• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.151-154
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• 2000

The effect of silkworm hemolymph on insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity. Silkworm hemolymph also inhibits apoptosis induced by actinomycin D, the RNA synthesis inhibitor. In this study, a flow cytometry was used for the quantitative analysis of apoptosis inhibition by silkworm hemolymph.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.268-268
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• 1994

포자의 전자현미경사진과 mycelium의 광학현미경사진을 찍어 strain V-8 균주의 형태학적인 특성을 조사하였을 때 방선균 계열인 streptomyces로 확인되었다. 이 균주의 배양액은 노란색을 띠고 있고 이 노란색은 430 nm에서 특징적인 흡광을 보였다. 이 물질은 유기 용매로 추출되었다. 이 물질의 자외선 흡광스펙트럼의 특징을 기존에 보고된 화합물과 비교하여 이 물질이 actinomycin계열에 속함을 발견하였다. 이 물질의 FAB mass spectrum에서 각 분획들의 주성분들의 분자의 질량 + H 이온이 1290과 1292에서 각각 관찰되었다. 이 화합물들은 분자량이 1258인 actinomycin D와는 상이한 구조를 소유하고 있는 것으로 확인되었다. 각 분획 성분들의 proton 및 carbon HIR spectra를 얻어 기존에 알려진 화합물의 자료와 비교하였을 때 actinomycin계열에 속하는 신물질로 생각되었다. 지금까지 actinomycin 계열의 항생물질이 adenosine deaminase의 효소 활성을 저해한다는 사실은 보고된바 없다. 본 연구자는 분리한 2개의 화합물에 대하여 구조 연구를 계속하고 있다.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.1
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• pp.387-390
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• 2012

A combination of 5-fluorouracil plus actinomycin D (5FU plus Act D) is the regimen that has been commonly administered to Chinese and Japanese gestational trophoblastic neoplasia patients as the first or second line of treatment with an excellent outcome. However, the efficacy of this regimen in a salvage setting was unclear. To evaluate the efficacy and safety of the 5 FU plus Act D regimen utilized in this condition, all GTN patients resistant to at least three previous chemotherapy regimens who received the 5 FU plus Act D regimen between August 2009 and January 2011 at Chiang Mai University Hospital were reviewed. There were five cases who met the criteria. Four of those patients were in FIGO stage III to IV with a WHO scoring of more than 12. The median number of cycles for each patient was two and only one case achieved remission while four of the cases were unresponsive. The toxicity was evaluated in 12 cycles. Common complications were uncomplicated myelosuppression and mucositis. In conclusion, this regimen revealed modest efficacy in a salvage setting with manageable toxicity.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.432-434
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• 1989

• Journal of Life Science
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• v.16 no.4
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• pp.598-604
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• 2006

The $Ca^{2+}-activated$ neutral protease calpain induced proteolysis has been suggested to play a role in certain cell growth regulatory proteins. Cyclin proteolysis is essential for cell cycle progression. D-type cyclins, which form an assembly with cyclin-dependent kinases (cdk4 and cdk6), are synthesized earlier in G1 of the cell cycle and seem to be induced in response to external signals that promote entry into the cell cycle. Here we show that cyclin D3 protein levels are regulated at the posttranscriptional level by calpain protease. Treatment of human breast carcinoma MDA-MB-231 cells with lovastatin and actinomycin D resulted in a loss of cyclin D3 protein that was completely reversible by the peptide aldehyde calpain inhibitor, LLnL. The specific inhibitor of the 26S proteasome, lactacystin, the lysosome inhibitors, ammonium chloride and chloroquine, and the serine protease inhibitor, phenylmethylsulfonylfluoride (PMSF), did not block the degradation of cyclin D3 by lovastatin and actinomycin D. Results of in vitro degradation of cyclin D3 by purified calpain showed that cyclin D3 protein is degraded in a $Ca^{2+}-dependent$ manner, and the half-life of cyclin D3 protein was dramatically increased in LLnL treated cells. These data suggested that cyclin D3 protein is regulated by the $Ca^{2+}-activated$ protease calpain.

• Archives of Pharmacal Research
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• v.17 no.6
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• pp.424-427
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• 1994

An acinomycin complex isolated from culture broth of a soil microorganism, SNUS 9305-011 has been examined by High performance liquid chromatography (HPLC). From the analysis of the fractions obtained by column chromatography of the ethyl acetate extract, three actinomycin components are confimed . The HPLC analysis is carried out with a CN-bonded nucleosil column. Comparison of the retention times of the components with those of actinomycin D, C complex, $X_{o{\beta}$, and V and suggests that they are different actinomycins. FBA mass spectra fo the coponents also shows different molecular ions from those of standards and other reported actionbmycins. The present work has demonstrated that actinomycin components can be separated by a CN-bonded HPLC column, and that ocmparison of their HPLC chormatograms with authentic smaples and information on their molecular ions can be successfully employed for indentification of actionmycins.

• Journal of Environmental Health Sciences
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• v.17 no.2
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• pp.121-126
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• 1991

To clarify a cause of increased serum level of acid phosphatase in CCl$_{4}$-treated rats, the acid phosphatase activity of liver was compared with that serum. Concomitantly, the serum and liver acid phosphatase activity of CCl$_{4}$-treated rats were compared with that of CCl$_{4}$-treated rats pretreated with prednisolone or actinomycin D. In CCl$_{4}$-treated rats, the activity of serum acid phosphatase was significiantly increased whereas that of liver acid phosphatase was rather slightly decreased. the pretreatment of prednisolone led to the decreased activity of serum and liver acid phosphatase in CCl$_{4}$-treated rats. But the pretreatment of actinomycin D rather increased the activity of liver and serum enzyme. In conclusion, it is likely the increased activity of serum acid phosphatase is based on the excess leaking of acid phosphatase into blood by the increased membrane permeability of both liver cell and lysosome in it.

• Microbiology and Biotechnology Letters
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• v.22 no.2
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• pp.169-174
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• 1994

Antitumor antibiotic HS-1 was purified from the culture broth of a streptomyces floridae SHS-1372 which had been isolated from soil, by solvent extraction, silica gel column chromatography and gel filtration. It was confirmed that HS-1 was active against gram positive bacteria and cancer cells(K562, P388, MCF-7, HT-29). Through the analysis of UV spectrum, melting point, IR spectrum, FAB-MS, $_{1}$H-NMR, $_{13}$C-NMR, 2D-NMR spectra, HS-1 could be identified sa the actinomycin X$_{2}$ antibiotics containing actinocine chromophore and peptides consisted of threonine, proline, methylvaline, sarcosine, D-valine and 4-ketoproline.

• Journal of Environmental Health Sciences
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• v.16 no.2
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• pp.121-126
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• 1990

Liver and serum xanthine oxidase(XOD) activity were determined in rats treated with DL-ethionine. Concomitantly, the enzyme activity was compared with that of rats treated with CCl$_{4}$, actinomycin D and those fed a protein depleted diet. The activity of XOD in liver was inhibited by injection of ethionine to the rats. But, there were no differences in activity of serum XOD between control group and ethionine-treated rats. And the pattern of changes in enzyme activity of serum and liver in ethionine-treated rats, was similar with actinomycin D treated rats or those fed a protein depleted diet. On the other hand, the activity of XOD was rather elevated both in serum and liver by injection of CCl$_{4}$ to rats.

• Journal of Food Hygiene and Safety
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• v.11 no.4
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• pp.227-234
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• 1996

This study was designed to characterize endotoxin-induced prostaglandin production in primary cultured rat vascular smooth muscle cells (VSMC). The time course for prostaglandin synthesis in lipopolysaccharide (LPS)-stimulated VSMC showed that the maximum production was reached in 12 hours. LPS induced prostaglandin H2 synthase (PGHS) activity in VSMC and the time course profile in the changes of PGHS activity paralleled that of total prostaglandin production. Differential treatment showed that 4 hours' exposure to LPS was enough for the maximum effect on the prostaglandin production and this effect was completely inhibited by the co-treatment of actinomycin D, a transcription inhibitor. These results suggest that LPS effect might be determined within 4 hours. Actinomycin D increased PGHS activity without affecting prostaglandin production if added 4 hours after LPS treatment. On the other hand, cyclogeximide, a translation inhibitor, augmented LPS-induced prostaglandin production if treated during first four hours, but it inhibited LPS-induced PGHS activity regardless of treatment schedule. These results suggest the existence of multiple regulating mechanisms in the LPS-induced prostaglandin synthesis.