• Title/Summary/Keyword: Acclimation

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Proton Effect on the Degradation of Phenolic Compound by Activated Sludge and Nocardia asteroides (활성슬러지 혼합미생물과 Nocardia asteroides에 의한 페놀화합물 분해시 양성자이온의 영향)

  • 조관형;조영태;우달식
    • Journal of Environmental Science International
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    • v.11 no.6
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    • pp.561-567
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    • 2002
  • This study was investigated to evaluate the effect of the sodium ion and pH on toxicity of dinitrophenol at high concentrations (0.41 to 0.54 mM), over a sodium concentration range of 0.1 mM to 107 mM and over a pH range of 5 to 9. The concentration of sodium ions in the activated sludge mixed liquor seemed to have very little effect on dinitrophenol toxicity. However, lack of sodium in the growth media resulted in a reduction of the dinitrophenol degradation rate by bacterial isolate from the activated sludge culture, which has been identified as Nocardia asteroides. Dinitrophenol inhibition was found to be strongly dependent on mixed liquor pH. The dinitrophenol degradation rate was highest in the pH range of 6.95 to 7.84; at pH 5.94 degradation of 75 mg/L dinitrophenol was significantly inhibited; at pH < 5.77, dinitrophenol degradation was completely inhibited after approximately 30% of the dinitrophenol was degraded.

Role of Sodium lon in Biodegradation of Nitroaromatic Compound by Activated Sludge and Pure Cultures

  • Jo, Kwan-Hyung
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.3 no.3
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    • pp.169-175
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    • 1999
  • 2,4-Dinitrophenol(DNP) is a metabolic uncoupler that prevents cells from creating energy for growth and it has been suggested that the availability of sodium ions may be important in mitigating the effects of uncouplers. Accordingly, the degradation of DNP was investigated using activated sludge which had been adapted to mineralize DNP. After the acclimation of the activated sludge, the effect of sodium ions on the toxicity of high concentrations(80 to 100mg/L) of DNP was investigated over a sodium ion concentration range of 9.3$\times$10-5 to 94mM. The concentration of sodium ions in the activated sludge mixed liquor seemed to have little effect on the DNP toxicity. However, a lack of sodium in the grwoth media resulted in a reduction of the DNP degradation rate by a bacterial isolate from the activated sludge culture identified as Nocardia asteroides.

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Screening of Potential Stress-Responsive and Immune-Related Genes by Expressed Sequence Tags in Mud Loach (Misgurnus mizolepis)

  • Nam, Yoon-Kwon;Kim, Dong-Soo
    • Journal of fish pathology
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    • v.15 no.2
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    • pp.83-92
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    • 2002
  • EST analysis was performed to identify stress-responsive and immune-related genes from mud loach (Misgurnus mizolepis), cDNA libraries were constructed with liver, intestine and kidney tissues and randomly chosen clones (216 for liver, 198 for intestine and 224 for kidney) were subjected to automated sequence analysis. Of 638 clones sequenced in totlal, approximalely 25% of ESTs was novel sequences (no match to GenBank) or sequences with high homology to hypothrtical/unknown genes. Several potential stress-responsive biomarker and/or immure-related genes were identified in all the tissues examined. It included lectin, MHC class I/II proteins, proteinase inhibitors, superoxide dismulase, catalase, glutathionc-S. transferase, heat-shock protein, warm temperature acclimation protein, complements, methylrransferasc, zinc finger proteins, macrophage maturation associated protein, and others. This information will offer new possibilities as fundamental baseline data for the molecular genetics and breeding of this species with an emphasis on the development of stress. (and disease)-resistsnt fish.

Comparison of structure, function and regulation of plant cold shock domain proteins to bacterial and animal cold shock domain proteins

  • Chaikam, Vijay;Karlson, Dale T.
    • BMB Reports
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    • v.43 no.1
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    • pp.1-8
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    • 2010
  • The cold shock domain (CSD) is among the most ancient and well conserved nucleic acid binding domains from bacteria to higher animals and plants. The CSD facilitates binding to RNA, ssDNA and dsDNA and most functions attributed to cold shock domain proteins are mediated by this nucleic acid binding activity. In prokaryotes, cold shock domain proteins only contain a single CSD and are termed cold shock proteins (Csps). In animal model systems, various auxiliary domains are present in addition to the CSD and are commonly named Y-box proteins. Similar to animal CSPs, plant CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. Cold shock domain proteins have been shown to play important roles in development and stress adaptation in wide variety of organisms. In this review, the structure, function and regulation of plant CSPs are compared and contrasted to the characteristics of bacterial and animal CSPs.

The Study of Microbial Population & Dynamics in Hydrocarbon Contaminated Areas (유류오염지역의 미생물 분포 및 활성도에 관한 연구)

  • 김무훈;김순기;이원권;경우성;박덕신
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1999.10a
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    • pp.28-31
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    • 1999
  • The purpose of this study is to see the effect of microbial population and dynamics of the indigeonous microorganisms on hydrocarbon contaminated areas. The microbial structures and activities to determine the microbial capabilities of the contaminated sites are very important for the remedial action technology selection. Throughout microbial studies on different conditions by ETS(Electron Transport System) and microbial activity analysis, it was found that aeration and water contents are the most important factors in this site remediation. According to test results, Burkholderia spp. was dominant species, and acclimation is also an important factor for the accerelated biodegradation.

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Application of in vitro ovary culture for cottonwood (Populus deltoides) breeding

  • Kang, Ho-Duck;Lee, Min-Soon
    • Plant Resources
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    • v.7 no.2
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    • pp.87-92
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    • 2004
  • Five different poplar hybrids were tested for rescuing embryo to elongate in vitro plantiets after hybridization. Ovaries and ovules were cultured on Woody Plant Medium (WPM) supplemented with cytokinins, 6-benzylamine (BA) and zeatin. Multiple shoots were initiated from half section of capsule with immature embryos after 21 days from pollination and tiny shoots were formed after the expansion of cotyledons in ovule cultures. Germinating response was better in intraspecific hybrids $(6.53\pm1.66)$ than interspecific crosses $(0.93\pm0.54)$ from half section of capsules on WPM medium. In general, zeatin was better than BA in inducing multiple shoots from isolated ovules. The highest average number $(19.40\pm4.53)$ of shoots was produced from immature ovules of 21 days post-pollination of WPM medium supplemented with 5.0 mg/L zeatin. The highest percentage of germination was 93% from the half section of in vitro ovary cultures. Soil acclimation was successfully conducted in cell tray containing artificially mixed soil with 96% survival rate.

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Establishment and Using of mass propagation system for Calanthe discolor Lindle (새우난초(Calanthe discolor Lindley)의 대량증식 시스템 확립 및 이용)

  • 서병기
    • Korean Journal of Plant Resources
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    • v.15 no.3
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    • pp.221-226
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    • 2002
  • This experiment was conducted to establish the mass propagation system of Calanthe discolor Lindley. When the Calanthe discolor seeds were sown in Murashige and Skoog medium, the percentage of germination was 65%. Seedlings grew more rapidly in the liquid medium than the solid medium. All regenerated plantlets were survived in acclimatized condition of 70% shade and more than 80% humidity. Also, we found out that the 88% of survival ratio could be achieved in containing soil mixture of vermiculite and perlite as same as amount.