• The Plant Pathology Journal
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• 제30권3호
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• pp.323-329
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• 2014

Two cultivars Buram-3-ho (susceptible) and CR-Hagwang (moderate resistant) of kimchi cabbage seedlings showed differential defense responses to anthracnose (Colletotrichum higginsianum), black spot (Alternaria brassicicola) and black rot (Xanthomonas campestris pv. campestris, Xcc) diseases in our previous study. Defense-related hormones salicylic acid (SA), jasmonic acid (JA) and ethylene led to different transcriptional regulation of pathogenesis-related (PR) gene expression in both cultivars. In this study, exogenous application of SA suppressed basal defenses to C. higginsianum in the 1st leaves of the susceptible cultivar and cultivar resistance of the 2nd leaves of the resistant cultivar. SA also enhanced susceptibility of the susceptible cultivar to A. brassicicola. By contrast, SA elevated disease resistance to Xcc in the resistant cultivar, but not in the susceptible cultivar. Methyl jasmonate (MJ) treatment did not affect the disease resistance to C. higginsianum and Xcc in either cultivar, but it compromised the disease resistance to A. brassicicola in the resistant cultivar. Treatment with 1-aminocyclopropane-1-carboxylic acid (ACC) ethylene precursor did not change resistance of the either cultivar to C. higginsianum and Xcc. Effect of ACC pretreatment on the resistance to A. brassicicola was not distinguished between susceptible and resistant cultivars, because cultivar resistance of the resistant cultivar was lost by prolonged moist dark conditions. Taken together, exogenously applied SA, JA and ethylene altered defense signaling crosstalk to three diseases of anthracnose, black spot and black rot in a cultivar-dependent manner.

• Atmosphere
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• 제28권3호
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• pp.233-245
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• 2018

This study explores the Subseasonal-to-Seasonal (S2S) prediction skills of the Northern Hemisphere mid-latitude geopotential height in the Global Seasonal forecasting model version 5 (GloSea5) hindcast experiment. The prediction skills are quantitatively verified for the period of 1991~2010 by computing the Anomaly Correlation Coefficient (ACC) and Mean Square Skill Score (MSSS). GloSea5 model shows a higher prediction skill in winter than in summer at most levels regardless of verification methods. Quantitatively, the prediction limit diagnosed with ACC skill of 500 hPa geopotential height, averaged over $30^{\circ}N{\sim}90^{\circ}N$, is 11.0 days in winter, but only 9.1 days in summer. These prediction limits are primarily set by the planetary-scale eddy phase errors. The stratospheric prediction skills are typically higher than the tropospheric skills except in the summer upper-stratosphere where prediction skills are substantially lower than upper-troposphere. The lack of the summer upper-stratospheric prediction skill is caused by zonal mean error, perhaps strongly related to model mean bias in the stratosphere.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.357-364
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• 2017

In this study, we aimed to generate a series of versatile tagging plasmids that can be used in diverse molecular biological studies of the fungal pathogen Cryptococcus neoformans. We constructed 12 plasmids that can be used to tag a protein of interest with a GFP, mCherry, $4{\times}FLAG$, or $6{\times}HA$, along with nourseothricin-, neomycin-, or hygromycin-resistant selection markers. Using this tagging plasmid set, we explored the adenylyl cyclase complex (ACC), consisting of adenylyl cyclase (Cac1) and its associated protein Aca1, in the cAMP-signaling pathway, which is critical for the pathogenicity of C. neoformans. We found that Cac1-mCherry and Aca1-GFP were mainly colocalized as punctate forms in the cell membrane and non-nuclear cellular organelles. We also demonstrated that Cac1 and Aca1 interacted in vivo by co-immunoprecipitation, using $Cac1-6{\times}HA$ and $Aca1-4{\times}FLAG$ tagging strains. Bimolecular fluorescence complementation further confirmed the in vivo interaction of Cac1 and Aca1 in live cells. Finally, protein pull-down experiments using $aca1{\Delta}$::ACA1-GFP and $aca1{\Delta}$::ACA1-GFP $cac1{\Delta}$ strains and comparative mass spectrometry analysis identified Cac1 and a number of other novel ACC-interacting proteins. Thus, this versatile tagging plasmid system will facilitate diverse mechanistic studies in C. neoformans and further our understanding of its biology.

• The Korea Journal of Herbology
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• 제35권5호
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• pp.23-31
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• 2020

Objectives : This study was conducted to evaluate the anti-hyperlipidemia effect of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix(SAB). Methods : FL83B cells were mouse liver hepatocytes, and we used this cell line. FL83B cells were treated with 0.5 mM oleic acid(OA) for 24 h, SAB extract was treated. After OA treatment, intracellular triglyceride (TG) and free fatty acid contents were measured with AdiopoRed™ assay and Free Fatty Acid Quantitation assay kit, respectively. Further, we evaluated several lipogenesis and metabolic markers such as sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), 3-hydroxy3-methyl-glutaryl CoA reductase (HMGCR), hormone-sensitive lipase (HSL), carnitine palmitoyltransferase (CPT-1), peroxisome proliferator activated receptor alpha (PPARα), and cluster of differentiation (CD36) using RT-PCR and Western-blot analysis. Results : OA markedly increased intracellular TG and free fatty acid, which plays a key role in reducing hepatic lipid accumulation, in FL83B cells. These increases were alleviated by SAB extract. The mRNA and protein expression of Fatty acid(FA) oxidation factors (CPT-1, PPARα), lipolysis factor(HSL), FA transporter(CD36), cholesterol synthesis factors (HMGCoA) and Lipodenesis (SREBP-1c, FAS, and ACC-1) were significantly increased by treatment of SAB extract in the OA-induced fatty liver cell model. Conclusions : In summary, the treat of SAB extract showed a significant reduction of the influx of fatty acids into hepatocytes, promoted the oxidation of fatty acids, and regulated fat synthesis-related factors, thereby regulating the accumulation of TG and free fatty acids.

• Journal of Oral Medicine and Pain
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• 제41권1호
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• pp.30-33
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• 2016

Malignant tumors located in the head and neck areas intermittently show symptoms similar to a temporomandibular disorder (TMD). In our case, a patient who first visited us complaining of TMD-like symptoms, such as trismus and temporomandibular joint (TMJ) pain on the left side, was identified as a sufferer of adenoid cystic carcinoma (ACC) that arose from the left maxillary sinus. The patient may have a TMD symptom, but we are skeptical because the patient also complained of a spontaneously developed numbness on the same side of his upper lip. We observed the panoramic radiograph closely and found the blurred maxillary sinus inferior wall. Dental cone-beam computed tomography confirmed the tentative diagnosis of malignancy on the maxillary sinus. After he had been referred to the Department of Otolaryngology, the diagnosis of the ACC was confirmed. Adequate diagnosis is vital for a quick treatment of the malignancy. There are some keys for differential diagnosis of TMD-like symptoms.

• Earthquakes and Structures
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• 제5권1호
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• pp.49-65
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• 2013

This article presents the statistical characteristics of elastic floor acceleration spectra that represent the peak response demand of non-structural components attached to a nonlinear supporting frame. For this purpose, a set of stiff and flexible general moment resisting frames with periods of 0.3-3.6 sec. are analyzed using forty-nine near-field strong ground motion records. Peak accelerations are derived for each single degree of freedom non-structural component, supported by the above mentioned frames, through a direct-integration time-history analysis. These accelerations are obtained by Floor Acceleration Response Spectrum (FARS) method. They are statistically analyzed in the next step to achieve a better understanding of their height-wise distributions. The factors that affect FARS values are found in the relevant state of the art. Here, they are summarized to evaluate the amplification and/or reduction of FARS values especially when the supporting structures undergo inelastic behavior. The properties of FARS values are studied in three regions: long-period, fundamental-period and short-period. Maximum elastic acceleration response of non-structural component, mounted on inelastic frames, depends on the following factors: inelasticity intensity and modal periods of supporting structure; natural period, damping ratio and location of non-structural component. The FARS values, corresponded to the modal periods of supporting structure, are strongly reduced beyond elastic domain. However, they could be amplified in the transferring period domain between the mentioned modal periods. In the next step, the amplification and/or reduction of FARS values, caused by inelastic behavior of supporting structure, are calculated. A parameter called the response acceleration reduction factor ($R_{acc}$), has been previously used for far-field earthquakes. The feasibility of extending this parameter for near-field motions is focused here, suggested repeatedly in the relevant sources. The nonlinearity of supporting structure is included in ($R_{acc}$) for better estimation of maximum non-structural component absolute acceleration demand, which is ordinarily neglected in the seismic design provisions.

• Nuclear Engineering and Technology
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• 제52권5호
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• pp.937-946
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• 2020

The aim in the present work is to simulate accident scenarios of AP1000 during the small-break loss-of-coolant accident (SBLOCA) and investigate the performance and behavior of automatic depressurization system (ADS) during accidents by using MIDAC (The Module In-vessel Degradation severe accident Analysis Code). Four types of accidents with different hypothetical conditions were analyzed in this study. The impact on the thermal-hydraulic of the reactor coolant system (RCS), the passive core cooling system and core degradation was researched by comparing these types. The results show that the RCS depressurization becomes faster, the core makeup tanks (CMT) and accumulators (ACC) are activated earlier and the effect of gravity water injection is more obvious along with more ADS valves open. The open of the only ADS1-3 can't stop the core degradation on the basis of the first type of the accident. The open of ADS1-3 has a great impact on the injection time of ACC and CMT. The core can remain intact for a long time and the core degradation can be prevent by the open of ADS-4. The all results are significant and meaningful to understand the performance and behavior of the ADS during the typical SBLOCA.

• Journal of Veterinary Science
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• 제22권4호
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• pp.55.1-55.17
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• 2021

Background: Naringenin and its glycoside naringin are well known citrus flavonoids with several therapeutic benefits. Although the anti-adipogenic effects of naringenin and naringin have been reported previously, the detailed mechanism underlying their anti-adipogenesis effects is poorly understood. Objectives: This study examined the anti-adipogenic effects of naringenin and naringin by determining differential gene expression patterns in these flavonoids-treated 3T3-L1 adipocytes. Methods: Lipid accumulation and triglyceride (TG) content were determined by Oil red O staining and TG assay. Glucose uptake was measured using a 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose fluorescent d-glucose analog. The phosphorylation levels of AMP-activated protein kinase (AMPK) and acetyl Co-A carboxylase (ACC) were observed via Western blot analysis. Differential gene expressions in 3T3-L1 adipocytes were evaluated via RNA sequencing analysis. Results: Naringenin and naringin inhibited both lipid accumulation and TG content, increased phosphorylation levels of both AMPK and ACC and decreased the expression level of 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) in 3T3-L1 adipocytes. RNA sequencing analysis revealed that 32 up-regulated (> 2-fold) and 17 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Scd1, Mogat1, Dgat, Lipin1, Cpt1a, and Lepr, were normalized to the control level in naringenin-treated adipocytes. In addition, 25 up-regulated (> 2-fold) and 25 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Fabp5, Scd1, Srebf1, Hmgcs1, Cpt1c, Lepr, and Lrp1, were normalized to the control level by naringin. Conclusions: The results indicate that naringenin and naringin have anti-adipogenic potentials that are achieved by normalizing the expression levels of lipid metabolism-related genes that were perturbed in differentiated 3T3-L1 cells.

• Molecules and Cells
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• 제41권12호
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• pp.1033-1044
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• 2018

As sessile organisms, plants have evolved to adjust their growth and development to environmental changes. It has been well documented that the crosstalk between different plant hormones plays important roles in the coordination of growth and development of the plant. Here, we describe a novel recessive mutant, mildly insensitive to ethylene (mine), which displayed insensitivity to the ethylene precursor, ACC (1-aminocyclopropane-1-carboxylic acid), in the root under the dark-grown conditions. By contrast, mine roots exhibited a normal growth response to exogenous IAA (indole-3-acetic acid). Thus, it appears that the growth responses of mine to ACC and IAA resemble those of weak ethylene insensitive (wei) mutants. To understand the molecular events underlying the crosstalk between ethylene and auxin in the root, we identified the MINE locus and found that the MINE gene encodes the pyridoxine 5′-phosphate (PNP)/pyridoxamine 5′-phosphate (PMP) oxidase, PDX3. Our results revealed that MINE/PDX3 likely plays a role in the conversion of the auxin precursor tryptophan to indole-3-pyruvic acid in the auxin biosynthesis pathway, in which TAA1 (TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1) and its related genes (TRYPTOPHAN AMINOTRANSFERASE RELATED 1 and 2; TAR1 and TAR2) are involved. Considering that TAA1 and TARs belong to a subgroup of PLP (pyridoxal-5′-phosphate)-dependent enzymes, we propose that PLP produced by MINE/PDX3 acts as a cofactor in TAA1/TAR-dependent auxin biosynthesis induced by ethylene, which in turn influences the crosstalk between ethylene and auxin in the Arabidopsis root.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제20권5호
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• pp.56-65
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• 2019

Recently, automobiles have been developed for safety and environmental reasons. Particularly, awareness of automobile safety is changing significantly. As a result, safety systems developed by ADAS have emerged. However, the period of mass production through ADAS development and test evaluation is long. Therefore, in this paper, we develop a brake dynamometer to shorten the time required for ADAS development and test evaluation. In addition, the developed brake dynamometer satisfies the international standard JIS D-0210, and the user can evaluate the braking force by selecting test conditions and test method for each mode of ADAS. We use the ACC, LKAS, and AEB scenarios proposed in previous studies to verify the reliability of the developed brake dynamometer. The developed brake dynamometer was verified by comparing the test values and the calculated values using theoretical formulas of the proposed ADAS mode based on previous studies. In addition, it is expected that the performance evaluation of brake parts for each ADAS mode will be possible in an environment where the vehicle test of ADAS is not possible in the future.