• Parasites, Hosts and Diseases
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• v.44 no.2 s.138
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• pp.133-138
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• 2006

This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and $0.00625{\mu}l/cm2$) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of $0.025{\mu}l/cm^2$, which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at $0.025{\mu}l/cm^2$, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.249-253
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• 1997

The acaricidal and ovicidal activity against red spider mite (Tetranychus urticae) was examined for a series of S-aryl arenethiosulfonates and compared with sulfonate acaricide, ovex and senile. Substituted diphenyl disulfides were obtained from substituted thiophenols, chloronitrobenzene and aniline derivatives. The S-aryl arenesulfonothioates were synthesised from the oxidation of disulfides. Three kind of para substituted compounds were found to have good ovicidal effect but acaricidal activity against adult mite was negligible in all S-aryl arenethiosulfonates.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.105-108
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• 2015

This research is aimed at evaluating the potential abilities of the natural acaricide of F. suspense oil against Tyrophagus putrescentiae and Dermatophagoides spp. Based on the $LD_{50}$ values, in contact bioassay, F. suspense oil (8.19, 3.28, and $4.35{\mu}g/cm^2$) showed acaricidal effects against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively. Fumigant toxicities of F. suspense oil showed similar patterns as those observed with contact toxicities. GC/MS analysis showed the major components of F. suspense oil to be ${\beta}$-pinene (45.88%), myrtenol (13.86%), (+)-${\alpha}$-pinene (13.09%), (-)-trans-pinocarveol (7.34%), sabinene (6.64%) and pinocarvone (4.13%). These findings indicate that F. suspense oil has potential as a natural acaricide.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1591-1596
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• 2006

Acaricidal effects of materials derived from Caesalpinia sappan heartwoods against Dermatophagoides farinae and D. pteronyssinus were assessed and compared with those evidenced by commercial benzyl benzoate and DEET. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the methanol extracts derived from C. sappan heartwoods were 6.13 and $5.44{\mu}g/cm^3$ against D. farinae and D. pteronyssinus, respectively. Furthermore, the ethyl acetate fraction derived from the methanol extract was approximately 8.71 more toxic than DEET against D. farinae, and 4.73 times more toxic against D. pteronyssinus. The biologically active constituent from the ethyl acetate fraction of C. sappan heartwood extract was purified via silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by $GC-MS,\;^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C\;COSY-NMR$, and DEPT-NMR spectroscopy, and identified as juglone (5-hydroxy-l,4-naphthoquinone). Based on the $LD_{50}$ values of juglone and its derivatives, the most toxic compound against D. farinae was juglone ($0.076{\mu}g/cm^3$), followed by benzyl benzoate ($9.143{\mu}g/cm^3$) and 2methyl-l,4-naphthoquinone ($40.0{\mu}g/cm^3$). These results indicate that the acaricidal activity of C. sappan heartwoods is likely to be the result of the effects of juglone. Additionally, juglone treatment was shown to effect a change in the color of the cuticles of house dust mites, from colorless-transparent to dark brownish-black. Accordingly, as a naturally occurring acaricidal agent, C. sappan heartwood-derived juglone should prove to be quite 'useful as a potential control agent, lead compound, and house dust mite indicator.

• Korean journal of applied entomology
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• v.60 no.3
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• pp.315-322
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• 2021

This study investigated the acaricidal and repellent activities of various plant extracts and Cnidium officinale-derived materials against Haemaphysalis longicornis. Among the 24 methanolic plant extracts, C. officinale showed the highest acaricidal activity (93.3%) against H. longicornis. Platycodon grandiflorus, Phytolacca americana, Magnolia denudata, Houttuynia cordata, C. officinale, and Pueraria montana showed significant differences in their repellent activity. Consequently, the activity of solvent-fractionated materials and the derived material of the C. officinale, was investigated and a 90% acaricidal effect was observed three days after hexane fraction treatment, indicating that the repellent response was statistically significant. Butylidenephthalide, which is a C. officinale-derived compound, showed 90% acaricidal activity seven days after treatment. Additionally, all H. longicornis moved to the untreated area in the t-tube olfactometer experiment, confirming the repellent activity of butylidenephthalide. Therefore, the results of this study suggest the use of C. officinale crude extract and butylidenephthalide as potent acaricidals and repellents against H. longicornis, respectively.

• Korean journal of applied entomology
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• v.47 no.3
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• pp.249-254
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• 2008

This study was conducted to develop an organic control method of two spotted spider mite(Tetranychus urticae) by using cooking oil and yolk mixture(COY) through evaluating its acaricidal activity in laboratory and green house. In laboratory, there is no significant difference in acaricidal activity against T. urticae within the COY including soybean, canola(rape seed), sunflower and olive oil. The acaricidal activity against T. urticae. increased from 17.6% to 94.1% as the COY became concentrated between 0.1% to 1%. The COY acricidal activity was effected by the quantity of treatment according to application methods. The COY dealt with T. urticae eggs presented 95% of the ovicidal activity. In rose greenhouse damaged by T. urticae, the COY(0.3%) was sprayed three times and resulted in the high control value of mites between 69.0% to 89.6%.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.2
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• pp.135-146
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• 2020

In the strawberry cultivation areas and domestic farms, two-spotted spider mites are the most serious pests. It decreases the product yield due to the direct feeding of chlorophyll by the mites resulting in reduced photosynthetic ability of host plant. In this study, a simplified acaricidal effect evaluation system (SAEES) was employed to choose the effective acaricidal products among 10 items based on leaf-dipping bioassay methods. SAEES had the advantage of being able to screen four commercial products with three replications at the same time in the recommended concentration. The susceptible strains (SL_YS) showed a high mortality rate of about 90-100%, whereas the mortality of local strains was differed by each acaricides. It suggests that the acaricide responses of field populations might differ due to spray frequencies and acaricide product. An efficacy index (0.8-1.0) was determined based on the mortality of susceptible strain, which would allow the most effective commercial products to be selected by the range of this index. In summary, SAEES will enable the selection of effective commercial products and contribute to increasing control against T. urticae in strawberries.

• Korean Journal of Poultry Science
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• v.47 no.4
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• pp.211-217
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• 2020

This study evaluated calcium hydroxide (slaked lime) and a combination of plant extracts ('natural product'; clove, cinnamon, and saponin; 1:1:1 ratio) as acaricidal control mechanisms for poultry red mites. Red mite susceptibility was evaluated after treatments with 10% slaked lime, 20% slaked lime, and 1% natural product. The duration of the acaricidal effect was also tested at 0, 10, 30, and 60 min after treatment using 20% slaked lime, 1% natural product, or a mixture of both. In the in vitro experiment, the slaked lime treatments were 73.2% (10% slaked lime) and 85.1% (20% slaked lime) effective on red mites. In acaricidal effect of control materials over times, with 20% slaked lime, the acaricidal effect decreased to 50.7% after 30 min, and 12.7% after 60 min (P<0.05). With 1% natural product, there was no acaricidal effect after 30 min (P<0.05). With 20% slaked lime +1% natural product, all of poultry red mites died until 30 min, and 92.9% after 60 min (P<0.05). On the farm, poultry red mites were observed that the number of poultry red mites increased 7,923 from 36 to 45 weeks, but then decreased to 483 after 20% slaked lime plus 1% natural product treatment. These results indicate that combining slaked lime and plant extracts effectively control poultry red mites.

• Korean Journal of Organic Agriculture
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• v.26 no.4
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• pp.707-717
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• 2018

The acaricidal activity of extracts of Gleditsia japonica var. koraiensis, Camellia sinensis, Cinnamomum cassia, Lantana camara and their mixtures against the tea red spider mite, Tetranychus kanzawai was evaluated. Treatments with mixtures 3 and 1 were effective against the adult females of T. kanzawai and yielded 82.0% and 77.3% adulticidal activities at 7 days after treatment, respectively. Treatment with mixture 2 revealed 68.0% adulticidal activity. Generally, the acaricidal activity of the single treatment of each plant extract was lower than the mixtures. The adult females of T. kanzawai treated with mixtures 3 and 1 produced only 24.0~29.6% as many eggs as control females did. The residue of the plant extracts tested had low adulticidal activity (16.7~31.3% at 7 days after treatment). All the plant extracts tested exhibited no remarkable toxic effect to the eggs of T. kanzawai. Treatments of mixtures 3 and 1 were effective against nymphs of T. kanzawai and led to emergence rates of 16.7 and 22.0%, respectively. These results suggest that mixtures 3 and 1 might be used for the control of T. kanzawai in tea fields.

• Journal of the korean veterinary medical association
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• v.33 no.12
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• pp.743-748
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• 1997

Doramectin(DECTOMAXR), 25-cyclohexyl avermectin, which has acaricidal and anthelmintic effect was evaluated against the natural infestations of Sarcoptes scabiei var suis and GI nematodes(Ascaris suum and Trichuris suis) as measured by litter size and wei