• Animal Bioscience
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• 제36권2호
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• pp.175-190
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• 2023

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

• 인간행동과 음악연구
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• 제14권2호
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• pp.19-43
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• 2017

본 연구는 만 4-5세의 자폐범주성장애 아동 4명을 대상으로 음악 조건과 비음악 조건의 단계적 상호 주의 중재를 교대로 실시하여 공동주의 기술 향상에 미치는 중재 효과를 비교하고자 하였다. 이를 위해 단일대상연구의 한 가지 방법인 교대중재설계를 사용하였고, 음악 조건과 비음악 조건의 중재를 사전에 결정된 무작위 순서에 따라 회기 내 교대로 실시하였다. 총 23회기의 전체 프로그램은 기초선(3회기), 중재(15회기), 유지(5회기)의 절차로 이루어졌다. 음악 조건에서는 구조화된 공동 악기 연주, 음악적 신호(cue)에 따른 주의전환, 상호적 악기연주가 이루어졌고, 비음악 조건에서는 조립장난감을 이용한 공동 놀이, 언어적 신호에 따른 주의전환, 장난감 주고받기가 이루어졌다. 중재 내 나타난 공동주의 행동 변화는 공동주의 반응행동과 얼굴 쳐다보기, 공동 활동에 참여하기의 발생 빈도로 측정되었고, 중재 사전 사후에는 초기 사회-의사소통 평가를 실시하였다. 연구 결과, 두가지 조건 모두에서 공동주의 행동이 증가하는 추이를 보였으나, 음악 조건이 비음악 조건에 비해 증가하는 폭이 크고 더 높은 수준에서 유지되는 것을 확인할 수 있었다. 본 연구에서 도출된 결과는 구조화된 음악적 환경 안에서, 음악을 통해 사회적 정보를 처리하고 주의를 조절하는 중재가 자폐범주성장애 아동의 공동주의 행동을 향상시키는 데 효과적임을 시사한다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 1995년도 추계학술대회 초록
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• pp.11-34
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• 1995

Growth hormone (GH) plays a key role in regulating postnatal growth and can stimulate growth of animals by acting directly on specific receptors on the plasma membrane of tissues or indirectly through stimulating insulin-like growth factor (IGF)-I synthesis and secretion by the liver and other tissues. IGF-I and IGF-Ⅱ are polypeptides with structural similarity with proinsulin that stimulate cell proliferation by endocrine, paracrine and autocrine mechanisms. The initial event in the metabolic action of IGFs on target cells appears to be their binding to specific receptors on the plasma membrane. Current evidence indicates that the mitogenic actions of both IGFs are mediated primarily by binding to the type I IGF receptors, and that IGF action is also mediated by interactions with IGF-binding proteins (IGFBPs). Six distinct IGFBPs have been identified that are characterized by cell-specific interaction, transcriptional and post-translational regulation by many different effectors, and the ability to either potentiate or inhibit IGF actions. Nutritional deficiencies can have their devastating consequence during growth. Although IGF-I is the major mediator of GH's action on somatic growth, nutritional status of an organism is a critical regulator of IGF-I and IGFBPs. Various nutrient deficiencies result in decreased serum IGF-I levels and altered IGFBP levels, but the blood levels of GH are generally unchanged or elevated in malnutrition. Effects of protein, energy, vitamin C and D, and zinc on serum IGF and IGFBP levels and tissue mRNA levels were reviewed in the text. Multiple factors are involved in the regulation of intestinal epithelial cell growth and differentiation. Among these factors the nutritional status of individuals is the most important. The intestinal epithelium is an important site for mitogenic action of the IGFs in vivo, with exogenous IGF-I stimulating mucosal hyperplasia. Therefore, the IGF system appears to provide and important mechanism linking nutrition and the proliferation of intestinal epithelial cells. In order to study the detailed mechanisms by which intestinal mucosa is regulated, we have utilized IEC-6 cells, an intestinal epithelial cell line and Caco-2 cells, a human colon adenocarcinoma cell line. Like intestinal crypt cells analyzed in vivo or freshly isolated intestinal epithelial cells, IEC-6 cells and Caco-2 cells possess abundant quatities of both type Ⅰ and type Ⅱ IGF receptors. Exogenous IGFs stimulate, whereas addition of IGFBP-2 inhibits IEC-6 cell proliferation. To investigate whether endogenously secreted IGFBP-2 inhibit proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA anti-sense expression construct. IEC-6 cells transfected with anti-sense IGFBP-2 protein in medium. These cells grew at a rate faster than the control cells indicating that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs. IEC-6 cells express many characteristics of enterocyte, but do not undergo differentiation. On the other hand, Caco-2 cells undergo a spontaneous enterocyte differentiation. On the other hand, Caco-2 cells undergo a spontaneous enterocyte differentiation after reaching confluency. We have demonstrated that Caco-2 cells produce IGF-Ⅱ, IGFBP-2, IGFBP-3, and an as yet unidentified 31,000 Mr IGFBP, and that both mRNA and peptide secretion of IGFBP-2 and IGFBP-3 increased, but IGFBP-4 mRNA and protein secretion decreased after the cells reached confluency. These changes occurred in parallel to and were coincident with differentiation of the cells, as measured by expression of sucrase-isomaltase. In addition, Caco-2 cell clones forced to overexpress IGFBP-4 by transfection with a rat IGFBP-4 cDNA construct exhibited a significantly slower growth rate under serum-free conditions and had increased expression of sucrase-isomaltase compared with vector control cells. These results indicate that IGFBP-4 inhibits proliferation and stimulates differentiation of Caco-2 cells, probably by inhibiting the mitogenic actions of IGFs.

• 한국전자거래학회지
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• 제13권3호
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• pp.1-20
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• 2008

오늘날 컴퓨팅 환경은 과거에 비해 매우 분산되어 있으며 다양한 시스템과 연결되고 통합되는 동적인 구성을 필요로 한다. 대부분의 기업들은 이를 위해 객체지향적인 메시지 송수신 체계인 MOM(Message Oriented Middleware) 시스템을 사용하여 원격의 시스템과의 통신채널을 구성하고 송수신 정보를 XML 메시지로 주고받고 있다. 그러나 대부분의 MOM 시스템들은 송수신 메시지로 사용되는 XML을 처리함에 있어 XML 메시지의 동적 변환 및 라우팅을 지원하지 않아 기업 시스템의 효율성과 유지보수성을 저하 시키고 있다. 이에 본 논문에서는 가장 널리 사용되고 있는 MOM 시스템인 JMS를 기반으로 동적 XML 메시지 변환 및 라우팅을 지원하기 위한 룰 기반의 라우팅 시스템인 RMRS(Rule-based Message Routing System)를 제안한다. RMRS는 룰 기반 이벤트 처리를 위해 사용되는 ECA(Event- Condition-Action)룰을 확장하여 분산 시스템 구현 시에 빈번하게 발생하는 라우팅 처리를 XML 변환과 함께 동적으로 처리할 수 있도록 한다. 이것은 내부적으로 메시지가 라우팅 되기 전에 수신한 메시지의 성격에 따라 XML 메시지의 변환을 수행할 수 있도록 설계되어 있어, 이를 바탕으로 MOM 시스템을 구축할 경우, XML 메시지의 동적 변환 및 라우팅을 효과적으로 구현할 수 있다.

• 기술혁신학회지
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• 제20권3호
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• pp.732-753
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• 2017

최근 들어 바이오 제약 의료 분야의 기술가치평가를 수행할 때 미래에 투입될 사업화 소요기간 및 비용을 고려하여 기술가치를 산정하는 경우가 증가하고 있다. 기존의 현금흐름할인법(DCF법)은 연속된 투자에 대한 고려를 못하거나 기술적용 제품의 상용화 투입비용에 대한 확률적인 속성을 반영하지 못하는 등 한계점을 지니고 있다. 그러나 기술과 투자의 가치는 기회가치로 보고 자원배분을 위한 의사결정 정보를 감안해야 하므로, 실물옵션의 개념을 적용하는 것이 바람직하다고 여겨지며, 흔히 기업가치평가 수행시 적용하는 주가의 변동성(volatility) 개념을 대상기술에 대한 비즈니스 모델 특성이 반영되도록 하기 위해 '주가의 연속성(상대적 미세한 변화)' 및 '양(+)의 조건'을 고려해야 한다. 따라서 많은 문헌에서 연구된 바와 같이, 실물옵션 기반의 기술가치 산정을 위한 블랙-숄즈 모형에서 변동성과 기초자산가치, 그리고 사업화비용 간의 관계를 살펴볼 필요가 있다. 본 연구는 옵션가격결정모형(Option Pricing Model)에서 불확실성을 반영한 기초자산의 현재가치와 사업화비용의 현재가치 비율이 특정 임계조건 하에서 '옵션행사 포기(NAT; no action taken)' 영역으로 구분되는 지를 수학적으로 도출하고 관찰변수(입력값)에 따른 옵션가치 산출로직을 제시함으로써 정교화된 실물옵션 모형을 제공할 수 있을 것으로 기대된다.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 1999년도 추계학술발표회 초록집
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• pp.15-15
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• 1999

In chip plating, several parameters must be taken into consideration. Current density, solution concentration, pH, solution temperature, components volume, chip and media ratio, barrel geometrical shape were most likely found to have an effect to the process yields. The 3 types of barrels utilized in chip plating industry are the conventional rotating barrel. vibrational barrel (vibarrel), and the centrifugal type. Conventional rotating barrel is a close type and is commonly used. The components inside the barrel are circulated by the barrel's rotation at a horizontal axis. Process yield has known to have higher thickness deviation. The vibrational barrel is an open type which offers a wide exposure to electrolyte resulting to a stable thickness deviation. It rotates in a vertical axis coupled with multi-vibration action to facilitate mixed up and easy transportation of components, The centrifugal barrel has its plated work centrifugally compacted against the cathode ring for superior electrical contact with simultaneous rotary motion. This experiment has determined the effect of barrel vibration intensity to the plating thickness distribution. The procedures carried out in the experiment involved the overall plating process., cleaning, rinse, Nickel plating, Tin-Lead plating. Plating time was adjusted to meet the required specification. All other parameters were maintained constant. Two trials were performed to confirm the consistency of the result. The thickness data of the experiment conducted showed that the average mean value obtained from higher vibrational intensity is nearer to the standard mean. The distribution curve shown has a narrower specification limits and it has a reduced variation around the target value, Generally, intensity control in vi-barrel facilitates mixed up and easy transportation of components, However, it is desirable to maintain an optimum vibration intensity to prevent solution intrusion into the chips' internal electrode. A cathodic reaction can occur in the interface of the external and internal electrode. $2HD{\;}+{\;}e{\;}{\rightarrow}20H{\;}+{\;}H_2$ Hydrogen can penetrate into the body and create pressure which can cause cracks. At high intensity, the chip's motion becomes stronger, its contact between each other is delayed and so plating action is being controlled. However, the strong impact created by its collision can damage the external electrode's structure thereby resulting to bad plating condition. 1 lot of chip was divided into two equal partion. Each portion was loaded to the same barrel one after the other. Nickel plating and tin-lead plating was performed in the same station. Portion A maintained the normal barrel vibration intensity and portion B vibration intensity was increased two steps higher. All other parameters, current, solution condition were maintained constant. Generally, plating method find procedures were carried out in a best way to maintained the best plating condition. After plating, samples were taken out from each portion. molded and polished. Plating thickness was investigated for both. To check consistency of results. 2nd trial was done now using different lot of another characteristics.

• 동의생리병리학회지
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• 제19권5호
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• pp.1391-1398
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• 2005

The anti-thrombic properties of the oriental herbal medicine Daejowhan(DJW, 大造丸) which consists of 11 kinds of herbs (indicated as ratio) of Rehmanniae Radix 24%, Hominis Placenta 5%, Testudinis Carapax 9%, Eucommiae Cortex 9%, Asparagi Radix 9%, Phellodendri Cortex 9%, Achyranthis Radix 7%, Liriopis Tuber 7%, Angelicae Sinensis Radix 7%, Ginseng Radix 5% and Schizandrae Fructus 3% were investigated. The water extracts from DJW inhibited Platelet-activating factor(PAF) induced platelet aggregation. DJW was extracted with methanol and further fractionated by ethylacetate. A 70% methanol extract showed a strong inhibition against PAF-induced aggregation in vitro and in vivo assays. The ethylacetate soluble fraction was shown to have inhibitory effect on PAF-induced platelet aggregation in vitro assay. The ethylacetate soluble fraction specially protected against the lethality of PAF, while verapamil did not afford any protection. These results indicate that the water extracts and alcoholic-fractions inhibit the action of PAF in vivo by an antagonistic effect on PAF, so that it may be useful in treating disorders caused by PAF, such as acute allergy, inflammation, asthma, gastrointestinal ulceration, toxic shock and so forth. DJW was investigated regarding its assumed anti-thrombic action on human platelets which was deduced from its ability to suppress Arachidonic acid(AA)-induced aggregation, exocytosis of ATP, and inhibition of Cyclooxygenase(COX) and Thromboxane synthase(TXS) activity. The latter two effects were estimated from the generation of Prostaglandin $E_2(PGE_2)$ and Thromboxane $A_2(TXA_2)$ respectively. Exogenously applied AA ($100{\mu}mol/{\ell}$) provoked a $89\%$ aggregation of platelets, the release of 14 pmol ATP, and the formation of either 225 pg $TXA_2$ or 45 pg $PGE_2$, each parameter being related to 106 platelets. An application of DJW 5 min before AA dose-dependently diminished aggregation, ATP-release and the synthesis of $TXA_2$ and $PGE_2$ with $IC_{50}$ values of 74, 108, 65, $72{\mu}g/m{\ell}$, respectively. The similarity of the $IC_{50}$ values suggest an inhibition of COX by DJW as primary target, thus suppressing the generation of $TXA_2$ which induces aggregation of platelets and exocytosis of ATP by its binding on $TXA_2$-receptors.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.225-236
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• 2013

Among several bacteria examined, an antibacterial-producing Lactobacillus strain with probiotic characteristics was selected and identified based on 16S rRNA gene sequencing. Subsequent purification and mode of action of the antibacterial compounds on target cells including E. coli were investigated. Maximum production of the antibacterial compound was recorded at 18 h incubation at $30^{\circ}C$. Interestingly, antibacterial activity remained unchanged after heating at $121^{\circ}C$ for 45 min, 24 h storage in temperature range of $70^{\circ}C$ to room temperature, and 15 min exposure to UV light, and it was stable in the pH of range 2-10. The active compounds were inactivated by proteolytic enzymes, indicating their proteinaceous nature, and, therefore, referred to as bacteriocin-like inhibitory substances. Isolation and partial purification of the effective agent was done by performing ammonium sulfate precipitation and gel filtration chromatography. The molecular mass of the GFC-purified active compound (~3 kDa) was determined by Tris-Tricine SDS-PAGE. To predict the mechanisms of action, transmission electron microscopy (TEM) analysis of ultrathin sections of E. coli before and after antibacterial treatment was carried out. TEM analysis of antibacterial compounds-treated E. coli demonstrated that the completely altered bacteria appear much darker compared with the less altered bacteria, suggesting a change in the cytoplasmic composition. There were also some membrane-bound convoluted structures visible within the completely altered bacteria, which could be attributed to the response of the E. coli to the treatment with the antibacterial compound. According to the in vivo experiments oral administration of L. plantarum HKN01 resulted in recovery of infected BALB/c mice with Salmonella enterica ser. Typhimurium.

• 한국지하수토양환경학회지:지하수토양환경
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• 제16권1호
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• pp.32-41
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• 2011

By using a newly developed Korean risk-based corrective action (K-RBCA) software (K-RBCA) and the RBCA Tool Kit, risk assessment was performed on a site that was contaminated with aromatic hydrocarbons and heavy metals. Eight chemicals including benzene, ethylbenzene, xylenes, naphthalene, benz(a) anthracene, benzo(b) fluoranthene, benzo(a) pyrene, and arsenic that exceeded the US EPA Soil Screening Level were chosen as the target pollutants. A conceptual site model was constructed based on the site-specific effective exposure pathways. According to the RBCA Tool Kit the carcinogenic risk of arsenic was larger than $10^{-6}$, which is the generally acceptable carcinogenic risk level. The K-RBCA estimated the same level of carcinogenic risk for arsenic. With the RBCA Tool Kit, the carcinogenic risk of benzo(a) pyrene was estimated to be about $1.3{\times}10^{-6}$. However, with the K-RBCA benzo(a) pyrene did not exhibit any risk. The inconsistency between the softwares was attributed to the different fundamental settings (i.e., medium division) between the two softwares. While the K-RBCA divides medium into surface soil, subsurface soil, and groundwater, the RBCA Tool Kit divides medium into only soil and groundwater. These differences lead to the different exposure pathways used by the two softwares. The K-RBCA considers the exposure pathways in surface soil and subsurface soil separately to estimate risk, however, the RBCA Tool Kit considers the surface soil and subsurface soil as one and uses the integrated exposure pathways to estimate risk. Thus the resulting risk is higher when the RBCA Tool Kit is used than when the K-RBCA is used. The results from this study show that there is no significant difference in the risks estimated by the two softwares, thus, it is reasonable to use the K-RBCA we developed in risk assessment of soil and groundwater. In addition, the present study demonstrates that the assessor should be familiar with the characteristics of a contaminated site and the assumptions used by a risk assessment software when carrying out risk assessment.

• 감성과학
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• 제12권3호
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• pp.325-330
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• 2009

신기술의 지속적인 적용으로 휴대폰의 사용 난이도가 높아짐에 따라 user interface의 중요성이 강조되고 있다. User interface 개발 프로세스의 복잡도와 이에 따르는 로드 또한 지속적으로 높아지고 있다. 본 논문에서는 체계화된 휴대폰 user interface의 개발 방법론에 대하여 논의하였다. 기존 휴대폰 개발 프로세스를 검토하고, 이에 따르는 개발상의 이슈사항을 살피고, 4단계의 가이드라인 적용을 통한 user interface 개발 프로세스를 제안하고 있다. 기존 유저 인터페이스와 관련한 연구들은 대부분 개발 대상에 대해 집중되어 있으나 좋은 user interface는 좋은 UI개발 프로세스를 통하여 실현될 수 있으며, 때문에 UI 매니지먼트 레벨의 논의는 제품 UI 개발과는 별도로 다루어질 필요가 있다. 본 논문에서 제시된 4단계 가이드라인은 휴대폰의 UI 개발뿐만 아니라 타제품 및 서비스의 UI개발에도 응용될 수 있을 것으로 사료된다.