• 대한본초학회지
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• 제26권2호
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• pp.51-57
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• 2011

Objectives : Achyranthes japonica (AJ) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages. Methods : To measure out the cytotoxicity of AJ, we performed the MTT assay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen activated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB). Results : AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, western blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellular signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB. Conclusions : These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflammatory diseases.

• 대한한의학회지
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• 제25권4호
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• pp.8-14
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• 2004

Objective : Achyranthes japonica Nakai (AJ) has been classified as a herb that activates blood flow and clears the stagnated blood. In this study, we evaluated its anti-nociceptive and anti-inflammatory activity in animals to clarify the effect of AJ on pain or inflammation. Methods : ICR mice and Sprague-Dawley rats were pretreated with an ethanolic extract of AJ with two dosages of 200 mg/kg (p.o.) and 400 mg/kg (p.o.). Nociceptive responses of acute pain were determined by hotplate and tail-flick tests. The effects of AJ on inflammation were evaluated by flexion/extention test and mechanical hyperalgesia test in models induced by both carrageenan and Complete Freund's Adjuvant (CFA). Results : AJ showed significant analgesic effects in both hotplate and tail-flick tests at the dose of 400 mg/kg. It also produced a significant inhibition of carrageenan-induced paw edema and CFA induced arthritis in rats at the dose of 400 mg/kg. Conclusion : We have demonstrated the analgesic and anti-inflammatory properties of an 80% ethanolic extract of AJ in animals. This suggests the application of AJ in relief of pain or inflammatory disease.

• 한방재활의학과학회지
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• 제21권2호
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• pp.143-158
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• 2011

Objectives : Peripheral nerve injuries are commonly encountered clinical problem and often result in severe functional deficits. The aim of this study is to evaluate the effects of aqueous extract of Achyranthes japonica(AJ) on functional recovery in sciatic nerve after crushed sciatic nerve injury. Methods : In the present study, the animals in the AJ-treated groups received the aqueous extract of AJ at the respective doses orally for 13 consecutive days. In order to assess the effects of the aqueous extract of AJ on function recovery in crushed sciatic nerve injury, sciatic functional index(SFI) was performed. c-Fos expression in the paraventricular nucleus(PVN) and ventrolateral periaqueductal gray(vIPAG), and neurofilament, and the expressions of brain-derived neurotrophic factor(BDNF), nerve growth factor(NGF) following crushed sciatic nerve injury in rats were investigated. For this, immunohistochemistry and western blot were performed. Results : In the present study, crushed sciatic nerve injury showed characteristic gait changes showing decrease of SFI value and treatment with the aqueous extract of AJ significantly enhanced the SFI value. Neurofilament expression in the sciatic nerve was decreased by crushed sciatic nerve injury and treatment with the AJ increased neurofilament expression. The expressions of BDNF and NGF in the sciatic nerve were increased following crushed sciatic nerve injury and treatment with the AJ significantly controlled the sciatic nerve injury-induced increment of BDNF and NGF expressions. c-Fos expressions in the PVN and vIPAG were increased following crushed sciatic nerve injury and treatment with the AJ significantly suppressed the sciatic nerve injury-induced increment of c-Fos expressions. Conclusions : These results suggest that AJ treatment after crushed sciatic nerve injury is effective in the functional recovery by enhancing axonal regeneration and suppressing of pain.

• 공업화학
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• 제30권4호
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• pp.479-486
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• 2019

본 연구는 RAW264.7 대식세포에 대한 우슬과 땅두릅 복합 열수추출물(1 : 2, 1 : 3, 1 : 5, 2 : 1, 3 : 1 및 5 : 1 비율)의 항염증 효과를 조사했다. 세포 독성은 CCK 분석을 사용하여 확인하였다. 우리는 ELISA 키트를 사용하여 $IL-1{\beta}$, IL-6 및 $TNF{\alpha}$를 측정함으로써 우슬 및 땅두릅 복합 열수추출물의 항염증 효과를 평가하였다. 우슬 및 땅두릅 복합 열수추출물은 LPS로 자극된 RAW264.7 대식세포에서 $IL-1{\beta}$ 및 $TNF{\alpha}$를 유의적으로 억제하였다. 복합 추출물의 다양한 비율을 비교하였을 때, 2 : 1 비율은 LPS로 유도된 RAW264.7 세포에서 훨씬 더 효능이 있었고 $TNF{\alpha}$의 생성 또한 억제하였다. 본 연구의 결과는 우슬 및 땅두릅 복합 열수추출물이 RAW264.7 대식세포에 대해 강력한 항염증 효과를 갖는다는 것을 보여주며, 이들 추출물은 염증 질환 예방을 위한 기능성 식품의 좋은 공급원이 될 수 있다고 사료된다.

• 공업화학
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• 제30권2호
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• pp.145-150
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• 2019

본 연구에서는 우슬(Achyranthes japonica, AJ)과 Lactobacillus plantarum으로 발효한 우슬(AJ-LP)을 5가지 용매(물, 에탄올, 헥산, 아세트산에틸, 부탄올)로 추출하여 RAW264.7 세포에서 항염증 활성에 미치는 영향을 확인하였다. lipopolysaccharide(LPS)로 유도된 RAW264.7 세포에서 nitric oxide (NO) 및 cytokine 생성을 측정하였으며 western blot으로 cyclooxygenase-2(COX-2)와 nitric oxide synthase (iNOS)의 발현 정도를 관찰하였다. 세포 독성은 CCK assay를 통해 확인하였으며 독성을 가지지 않는 농도인 $100{\mu}g/mL$로 실험을 진행하였다. NO 생성 저해 활성 결과 AJ-LP를 에탄올(E)로 추출한 시료가 LPS만 처리한 control군에 비해 약 74%로 저해율이 가장 높았으며 염증관련 cytokine인 Interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and Interleukin-$1{\beta}$ (IL-$1{\beta}$)의 결과에서도 약 57, 70, 74%의 저해율로 우수한 효능을 보였다. COX-2와 iNOS 발현결과 AJ군에서 대조군(20-hydroxyecdysone)의 저해율이 가장 높은 것으로 나타났다. AJ-LP군에서 COX-2 발현량은 헥산(H)으로 추출한 시료가 대조군에 비해 약 16%로 감소하였고 iNOS 발현량은 부탄올(B)으로 추출한 시료가 약 7%로 감소하였다. 이상의 결과들에서 우슬보다 L. plantarum으로 발효한 우슬이 천연물 소재로서의 활용 가능성이 높음을 시사하였다.

• 대한한방부인과학회지
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• 제18권1호
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• pp.64-80
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• 2005

The pharmacological effects of medicinal remedies traditionally used in Asian countries for improving the blood circulation were examined on isolated rat thoracic aorta strips in organ baths. Each experimental medicine was consecutively extracted under reflux with water. Of 17 plants, Curcuma longa (CL) having the strongest acute relaxant activity in endothelium-intact arteries, Mucunae caulis (MC), Cirsium pendulum (CP), Rumex longiflius (RL), Paeonia suffruticosa (PS), Curcuma zedoaria (CZ), Scirpus maritimus (SM), Siphonostegia chinensis (SC), Leonurs sibiricus (LS) and Typha orientalis (TO) were showing dose-dependent relaxant activity. Long-term relaxant effects were showed in Curcuma aromatia (CA), MC, CP, RL, PS, Potulacae grandiflorae (PG), CZ, Panax notoginseng (PN), Achyranthes japonica (AJ), CL, SC, Lycoppus lucidus (LL) and Corydalis turtschaninovii (CT). In endothelium-injury test using carbachol, CL, SC, MC, RL and PS which are having the acute vasorelaxing activity and CA and CT which are not showing vasorelaxaing activity were damaged to endothelium. As a result of this study, the possibility that a part of medicinal remedy may contribute to the beneficial effects in blood circulation was proposed, but inter-individual variation has been observed. Also, further studies on the vasorelaxant effects of these remedies are still required.