7 at redshift z ~ 0.024 shows modest LINER emission line features without any detection of 21 cm radio continuum nor X-ray emission. We perform a stellar continuum subtraction and measure emission line strengths and their uncertainties for each spectrum from five apertures along the slit with size of 1 arcsecond (~0.5 kpc). We find that extended spatial distributions of four emission lines $H{\alpha}$, $H{\beta}$, [OIII]${\lambda}5007$, and [NII]${\lambda}6583$, and they can be explained by central emission blurring effect. We conclude that the emissions seem to be centrally concentrated, indicating the AGN-nature of LINERs.