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http://dx.doi.org/10.6116/kjh.2011.26.2.051

The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages  

Kim, Min-Sun (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
Jeong, Jin-Soo (Dept. of Herbology, College of Oriental Medicine, Woosuk University)
Lee, Hye-Youn (Dept. of Herbology, College of Oriental Medicine, Woosuk University)
Ju, Young-Sung (Dept. of Herbology, College of Oriental Medicine, Woosuk University)
Bae, Gi-Sang (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
Seo, Sang-Wan (ChungBuk Techno Park Bio center)
Cho, Il-Joo (Dept. of Beauty science, Kwangju Woman's Unversity)
Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University)
Publication Information
The Korea Journal of Herbology / v.26, no.2, 2011 , pp. 51-57 More about this Journal
Abstract
Objectives : Achyranthes japonica (AJ) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages. Methods : To measure out the cytotoxicity of AJ, we performed the MTT assay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen activated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB). Results : AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, western blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellular signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB. Conclusions : These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflammatory diseases.
Keywords
Achyranthes japonica (AJ); cytokine; inflammation;
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1 Kim JH, Ki JY, Ann JY, Park HJ, Kim HJ, Kwak HB, Oh JM, Kim YK. Inhibitory effects of Achyranthis Bidentatae Radix on osteoclast differencitation and bine resorption. Kor J Herbology. 2010;25(1):65-74.
2 Hu SM, Zhou P, Fu Q, Yang JJ, Gao XM. Effect of a Chinese herbal prescription on collagen I in rat's femur under simulated weightlessness. Zhongguo Gu Shang. 2010;23(2):117-9.
3 Xie F, Li X, Sun K, Chu Y, Cao H, Chen N, Wang W, Liu M, Liu W, Mao D. An experimental study on drugs for improving blood circulation and removing blood stasis in treating mild chronic hepatic damage. J Tradit Chin Med. 2001;21(3):225-31.
4 Do WS, Kim KH, Kim KS. Effect of aqua-acupuncture(Cortex Ulmi Pumilae, Ramulus Cinnamomum, Radix achyranthis, Apitoxin.Calculus Bovis.Fel Ursi.Moschus compound) in mice with lipopolysaccharide induced arthritis. J Kor Acupuncture & Moxibustion Society. 2001;18(1):157-169.
5 Li XP. Experimental study on anti-senility of the 4 famous Chinese herbs produced in Huaiqing area. Zhong Xi Yi Jie He Za Zhi. 1991;11(8):486-7.
6 Jung SM, Choi SI, Park SM, Heo TR. Antimicrobial effect of Achyranthes japonica Nakai extracts against Clostrium difficile. Kor J Food Sci Technol. 2007;39(5):564-8.
7 Kim YO, Lee SW, Lee SE. Effects of Achyrantes japonica on carrageenan -induced arthritis rat model. Kor J Medicinal Crop. 2009;17(6):470-4.
8 Lee SD, Kim KS. The effect of Achyranthis Radix and Apitoxin Aqua- acupuncture on cellular immune responses to LPS-induced arthritis in mice. J Kor Acupuncture & Moxibustion Society. 1999;16(3):287-315.
9 Shin MK. Clinical Traditional Herbalogy. seoul:yonglin publisher, 2010:539-543.
10 Chinese Treditional medicine management agency <> commission. 1th edition vol 2. shanghai:shanghai technology publisher, 1999:830-837.
11 Riehemann K, Behnke B, Schulze-Osthoff K. Plant extracts from stinging nettle (Urtica dioica), an antirheumatic remedy, inhibit the proinflammatory transcription factor NF-kappaB. FEBS Lett. 1999;442(1):89-94.   DOI   ScienceOn
12 Smith SJ, Fenwick PS, Nicholson AG, Kirschenbaum F, Finney-Hayward TK, Higgins LS, Giembycz MA, Barnes PJ, Donnelly LE. Inhibitory effect of p38 mitogen-activated protein kinase inhibitors on cytokine release from human macrophages. Br J Pharmacol. 2006;149(4):393-404.   DOI   ScienceOn
13 Kang BS et al. Herbal medicine. 6th revised edition. seoul:yonglin publisher, 2000:427-428.
14 Underwood DC, Osborn RR, Kotzer CJ, Adams JL, Lee JC, Webb EF, Carpenter DC, Bochnowicz S, Thomas HC, Hay DW, Griswold DE. SB 239063, a potent p38 MAP kinase inhibitor, reduces inflammatory cytokine production, airways eosinophil infiltration, and persistence. J Pharmacol Exp Ther. 2000;293(1):281-8.
15 Baeuerle PA, Henkel T. Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994:141-9.
16 Zandi E, et al. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell. 1997;91(2):243-52.   DOI   ScienceOn
17 Sigala I, Zacharatos P, Toumpanakis D, Michailidou T, Noussia O, Theocharis S, Roussos C, Papapetropoulos A, Vassilakopoulos T. MAPKs and NF-{kappa}B differentially regulate cytokine expression in the diaphragm in response to resistive breathing： the role of oxidative stress. Am J Physiol Regul Integr Comp Physiol. 2011;300(5):R1152-62.   DOI
18 Chang JH, Park JY, Kim SK. Dependence on p38 MAPK signalling in the up-regulation of TLR2, TLR4 and TLR9 gene expression in Trichomonas vaginalis-treated HeLa cells. Immunology. 2006;118(2):164-70.   DOI   ScienceOn
19 Oh CH translation. simple immunology. seoul medical korea. 2006:161-200.
20 Lawrence T, et al. Possible new role for NF-kappaB in the resolution of inflammation. Nat Med. 2001:1291-7.
21 Ajizian SJ, English BK, Meals EA. Specific inhibitors of p38 and extracellular signal regulated kinase mitogen-activated protein kinase pathways block inducible nitric oxide synthase and tumor necrosis factor accumulation in murine macrophages stimulated with lipopolysaccharide and interferon-gamma. J Infect Dis. 1999;179:939-44.   DOI   ScienceOn
22 Horwood HJ, Page TH, McDaid JP, Palmer CD, Campbell J, Mahon T, Brennan FM, Webster D, Foxwell BM, Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6 production. L Immunol. 2006;176(6):3635-41.   DOI
23 Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem Sci. 2000;25:7-9.   DOI
24 Celec P. Nuclear factor kappa B-molecular biomedicine the nest generation. Biomed Pharmacother. 2004;58:365-71.   DOI   ScienceOn
25 Seo WG, Pae HO, Oh GS, Kim NY, Kwon TO, Shin MK, Chai KY, Chung HT. The aqueous extract of Rhodiola sachalinensis root enhances gene in RAW264.7 macrophage. J Ethnophamacol. 2001;76:119-23.   DOI   ScienceOn
26 Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxied biosythesis in RAW264.7 macrophages. Life Sci. 2001;69:625-35.   DOI   ScienceOn
27 Matsuda H, Morikawa T, Ando S, Toguchida I and Yoshikawa M. Structural requirements of Flavonoids for nitric oxied production inhibitory activity and mechanism of action. Bioorganic Med. Chem. 2003;11:1995-2000.   DOI   ScienceOn
28 Calixto JB, Campos MM, Otuki MF, Santos AR. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Med. 2004;70(2):93-103.   DOI   ScienceOn
29 Lee BG, Kim SH, Zee OP, Lee KR, Lee HY, Han JW, Lee HW. Suppression of inducible nitric oxide synthase expression in RAW264.7 macrophages by two-carboline alkaloids estracted from Melia azedarach. Eur J Pharmacol. 2000;406:301-9.   DOI   ScienceOn
30 Seo WG, Pae HO, Oh GS, Chai KY, Yun YG, Kwon TO, Chung HT. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxied synthase gene in RAW264.7 macrophages stimulated with interferon and lipopolysaccharide. Gen Pharmocol. 2000;35:21-8.   DOI   ScienceOn
31 Lu T, Mao C, Zhang L, Xu W. The research on analgestic and anti-inflammatory action of different processed products of Achyranthes bidentata. Ahong Yao Cai. 1997;20(10):507-9.
32 Nathan C, Xie QW, Nitric oxide synthases: roles, tolls and controls. Cell 1994;78:915-8.   DOI   ScienceOn
33 Kwqamata H, Ochiai H, Mantani N, terasawa K. Enhanced expression of inducible nitric oxied RAW264.7 cells, a murine macrophage cell line. Am J Chin Med. 2000;28:217-26.   DOI   ScienceOn
34 Aderem A Ulevitch RJ. Toll-like receptors in the induction of the innate immune response. Nature. 2000;406(6797):782-7.   DOI   ScienceOn
35 Hoshino K, Takeuchi O, Kawai T, Sanjo H, Ogawa T, Takeda Y, Takeda K, Akira S. Toll-like receptor4(TLR4)-deficient mice are hyporesponsive to lipopolysaccharide:evidence for TLR4 as the Lps gene product. J immunol. 1999;162(7):3749-52.
36 Chen XM, Tian GY. Structural elucidation and antitumor activity of a fructan from Cyathula officinalis Kuan Carbohydr Res.2003;338(11):1235-41.   DOI   ScienceOn
37 Gao XY, Wang DW, Li FM. Determination of ecdysterone in Achyranthes Bidentata Bl and its activity promoting proliferation of osteoblast-like cells. Yao Xue Xue Bao.2000;35(11):868-70.
38 Medzhitov R, Preston-Hurlburt P,Janeway CA Jr. A human homologue of the Drosophila Toll protein signal activation of adaptive immunity. Nature. 1997;888(6640):394-7.