• 한국미생물·생명공학회지
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• 제22권4호
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• pp.443-445
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• 1994

Eubacterium is one of the predominant bacteria in the human large intestine. currently ES (Eubacterium Selective) medium developed by T. Mitsuoka is commonly used as a selective medium. neomycin sulfate which is one of the selective agents of ES medium inhibited about 50% of the growth of Eubacterium isolated, whereas malidixic acid inhibited only 5% while inhibiting other intestinal bacteria. NES medium which replaced neomycin with nalidixic acid in the ES medium was designed and shown to be better for the isolation and enumeration of Eubacterium sp. than ES medium.

• Reproductive and Developmental Biology
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• 제28권1호
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• pp.29-36
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• 2004

본 연구는 체외에서 한우 난포란의 핵성숙과 그 후의 초기 배발달에 있어서 체외성숙 배지에 아미노산의 첨가가 난포란의 제1극체(PB) 출현율, 배발달율 그리고 배반포의 세포수에 미치는 영향을 검토하였다. 첨가하는 아미노산의 종류와 농도는 각각 MEM 배지의 non-essential amino acids (NEAA)와 BME 배지의 essential amino acids(EAA)는 1, 2, 4배 및 유청중의 lactalbumine hydrolysate(LAH)는 1, 5, 10 mg/$m\ell$이었다. 그 결과 NEAA와 EAA의 경우 PB 출현율은 1배 첨가군이 미첨가군보다 유의하게 높았지만(p＜0.05), 첨가량이 증가할수록 오히려 감소하였다. 그러나 배반포로의 배발달율은 모든 군에서 비슷한 경향이었다. 그리고 배반포의 총 세포수와 총 세포수중 TE 세포수는 2배 처리군이 가장 높았으며, ICM 세포수는 아미노산 첨가량이 증가할수록 많아졌다. 한편 LAH의 경우 PB 출현율은 5mg 첨가군이 가장높았으며, 배반포로의 발달율은 미첨가군과 1 mg 첨가군이 5 mg 첨가군과 10 mg 첨가군보다 각각 유의하게 높았다(p＜0.05). 그리고 배반포의 세포수는 NEAA와 EAA를 이용하였을 경우와 비슷한 경향이었다. 이상의 결과로부터 체외성숙용 배지에 아미노산의 첨가는 생산된 배반포의 품질을 향상시킬 수 있기 때문에 배반포의 체외생산에 이용할 수 있는 새로운 아미노산의 종류 및 농도를 탐색할 필요가 있다고 사료된다.

• 미생물학회지
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• 제22권3호
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• pp.135-142
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• 1984

The effect of biotin on the growth and sporulation of Bacillus subtilis SNU816 was investigated. When B. subtilis SNU816 was cultured on glucose as a sole carbon source, the growth was retarded markedly and usually ceased at early log phawe. But by addition of biotin to this medium, normal, rapid growth was restored. The growth rate was increased proportionally according to the concentration of exogenous biotin until it reached to 0.05㎍/ml, at which about three fold rapid growth was achieved. Also biotin was required for optimum sporulation for it facilitated the complete utilization of both glucose(Glc) and glutamic acid(Glu). Without biotin in Glc+Glu medium, about 40% of glutamic acid was remained unutilized. The dipicolinic acid content of cells cultured in Glc+Glu medium without biotin was markedly small and sporulation was suppressed before free spore release. Since biotin could be partiallyreplaced by one of TCA cycle intermediates such as oxalacetic acid, citric acid, or glutamic acid in enhancing growth in Glc medium, it was postulated that this strain might have a defect in converting pyruvate to oxalacetate which process is known to be mediated by pyruvate carboxylase that requires biotin as a cofactor.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.47-55
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• 2013

Metabolic alterations of Cordyceps bassiana mycelium were investigated under the following culture medium and light conditions: dextrose agar supplemented with 0.5% yeast extract (SDAY) medium with light (SL), SDAY medium without light (SD), nut medium without light (ND), and iron-supplemented SDAY medium without light (FD). The levels of asparagine, aspartic acid, glutamic acid, glutamine, histidine, lysine, ornithine, and proline were significantly higher under SD and SL conditions. The levels of most of the alcohols, saturated fatty acids, unsaturated fatty acids, fatty acid esters, sterols, and terpenes were higher under the ND condition than in the other conditions, but beauvericin was not detectable under the ND condition. The FD condition was favorable for the enhanced production of aminomalonic acid, malic acid, mannonic acid, and erythritol. Thus, the metabolic characteristics of C. bassiana can be manipulated by varying the cultivation conditions, rendering this fungus potentially favorable as a nutraceutical and medicinal resource.

• 한국미생물·생명공학회지
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• 제6권4호
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• pp.161-165
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• 1978

자연계에서 유기산을 생성하는 균주 11 군주를 분리하여 그 중에 구연산 생성균 1종을 선정하여, 동정한 결과 Aspergillus niger로 나타났다. 분리된 균주로 500ml 삼각 flask에 Sakaguchi 배지 100ml를 넣고 3$0^{\circ}C$에서 발효한 결과 구연산이 17g/ι가 생성되었다. 또한 Sakagnchi 배지를 개량한 결과 즉 배지적응, peptone첨가, 통기 효과, methanol첨가 및 포도당 첨가 결과 구연산이 35g/ι가 생성되었다

• 한국식물병리학회지
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• 제14권6호
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• pp.598-602
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• 1998

Twenty two rhizobacteria were isolated from the roots and rhizosphere of radish, carnation, potato and tomato. There isolates produced a fluorescent pigment in King's B medium and identified as Pseudomonas spp. These isolates colonized roots and rhizosphere of the host plants. In the study of cultural characteristics of the bacteria, the pH of the culture broth was changed from neutral (7.0) to alkali (8.8∼9.41) and the numbers of cells were increased from 106 to 108 after 40 hr of incubation in basal standard succinate medium. The salicylic acid production identified by pink color reaction were observed in 7 bacteria. Out of these 7 salicylic acid producing bacteria, only 2 strains of bacteria such as Pseudomonas fluorescens RS006, and Pseudomonas sp. EN401 were confirmed as salicylic acid producers by optical density measurement. Therefore, for screening of salicylic acid producing bacteria from the roots and rhizosphere, color reaction of the culture medium should be done in the first step, and then optical density measurement of culture extract should be made for the confirmation of salicylic acid production.

• Applied Biological Chemistry
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• 제38권6호
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• pp.485-489
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• 1995

T-flask와 air-lift 생물반응기를 이용하여 Autographa californica nuclear polyhedrosis virus (AcNPV)의 Tn5B1-4 세포에의 감염시 fatty acid 및 lipid, mannose, folic acid, $CaCl_2$등의 배지 첨가물이 재조합 ${\beta}-galactosidase\;({\beta}-gal)$ 생산에 미치는 영향을 조사하였다. Cholesterol, tocopherol, tricaprylin 또는 mannose를 첨가하거나 folic acid를 보강첨가 했을 때 AcNPV의 재조합 ${\beta}-gal$ 생산은 향상되었으나 $CaCl_2$의 보강 첨가는 ${\beta}-gal$ 생산을 높이는데 효과적이지 못했다. Air-lift 생물반응기에서 0.34 mM cholesterol, 2.2 mM mannose 및 0.045 M folic acid를 보강 첨가한 배지를 이용하였을 때 재조합 ${\beta}-gal$ 생산은 basal medium에 비해 약 2배 정도의 증진 효과가 있었다.

• 대한화학회지
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• 제58권1호
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• pp.85-91
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• 2014

The corrosion behavior of aluminum and mild steel in hydrochloric acid medium was studied using a nicotinic acid hydrazide as inhibitor by potentiodynamic polarization, electrochemical impedance spectroscopy technique and gravimetric methods. The effects of inhibitor concentration and temperature were investigated. The experimental results suggested, nicotinic acid hydrazide is a good corrosion inhibitor for both aluminum and mild steel in hydrochloric acid medium and the inhibition efficiency increased with increase in the inhibitor concentration. The polarization studies revealed that nicotinic acid hydrazide exhibits mixed type of inhibition. The inhibition was assumed to occur via adsorption of the inhibitor molecules on the aluminum and mild steel surface and inhibits corrosion by blocking the reaction sites on the surface of aluminum.

• 한국식품영양과학회지
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• 제26권5호
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• pp.872-877
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• 1997

Soybean curd whey(SCW) containing plenty of nutrients is the discarded by-product in soybean curd processing. To test the potential utilization of SCW as a medium for the cultivation of Lactobacilus acidophilus, the chemical composition of SCW, as well as the growth, acid production, acid-tolerance, and bile-tolerance of L. acidophilus in SCW-based media were investigated. Sucrose and stachyose, the main free sugars of SCW, were 0.42% and 0.41%, respectively. SCW contained 36.1mg/L of total free amino acids. L. acidophilus KFRI 150 showed lower cell growth and acid production in SCW than those in MRS broth. In optimized SCW-based medium supplemented with 1.0% glucose, 0.5% yeast extract, and 0.2% $K_{2}HPO_{4}$, the growth and acid production of L. acidophilus KFRI 150 increased by twice against those in SCW. In optimized SCW-based medium, the viable counts of four L. acidophilus strains were mostly at the level of $10^{9}$/ml, which is similar to those in MRS broth. Each acid-tolerance and biletolerance of four L. acidophilus strains cultured in optimized SCW-based medium and MRS broth showed no dist-inguishable difference.

• 미생물과산업
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• 제26권1호
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• pp.18-30
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• 2000

질소 고정능이 있는 Nostoc pruniforme를 재료로, 3종의 유기물, glucose, oxalic, 및 ethanol을 N-free medium과 NO$^{[-993]}$ riched medium에 처리해 주었을 때 다음과 같은 효과를 얻었다. 1)N-free medium과 NO$^{[-993]}$ riched medium에서 위의 유기물 모두가 질소 고정과정의 energy원으로서 또는 substrate로서의 유효한 결과를 얻었다. 3)oxalic acid를 처리한 실험에서는NO$^{[-993]}$ richedmedium의 경우 고농도 처리구에서 질소고정량이 많았고 N-free medium은 그 반대의 결과였다. 4)비교구인 control의 질소 고정량은 일예를 제회하고는 전반적으로 고농도 처리구와 저농도 처리구의 중간에 위치했다.