• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.846-852
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• 2003

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.139-140
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• 1993

토양으로부터 Actinomycetes 분리: Actinomycetes는 항생물질이나 항암물질을 많이 생산하는 중요한 균주로 의학계나 농업분야에 널리 이용되고 있다. Adenosine deaminase 저해제를 생산하는 균주를 찾기 위해 토양으로부터 actinomycetes를 분리하였다 Suluble-starch-casein배지와 AS-1배지를 이용하였는데 이들은 탄소원으로 용해성 전분을 공통적으로 함유하고 있다. 나무밑이나 산, 언덕등의 건조한 토양이나 두엄이나, 두엄밑의 토양을 탄산칼슘을 처리하여 주었을 때 actinomycetes가 많이 생장하는 것으로 알려져 있으므로 이러한 흙들을 채취하여 actinomycetes 분리에 이용하였다.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.191-202
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• 1998

About 300 actinomycetes were isolated from two forest and one sea-shore soil and tested for inhibitory effects on mycelial growth of six plant pathogenic fungi Magnaporthe grisea, Alternaria mali, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f. sp. cucumerinum, and Rhizoctonia solani. Among 300 actinomycetes tested, only 16 actinomycetes showed the antifungal activity against the test fungi. Isolate NH 50 was selected for production and purification of antifungal antibiotic substances. Actinomycete isolate NH 50 displayed the broad antifungal spectra against 11 plant pathogenic fungi. To identify actinomycete isolate NH 50, cultural characteristics on various agar media, diaminopimelic acid type, and morphological characteristics by scanning electron microscopy were examined. As a result, actinomycete isolate NH 50 was classified as a rare actinomycete that had LL-DAP type and did not produce spores. After incubation of isolate NH 50 in yeast extract-malt extract-dextrose broth, antifungal compound NH-B1 that inhibited mycelial growth of some plant pathogenic fungi was purified from the methanol eluates of XAD-16 resins by a series of purification procedures, i.e., silica gel flash chromatography, C18 flash chromatography, Sephadex LH-20 column chromatography, silica gel medium pressure liquid chromatography (MPLC), C18 MPLC, and high pressure liquid chromatography (HPLC). UV spectrum and 1HNMR spectrum of antifungal compound NH-B1 dissolved in methanol were examined. The antibiotic NH-B1 showed the major peaks at 230 and 271.2nm. Based on the data of 1H-NMR spectrum, NH-B1 was confirmed to be an extremely complex polymer of sugars called polysaccharides. The antibiotic NH-B1 showed strong antifungal activity against Alternaria solani and Cercospora kikuchi, but weak activity against M. grisea.

• Natural Product Sciences
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• v.21 no.4
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• pp.261-267
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• 2015

Compared to their terrestrial and marine counterparts, little is known about the capacity of freshwater-derived actinomycete bacteria to produce novel secondary metabolites. In the current study, we highlight the disparities that exist between cultivation-independent and -dependent analyses of actinomycete communities from four locations in Lake Michigan sediment. Furthermore, through phylogenetic analysis of strains isolated from these locations, we identified a Streptomyces sp., strain B025, as being distinct from other Streptomyces spp. isolated from sediment. Upon fermentation this strain produced a rare class of eight-membered lactone secondary metabolites, which have been for their antitumor properties. We used spectroscopic and chemical derivitization techniques to characterize octalactin B (1) in addition to its corresponding novel, unnatural degradation product (2).

• Natural Product Sciences
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• v.21 no.4
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• pp.273-277
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• 2015

Comprehensive chemical analysis of extracts and fractions of marine actinomycete strains led to the discovery of a new minor secondary metabolite, salternamide E (1), from a saltern-derived halophilic Streptomyces strain. The planar structure of salternamide E (1) was elucidated by a combinational analysis of spectroscopic data including NMR, MS, UV, and IR. The absolute configuration of salternamide E (1) was determined by circular dichroism spectroscopic analysis. Salternamide E displayed weak cytotoxicity against various human carcinoma cell lines.

• Archives of Pharmacal Research
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• v.28 no.12
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• pp.1341-1344
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• 2005

Bioassay-guided fractionation of $CHCl_{3}$ extract from the fermentation broth of a sponge Mycale plumose-derived actinomycete Saccharopolyspora sp. nov., led to the isolation of two known prodigiosin analogs - metacycloprodigiosin (1) and undecylprodigiosin (2). These compounds exhibited significant cytotoxic activities against five cancer cell lines: P388, HL60, A-549, BEL­7402, and SPCA4. This is the first report on the significant cytotoxicity of metacycloprodigiosin (1) against human cancer cell lines.

• Korean Journal of Pharmacognosy
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• v.54 no.1
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• pp.16-20
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• 2023

KSM-2690 B (1), a peptide-polyketide hybrid compound, was discovered from an actinomycete strain (CJD 1) isolated from Dong-Baek hill on Jeju Island, Republic of Korea. The chemical structure of 1 was identified by using NMR, MS, and UV spectroscopic analyses. Careful analysis of 1D and 2D NMR data revealed that KSM-2690 (1) has an oxazole ring, a β-lactone-γ-lactam spirocycle ring, and both triene and diene structures. KSM-2690 B (1) showed inhibitory activities against E. coli at 200 ㎍/mL.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1997.06b
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• pp.59-71
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• 1997

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.282.2-282
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• 1995

No Abstract, See Full Text

• Microbiology and Biotechnology Letters
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• v.14 no.5
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• pp.377-383
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• 1986

About 300 cellulolytic actinomycetes isolated from soils were tested for their cellulase activities estimated by means of filter paper swelling and carboxymethyl cellulose saccharifying activity. Then, 16 isolates which had shown relatively high levels of CMCase activity were selected and examined for their abilities of $\beta$-glucosidase production. Among them strain No. 109 was found to have highest level of intracellular $\beta$-glucosidase, and selected for the further studies. In this paper, the cultural, morphological and physiological properties, and cell wall composition of strain No. 109 were described in relation to the taxonomic status of this actinomycete. Based on the results obtained in these experiments strain No. 109 was identified to be a similar species to Streptomyces tanashiensis.