• The Plant Pathology Journal
• /
• 제23권3호
• /
• pp.203-209
• /
• 2007

Infection structures were observed using a fluorescence microscope at the penetration sites on the leaves of potato plants pre-inoculated with the bacterial strains Pseudomonas putida TRL2-3, Micrococcus luteus TRK2-2, and Flexibacteraceae bacterium MRL412, which mediated an induced systemic resistance on potato plants against late blight disease caused by Phytophthora infestans. In order to compare the infection structures on the leaves expressing systemic acquired resistance, the leaves of potato plants pre-treated with DL-3-amino butyric acid (BABA) were also observed after challenge inoculation with the same pathogen. The infection structures were investigated. The total number of germination and appressorium formation of P. infestans were counted. Furthermore, the frequencies of fluorescent epidermal cells at the penetration sites, which indicate a defense response of plant cell, were estimated. There were no differences on the germination rates of the fungal cysts among the untreated control, BABA pre-treated, and bacterial strains pre-inoculated plants. However, appressorium formation was slightly decreased on the leaves of BABA pre-treated plants compared to those of untreated as well as bacterial strains pre-inoculated plants. Furthermore, the frequencies of fluorescent cells of BABA pre-treated and bacterial strains pre-inoculated were higher than that of untreated plants, indicating an active defense reaction of the host cells against the fungal attack. On the other hand, the pre-treatment with BABA caused a stronger fluorescent of epidermal cells at the penetration sites compared to the pre-inoculation with the bacterial strains. Interestingly, the frequency of fluorescent cells by BABA, however, was lower than that by the bacterial strains. Based on the results it is suggested that the infection structures showing resistance reaction on the leaves of potato plants were different between by pre-inoculation with bacterial strains and by pre-treatment with BABA against the late blight pathogen.

• 한국표면공학회지
• /
• 제31권4호
• /
• pp.181-190
• /
• 1998

Al ion plating was carried to improve corrosion resistance of Nd-based permanent magnet made by prwder molding method. The effects of applied votage, pressyre and temperature were investigated to find the reation between coating parameters and their properties. Density of coating layer increased with voltage and thus corrosion resistance improved. However when voltage was applied more than 1000V, corrosion resistance whet down because of resputtering effect. Good corrosion resistance was acquired when gas pressure was $5.0\times10^-2$>torr, which is satisfied momentum energy of Ar, Al ions as well as quantity of plasma. The layer coated in low temperature range have better surface density and corrosion resistance than in high temperature. This result is seemed due to the characteristics of substrate itself. All coating layers were showed stong adhesion with substrate.

• Clinical and Experimental Pediatrics
• /
• 제51권10호
• /
• pp.1031-1037
• /
• 2008

Macrolide antimicrobial agents including erythromycin, roxithromycin, clarithromycin, and azithromycin are commonly used in the treatment of respiratory tract infections in children. Newer macrolides that have structural modifications of older drug erythromycin show improved change in the spectrum of activity, dosing, and administration. However, recent studies reported that increasing use of macrolide antibiotics is the main force driving the development of macrolide resistance in streptococci. In particular, azithromycin use is more likely to select for macrolide resistance with Streptococcus pneumoniae than is clarithromycin use, a possible reflection of its much longer half life. Recently, erythromycin resistance rates of S. pneumoniae and Streptococcus pyogenes are rapidly increasing in Korea. Two main mechanisms of acquired macrolide resistance have been described, altered binding site on the bacterial ribosome encoded by the ermB gene and active macrolide efflux pump encoded by the mef gene. Relationship between the susceptibility of S. pneumoniae and the response to macrolides has been shown in studies of acute otitis media, but less clear in cases of pneumonia. This article reviews the spectrum of activity, pharmacokinetic properties, mechanisms of action and resistance, and clinical implication of resistance on the treatment of respiratory tract infections in children.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제6권4호
• /
• pp.252-263
• /
• 2001

Nematode infections compromise human health and reduce agricultural productivtiy. Experiments that exploit the powerful molecular genetics of the free-living nematode Caenorhabdl - elegans have contributed to our understanding of how the major classes of anthelmintic nema-tocides kill worms and how worms might evolve resistance to these drugs In C. elegans, as in parasites, benzimidixoles interfere with microtubule polyumerization the imidazothiazoles/tetra-hydropyrimidines activate nicotinic acetylcholine receptors, and the macrocyclic la ctones activate qlutamate-gate chloride chanels. Mutant alleles of genes that encode drug targes often confer resistance in C. elegans. Preliminary evidence suggests that alleles of homologous genes in parasites will, in many cases, also play a role in resistance. Thus information acquired from C. elegans can be usefully applied to understand the mechanisms of drug sensitivity and the genetics of resis-tance in parasites.

• Computers and Concrete
• /
• 제18권1호
• /
• pp.113-137
• /
• 2016

Extensive experimental studies on remarkable mechanical properties Polypropylene Fibre Reinforced Self-compacting Concrete (PFRSCC) have been executed, including different fibre volume fractions of Polypropylene fibers (0.25%, 0.5%, 0.75%, and 1%) and different water to cement ratios (0.21, 0.34, 0.38, and 0.41). The experimental program was carried out by using two hundred and sixteen specimens to obtain the impact resistance and mechanical properties of PFRSCC materials, considering compressive strength, splitting tensile strength, and flexural strength. Statistical and analytical studies have been mainly focused on experimental data to correlate of mechanical properties of PFRSCC materials. Statistical results revealed that compressive, splitting tensile, and flexural strengths as well as impact resistance follow the normal distribution. Moreover, to correlate mechanical properties based on acquired test results, linear and nonlinear equations were developed among mechanical properties and impact resistance of PFRSCC materials.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6849-6853
• /
• 2014

Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.

• Journal of Pharmaceutical Investigation
• /
• 제37권5호
• /
• pp.287-290
• /
• 2007

Intrinsic or acquired resistance to chemotherapeutic drugs is one of the major obstacles to effective cancer treatment. Hypoxia is widespread in solid tumors as a consequence of decreased blood flow in the tumor-derived neovasculature. The recent finding of a link between hypoxia and chemoresistance prompted us to investigate whether hypoxia induces doxorubicin resistance in human MCF-7 breast cancer cells. Low oxygen concentration decreased the doxorubicin sensitivity in MCF-7 cells. The expression of p-glycoprotein, a major MDR-related transporter, and those of apoptosis-related proteins (anti-apoptotic Bcl-2, Bcl-XL and pro-apoptotic Bax) were not altered by hypoxia in MCF-7 cells. Intracellular uptake of doxorubicin was significantly decreased under hypoxic conditions. Decreased cellular uptake of doxorubicin under hypoxia may contribute to causing doxorubicin resistance in these cells. The use of agents that can modulate the doxorubicin uptake for adjuvant therapy may contribute to improving the therapeutic efficacy of doxorubicin in breast cancer patients.

• 전자공학회논문지T
• /
• 제36T권4호
• /
• pp.88-93
• /
• 1999

본 논문에서는 한방의료에서 인체 전신의 상태를 파악하는데 주요한 요소인 맥파를 과학적으로 관찰하고자 스트레인 게이지를 이용하여 맥파를 검출하는 새로운 시스템을 구성하였다. 스트레인 게이지는 센서의 변형에 대해 선형적으로 고유한 저항값이 변화되므로 손목에서 맥의 변화는 스트레인 게이지를 이용하여 맥파의 파형으로 검출할 수 있다. 맥파 검출 시스템의 구성에서 스트레인 게이지로부터 발생된 저항변화값을 검출하기 위해 정밀 브리지회로와 고감도 증폭회로를 설계, 제작하였으며 이 시스템으로 미세한 맥의 변화를 상응하는 전압파형으로 출력시킨다. 구성된 시스템을 이용하여 6인의 맥을 직접 검출하고 시간영역과 주파수 영역에서 맥의 특성을 관찰하므로써 본 연구에서 구성된 맥파 검출 시스템의 과학적인 효용성을 입증하였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권10호
• /
• pp.4147-4156
• /
• 2015

Lung cancer is a serious health problem and leading cause of death worldwide due to its high incidence and mortality. More than 80% of lung cancers feature a non-small cell histology. Over few decades, systemic chemotherapy and surgery are the only treatment options in this type of tumor but due to their limited efficacy and overall poor survival of patients, there is an urge to develop newer therapeutic strategies which circumvent the problems. Enhanced knowledge of translational science and molecular biology have revealed that lung tumors carry diverse driver gene mutations and adopt different intracellular pathways leading to carcinogenesis. Hence, the development of targeted agents against molecular subgroups harboring critical mutations is an attractive approach for therapeutic treatment. Targeted therapies are clearly more preferred nowadays over systemic therapies because they target tumor specific molecules resulting with enhanced activity and reduced toxicity to normal tissues. Thus, this review encompasses comprehensive updates on targeted therapies for the driver mutations in non-small cell lung cancer (NSCLC) and the potential challenges of acquired drug resistance faced i n the field of targeted therapy along with the imminent newer treatment modalities against lung cancer.

• 한국균학회지
• /
• 제35권1호
• /
• pp.43-46
• /
• 2007

식물에 대해 병원성균이 아닌 자연계에서 존재하는 진균류로 부터 세포벽 성분을 분리하여 식물의 유도 저항성을 유도하는 엘리시터로써의 가능성을 시험하였다. 그 결과 선발된 진균 세포벽 성분이 감자에서 종래 알려진 어떤 엘리시터 보다도 아주 낮은 농도처리에서도 활성산소와 파이토알렉신 생성을 다량 유도하였다. 뿐 만 아니라 감자괴경을 이용한 병저항성 생물검정에서도 우수한 유도저항성이 발현되었음을 확인하였다. 그러므로 자연에서 분리된 일반적인 균주의 세포벽물질 엘리시터도 식물체의 유도저항성을 효과적으로 유도할 수 있는 새로운 식물병방제제로서의 개발 가능성이 있음을 시사하였다.