• Title/Summary/Keyword: Acclimation Period

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Body Weight Changes of Laboratory Animals during Transportation

  • Lee, Sung-Hak;Nam, Hyun-Sik;Kim, Jin-Sung;Cho, Hye-Jung;Jang, Yu-Mi;Lee, Eun-Jung;Choi, Eun-Sung;Jin, Dong-Il;Moon, Hong-Sik
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.286-290
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    • 2012
  • The majority of laboratory animals were transported from commercial breeders to a research facility by ground transportation. During the transportation, many biological functions and systems can be affected by stress. In this experiment, the change of body weight during the transportation was measured and the recovery periods from the transportation stress established based on the body weight changes. Total 676 laboratory animals which were aged between 3 to 9 wk old were studied. The transportation time taken from container packing to unpacking the container was approximately 24 h. The temperature of animal container was constantly maintained by air-conditioning and heating equipment. Rats were found to be more sensitive than mice. The body weight of rats was significantly decreased 3.71% (p<0.05) compared to the body weight of mice which decreased 0.9% There was no significant difference between the strains in the same species. When the changes of body weights were compared between delivery days, C57BL/6 mice showed the most variable changes compared to other species and strains. Consequently, C57BL/6 was more sensitive to stress than the other strains and the transportation process needs to be standardized to reduce between day variability. To establish the recovery periods from transportation stress, the body weight changes were measured during the acclimation period. Although the body weight of animals decreased during transportation, animals recovered their weight loss after the next day.

Study on Rate-Limiting Factors with a Heavy Loaded Biofilter

  • Son, Hyun-Keun
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.E2
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    • pp.74-81
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    • 2007
  • Feasibility test for a biofilter was performed to treat VOCs. The applied loading rate to the biofilter was calculated between 60 to $3,700\;kg\;COD/m^3$. Trimethyl-pentene and trimethyl-hexene were the two most dominant compounds and they occupy about 85 percent. During the acclimation period, it is desirable for a biofilter to receive relatively lower VOCs concentration and flow rate, until it can adjust to new substrate and operational environment. Temperature at various points inside the biofilter reactor was observed with more than 23 temperature sensors. With steam heating, temperatures of the top sections of the media were greater than those of bottom sections. Without steam heating, intermediate stages generally had higher temperature measurement than those of bottom and top stages. Because the pH values for different biofilter materials vary significantly, measurement of the pH for the mixture of different combinations of biofilter materials is necessary. Based on the types and brands of media, the measured pH ranged from 5.38 to 9.10. The range of measured pH of different mixtures with perlite, compost, saw dust, peat moss, limestone, vermiculite was 7.05 to 8.62.

The Reproductive Characteristics of the Mare in Subtropical Taiwan

  • Ju, Jyh-Cherng;Peh, Huo-Cheng;Hsu, Jenn-Chung;Cheng, San-Pao;Chiu, Shaw-Ching;Fan, Yang-Kwang
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.4
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    • pp.494-499
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    • 2002
  • The objectives of this study were to document the reproductive traits of mares as influenced by the month of the year in Taiwan. Reproductive records, lactation traits, foal birth weight (FBW) and foal height (FBH) were collected from Holi Equine Station of Taiwan. The effects of month on these parameters were analyzed. The length of estrus (LE) was shortest in December each year. The increasing trend was recorded from January to September with a significantly (p<0.05) longer period of $12.4{\pm}0.4$ days in September than in January and February. A gradual shortening in LE was observed from September to December ($10.1{\pm}0.6$ days, p<0.05), when the shortest period of the year was observed. Mares showed signs of estrus throughout the year, but more than 80% were found in estrus during March through October. The FBW was significantly (p<0.05) affected by the breeding month of the year. The lowest foal weights were recorded in both September ($36.7{\pm}0.7$ kg) and December ($36.8{\pm}0.9$ kg), which were also significantly lower than those in other months except in March, August, and November. A trend of lower FBH from September to December (93.5-93.8 cm) than those from January to August was observed. The greatest FBH was in June (96.2 cm). Breeding months and onset of estrus of the mares exerted a significant effect on the incidence of agalactia during the lactation period. These analyses provide fundamental information on adaptive processes in respect to reproductive characteristics of mares, which indicated an extent of acclimation by these animals in subtropical Taiwan.

Use of Dietary Salt to Rainbow Trout (Oncorhynchus mykiss) for Increasing Seawater Adaptability (무지개송어(Onchorhynchus mykiss)의 해수 적응 능력 개선을 위한 식염사료의 적용)

  • Kim, Pyong-Kih;Kim, You-Hee;Jeon, Joong-Kyun
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.69-75
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    • 2005
  • One 30-day feeding trial was conducted to examine the effects of dietary salt on seawater adaptability of rainbow trout (Onchorhynchus mykiss) fed three experimental diets containing 0% (control), 4% and 8% salt. The experimetal period included 30 days of feeding trial in freshwater, 3 days of the step by step seawater acclimation with-out feeding diets, and 21 more days of seawater adaptation period (not with all experimental fish) with feeding the basal diet. Growth rates from triplicate groups were determined fur 30 days of feeding trial. Blood samples were taken at the begining and at the end of feeding trial, and 3 times (on 1st, 4th and 8th day) of the seawater adaptation period. Daily survival rates of duplicate groups from three experimetal treatments were recorded for 21 days of the seawater adaptation period. Total average initial and final fish weight were $149.5{\pm}7.6\;and\;187.1{\pm}7.6g$. Feed efficiency of fish fed diets containing 4% and 8% salt were significantly better than those of fish fed the control diet. Average cumulative survival rates were 72, 80 and 88% from the control, 4% and 8% salt diets, respectively. Pulse rate per minutes decreased with dietary salt level. Serum $Na^+\;and\;Cl^-$ concentrations of fish fed 4% and 8% salt diets were significantly higher than those of fish fed the control diet (P<0.05), however, the concentrations were stabilized after 8 days of seawater adaptation. Serum cortisol, glucose, cholesterol and tryglyceride concentrations, and the osmorality of fish decreased with dietary salt level, these values were significantly lower than those of fish fed the control diet. These results indicated that the dietary supplementation of salt could have advantages for seawater adaptability of rainbow trout.

Effects of liposomal-curcumin on five opportunistic bacterial strains found in the equine hindgut - preliminary study

  • Bland, S.D.;Venable, E.B.;McPherson, J.L.;Atkinson, R.L.
    • Journal of Animal Science and Technology
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    • v.59 no.6
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    • pp.15.1-15.5
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    • 2017
  • Background: The horse intestinal tract is sensitive and contains a highly complex microbial population. A shift in the microbial population can lead to various issues such as inflammation and colic. The use of nutraceuticals in the equine industry is on the rise and curcumin is thought to possess antimicrobial properties that may help to minimize the proliferation of opportunistic bacteria. Methods: Four cecally-cannulated horses were utilized to determine the optimal dose of liposomal-curcumin (LIPC) on reducing Streptococcus bovis/equinus complex (SBEC), Escherichia coli K-12, Escherichia coli general, Clostridium difficile, and Clostridium perfringens in the equine hindgut without adversely affecting cecal characteristics. In the first study cecal fluid was collected from each horse and composited for an in vitro, 24 h batch culture to examine LIPC at four different dosages (15, 20, 25, and 30 g) in a completely randomized design. A subsequent in vivo $4{\times}4$ Latin square design study was conducted to evaluate no LIPC (control, CON) or LIPC dosed at 15, 25, and 35 g per day (dosages determined from in vitro results) for 9 days on the efficacy of LIPC on selected bacterial strains, pH, and volatile fatty acids. Each period was 14 days with 9 d for acclimation and 5 d withdrawal period. Results: In the in vitro study dosage had no effect ($P{\geq}0.42$) on Clostridium strains, but as the dose increased SBEC concentrations increased (P = 0.001). Concentrations of the E. coli strain varied with dose. In vivo, LIPC's antimicrobial properties, at 15 g, significantly decreased (P = 0.02) SBEC when compared to 25 and 35 g dosages. C. perfringens decreased linearly (P = 0.03) as LIPC dose increased. Butyrate decreased linearly (P = 0.01) as LIPC dose increased. Conclusion: Further studies should be conducted with a longer dosing period to examine the antimicrobial properties of curcumin without adversely affecting cecal characteristics.

Effects of Low Salinity acclimation on Oxygen Consumption in Giant Grouper, Epinephelus lanceolatus (염분변화가 대왕바리, Epinephelus lanceolatus의 산소소비에 미치는 영향)

  • Kim, Moon-Jin;Lim, Han-Kyu;Jeong, Min-Hwan
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.2
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    • pp.526-536
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    • 2015
  • Changes of oxygen consumption in juvenile giant grouper, Epinephelus lanceolatus were investigated in order to find out the physiological responses associated with freshwater and low salinities acclimation. Salinity changes in this experiment were set as follows; 35 psu (Exp. I) as control, decrease in the manners of $35{\rightarrow}20{\rightarrow}10{\rightarrow}0$ psu (Exp. II), $35{\rightarrow}10{\rightarrow}0$ psu (Exp. III) and $35{\rightarrow}0$ psu (Exp. IV). The average oxygen consumption at $27^{\circ}C$ in Exp. I (control) was $106.9{\pm}0.7mg$ $O_2/kg/h$. In Exp. II, according to the changes of $35{\rightarrow}20{\rightarrow}10{\rightarrow}0$ psu in freshwater acclimation, fish showed the gradual decrease in oxygen consumption as $108.1{\pm}2.1$, $99.6{\pm}2.9$, $74.6{\pm}0.9$ and $62.7{\pm}1.0mg$ $O_2/kg/h$, respectively. The fish in Exp. III also revealed the same tendency as $106.5{\pm}1.3$, $77.0{\pm}1.2$ and $64.7{\pm}1.2mg$ $O_2/kg/h$, respectively. In Exp. IV, fish showed the decreased oxygen consumption from $109.0{\pm}0.9mg$ $O_2/kg/h$ of 35 psu to $71.6{\pm}2.0mg$ $O_2/kg/h$ of 0 psu. Giant grouper is thought to be a diurnal fish species, because the level of oxygen consumption during light period was higher than that during dark period.

Evaluation on the Biodegradability of the MBT Wastewater (MBT 폐수의 생분해성 평가)

  • Lim, Ji-Young;Park, Jung-hwan;Kim, Jin-Han
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.3
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    • pp.86-92
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    • 2016
  • The possibility of the biological treatment of MBT wastewater generated from the vulcanization accelerator manufacturing process was investigated. MBT wastewater is not biodegradable because it hinders the activity of microorganisms, and approximately 10% of the total COD can be removed after a 7 day acclimation period. The optimal conditions of the MBT wastewater for the chemical pre-treatment was pH of 3.5 and the Fenton oxidation with the addition of $Fe^{3+}$ to the wastewater after agitation for 2 hours. The Fenton-treated MBT wastewater showed approximately 20% removal of COD when treated with the activated sludge process for the mixed paper wastewater and Fenton treated wastewater.

Characteristics of daily mortality due to heat waves in Busan in July 1994 (1994년 7월 부산지역의 폭염으로 인한 일 사망률 특성 연구)

  • Lee, Dae-Geun;Kim, Jiyoung;Cho, Byoung-Cheol
    • Atmosphere
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    • v.17 no.4
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    • pp.463-470
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    • 2007
  • This study aims to assess the impact of the hot summer weather on daily mortality in Busan. Daily total all-caused mortality in the entire population in Busan has been examined during 1991-2005. The daily deaths were standardized to account for the long-term trend in mortality and their seasonal and weekly cycles. We found the net increase (about 8.2%) of excess deaths during the extraordinary heat wave period in July of 1994. It corresponds to the excess deaths of 109.5 during the month. The abnormality of temperature extremes in July of 1994 and their impacts on human health were also investigated. Unusual heat wave appeared in the first ten days in July of 1994. The excess deaths are likely to be attributable to the record-breaking heat waves. The result suggests that unusual early heat waves would be dangerous, even for inhabitants who live in an acclimated region to the heat waves such as Busan.

Comparison of Ammonia-Oxidizing Bacterial Community Structure in Membrane-Assisted Bioreactors Using PCR-DGGE and FISH

  • Ziembinska, A.;Ciesielski, S.;Gnida, A.;Zabczynski, S.;Surmacz-Gorska, J.;Miksch, K.
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1035-1043
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    • 2012
  • The ammonia-oxidizing bacterial (AOB) communities in three membrane bioreactors (MBRs) were monitored for 2 months after an acclimation period in order to investigate the influence of sludge age and medium type on AOB changeability and its connection with nitrification effectiveness. One MBR with a sludge age of 4 days was fed with a synthetic medium, whereas the other two with sludge ages of 8 and 32 days were fed with landfill leachate. The research revealed that landfill leachate can be effectively treated in an MBR with a higher sludge age for longer periods of time and that this improvement in performance was correlated with an increase in AOB biodiversity. Interestingly, the medium type has a stronger influence on AOB biocenosis formation than the sludge age.

Critical Thermal Maximum (CTM) of Cultured Black Rockfish, Sebastes schlegeli

  • Kim Wan-Soo;Yoon Seong-Jin;Gil Joon-Woo
    • Fisheries and Aquatic Sciences
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    • v.6 no.2
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    • pp.59-65
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    • 2003
  • The critical thermal maximum (CTM) of black rockfish, Sebastes schlegeli, was evaluated. Black rockfish were acclimated at $24^{\circ}C$, and then exposed to temperatures from 24 to $33^{\circ}C$. Black rockfish were kept in constant darkness and subjected to a gradual temperature increase $(1 ^{\circ}C\;12^{-1})$. The oxygen consumption rate (OCR) was measured using an automatic intermittent­flow-respirometer (AIFR) during the exposure period (from 119.3 to 143.5 h). The OCR increased from 94.5 to 214.2mL $O_2 kg^{-1}\;ww\;h^{-1}$ as the temperature rose from 24 to $29.4-30.9^{\circ}C$. Subsequently, the OCR increased abruptly, reaching 245.8-412.7mL $O_2 kg^{-1}\;ww\;h^{-1}$ at $32^{\circ}C$. This study suggests that the CTM for black rockfish is $29.4-30.9^{\circ}C$ when temperature is increased at $1^{\circ}C\;12h^{-1}$ following acclimation at $24^{\circ}C$.