• Journal of the Optical Society of Korea
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• 제18권4호
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• pp.401-405
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• 2014

In this paper, we propose an accumulation encoding scheme based on double random phase encryption (DRPE) for multiple-image transmission. The proposed scheme can be used for a low-complexity DRPE system due to the simple structure of the accumulation encoder and decoder. For accumulation encoding of multiple images, all of the previously encrypted data are added, and hence the accumulation encoding can improve the security of the DRPE-encrypted data. We present a scheme for encryption and decryption for DRPE-based accumulation encoding, and a method for accumulation encoding and decoding. Finally, simulation results verify that the DRPE-based accumulation encoding scheme for multiple images is powerful in terms of data security.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.531-534
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• 2000

The fumB gene encoding anaerobic fumarase of Escherichia coli XL1-Blue was introduced to solve the malic acid accumulation problem. When NZN111 harboring pTrcMLFu was cultured, 7 g/L of succinic acid was produced and malic acid was not accumulated.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.234-234
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• 2018

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2002년도 봄 학술발표대회 발표논문집
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• pp.209-212
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• 2002

본 연구는 방향족 화합물질 중 페놀폐수에 대한 생물학적 처리를 위해 본 실험실에서 분리한 페놀분해능이 우수한 Rhodococrus sp. EL-GT를 이용하여 catechol 분해 catechol 1,2-dioxygenase 분해활성을 측정하였고, 이것이 ortho-pathway임을 확인할 수 있었다. 또한 다른 연구에서 보고된 Rhodococcus rhodochrous NCIMB 13259 균주의 catechol 1,2 dioxygenase를 기초로한 primer를 이용하여 PCR을 수행하였으며 이 분해 유전자의 cloning실험을 수행 중이다. 이들 실험을 통하여 Rhodococcus sp.의 페놀분해균의 유전적 구조 및 특성을 검토하고 밝혀지는 단백질 정보를 이용하여 방향족 화합물의 분해능이 보다 우수한 균주의 개발을 시도하고자 한다.

• Plant Resources
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• 제1권1호
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• pp.13-21
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• 1998

A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

• Journal of Microbiology and Biotechnology
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• 제23권3호
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• pp.304-312
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• 2013

The thermotolerant methylotrophic yeast Hansenula polymorpha is attracting interest as a potential strain for the production of recombinant proteins and biofuels. However, only limited numbers of genome engineering tools are currently available for H. polymorpha. In the present study, we identified the HpPOL3 gene encoding the catalytic subunit of DNA polymerase ${\delta}$ of H. polymorpha and mutated the sequence encoding conserved amino acid residues that are important for its proofreading 3'${\rightarrow}$5' exonuclease activity. The resulting $HpPOL3^*$ gene encoding the error-prone proofreading-deficient DNA polymerase ${\delta}$ was cloned under a methanol oxidase promoter to construct the mutator plasmid pHIF8, which also contains additional elements for site-specific chromosomal integration, selection, and excision. In a H. polymorpha mutator strain chromosomally integrated with pHIF8, a $URA3^-$ mutant resistant to 5-fluoroorotic acid was generated at a 50-fold higher frequency than in the wild-type strain, due to the dominant negative expression of $HpPOL3^*$. Moreover, after obtaining the desired mutant, the mutator allele was readily removed from the chromosome by homologous recombination to avoid the uncontrolled accumulation of additional mutations. Our mutator system, which depends on the accumulation of random mutations that are incorporated during DNA replication, will be useful to generate strains with mutant phenotypes, especially those related to unknown or multiple genes on the chromosome.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.71-77
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• 2010

The time, yield, and related genes expression of PHB accumulation of Acidiphilium cryptum DX1-1 were investigated under four different initial C/N ratios, 1.2, 2.4, 7.5, and 24. The results of time and yield of poly-$\beta$-hydroxybutyrate (PHB) accumulation show that the initial C/N ratio of 2.4 was optimum for strain DX1-1 to accumulate PHB, but both higher and lower initial C/N ratios did not favor that process. Based on the genome of Acidiphilium cryptum JF-5, 13 PHB accumulation related genes in strain JF-5 were chosen and successfully cloned from strain DX1-1. The differential expressions of the 13 functional genes, in different C/N ratios as cited above, were then studied by real-time PCR. The results show that all the 13 genes were most upregulated when the initial C/N ratio was 2.4, and among which the gene Acry_3030 encoding poly-$\beta$-hydroxybutyrate polymerase and Aery_0626 encoding acetyl-CoA synthetase were much more upregulated than the other genes, which proved that they play the most important role for PHB accumulation, and acetate is the main initial substance for PHB accumulation for strain DX1-1. Potential regulatory motifs analysis showed that the genes related to PHB accumulation are regulated by different promoters and that the motif had weak similarity to the model promoters, suggesting that PHB metabolism in Acidiphilium cryptum may be mediated by a different mechanism.

• BMB Reports
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• 제42권1호
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• pp.28-34
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• 2009

To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding ${\Delta}^1$-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine $\delta$-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development.

• JSTS:Journal of Semiconductor Technology and Science
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• 제14권4호
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• pp.419-426
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• 2014

This paper proposes a high-throughput encoding process and encoder architecture for quasi-cyclic low-density parity-check codes in IEEE 802.11ac standard. In order to achieve the high throughput with low complexity, a partially parallel processing based encoding process and encoder architecture are proposed. Forward and backward accumulations are performed in one clock cycle to increase the encoding throughput. A low complexity cyclic shifter is also proposed to minimize the hardware overhead of combinational logic in the encoder architecture. In IEEE 802.11ac systems, the proposed encoder is rate compatible to support various code rates and codeword block lengths. The proposed encoder is implemented with 130-nm CMOS technology. For (1944, 1620) irregular code, 7.7 Gbps throughput is achieved at 100 MHz clock frequency. The gate count of the proposed encoder core is about 96 K.

• 생명과학회지
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• 제17권5호
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• pp.714-718
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• 2007

유전자의 구조 예측 및 발현 기작에 대한 연구는 매우 중요한 사안으로 대두되고 있다. 특히 유전자 발현 제어에 중요한 역할을 하는 프로모터 영역을 예측하는 것은 전체 생명체 네트워크 규명을 위한 단초를 제공하기 때문에 많은 연구가 이루어지고 있다. 본 논문에서는 이러한 진핵생물의 유전자 프로모터 예측을 위한 Support Vector Machine(SVM) 활용방안에 대한 연구내용을 다루고 있다. 특성 벡터 값 생성을 위한 인코딩 방법 및 학습 데이터들의 구성에 대한 다양한 실험을 통해 SVM활용 방안에 대한 올바른 방향을 제시하고 있다.