• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.12
• /
• pp.5147-5152
• /
• 2016

Background: Investigations of methods for detection of mutations have uncovered major weaknesses of direct sequencing and pyrosequencing, with their high costs and low sensitivity in screening for both known and unknown mutations. High resolution melting (HRM) analysis is an alternative tool for the rapid detection of mutations. Here we describe the accuracy of HRM in screening for KRAS and BRAF mutations in metastatic colorectal cancer (mCRCs) samples. Materials and Methods: A total of 1000 mCRC patients in Mehr Hospital, Tehran, Iran, from Feb 2008 to May 2012 were examined for KRAS mutations and 242 of them were selected for further assessment of BRAF mutations by HRM analysis. In order to calculate the sensitivity and specificity, HRM results were checked by pyrosequencing as the golden standard and Dxs Therascreen as a further method. Results: In the total of 1,000 participants, there were 664 (66.4%) with wild type and 336 (33.6%) with mutant codons 12 and/or 13 of the KRAS gene. Among 242 samples randomly checked for the BRAF gene, all were wild type by HRM. Pyrosequencing and Dxs Therascreen results were in line with those of the HRM. In this regard, the sensitivity and specificity of HRM were evaluated as 100%. Conclusion: The findings suggest that the HRM, in comparison with DNA sequencing, is a more appropriate method for precise scanning of KRAS and BRAF mutations. It is also possible to state that HRM may be an attractive technique for the detection of known or unknown somatic mutations in other genes.

• Journal of Korean Ophthalmic Optics Society
• /
• v.20 no.4
• /
• pp.501-509
• /
• 2015

Purpose: To evaluate the reliability of refractive power by comparing the marked refractive power in an automatic phoropter and actually measured spherical/cylindrical refractive power. Methods: Actual refractive power of minus spherical lens and cylindrical lens in an automatic phoropter was measured by a manual lensmeter and compared with the accuracy of marked refractive power. Furthermore, combined refractive power and spherical equivalent refractive power of two overlapped lenses were compared and evaluated with the refractive power of trial lens. Results: An error of 0.125 D and more against the marked degree was observed in 70.6% of spherical refractive power of spherical lens which is built in phoropter, and the higher error was shown with increasing refractive power. Single cylindrical refractive power of cylindrical lens is almost equivalent to the marked degree. Combined spherical refractive power was equivalent to spherical refractive power of single lens when spherical lens and cylindrical lens were overlapped in a phoropter. Thus, there was no change in spherical refractive power by lens overlapping. However, there was a great difference, which suggest the effect induced by overlapping between cylindrical refractive power and the marked degree when spherical lens and cylindrical lens were overlapped. Spherical equivalent refractive power measured by using a phoropter was lower than that estimated by trial glasses frame and marked degree. The difference was bigger with higher refractive power. Conclusions: When assessment of visual acuity is made by using an automatic phoropter for high myopes or myopic astigmatism, some difference against the marked degree may be produced and they may be overcorrected which suggests that improvement is required.

• The Journal of Industrial Distribution & Business
• /
• v.8 no.4
• /
• pp.47-52
• /
• 2017

Purpose - This current study will investigate the average financial ratio of top and failed five-star hotels in the Jeju area. A total of 14 financial ratio variables are utilized. This study aims to; first, assess financial ratio of the first-class hotels in Jeju to establishing variables, second, develop distress prediction model for the first-class hotels in Jeju district by using logit analysis and third, evaluate distress prediction capacity for the first-class hotels in Jeju district by using logit analysis. Research design, data, and methodology - The sample was collected from year 2015 and 14 financial ratios of 12 first-class hotels in Jeju district. The results from the samples were analyzed by t-test, and the independent variables were chosen. This was an empirical study where the distress prediction model was evaluated by logit analysis. This current research has focused on critically analyzing and differentiating between the top and failed hotels in the Jeju area by utilizing the 14 financial ratio variables. Results - The verification result of the accuracy estimated by logit analysis has shown to indicate that the distress prediction model's distress prediction capacity was 83.3%. In order to extract the factors that differentiated the top hotels in the Jeju area from the failed hotels among the 14 chosen, the analysis of t-black was utilized by independent variables. Logit analysis was also used in this study. As a result, it was observed that 5 variables were statistically significant and are included in the logit analysis for discernment of top and failed hotels in the Jeju area. Conclusions - The distress prediction press' prediction capability was compared in this research analysis. The distress prediction press prediction capability was shown to range from 75-85% by logit analysis from a previous study. In this current research, the study's prediction capacity was shown to be 83.33%. It was considered a high number and was found to belong to the range of the previous study's prediction capacity range. From a practical perspective, the capacity of the assessment of the distress prediction model in the top and failed hotels in the Jeju area was considered to be a prominent factor in applications of future hotel appraisal.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.10
• /
• pp.197-205
• /
• 2020

Compared to aerial photogrammetry, UAV photogrammetry has advantages in acquiring and utilizing high-resolution images more quickly. The production of 3D models using UAV photogrammetry has become an important issue at a time when the applications of 3D spatial information are proliferating. Therefore, this study assessed the feasibility of utilizing 3D models produced by UAV photogrammetry through quantitative and qualitative analyses. The qualitative analysis was performed in accordance with the LODs (Level of Details) specified in the 3D Land Spatial Information Construction Regulation. The results showed that the features on planes have a high LoD while features with elevation differences have a low LoD due to the occlusion area and parallax. Quantitative analysis was performed using the 3D coordinates obtained from the CPs (Checkpoints) and edges of nearby structures. The mean errors for residuals at CPs were 0.042 m to 0.059 m in the horizontal and 0.050 m to 0.161 m in the vertical coordinates while the mean errors in the structure's edges were 0.068 m and 0.071 m in horizontal and vertical coordinates, respectively. Therefore, this study confirmed the potential of 3D models from UAV photogrammetry for analyzing the digital twin and slope as well as BIM (Building Information Modeling).

• Journal of Korean Society for Geospatial Information Science
• /
• v.23 no.4
• /
• pp.25-33
• /
• 2015

The study of ecosystem assessment(ES) is based on land cover information, and primarily it is performed at the global scale. However, these results as data for decision making have a limitation at the aspects of range and scale to solve the regional issue. Although the Ministry of Environment provides available land cover data at the regional scale, it is also restricted in use due to the intrinsic limitation of on screen digitizing method and temporal and spatial difference. This study of objective is to generate UAV land cover map. In order to classify the imagery, we have performed resampling at 5m resolution using UAV imagery. The results of object-based image segmentation showed that scale 20 and merge 34 were the optimum weight values for UAV imagery. In the case of RapidEye imagery;we found that the weight values;scale 30 and merge 30 were the most appropriate at the level of land cover classes for sub-category. We generated land cover imagery using example-based classification method and analyzed the accuracy using stratified random sampling. The results show that the overall accuracies of RapidEye and UAV classification imagery are each 90% and 91%.

• Science of Emotion and Sensibility
• /
• v.10 no.4
• /
• pp.539-554
• /
• 2007

Pilots of large airplanes have to land their airplanes with insufficient visual information because of high approach speed, high vertical velocity and high location or altitude of the cockpits from the runway intending to touch down. This study verifies that, due to the insufficient information, large airplane pilots can't exactly perceive height of their airplanes during the flare. Study 1 explored whether it's possible for the pilots to accurately perceive height with the static visual cues only. We showed them pictures of the runway taken from the pilot's pionts of view and asked them to assess the height of the airplanes. They determined exact height of the airplanes at the height of 85 feet, but they could not, at lower than 55 feet which is the flare preparation altitude. Study 2 explored whether it's possible for the pilots to accurately perceive height when dynamic cues were added to the static visual cues. We showed them videos of the runway taken from the pilot's pionts of view. With more cues they determined exact height of the airplanes at the height of 50 feet, but they could not, at the altitude of lower than 30 feet which is the flare altitude. As experience is believed to be a major factor which affects interpretation of the visual cues, we compared the accuracy of the assessment of the experienced captions and that of the in-experienced first officers. We found there was no significant difference between them.

• Journal of the Korean Society of Radiology
• /
• v.12 no.2
• /
• pp.139-148
• /
• 2018

Assessment of NPP(Nuclear Power Plant) decommissioning cost is very important for safe decommissioning of nuclear power plants. In the United States, which has the most NPP decommissioning experience, the cost evaluation study has been conducted since the 1970s in order to decommissioning nuclear facilities. The US NRC has conducted studies on decommissioning technology, safety and cost for a variety of reactor type and nuclear installations. In the total decommissioning costs, the end of operation licenses accounted for the largest portion, followed by spent fuel management and site restoration. In case of immediate decommissioning, spent fuel management cost increased compared to delayed decommissioning, and delayed deocmmissioning increased the cost of terminating the operation license. However, in general, delayed decommissioning does not show any significant benefit as compared with immediate decommissioning. It is necessary to consider the evaluation according to the site conditions when evaluating the cost of decommissioning domestic nuclear power plants. Also, in Korea, IAEA recommendations were applied to reorganize the radioactive waste classification system. Therefore, it is necessary to develop a method to appropriately use the decommissioning data of the preceding US Nuclear Power Plant in the new classification system when estimating the amount of radioactive waste generated during decommissioning. In particular, the establishment of the evaluation methodology for the waste to be disposed of will be an important factor in securing the accuracy of the decommissioning cost. In addition, it is necessary to construct information data that can be applied to facility characteristics and work characteristics in order to evaluate the cost of demolition of domestic nuclear power plants.

• The Korean Journal of Nuclear Medicine
• /
• v.25 no.1
• /
• pp.37-45
• /
• 1991

Infarct size is a major determinant of prognosis after acute myocardial infarction. Up to date, however, clinically available tests to estimate this size have not been sufficiently accurate. Twelve lead electrocardiogram and wall motion abnormality measurement are not quantitative, and creatine phophokinase (CPK) measurement is inaccurate in the presence of reperfusion or right ventricular infarction. Methods have been developed to localize and size acute myocardial infarcts with agents that are selectively sequestered in areas of myocardial damage, but previously used agents have lacked sufficient specificity. Antibodies that bind specifically only to damaged myocardial cells may resolve this problem and provide an accurate method for noninvasively measuring infarct size. We determined the accuracy with which infarcted myocardial mass can be measured using single photon emission computed tomography (SPECT) and radiolabeled antimyosin antibodies. Seven patients with acute myocardial infarction and one stable angina patient were injected with 2 mCi of Indium-111 labeled antimyosin antibodies. Planar image and SPECT was performed 24 hours later. None of the patients had history of prior infarcts, and none had undergone reperfusion techniques prior to the study, which was done within 4 days of the attack. Planar image showed all infarct patients to have postive uptakes in the cardiac region. The location of this uptake correlated to the infarct site as indicated by electrocardiography in most of the cases. The angina patient, however, showed no such abnormal uptake. Infarct size was determined from transverse slices of the SPECT image using a 45% threshold value obtained from a phantom study. Measured infarct size ranged from 40 to 192 gr. There was significant correlation between the infarct size measured by SPECT and that estimated from serial measurements of CPK (r=0.73, p<0.05). These date suggest that acute myocardial infarct size can be accurately measured from SPECT Indium-111 antimyosin imaging. This method may be especially valuable in situations where other methods are unreliable, such as early reperfusion technique, right ventricular infarct or presence of prior infarcts.

• The Korean Journal of Nuclear Medicine
• /
• v.33 no.1
• /
• pp.57-64
• /
• 1999

Purpose: The purpose of this study was to evaluate the accuracy of Tc-99m DTPA diuretic renal scans in children with dilated upper urinary tract. Materials and Methods: We reviewed diuretic renal scans of 14 pediatric patients (age range: 3 days to 4 years) with unilateral hydronephrosis diagnosed by ultrasonography. Diuretic renal scan was done using Tc-99m DTPA and standardized protocol. In 3 neonates, diuretic renal scans were performed within 1 week and 3-7 months after birth. Results: Six patients required Pyeloplasty and eight were managed conservatively. All 6 patients requiring Pyeloplasty were diagnosed as having ureteropelvic junction obstruction in the diuretic renal scan. In these 6 patients, post-operative renal scans at 3-12 months after surgery were converted to nonobstructive pattern in 5 and a nonfunctioning pattern in 1. In 3 patients who underwent diuretic renal scan within 1 week after birth, nonobstructive patterns of initial scan were converted to obstructive patterns in the follow-up scan. However, all patients with nonobstructive diuretic renal scans performed after the neonatal period did well on serial ultrasonography and showed favorable clinical outcome without progression to obstruction. Conclusion: Tc-99m DTPA diuretic renal scan with standardized protocol is useful in assessing suspected ureteropelvic junction obstruction in children as an initial diagnostic or post-operative follow-up modality. Nonobstructive or indeterminate scan results in the neonatal period requires follow-up scan to monitor development of the obstructive pattern.

• Korean Journal of Clinical Laboratory Science
• /
• v.49 no.4
• /
• pp.366-373
• /
• 2017

Urinalysis is considered to be easier and simpler than other tests. It has been known to cause no burden to patients, while offering important information on diagnosing, treating, and determining the prognoses of kidney and urinary tract diseases. Urinary sediments are usually performed by microscopic examination of centrifuged urine by technologist. The guidelines proposed by the Korean Association of External Quality Assessment Service are actually different from those actually practiced by medical institutions and taught to biomedical students in textbooks. Therefore, we verified whether different sediment preparation methods lead different test results. Specimens that tested positive from the occult blood and leukocyte esterase in the urine dipstick test were randomly selected for a microscopic examination. The differences in the urine sediment preparation affected the sediment concentrations, which influenced the cell grade and cell number per HPF. The first factor in determining the sediment concentration is the centrifugal force. Many medical institutions use 1,500 rpm as the centrifugal speed without considering the radius of the centrifuge; such a value may not be accurate for 400 G. Consequently, there were differences in urine concentrations, which influenced the results. The second factor is the amount of sediment in urine. Different amounts of the remaining supernatant led to different sediment concentration factors, again, causing different results. Furthermore, not only by using a pipette to obtain an accurate amount as stipulated, but also by roughly obtaining a drop, the microscopic examination using such a volume of sediment examined affected the results. Therefore, this study highlights the importance of standardization of urine sediment preparation procedures to promote consistency and accuracy across institutions.