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Accelerating Effects of Quercetin on the $TNF-{\alpha}-Induced$ Apoptosis in MC3T3-E1 Osteoblastic Cells  

Choi, Yong-Sung (Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, School of Dentistry)
Chung, Song-Woo (Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, School of Dentistry)
Jeon, Young-Mi (Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, School of Dentistry)
Kim, Jong-Ghee (Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, School of Dentistry)
Lee, Jeong-Chae (Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, School of Dentistry,Research Center of Bioactive Materials, Chonbuk National University)
Publication Information
Natural Product Sciences / v.11, no.3, 2005 , pp. 139-144 More about this Journal
Abstract
Bioflavone quercetin is believed to play an important role preventing bone loss by affecting osteoclastogenesis and regulating many systemic and local factors including hormones and cytokines. This study examined how quercetin acts on tumor necrosis factor-alpha ($TNF-{\alpha}$)-mediated apoptosis in MC3T3-E1 osteoblastic cells. Apoptosis assays revealed the dose-dependent acceleration of quercetin on $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 cells, which was demonstrated by the increased number of positively stained cells in the trypan blue staining and TUNEL assay, and the migration of many cells to the $sub-G_0/G_1$ phase in flow cytometric analysis. In particular, quercetin treatment alone increased the expression of p53 and p21 proteins in the cells. Consequently, this study showed that quercetin accelerates the $TNF-{\alpha}-induced$ apoptosis in MC3T3-E1 osteoblastic cells.
Keywords
Quercetin; $TNF-{\alpha}$; MC3T3-E1 osteoblastic cells; apoptosis;
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