• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.175-182
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• 2002

Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor $\alpha$ or $\beta$, and Gal4p transactivation domain (TAD)-co-activator AIB-1, SRC-1 or TIF-2 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and Gal4p binding site (GAL4 upstream activating sequence, GAL4$_{UAS}$). Expression of this reporter gene was dependent on the presence of estrogen or EDs in the culture medium. We found that the two-hybrid system with combination of the hER$\beta$ LBD and co-activator SRC-1 was most effective in the xenoestrogen-dependent induction of reporter activity. The extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. The data in this research demonstrated that the yeast detection system using steroid hormone receptor and co-activator is a useful tool for identifying chemicals that interact with steroid receptors.s.

• The Journal of Korean Physical Therapy
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• 제14권1호
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• pp.187-195
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• 2002

This study was performed to assess the efficacy of Activator for the patient with chronic neck pain. Mail, 26 years old, is treated with Activator, which was applied for 15 minutes every two days during the 4 weeks. The results of this study are as follows: 1. The treated case compared with the previous case showed that R.O.M particularly increased in flexion and extension. 2. The atlas/skull angle compared with the previous case showed that the angle increased from $0.8^{\circ}$ to $1.5^{\circ}$. In the atlas/skull, normal angle is $5.6^{\circ}$. 3. The angle of cervical curve lordosis compared with the previous case showed that the angle increased from $38.3^{\circ}$ to $48.1^{\circ}$. In the healthy spine, the angle is $42.0^{\circ}$. 4. The anterior curvature compared with the previous case showed that curve radii decreased from 7,220mm to 200mm. In the spine, normal curvature is 165mm. 5. Rush Jackson's angle compared with the previous case showed that the angle increased from $14.8^{\circ}$ to $29.1^{\circ}$. In the healthy spine, normal angle is $39.9^{\circ}$. We can not find significant level for Activator, but it is considered that activator method of chiropractic technique is effective.

• Nutrition Research and Practice
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• 제14권5호
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• pp.463-477
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• 2020

BACKGROUND/OBJECTIVES: Many studies have suggested that Rhus verniciflua Stokes (RVS) and its extract are anticancer agents. However, RVS had limited use because it contains urushiol, an allergenic toxin. By improving an existing allergen-removal extraction method, we developed a new allergen-free Rhus verniciflua Stokes extract (RVSE) with higher flavonoid content. In this study, we examined whether RVSE inhibits the ability of AGS gastric cancer cells to migrate and invade. MATERIALS/METHODS: The flavonoids content of RVSE was analyzed by HPLC. The effects of RVSE on migration and invasion in AGS cells were analyzed by each assay kit. Matrix metalloproteinase (MMP)-9, plasminogen activator inhibitor-1 (PAI-1) and urokinase-type plasminogen activator (uPA) protein expression was analyzed by protein antibody array. The Phosphorylation of signal transducer and activator of transcription (STAT) 3 were assayed by Western blot analysis. RESULTS: RVSE treatment with 0-100 ㎍/mL dose-dependently reduced the ability of AGS cells to migrate and invade. Notably, treatment with RVSE strongly inhibited the expression of MMP-9 and uPA and the phosphorylation of STAT3. In contrast, RVSE treatment dramatically increased the expression of PAI-1. These results indicate that the inhibition of MMP-9 and uPA expression and STAT3 phosphorylation and the stimulation of PAI-1 expression contributed to the decreased migration and invasion of AGS cells treated with RVSE. CONCLUSIONS: These results suggest that RVSE may be used as a natural herbal agent to reduce gastric cancer metastasis.

• Journal of Korean Neurosurgical Society
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• 제37권4호
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• pp.287-292
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• 2005

Objective: Recent clinical studies have demonstrated that intracisternal administration of recombinant tissue plasminogen activator(rt-PA) can facilitate the normal clearing of blood from the subarachnoid space. Urokinase, a first generation fibrinolytic agent, has been used to liquify such clots with some success. Therefore, recombinant tissue plasminogen activator, a second generation fibrinolytic drug that may be safer and more effective, is studied to evaluate its dosage to lyse clots in vitro and reactivity in the brain parenchyme. Methods: Intracerebral hematomas were created by stereotactically injecting 2ml of clotted autogenous blood into the brain parenchyme of total 28 anesthetized adult cats (weighting 3.8 to 4.1 kg). The control animals (group A) received 1 ml of normal saline injected into the clots and the experimental animals received each 0.1 mg of rt-PA (group B), 0.5mg of rt-PA (group C) and 1 mg of rt-PA (group D) at 6 hours after the clot injection. Results: 1. The amount of remained clots after lysing the hematomas were as follows: $1.80{\pm}0.17ml$ in group A, $1.65{\pm}0.23ml$ in group B, $0.61{\pm}0.37ml$ in group C and $0.52{\pm}0.34$ in group D. The result indicated that hematomas in rt-PA treated groups (C & D) were lysed better than the control group. 2. At least 0.5mg of rt-PA should be required for the lysis of 2ml of hematomas. 3. Light microscopic examination revealed no histological evidence of hemorrhage in tissue sections from each brain. Conclusion: Recombinant tissue plasminogen activator may be safely and effectively employed for the lysis of intracerebral hematomas in animal model.

• BMB Reports
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• 제35권4호
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• pp.371-376
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• 2002

The receptor activator of nuclear factor kappa B (RANK) is a member of the tumor necrosis factor (TNF) receptor superfamily. It plays a critical role in osteoclast differentiaion, lymph node organogenesis, and mammary gland development. The stimulation of RANK causes the activation of transcription factors NF-${\kappa}B$ and activator protein 1 (AP1), and the mitogen activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK). In the signal transduction of RANK, the recruitment of the adaptor molecules, TNF receptor-associated factors (TRAFs), is and initial cytoplasmic event. Recently, the association of the MAPK kinase kinase, transforming growth factor-$\beta$-activated kinase 1 (TAK1), with TRAF6 was shown to mediate the IL-1 signaling to NF-${\kappa}B$ and JNK. We investigated whether or not TAK1 plays a role in RANK signaling. A dominant-negative form of TAK1 was discovered to abolish the RANK-induced activation of AP1 and JNK. The AP1 activation by TRAF2, TRAF5, and TRAF6 was also greatly suppressed by the dominant-negative TAK1. the inhibitory effect of the TAK1 mutant on RANK-and TRAF-induced NF-${\kappa}B$ activation was also observed, but less efficiently. Our findings indicate that TAK1 is involved in the MAPK cascade and NF-${\kappa}B$ pathway that is activated by RANK.

• 대한소아치과학회지
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• 제28권3호
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• pp.496-503
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• 2001

골격성 II급 1류 부정교합을 가지는 아동에서 상악골의 과도한 성장은 흔히 전후방적 요소 뿐 아니라 수직적 요소도 가지고 있는데, 그 이유는 상악골이 전하방으로 성장, 이동할 경우 하악골이 후하방 회전하기 때문이며, 하악골의 성장이 전방으로 이루어지는 것을 방해하게 된다. 따라서 이 경우 치료목표는 하악골이 더욱 돌출 되어 상악골과 정상적인 관계를 가지도록 성장하는 동안 상악골의 성장을 억제하는 것이며, 이를 위해 구외력을 적용하는 것이 현명한 방법으로 보고되고 있다. 조선대학교 소아치과에 내원한 골격성 II급 1류 부정교합 환자에서 C II activator와 headgear를 이용한 구외력을 적용후 양호한 결과를 얻었기에 보고하는 바이다.

• 대한치과교정학회지
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• 제33권4호
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• pp.247-257
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• 2003

골격성 II급 1류 부정교합을 치료하는 방법으로 Activator, FR(Functional regulator), Headgear, Herbst 장치, Twin-block등의 많은 장치가 사용되어진다. 이러한 장치를 사용할 때 기대하는 가장 바람직 한 효과는 상악골의 수평적, 수직적 성장의 억제와, 하악골의 성장이 촉진되어 바람직한 골격적 관계를 가지게 되고 이러한 토대 위에 치아들이 적절하게 배열되는 것일 것이다. Teuscher장치는 Headgear의 상악골의 전하방 성장억제작용과 Activator의 하악골 성장촉진 작용을 동시에 가질 수 있도록 고안된 장치로서 실제 임상 중에 이러한 효과가 잘 구현될 수 있는지를 평가하기 위하여 성장기 남녀 각각9명(평균연령 남자 8세 11개월, 여자 9세 4개월)을 대상으로 Teuscher appliance를 평균 남아는 평균10개월, 여 아는 평균 11개월간 사용하였으며 이를 대조군과 비교 연구를 시행하였고 그 결과는 다음과 같았다. 1. 상악골의 전방 성장을 효과적으로 억제하여 주었다. 2. 상악 dentoalveolar complex의 전하방 성장을 억제하여 주었다. 3. 하악골의 전방 성장 촉진을 관찰하지 못하였다. 4. Overjet, overbite, molar key 개선에 효과적이었다. 5. 장치의 사용으로 상순의 돌출도의 개선과 안모가 향상되었다.

• 공업화학
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• 제19권2호
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• pp.168-172
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• 2008

오염물질을 세탁하는 과정에 있어서 보다 효과적으로 오염물질을 제거하고 표백효과를 부여하기 위해서는 기존의 계면활성제만으로는 한계가 있어 추가적으로 표백과정을 거친다. 본 논문에서는 가장 일반적으로 사용되는 표백활성화제인 TAED (tetraacetylethylenediamine)와 신규로 합성한 표백활성화제(OBA, organic bleach activator, decanoyloxyethoxycarbonyloxybenzenesulfonate)를 비교 평가하였다. 합성한 OBA의 안정성을 개선하고자 제오라이트와 폴리옥시에틸렌 글리콜을 사용하여 코팅처리 한 결과 우수한 안정성을 보였다. OBA는 저온($20^{\circ}C$)에서도 우수한 표백효과를 나타냈으며 TAED에 비하여 미생물 분해율이 88%인 물질로 OECD기준(60%) 대비 쉽게 생분해되었다. 세탁과정 중에서 무좀균이나 황색포도상구균에 대해서도 우수한 살균력을 나타내었다.

• 한국재료학회지
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• 제30권1호
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• pp.38-43
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• 2020

Sand casting 3D printing uses a binder jetting method to produce a mold having complicated shape by spraying a binder on sand coated with activator. Appropriate heat treatment process in sand mold fabrication can increase the degree of polymerization to improve flexural strength. However, long heat treatment of over 24 hours decreases flexural strength and reliability due to chemical bond decomposition through thermal degradation. The main role of the activator is to control the reaction rate between the polymer chains. As a result, when the activator composition is increased from 0.15 wt% to 0.25 wt%, the flexural strength is increased by 218 N/㎠. However, excess activator (0.40 wt%) has been shown to decrease reliability without increasing flexural strength. The main role of the binder is to control the flexural strength of the specimen. As the binder composition is increased from 2.00 wt% to 4.00 wt%, the flexural strength increases to about 255 N/㎠, indicating the maximum flexural strength increase. Finally, the reliability of the flexural strength of the fabricated specimens is evaluated by a Weibull plot. Weibull modulus calculations are used to evaluate the flexural strength reliability of the specimens, and maximum reliability value of 11.7 is obtained at 0.20 wt% activator composition. Therefore, it is confirmed that this composition has maximum flexural strength reliability.

• 대한소아치과학회지
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• 제29권3호
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• pp.313-317
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• 2002

어린이는 성인과 다른 악관절 구조를 가지고 있으며 이로 말미암아 견고한 중심교합위를 얻지 못하거나 이원교합(dual bite)의 경우가 많다. 이러한 경향이, 편측저작 등의 치열변화를 초래할 수 있는 습관과 합쳐져 골격적 변화를 초래하게 될 경우, 안면 비대칭이 유도될 수 있다. 이러한 경우의 치료로서 교합유도를 통한 관절의 안정화와 그에 맞는 교합의 재형성을 통한 관절 및 교합의 안정화가 필요하다. 안면 비대칭을 주소로 내원한 5세 어린이에서 편측저작 습관으로 말미암은 우측 편측 구치부 절단교합과 2.5mm의 정중선 불일치가 있었다. 저자들은 activator를 이용하여 악관절 및 교합을 정상위치로 유도한 후 유구치를 주조금관으로 수복하여 양호한 결과를 얻었다.