• Journal of Microbiology and Biotechnology
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• 제7권3호
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• pp.189-193
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• 1997

Cryptosporidium parvum has been recognized as a significant cause of life-threatening diarrhea in Acquired ImmunoDeficiency Syndrome (AIDS) patients. Clinical diagnosis of cryptosporidial infections has been primarily based on the detection of infective stage, oocysts, in stools. Anti-Cryptosporidium oocyst monoclonal antibody (mAb), IgG2a, recognizing an antigen of 97 kDa was generated to be used for diagnosis of Cryptosporidium infection in AIDS patients using an immunofluorecence. It appeared to react with the surface antigens. Transmission electron micrographs of the infective stage of Cryptosporidium recognized by this mAb demonstrated sporolulated oocysts, which measure $4~6{\mu}m$, and sporozoites excysting from oocysts.

• Journal of Ginseng Research
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• 제35권4호
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• pp.413-420
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• 2011

We investigated whether Korean red ginseng (KRG) and highly active antiretroviral therapy (HAART) affect the frequency of gross deletion in 5'LTR/gag in 20 hemophiliacs. This study is a prospective study in 20 hemophiliacs who were infected with Korean subclade B of HIV-1 from two cash-paid plasma donors in 1990. Over a 13-year period, we obtained 436 amplicons of 5'LTR/gag genes by nested polymerase chain reaction using 147 peripheral blood mononuclear cells. Of the 436 amplicons, 92 (21.1%) showed gross deletion in 5'LTR/gag. Despite of a 2.3-fold higher monthly dose of KRG intake, the frequency of gross deletion in 5'LTR/gag (16.4%) was significantly decreased during HAART compared with 28.1% prior to HAART (p<0.01). Gross deletion in 5'LTR/gag was 10% more detected on KRG-therapy than prior to KRG-therapy (p<0.05). In addition, we also obtained 28 amplicons containing premature stop codon or isoleucine at initiation codon of 254 amplicons sequenced on KRG intake (7.5%) or HAART (13.6%) compared with 0% before KRG intake. These findings indicate that high frequency of gross deletion in 5'LTR/gag and genetic defects prior to HAART are significantly associated with KRG intake and the detection of gross deletion in 5'LTR/gag is decreased by HAART.

• Journal of Pharmaceutical Investigation
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• 제33권2호
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• pp.135-140
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• 2003

Fluconazole is an orally active bis-triazole antifungal agent, which is used in the treatment of superficial and systemic candidiasis and in the treatment of cryptococcal infections in patients with the acquired immuno deficiency syndrome (AIDS). The purpose of the present study was to evaluate the bioequivalence of two fluconazole capsules, Diflucan (Pfizer Pharmaceuticals Korea Inc.) and Flucona (Korean Drug Pharmaceuticals Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The fluconazole release from the two fluconazole capsules in vitro was tested using KP VII Apparatus II method at 0.1 M hydrochloride dissolution media. Twenty normal male volunteers, $23.60{\pm}1.88$ years in age and $63.57{\pm}6.17\;kg$ in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After three capsules containing 50 mg as fluconazole was orally administered, blood was taken at predetermined time intervals and the concentrations of fluconazole in serum were determined using HPLC method with UV detector. The dissolution profiles of two fluconazole capsules were very similar at 0.1 M hydrochloride dissolution media. Besides, the pharmacokinetic parameters such as $AUC_t,\;C_{max}\;and\;T_{max}$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$ and $C_{max}$ and untransformed $T_{max}$. The results showed that the differences in $AUC_t,\;C_{max}\;and\;T_{max}$ between two capsules based on the Diflucan were 4.96%, 5.65% and -13.76%, respectively. There were no sequence effects between two capsules in these parameter. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) $(e.g.,\;log(1.01){\sim}log(1.08)\;and\;log(1.00){\sim}log(1.12)\;for\;AUC_t\;and\;C_{max},\;respectively)$, indicating that Flucona capsule is bioequivalent to Diflucan capsule.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.187-196
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• 2020

본 연구는 동영상 강의를 통한 간호대학생의 HIV/AIDS교육의 효과를 확인하기 위해 실시된 단일군 사전사후 연구이다. 본 연구의 대상자는 J시 V대학교 간호학과 4학년 학생 93명으로, 자료수집기간은 2020년 5월 26일부터 6월 16일까지였으며, 수집된 자료는 SPSS Win 23.0 프로그램을 이용하여 기술통계, paired t-test, independent t-test, ANOVA, Pearson's correlation coefficient로 분석하였다. 본 연구결과 동영상 강의 전보다 후에 HIV/AIDS 지식과 태도가 향상되었음을 확인하였다. 그러나 태도의 세부항목 중 사회적 낙인인식의 차이는 미흡했다. 또한 지식과 태도에 대한 상관관계에서 지식이 향상되었다고 해서 태도가 긍정적으로 변화되는 것은 아니었으므로, 정확한 지식을 기반으로 하되 HIV/AIDS에 대한 인식개선을 좀 더 강조하는 교육이 필요하다. 그러므로 사회적 낙인인식을 긍정적으로 변화시킬 수 있는 교육내용 및 방법에 대한 다양한 시도가 필요하다. 본 연구결과를 통하여 동영상 강의가 간호대학생의 HIV/AIDS 지식과 태도를 변화시키는데 유용한 교수학습 방법임이 입증되었으며, 본 연구는 추후 다양한 컨텐츠를 동영상 강의 방법으로 활용하여 교육할 수 있는 근거를 마련하였다는데 의의가 있다.