• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.438-447
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• 2017

Flowering is one of the most important development traits related to the production of Brassica rapa crops. After planting, a sudden low temperature triggers premature flowering, which leads to a reduction in the yield and quality of harvested production. Therefore, understanding the mechanism of flowering control is important in the agricultural productivity for preventing Brassica rapa crops. Vernalization is generally known as the main factor of flowering in the Brassica plant. However, in the subspecies of Brassica rapa, some accession such as Yellow sarson and Komatsuna display the flowering phenotype without vernalization. Circadian genes, which diurnally regulate plant physiology, have a role for photoperiodic flowering but are related to the regulation of the vernalizarion mechanism. In this report, the 22 B. rapa accession were divided into two groups, vernalization and non-vernalization, and the sequenced circadian gene, BrPRR1s. Among them, the BrPRR1b gene was found to have deletion regions, which could classify the two groups. The PCR primer was designed to amplify a short band of 422bp in the vernalization type and a long band of 451bp in the non-vernalization type. This primer set was applied to distinguish the flowering types in the 43 B. rapa accession and 4 Brassica genus crop, Broccoli, cabbage, mustard, and rape. The PCR analysis results and flowering time information of each crop demonstrated that the primer set can be used as marker to discern the flowering type in Brassica crops. This marker system can be applied to the B. rapa breeding when selecting the flowering character of new progenies or introducing varieties at an early stage. In addition, these results displayed that the circadian clock genes can be a good strategy for the flowering control of B. rapa crops.

• Korean Journal of Food Science and Technology
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• v.45 no.1
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• pp.1-7
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• 2013

Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus are involved in the Perciformes Order and Serranidae Family. When E. bruneus and E. septemfasciatus are fully grown, the striped pattern on the body gradually disappears. Therefore, morphological classification of adult fishes is quite difficult to identify the differences to N. spinosus. In this study, we investigate the method to differentiate those using PCR. To design the primers, 16S rRNA region of N. spinosus, E. bruneus, and E. septemfasciatus registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for the analysis, Bio Edit ver. 7.0.9.0 was used. As a result, it was design NS-003-F/NS-005-R (136 bp), EB-001-F/EB-002-R (181 bp), and ES-001-F/ES-001-R (123 bp) primers for the differentiation of each 3 different fishes. Therefore, the species-specific primer sets would be a useful tool for scientific and speedy differentiation against the illegal distribution for consumer protection.

• Journal of the Korean Society of Food Culture
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• v.30 no.2
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• pp.150-157
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• 2015

GichukJinchanEuigwe is a celebration of King Soonjo's 40th birthday and 30th accession to the throne. The Royal banquet was carried out on February 9th MunggunggunJinchan and JagyeongjeonJeongilJinchan 3 days later. JagyeongjeonJeongilJinbyulhangua is located beside JagyeongjeonJinchanan. The banquet table setting, table, dish level, and table type are different according to royal hierarchy class. Jinbulhangua is served to only the king, queen, crown prince, crown princess, and Myongon princess. The number of JagyeongjeonJinchanan served was as follows: king 30 plates, crown prince 20 plates, and Myongon princess 15 plates. Tableware used were brassware and pottery.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.230-234
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• 2006

Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in 'Sinma'; and chlorotic spots, necrosis of axillary shoots and withering of leaves in 'Hwarang'. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.

• Journal of the Korean Society for information Management
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• v.11 no.1
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• pp.167-187
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• 1994

This project is to design and to implement an cornputenzed acquisition system by structured analysis technique for the Yomi university library. In result of desgmng, we added three subsystems, whlch are accession control, account, and print out system, to DOBIS/E acquisition system because we found that our DOBIS/E acquision system is able to use as we do ordenng and receiving in traditional process. DOBIS/E system and three added subsystems is closely related, and allow acquisition librarians to work more efficiently than before.

• Journal of Arbitration Studies
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• v.16 no.1
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• pp.121-151
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• 2006

As globalisation extends its effect and particularly following China's accession to the World Trade Organization(WTO) in 2001, ever greater numbers of international transactions will feature a Chinese party. China has certainly made efforts in recent years to rectify law problem. While conducting business in China, foreign companies occasionally find themselves embroiled in disputes with Chinese individuals and companies. As foreign businesses invest in the extraordinary market opportunities in China, international arbitration has also become the preferred method for handling disputes with Chinese partners or with other foreign corporation over operations in China. The new Arbitration Rules of the International Economic and Trade Arbitration Commission(CIETAC) came into force on 1 May 2005. The new rules represent a major overhaul of CIETAC arbitration procedures and are sure to enhance CIETAC's position as a leading player in the resolution of China-foreign business disputes. The changes are significant for all companies doing business in China. So, this article investigated some amendments on the basis of 2000 Rules.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.106-108
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• 2007

The Symptoms of witches' broom disease caused by phytoplasma including general stunting and yellowing, were observed in leafy lespedeza (Lespedeza cyrtobotrya M.) on Doam-myeon, Pyeongchang-gun, in 2006. Based on the sequence analysis of PCR-amplified 16S ribosomal DNA and 16S-23S spacer region DNA products using universal phytoplasma primers, the phytoplasma associated with leafy lespedeza witches' broom (LLWB) disease was identified as a member of Candidatus Pytoplasma trifolii. It was most closely related to alsike clover proliferation phytoplasma (99.8% similarity, accession no. AY390261), Candidatus Pytoplasma trifolii strain. RFLP patterns generated with AluI, HpaII clearly differentiated LLWB phytoplasma from the referenced phytoplasma strains, water dropwort witches' broom, mulberry dwarf, glehni aster yellow dwarf and jujube witches' broom. This paper is the first report on Candidatus Phytoplasma trifolii in leafy lespedeza identified at a molecular level.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.325-328
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• 2003

폴드와 타 동유럽국가들이 내년에 유럽연합(EU)에 가입할 예정이다. EU에 가입한 후 폴란드는 도로분야의 투자에 박차를 가한 것이며 2005년까지 도로망을 보수할 것이다. 현재 폴란드 정부가 도로의 모든 건설투자 비용을 충당할 수 없으므로 민${\cdot}$관 협력에 기초하여 프로젝트 자본조달에 관한 새로운 방법을 발전시키고자 한다. 고도로 개발된 도로 교통망 없이는 국가 경제측면에서 경쟁력을 제고할 수 없으므로 EU 전체의 도로 교통망을 도입 하고자 한다. 도로 교통망의 성공적인 확대는 폴란드와 같이 아직 EU에 소속되지 않은 동유럽 국가의 교통 기반시설의 기초가 될 것이며 이것은 전채유럽의 이익을 가져 올 것이다.

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1115-1120
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• 2008

A novel cathepsin B inhibitor-producing bacterium was isolated from marine sediments and identified based on its 16S rDNA sequence as Pseudomonas sp. strain PB01 (Accession No. EU126129). The growth and enzyme inhibitor production were investigated under various culture conditions. A mixture of organic nitrogen source was required for the optimal production, whereas both glucose and maltose proved to be the effective carbon sources for cathepsin B inhibitor production. Other optimal culture conditions included temperature range between 25 and $28^{\circ}C$, initial medium pH of 6.6, and shaking speed of 200 rpm. Under these optimal conditions, the maximum inhibitory activity from culture broth was approximately 50% after 30 h of cultivation. Additionally, kinetic study revealed that inhibitor production paralleled with cell growth, which suggested that the inhibitor may be a primary metabolite of that bacterium.

• Journal of fish pathology
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• v.16 no.3
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• pp.215-217
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• 2003

This study examined whether the connexin (Cx) is an essential protein during oocyte maturation in the ovary of the goldfish (Carassius auratus). In mature female goldfish ovaries, at late vitellogenic stage, human insulin-like growth factor-I (IGF-I; 20 M) and human chorionic gonadotropin(hCG; 20 IU/㎖) were injected. Twelve hr after the injection, mature female goldfish ovaries were removed and stored at -80C until analysis by RT-PCR. From the goldfish Cx43 cDNA sequence (GenBank accession number AB078505), two degenerate primers were designated. In vivo, 12 hr after the treatment with hCG, goldfish Cx43 mRNA expression level was increased, while the levels of IGF-I was not changed. Goldfish Cx43 mRNA expressed after, but not before the hCG treatment. These results suggest that Cx43 mRNA was judged to be a gene, which was transcribed during oocyte maturation induced by hCG.