• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.634-643
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• 2024

Juglans mandshurica Maxim. walnut (JMW) is well-known for the treatment of dermatosis, cancer, gastritis, diarrhea, and leukorrhea in Korea. However, the molecular mechanism underlying its antiobesity activity remains unknown. In the current study, we aimed to determine whether JMW can influence adipogenesis in 3T3-L1 preadipocytes and high-fat diet rats and determine the antioxidant activity. The 20% ethanol extract of JMW (JMWE) had a total polyphenol content of 133.33 ± 2.60 mg GAE/g. Considering the antioxidant capacity, the ABTS and DPPH values of 200 ㎍/ml of JMWE were 95.69 ± 0.94 and 79.38 ± 1.55%, respectively. To assess the anti-obesity activity of JMWE, we analyzed the cell viability, fat accumulation, and adipogenesis-related factors, including CCAAT-enhancer-binding protein alpha (C/EBPα), sterol regulatory element-binding protein-1c (SREBP1c), peroxisome proliferator-activated receptor-gamma (PPARγ), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC). We found that total lipid accumulation and triglyceride levels were reduced, and the fat accumulation rate decreased in a dose-dependent manner. Furthermore, JMWE suppressed adipogenesis-related factors C/EBPα, PPARγ, and SREBP1c, as well as FAS and ACC, both related to lipogenesis. Moreover, animal experiments revealed that JMWE could be employed to prevent and treat obesity-related diseases. Hence, JMWE could be developed as a healthy functional food and further explored as an anti-obesity drug.

• Journal of Animal Science and Technology
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• 제54권5호
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• pp.369-373
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• 2012

AMP-activated protein kinase (AMPK)는 체내 에너지 수준을 감지하고 당과 지방의 대사를 조절하는 인자로 밝혀졌다. 이러한 에너지 센서로서 AMPK의 역할은 심혈관계 및 비만과 당뇨 등의 대사성 질환에 밀접한 관계가 있다. 영양적 관점에서 AMPK는 지방산의 합성 및 분해를 통해 간에서 지방대사 조절에 중요한 역할을 한다. 특히 근육의 경우 glucose 흡수를 관장하며, 근육세포 내 glucose의 유입을 증가 시킨다. 하지만, AMPK의 활성이 대사기질의 흡수와 이용에 미치는 영향에 대한 정확한 기전은 아직까지 불분명하다. 또한 영양적 수준 차이에 따른 AMPK의 활성이 단백질 합성에 미치는 영향은 아직 보고된 바 없다. 따라서 본 연구의 목적은 C2C12 myotube에서 AMPK 활성물로 알려진 5-aminoimidazole-4-carbozamide-1-${\beta}$-D-ribonucleoside (AICAR)가 glucose 농도차이에 따른 AMPK 활성과 단백질함량에 미치는 영향을 알아보기 위함이다. 배양된 C2C12 근육세포에서 AICAR는 glucose의 함량에 관계없이 AMPK를 활성화 시켰다. 단백질 농도는 낮은 수준 (LG)에 비해 높은 수준의 glucose (HG)가 처리된 myotube에서 증가되었고, AICAR에 의해 그 효과는 더욱 증대 되었다. C2C12 myotube에서 HG 처리는 AMPK와 acetyl CoA carboxylase (ACC)의 인산화에 영향을 주지 않았지만, LG의 처리로 인해 AMPK와 ACC의 인산화가 증가되었다 (p<0.05). 또한, AICAR (2mM)의 처리는 glucose의 수준과는 관계없이 AMPK와 ACC의 인산화를 증가시켰다. 총 단백질 수준은 HG 처리에 의해 증가 되었고, 이는 AICAR의 처리에 의해서 더 높게 증가되었다. Proteasome 활성은 HG 처리구에서 LG에 비해 7.5% 낮게 나타났고, AICAR 처리는 proteasome 활성을 LG와 HG 처리구에서 각각 63%와 54% 감소 시켰다. Glucose의 수준은 mTOR의 인산화 수준에 영향을 주지 않았다. 하지만, AICAR는 LG 처리구에서 만 mTOR의 인산화 수준을 유의적으로 증가시켰고, HG 처리구에는 mTOR에 영향을 주지 않았다. 따라서, glucose 처리는 단백질을 proteasome으로부터 보호 하거나, glucose가 AMPK의 단백질 합성 저해 기능을 방해하여 세포내 단백질 합성을 중가 시키는 것으로 사료 된다. 결론적으로, 영양적 수준에 의해 AMPK 활성 및 단백질 합성과 분해가 조절된다는 것을 의미한다.

• International Journal of Control, Automation, and Systems
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• 제3권2호
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• pp.173-182
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• 2005

In this paper, a target tracking algorithm for tracking maneuvering vehicles is presented. The overall algorithm belongs to the category of an interacting multiple-model (IMM) algorithm used to detect multiple targets using fused information from multiple sensors. First, two kinematic models are derived: a constant velocity model for linear motions, and a constant-speed turn model for curvilinear motions. Fpr the constant-speed turn model, a nonlinear information filter is used in place of the extended Kalman filter. Being equivalent to the Kalman filter (KF) algebraically, the information filter is extended to N-sensor distributed dynamic systems. The model-matched filter used in multi-sensor environments takes the form of a federated nonlinear information filter. In multi-sensor environments, the information-based filter is easier to decentralize, initialize, and fuse than a KF-based filter. In this paper, the structural features and information sharing principle of the federated information filter are discussed. The performance of the suggested algorithm using a Monte Carlo simulation under the two patterns is evaluated.

• Advances in concrete construction
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• 제2권1호
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• pp.1-11
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• 2014

This paper demonstrates an experimental study to evaluate the effects of environmental exposures on the bond between ribbed Glass Fiber Reinforced Polymer (GFRP) reinforcing bars and concrete. The equation recommended by ACI 440-1R-06, for the bond stress,was evaluated in this study. A total of 16 pullout samples, 12with GFRP bars and 4with steel bars, were exposed to two different harsh environments for different periods of time. The exposed harsh environments included direct sun exposure and cyclic splash zone sea water. The variation in the shear (bond) strengths before and after exposure was considered as a measure of the durability of the bond between GFRP bars and concrete.Experimental results showed there is no significant difference of the bond strength between 60 and 90 days of exposures.It also showed that the empirical equation of the bond stress calculated by ACI 440-IR-06 is very conservative.

• Advances in concrete construction
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• 제2권1호
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• pp.39-56
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• 2014

Corrosion in RCC structures is one of the most important factors that affects the structure's durability and subsequently causes reduction of serviceability. The most severe cause of this corrosion is chloride attack. Hence, to prevent this to happen proper understanding of the chloride penetration into concrete structures is necessary. In this study, first the mechanism of this chloride attack is understood and various parameters affecting the process are identified. Then an FEM modelling is carried out for the chloride diffusion process. The effects of fly ash and slag on the diffusion coefficient and chloride penetration depth in various mixes of concretes are also analyzed through integrating Virtual RCPT Lab and FEM.

• Advances in concrete construction
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• 제2권1호
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• pp.57-75
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• 2014

This paper presents an analysis of the results of an experimental program on the performance of a novel configuration of a hybrid FRP-concrete beam. The beam section consists of a GFRP pultruded profile, a CFRP laminate, and a concrete block all wrapped up using filament winding. It was found that the thickness of the concrete block and the confinement by the filament-wound wrapping had a profound effect on the energy dissipation behaviour of the beam. Using a shear punching model, and comparing the predicted results with the experimental ones, it was found that beyond a given value of the concrete block thickness, the deformational behaviour of the beam shifts from brittle to ductile. It was also found that the filament-wound wrap had many benefits such as providing a composite action between the concrete block and the GFRP box, improving the stiffness of the beam, and most importantly, enhancing the load carrying ability through induced confinement of the concrete.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.773-777
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• 2008

Phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of Chinese cabbage and screened on the basis of their solubilization of inorganic tricalcium phosphate in liquid cultures. Ten strains that had higher solubilization potential were selected, and they also produced indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and siderophores. The strains were identified to be members of Pseudomonas, by 16S rDNA sequence analysis. Seed bacterization with PSB strains increased the root elongation and biomass of Chinese cabbage in seedling culture, although they had no effect on phosphorus uptake of plants. The plant growth promotion by PSB in this study could be due to the production of phytohormones or mechanisms other than phosphate solubilization, since they had no effect on P nutrition.

• 제어로봇시스템학회논문지
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• 제17권11호
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• pp.1145-1153
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• 2011

EMB (Electro-Mechanical Brake) systems can provide improved braking and stability functions such as ABS, EBD, TCS, ESC, BA, ACC, etc. For the implementation of the EMB systems, reliable and robust fault detection algorithm is required. In this study, a model-based fault detection algorithm is designed based on the analytical redundancy method in order to monitor current and force sensor faults in EMB systems. A state-space model for the EMB is derived including faulty signals. The fault diagnosis algorithm is constructed using the analytical redundancy method. Observer is designed for the EMB and the fault detectability condition is examined based on the residual analysis. The performance of the proposed model-based fault detection algorithm is verified in simulations. The effectiveness of the proposed algorithm is demonstrated in various faulty cases.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.1130-1133
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• 2003

The goal of this research is developing advanced lane detecting algorithm for unmanned vehicle. Previous lane detecting method to bring on error become of the lane loss and noise. Therefore, new algorithm developed to get exact information of lane. This algorithm can be used to AGV(Autonomous Guide Vehicle) and LSWS(Lane Departure Warning System), ACC(Adapted Cruise Control). We used 1/10 scale RC car to embody developed algorithm. A CCD camera is installed on top of vehicle. Images are transmitted to a main computer though wireless video transmitter. A main computer finds information of lane in road image. And it calculates control value of vehicle and transmit these to vehicle. This algorithm can detect in input image marked by 256 gray levels to get exact information of lane. To find the driving direction of vehicle, it search line equation by curve fitting of detected pixel. Finally, author used median filtering method to removal of noise and used characteristic part of road image for advanced of processing time.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.101-106
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• 2003

In the area of tracking control, it is important to design not only the controllers but also the trajectories to which a system has to follow. $5^{th}$ order polynomial is often used with constraints of initial and final states. Smooth ending with possible minimum time is important for many systems because of vibration or jerky motions. Examples are increased with development of technology in smaller, more accurate systems. On the base of a polynomial like trajectory generation method from a paper in ACC2002 and RIC(Robust Internal-loop Compensator) control scheme of Robotics and Bio-mechanics lab. of POSTECH, generalized and expanded polynomial like trajectory generation method is showed.