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http://dx.doi.org/10.14478/ace.2019.1049

Anti-inflammatory Effects of Achyranthes japonica Nakai and Aralia continentalis Kitagawa Complex Fermented Extracts on LPS-stimulated RAW264.7 Macrophage  

Woo, Young Min (Natural Science Institute, Silla University)
Jo, Eun Sol (Natural Science Institute, Silla University)
Kim, Ok Ju (Natural Science Institute, Silla University)
Lee, Young-Ho (Protein Structure Research Group, Division of Bioconvergence Analysis, Korea Basic Science Institute)
Ahn, Mee Young (Department of Pharmaceutical Engineering, Silla University)
Lee, Dong-Geun (Department of Pharmaceutical Engineering, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, Silla University)
Kim, Andre (Department of Pharmaceutical Engineering, Silla University)
Publication Information
Applied Chemistry for Engineering / v.30, no.4, 2019 , pp. 479-486 More about this Journal
Abstract
This study investigated the anti-inflammatory effects of mixed extracts of Achyranthes japonica Nakai (Aj) and Aralia continentalis Kitagawa (Ac) (ratios of 1 : 2, 1 : 3, 1 : 5, 2 : 1, 3 : 1 and 5 : 1) on RAW264.7 macrophages. Cell toxicity was determined using a cell counting kit (CCK) assay. We evaluated anti-inflammatory effects of the mixed extracts of Aj and Ac by measuring interleukin $(IL)-1{\beta}$, IL-6, and tumor necrosis factor $(TNF){\alpha}$ using an enzyme-linked immunosorbent assay (ELISA) kit assay. The mixed extracts of Aj and Ac inhibited lipopolysaccharide (LPS)-induced $IL-1{\beta}$ and $TNF{\alpha}$ in LPS-stimulated macrophages. Comparing different ratios of the mixed extracts, the 2 : 1 ratio of Aj and Ac has much more potency and inhibited the production of $TNF{\alpha}$ in LPS-induced RAW264.7 cells. The results of the present study showed that the mixed extracts of Aj and Ac have potential anti-inflammatory effects on RAW264.7 macrophages. Therefore, these extracts may be used as a good source of functional foods for the protection against inflammatory diseases.
Keywords
Achyranthes japonica Nakai (Aj); Aralia continentalis Kitagawa (Ac); Anti-inflammatory; Macrophage;
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