• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.1
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• pp.119-129
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• 2018

Companies' efforts to secure and sustain a competitive advantage in the market may also appear in the form of new product development. This has increased the importance of leveraging knowledge from other companies in a global competition that is accelerating with an accelerating product life cycle and the development of transportation and communications. In order to create valuable knowledge through the exchange and combination of knowledge, the absorptive capacity of the recipient of the knowledge should be considered as important, but also the knowledge protection tendency, which is characteristic of the knowledge transferor, should be considered. However, studies that considered these factors at the same time were very limited. This study examines the effect of suppliers 'absorptive capacity and buyer' s knowledge protection on new product development performance during the joint development of new products. In addition, we investigated the interaction effect between supplier's absorption capacity and buyer's knowledge protection tendency. For this purpose, data were collected for automobile industry, electronics industry and defense industry, where joint development of new products occurred frequently, and statistical verification was performed by regression analysis. The results showed that the absorptive capacity of the supplier was positively correlated with the performance of the new product development, and the buyer 's knowledge protection tendency had a negative relationship with the new product development performance. In addition, the absorptive capacity of the supplier and the buyer 's knowledge protection tendency have an interaction effect on the new product development result. These results suggest that suppliers' absorption capacity is an important factor in the joint development of new products, but suppliers with superior absorption capacity may have a negative impact on new product development performance if buyers have a negative attitude toward knowledge transfer.

• Journal of Digital Contents Society
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• v.15 no.2
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• pp.301-308
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• 2014

The mass storage of data and the importance of storage capacity lately has emerged. In this paper, we suggest the new method which estimate life cycle from acceleration life test about 2.5 inch hard disk driver as mass storage, which minimize the difference between real market annual failure rate and estimated failure rate. we obtain market transfer capacity per hours from returned 2.5inch hard disk drives about 309 numbers and accelerating transfer capacity per hours from acceleration life test. we calculate acceleration factor about duty cycle and estimate annual failure rate from failure rate and acceleration factors: temperature acceleration factor, duty cycle acceleration factor, the transfer capacity per hour represent the 93.4 % of user is 5.8 GB/hour, the transfer capacity per hour from acceleration life test is 81.4 GB/hour. the acceleration factor of duty cycle is 14.1. annual failure rate is stabilized at market one year after, annual failure rate is between 0.3 and 0.378. estimated annual failure rate is 0.4, so it is matched at 75~94 % between estimate annual failure rate and market annual failure rate. This study influence the estimate method of annual failure rate by the result of acceleration life cycle of manufacture.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.409-414
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• 2006

Molecular chaperones and folding enzymes in the endoplasmic reticulum (ER) associate with the newly synthesized proteins to prevent their aggregation and help them fold and assemble correctly. Chaperone function of BiP, which is a Hsp70 homologue in ER, is controlled by the N-terminal ATPase domain. The ATPase activity of the ATPase domain is affected by regulatory factors. BAP was identified as a nucleotide exchange factor of BiP (Grp78), which exchanges ADP with ATP in the ATPase domain of BiP This study presents whether BAP can influence folding of a protein, immunoglobulin heavy chain that is bound to BiP tightly. We first examined which nucleotide of ADP and ATP affects on BAP binding to BiP The data showed that endogenous BAP of HEK293 cells prefers ADP for binding to BiP in vitro, suggesting that BAP first releases ADP from the ATPase domain in order to exchange with ATP. Immunoglobulin heavy chain, an unfolded protein substrate, was released from BiP in the presence of BAP but not in the presence of ERdj3, which is another regulatory factor for BiP accelerating the rate of ATP hydrolysis of BiP The ADP-releasing function of BAP was, therefore, believed to be responsible for immunoglobulin heavy chain release from BiP. Grp170, another Hsp70 homologue in ER, did not co-precipited with BAP from $[^{35}S]$-metabolic labeled HEK293 lysate containing both overexpressed Grp170 and BAP. These data suggested that BAP has no specificity to Grp170 although the ATPase domains of Grp170 and BiP are homologous each other.

• Natural Product Sciences
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• v.11 no.3
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• pp.139-144
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• 2005

Bioflavone quercetin is believed to play an important role preventing bone loss by affecting osteoclastogenesis and regulating many systemic and local factors including hormones and cytokines. This study examined how quercetin acts on tumor necrosis factor-alpha ($TNF-{\alpha}$)-mediated apoptosis in MC3T3-E1 osteoblastic cells. Apoptosis assays revealed the dose-dependent acceleration of quercetin on $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 cells, which was demonstrated by the increased number of positively stained cells in the trypan blue staining and TUNEL assay, and the migration of many cells to the $sub-G_0/G_1$ phase in flow cytometric analysis. In particular, quercetin treatment alone increased the expression of p53 and p21 proteins in the cells. Consequently, this study showed that quercetin accelerates the $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 osteoblastic cells.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.271-277
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• 2006

The interaction effects of ozone $(O_3)$ and anthracnose (Colletotrichum acutatum) disease were examined in green fruits and seedlings of pepper (Capsicum annuum). Pre-treatment with $(O_3)$ as a factor causing predisposition to the disease prior to infection was investigated in green fruits and stems using an $(O_3)$ concentration of 150 nL/L, which is easily reached in summer in Korea. $(O_3)$ treatment increased antioxidative responses in pepper foliar tissues, and defense against anthracnose was examined in fruits and stems. Anthracnose severity on stems of the $O_3-treated$, ozone-sensitive 'Dabotop' cultivar was always lower than that on untreated plants, but the difference was not always significant (p=0.147). Significantly lower anthracnose severity was found on $O_3-treated$ green 'Dabotop' fruits as compared to untreated green fruits in three of eight replicate experiments. In contrast, hypersensitive responses in 03treated seedlings were significantly accelerated compared to those in untreated seedlings by about 7.8 h (p<0.001). This confirmed previous evidence of increased transcription of plant defense genes with $(O_3)$ treatment. $(O_3)$ treatment significantly decreased chlorophyll concentrations in the leaves in four replicate experiments (p<0.01). $(O_3)$ increased hypersensitive responses in the leaves of pepper seedlings, but this increase did not contribute to the control of anthracnose severity on fruits. Antioxidant reactions to $(O_3)$ were limited to chlorosis and changes in hypersensitive responses in leaves.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1407-1415
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• 2016

Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. Two types of cells, immature sertoli cell and type A spermatogonia were observed in buffalo testes in this stage. Germ cell marker genes, OCT3/4 (Pou5f1), THY-1, c-kit, PGP9.5 (UCHL-1) and Dolichos biflorus agglutinin, were determined to be expressed both in mRNA and protein level by reverse transcription polymerase chain reaction and immunostaining in buffalo testes and buffalo spermatogonial stem-like cells, respectively. In the following, when the isolated buffalo buffalo spermatogonial stem-like cells were cultured in the medium supplemented 2.5% fetal bovine serum and 40 ng/mL glial cell-derived neurotrophic factor medium, SSCs proliferation efficiency and colony number were significantly improved than those of other groups (p<0.05). These findings may help in isolation and establishing long term in vitro culture system for buffalo spermatogonial stem-like cells, and accelerating the generation of genetic modified buffaloes.

• Journal of the Korean GEO-environmental Society
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• v.2 no.3
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• pp.57-69
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• 2001

Sand pile is widely used as a ground improvement method. Although the primary purpose of constructing sand pile is accelerating consolidation, composite ground effect also can be gained by constructing sand pile. This study was accomplished to understand composite ground effect on the ground improved by sand piles which were applied as vertical drainage material when area replacement ratio was small relatively. For determining bearing capacities of origin ground and sand piles and analysing interaction between embankment and origin ground, bearing tests and earth pressure monitoring are performed. From the results, it turned out that the contribution of sand pile as a load bearing mechanism is not substantial. However, the bearing capacity of sand pile was increased to sixty percentages when compared with origin ground. The increasement of bearing capacity could be caused the change of consolidation characteristics during the process of consolidation by overburden load. Therefore, the composite ground effects depending on stiffness increasement of sand pile would be estimated as a factor decreasing consolidation settlement.

• Korean Institute of Interior Design Journal
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• v.14 no.5 s.52
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• pp.106-113
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• 2005

New images percolate through human consciousness by the media such as movies, TV programs, and brilliant advertisements. These images reproduce new ' things ' throughout the ' semantic processes ' by those who experience and recognize them. Alvin Toffler describes it as the ' information bomb ' and ' image fragments ' in his talk about the new paradigm of information-oriented era. The increasing number of images and their accelerating rate of appearance imply that images become more momentary, and are evidence that they are transforming entire human life and consciousness. Such awareness means a lot to a designer. Especially, the subject that how modern space-dominating images are related to the structure and materials constituting the space and communicate with human mind will be an important factor in establishing the human-space relationship in the future. Furthermore, the present age overspread with various medium is not the only one privileged of the images that exist within space. They are the results of continuous expansion of existing images, and also process of evolution of space powered by the fusion of images and digital media. Imagified space is a boundary layer of Cyberspace, and the space itself becomes an interface by human recognition and participation. Now, the functional classification of spaces such as ' office, ' ' cafe, ' and ' school ' is meaningless. Whatever it may be, the function of a space is defined by the information it interfaces, and therefore it becomes an interface to information through a large number of images. Based on this idea, we will observe the imagificaiton of space in the form of discussion, and from that, try to understand the phenomenon through the real world examples.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.437-443
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• 2003

Bioremediation technologies were applied to experimental microcosms, simulating an oil spill in a lower intertidal area. Three treatments (oil only, oil plus nutrients, and oil plus nutrients and microbial inocula) were applied, and each microcosm was repeatedly filled and eluted with seawater every 12 h to simulate tidal cycles. To minimize washing-out of the inoculum by the tidal cycles, microbial cells were primarily immobilized on diatomaceous earth before they were applied to the oiled sand. Oil degradation was monitored by gravimetric measurements, thin layer chromatography/flame ionization detector (TLC/FID) analysis, and gas chromatography (GC) analysis, and the loss of oil content was normalized to sand mass or nor-hopane. When the data were normalized to sand mass, no consistent differences were detected between nutrient-amended and nutrient/inoculum-amended microcosms, although both differed from the oil-only microcosm in respect of oil removal rate by a factor of 4 to 14. However, the data relative to nor-hopane showed a significant treatment difference between the nutrient-amended and nutrient/inoculum-treated microcosms, especially in the early phase of the treatment. The accelerating effect of inoculum treatment has hardly been reported in studies of oil bioremediation in the Tower intertidal area. The inoculum immobilized on diatomaceous earth seemed to be a very effective formulation for retaining microbial cells in association with the sand. Results of this study also suggest that interpretation of the effectiveness of bioremediation could be dependent on the selection of monitoring methods, and consequently the application of various analytical methods in combination could be a solution to overcome the limitations of oil bioremediation monitoring.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.1
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• pp.209-214
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• 2009

Due to the high level of information supportive function of Internet, the management environment change is accelerating. The Patients are able to have the chances of Hospital- and Doctor-shopping. So the Customer Relationship Management skill or Patient -oriented management is becoming one of the important management method of Hospital. To adapt these trend, many Hospital invest the money and efforts for the implementation of Hospital CRM system. But the Performance of HCRM is not satisfactory for the investor from many reasons. In this paper we try to find out which kinds of factors have the Influences on the Implementation of HCRM, and how is the relation between these factors. We use the Structural Equation Model to clarify these relationships. The result of this paper will contribute in the decision making of the implementation of HCRM in the field, and in providing the knowledge base.