• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.464-478
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• 2003

Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental facts play critical roles in cellular damage and aging. The extract of tinged autumnal leaves of maple tree(Acer palmatum) has proven to be a powerful antioxidant. The Acer palmatum extract is very effective on the stabilization of biological membranes( containing unsaturated fatty acid). We studied photoprotective effect of the extract against UVB-induced cytotoxicity in human keratinocytes. The extract improved cell viability comparing to control after UVB irradiation. In the determination test of pro inflammatory cytokines the extract decreased expression of interleukin 1 a and 6, which play an important role in inflammation and skin erythema caused by UV. We also studied property of varying cosmetic formulations on the percutaneous absorption of the extract. After 24 hour in vitro penetration study, the content of the extract was more highly detected in skin residue part. This result showed the extract had relatively high compatibility of skin in our emulsion system. On human skin, after appling the product containing the extract we obtained a good result of antiwrinkle effect by skin visiometer. In conclusion, the Acer palmatum extract is a photoprotective and very effective in stressed and aged skin care. And we can predict the extract mainly affects on the skin cell and tissue in our emulsion system.

• Korean Journal of Plant Resources
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• v.34 no.6
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• pp.566-574
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• 2021

This research was carried out to investigate the correlation between soil chemical properties and bioactive compounds of Acer tegmentosum Maxim. The methods of determining bioactive compounds were determined by high performance liquid chromatography, that contained (-)-gallocatechin (0.04±0.01 ~ 0.43±0.28%), salidroside (0.90±0.06 ~ 3.86±0.59%), tyrosol (0.03±0.00 ~ 0.43±0.00%), (-)-catechin (0.05±0.01 ~ 0.37±0.14%), 6'-O-galloylsalidroside (0.02± 0.01 ~ 0.31±0.06%), (-)-epicatechin-gallate (0.01±0.00 ~ 0.04±0.01%). The soil chemical properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P₂O₅), exchangeable cation and cation exchange capacity (CEC) were performed following the standard manual. The correlation analysis between soil chemical properties and bioactive compounds of A. tegmentosum, soil pH, available phosphate and exchangeable cation (Ca²⁺ and Mg²⁺) were negatively correlated with content of salidroside. On the other hand, soil exchangeable cation (Na⁺) showed positive correlation with content of salidroside. The results of this study was able to investigate the correlation between soil chemical properties and bioactive compounds of A. tegmentosum.

• Korean Journal of Pharmacognosy
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• v.53 no.4
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• pp.202-206
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• 2022

Salidroside is a major phenolic glycoside of Acer tegmentosum (Aceraceae) and known to be a hepatoprotective compound. Extraction conditions are one of the most important factors to save time and cost from an economic point of view. For this reason, this study was conducted to optimize the extraction condition for maximum yield of salidroside. For optimization, three extraction factors such as ethanol concentration (%), extraction temperature (℃), and solvent to material ratio (mg/mL) were tested and optimized for maximum yield of salidroside using response surface methodology (RSM). The optimal condition was obtained as an ethanol concentration of 53.4%, an extraction temperature at 67.11℃ and a solvent to material ratio (mg/mL), 195.55 mg/ml. The salidroside yield under optimal conditions was found to be 1.59 mg/g dried samples, which were well-matched with the predicted value of 1.56 mg/g dried samples. These results will provide useful information about optimized extraction conditions for the development of salidroside as hepatoprotective therapeutics.

• Journal of Korean Society of Forest Science
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• v.42 no.1
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• pp.67-73
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• 1979

In order to observe the anatomical phenomena of root formation in the hypocotyl cuttings of maples, the hypocotyls of Acer palmatum. Acer micro-sieboldianum, and Acer saccharinum were used as the materials. The rooted portions were sectioned by a microtom and doubly stained by safranin and fast green. The results obtained are summarized as follows; 1. A cross section structure of hypocotyl cuttings of Acer sp. is consist of epidermis, cortex, vascular bundle and pith from the outside. The vascular bundle makes circular shape forming polyarch in A. saccharinum and tetrarch in both A. palmatum and A. micro-sieboldianum. 2. An adventitious root of A. saccharinum originated in phloem parenchyma, while that of A. palmatum and A. micro-sieboldianum originated in interfascicular parenchyma related with phloem cells. 3. The hypocotyl cuttings of Acer sp. is commonly composed of parenchyma tissue having vigorous differentiation capability. Therefore, the originated root grow easily through the cortex and epidermis breaking their tissues.

• Journal of Korean Society of Forest Science
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• v.89 no.5
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• pp.552-563
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• 2000

The aim of this study was to characterize the forest vegetation and site of Odaesan National Park for ecological forest management. The vegetation could be divided into two forest community groups, seven forest communities, and several subcommunities. South exposed, dry slopes were covered with forests of the Quercus mongolica-Acer pseudosieboldianum community group. Quercus mongolica-Acer pseudosieboldianum community group could be subdivided into ${\bullet}$ Rhododendron mucronulatum var. ciliatum community on dry sites, especially on south or south-westly exposed ridges or convex slopes; ${\bullet}$ Lespedeza${\times}$tomentella community on south exposed slopes, at low altitudes in the eastern part of the study area; ${\bullet}$ Carex humilis-Maackia amurensis community on south exposed slopes up to the montane region; ${\bullet}$ Quercus mongolica-Acer pseudo-sieboldianum typical community on south exposed middle and low slopes, and on upper and middle north exposed slopes; ${\bullet}$ Pimpinella brachycarpa community, preferably on moderately moist and cool sites. On north exposed, more humid and moist sites, forests of the Dryopteris crassirhizoma-Cornus controversa community group were found. This group was subdivided into ${\bullet}$ Acer tschonoskii var. rubripes-Acer tegmentosum community on moderately moist to moist, loamy soils on shady slopes; ${\bullet}$ Deutzia glabrata-Ulmus laciniata community on north exposed slopes with rocky soil and boulders, having high humidity. There are several rare and endangered species in Rhododendron mucronulatum var. ciliatum community, Carex humilis-Maackia amurensis community, Vicia venosa var. cuspidata subcommunity and Saxifraga punctata subcommunity, and the communities were located in azonal areas in topography. To protect the species, the areas should be categorized as an absolute conservation area, and any forest working should be prohibited in this area.

• Journal of Korean Society of Forest Science
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• v.99 no.1
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• pp.16-23
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• 2010

Experiments were performed for investigate the immune activities on human B and T cell growth and secretion of their cytokines. Also, antibodies in serum were investigated in female ICR mouse by feeding the extracts of Acer mono at dose of 30 and 100 mg/mL of one day orally for 15 days. The cytotoxicity of the samples on normal human cell (HEL299) was below 24.15% in adding the all extracts. Both human immune B and T cells were increased up to about 40% by the high pressure extraction process. The secretion of cytokines (IL-6, TNF-${\alpha}$) on human B and T cells were increased up to 20-50% by adding the high pressure extraction process compare to the hot water extraction process. Also, total serum IgG levels increased by feeding Acer mono extacts. It can be conclude that optimum condition for efficient extraction of Acer mono as functional materials is solvent extraction process using water with high pressure at below $100^{\circ}CA$ than typical process.

• Korean Journal of Environment and Ecology
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• v.12 no.3
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• pp.236-241
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• 1998

This study was to compare soil characteristics among Quercus mongolica communities that characterize the boreal-temperate deciduous forest in Korea. The classification of Quercus mongolica community and soil sampling were carried in Mt. Odae and Mt. Jungwang in Kangwondo from April of 1991 to October of 1994. The study area was classified as 5 Quercus mongolica communities with Braun-Blanquet method as follows; Quercus mongolica - Abies nephrolepis, Quercus mongolica - Acer pseudosieboldianum, Quercus mongolica - Lindera obtusiloba, Quercus mongolica - Acer mandshuricum, and Quercus mongolica - Carpinus cordata communities. Quercus mongolica - Abies nephorolepis community had the most shallow depth of A horizon(5cm) among communities, and root penetration was mainly from 0 to 10cm soil depth, and they had a dry soil moisture condition. Depth of A horizon of Quercus mongolica - Lindera obtusiloba and Quercus mongolica - Acer mandshuricum communities was about 20cm, and root penetration was mainly from 0 to 20cm soil depth, and they had a slightly dry soil moisture condition. Quercus mongolica - Acer mandshuricum, and Quercus mongolica -Carpinus cordata communities had the deepest depth of A horizon(35cm) and root was well developed over 45cm, and they had a moderately-slight dry soil condition. The soil organic matter, total N, exchangeable Ca, Mg and K concentration and CEC was the greatest in Quercus mongolica-Acer mandshuricum community and Quercus mongolica-Carpinus cordata community among communities. Quercus mongolica - Abies nephyolepis community had the smallest soil organic matter, total N, and CEC among communities. There were large differences among Quercus mongolica communities by soil properties and the result may be due to different habitat positions in the landscape among communities.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.51-57
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• 1999

To search for antioxidative compounds from plant resources, methanol extracts of 45 plant species were investigated using DPPH method. The highest activity was shown in the methanol extract of Acer ginnala($RC_{50}\;:\;15{\mu}g$), followed by Stewartia koreana($RC_{50}\;:\;28{\mu}g$) and Carpinus laxiflora($RC_{50}\;:\;33{\mu}g$). Two antioxidative compounds were isolated from the methanolic extract of Acer ginnala Max and identified as acertannin(2, 6-di-O-galloyl-1, 5-anhydro-D-glucitol) and gallicin (methyl-3, 4, 5-trihydroxybenzoic acid) on the basis of mass spectroscopy, $^1H-\;and\;^{13}C-NMR$ data. The DPPH free radical scavenging activities of acertannin($RC_{50}\;:\;3.5{\mu}g$) and gallicin($RC_{50}\;:\;2.8{\mu}g$) were more effective than those of BHA($RC_{50}\;:\;14{\mu}g$) and ${\alpha}-tocopherol$ ($RC_{50}\;:\;12{\mu}g$).

• Journal of Korean Society of Forest Science
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• v.95 no.5
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• pp.614-620
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• 2006

To study the chlorophyll contents and photosynthetic characteristics of 4 tree species of deciduous hardwoods; Betula platyphylla var. japonica, Zelkova serrata, Acer mono and Prunus sargentii were treated in 3 stages of shading; the full sun treatment, the medium shade treatment with 30% of transmittance comparing to full sun, the intense shade treatment with 8% of transmittance and their changes in chlorophyll contents and photosynthetic characteristics were examined and analyzed. Most hardwoods showed differences in the total chlorophyll contents in the order of May < September < July, however, that in Prunus sargentii increased progressively along with the lapse of time. Concerning the degree of shading, total chlorophyll contents increased in proportion to the level of shading. Betula platyphylla var. japonica and Prunus sargentii showed more than 2-3 times difference between the full sun treatment and the intense shade treatment. The changes in photosynthetic characteristics, the range of the light saturation point of the trees was $1,000{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in May, before the shading was applied, and the intensity was shown in the order of Betula platyphylla var. japonica > Zelkova serrata > Acer mono > Prunus sargentii. The photosynthetic rate was $6.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}{\sim}27.1{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of Betula platyphylla var. japonica > Prunus sargentii > Acer mono > Zelkova serrata that there were differences between species. Concerning the changes in light saturation point in each growth period after shading treatment, the light saturation point in the full sun treatment was found in the range of $560{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and the level of intensity was shown in the order of May > July > September. The light saturation point decreased as the level of shading intensified and the level of changes in light compensation point in the full sun treatment for Betula platyphylla var. japonica and Prunus sargentii was shown in the range of $2.9{\sim}27.1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of May > July > September, however, for Zelkova serrata and Acer mono was shown in the range of $3.9{\sim}11.7{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of July > May > September that there were differences between species.

• Journal of Korean Society of Forest Science
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• v.100 no.1
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• pp.14-24
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• 2011

Anticancer activity of Acer mono aqueous extracts was enhanced by nano-encapsulation process of gelatin. The cytotoxicity on human normal lung cell (HEL299) of the extracts from WE (water extract at 100) showed 23.51%, lower than that from NE (nano-encapsulatioin of water extract of Acer mono) in adding the maximum concentration of 1.0 mg/mL. NE showed more potent scavenging effect as 73.15% than the WE. On SOD-like test, the NE showed highest activity as 32.33% at 1.0 mg/mL concentration. Human stomach adenocarcinoma, liver adenocarcinoma, breast adenocarcinoma and lung adenocarcinoma cell growth were inhibited up to about 59-73%, in adding 1.0 mg/mL of NE. NE was 15% higher than conventional water extraction. Among several cancer cell lines (stomach adenocarcinoma, liver adenocarcinoma), the growth of digestive related cancer cells were most effectively inhibited as about 71-73%. The size of nano particles was in the ranges of 100-200 nm, which can effectively the penetrate into the cells, it was observed by real time confocal microscope. It tells that the aqueous extracts of Acer mono bark could be definitely enhanced by nano-encapsulation process.