• Applied Biological Chemistry
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• v.35 no.1
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• pp.42-50
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• 1992

We conducted screening on Chinese medicinal herbs to examine their anti-complementary activity by hemolytic complementary assay $(TCH_{50})$. Among 55 kinds of herbs, several herbs showed relatively potent anti-complementary activity which decreased $TCH_{50}$, more than 70% in comparison with control. Then, hot water extracts of the following herbs, Curcuma aromatica, Areca catechu, Gleditsiae spina, Euonymus alata, Acanthopanax senticous. Lonicera japonica, Aconitum carmichaeli, Curcuma zedoaria and Cinnamoum cassia, which were shown relatively potent anti-complementary activity were partially purified and analyzed their chemical properties. These activities were resistant to digestion with pronase but decreased by treatment with $NaIO_4$. These results may indicate that the complement activating ability in their herbs is due to polysaccharide. Furthermore, the anti-complementary activity of Areca catechu which was showed the most potent activity, was reduced partially in the absence of the $Ca^{++}\;ion$. After incubation of the normal human serum with partially purified polysaccharide of A. catechu in the absence of $Ca^{++}\;ion$, a cleavage of C3 in the serum was found to have occurred through immunoelectrophoresis using rabbit anti-human C3 serum. These results indicate that the mode of complement activation by polysaccharide of A. catechu is via both the alternative and classical pathway.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.82-93
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• 2004

Phylogenetic relationships among species in Araliaceae were analyzed using PCR-RAPD and sequence of ITS region of nuclear ribosomal DNA based on samples collected in Korea. RAPD analysis showed various polymorphic bands which were able to differentiate species and genus, and specific bands showing variations among individuals within species. Cluster analysis using gel images revealed high molecular variability within species of Aralia eleta. No significant variation was found among cultivated species of Panax ginseng, but they showed high genetic differences with wild type of the species. In ITS analysis, specific sequences for each genus and species were observed and these were allowed to differentiate species and genus. Phylogenetic analysis using ITS sequences showed that Acanthopanax and Kalopanax had a close relationship, and Aralia and Panax are monophyletic, but genus Hedera is different species from other species in family Araliaceae in this study. The results showing close relationship between genera Aralia and Panax were also observed in RAPD analysis. Contrary to the results of RAPD analysis of Panax ginseng, sequence analysis of ITS showed no significant difference between wild mountain ginseng and cultivated species of P. ginseng. Also, both RAPD and ITS analysis of P. ginseng showed no significant genetic variability among cultivation sites. Results indicate that P. ginseng cultivating in Korea is monophyletic. The molecular analysis used in this study agreed on classification using morphological feature. These results suggest that molecular techniques used in this study could be useful for phylogenetic analysis of Araliaceae.

• Korean Journal of Food Science and Technology
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• v.30 no.3
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• pp.624-629
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• 1998

This studies were carried out to investigate antimicrobial activity of 35 kinds of medicinal herbs against lactic acid bacteria isolated from home-made Kimchi. The most isolated lactic acid bacteria were inhibited by ethanol extract of various medicinal herbs such as Schizandra chinensis (SC), Salvia miltiorrhiza (SM), Glycyrrhiza uralensis (GU), Lithospermum erythrorhizon (LE) and Sophrora flavescens AITON (SF). But Cnidium monnier, Pinus densiflora, Paeonia suffruticosa, Acanthopanax sessiliflorum and Rhus chinensis inhibited the growth of only a few isolated lactic acid bacteria. The pH of Kimchi containing 1% of medicinal herbs extracts such as SM, GU, LE and SF was higher than that of control during fermentation for 25 days at $10^{\circ}C$, respectively. Titratable acidity and viable cells of total bacteria and lactic acid bacteria of the Kimchi were lower than that of control during fermentation. The sensory quality (taste, flavor and overall acceptability) of SM, GU, and LE added Kimchi was similar to that of control at 10th day of fermentation. But Sophrora flavescens AITON added Kimchi decreased significantly its sensory quality compared with control(P<0.05).

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.498-515
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• 2011

The flora of Juwangsan National Park was surveyed. The field works was done for seven times from March to October 2008. Endangered species designated by The Ministry of Environment in Juwangsan National Park were found to be Hylotelephium ussuriense (KOM.) H. OHBA, Berchemia berchemiaefolia (MAK.) KOIDZ, Jeffersonia dubia BENTH., Iris odaesanensis Y.N.LEE and Acanthopanax senticosus (RUPR. et MAX.) HARMS, Lilium cernum KOM., Thalictrum coreanum LEV.(7 taxa). The floristic special plants were recorded to a total of 88 taxa; that is, degree V species (8 family 9 taxa) were containing as Jeffersonia dubia BENTH., Berchemia berchemiaefolia (MAK.) KOIDZ, Lilium cernum KOM., etc, degree IV species (4 family 5 taxa) were Cimicifuga heracleifolia KOM., Anemone reflexa STEPH. et WILLD., Patrinia rupestris JUSS., etc, degree III species (16 family 18 taxa) were containing; Pyrrosia petiolosa (CHRIST.et BARONI) CHING, Exochorda serratifolia S. MOORE., Aristolochia manshuriensis KOM., etc, degree II species (14 family 18 taxa) were Paeonia japonica MIYABE et TAKEDA, Isopyrum raddeanum (REGEL) MAX., Cypripedium macramthum SW., etc and degree I species (30 family 38 taxa) were Botrychium virginianum (L.) SW., Chloranthus japonicus SIEB., Dictamnus dasycarpus TURCZ., etc. 15 taxa that included Salix hulteni FLODERUS, Celtis choseniana NAKAI, Clematis trichotoma NAKAI, Vicia venosissima NAKAI, etc. were found to be endemic to korea and 21 taxa that included ; Bilderdykia dumetora (L.) DUM., Bilderdykia convolvulus DUM., Lepidium apetalum WILLD., Amorpha fruticosa L. and Symphytum offcinale L., Hibiscus trionum L. etc. were identified as naturalized plants.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.410-417
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• 2014

Berries and green tea are underutilized in the food industry despite their great potential as a functional food ingredients. The purpose of this study was to determine the extraction conditions under which total phenolic contents and antioxidant activities of berry and green tea extracts are maximized. Extracts produced using 0-80% ethanol and temperatures ranging from $5-65^{\circ}C$ were evaluated for total phenolic content (TP), as well as for DPPH and ABTS radical-scavenging activities by using response surface methodology. Both ethanol and temperature had significant effects (p<0.05). Ogaja extract produced at $67^{\circ}C$ by using 33% ethanol yielded maximum TP, ABTS, and DPPH values of 23.74 mg GAE/g, 19.77, and 25.04 mg VCE/g, respectively. Optimum conditions for mulberry and raspberry extraction were found to be $65^{\circ}C$ by using 69% and 40% ethanol, respectively. Mulberry and raspberry extracts had TP, DPPH, and ABTS values of 20.74 mg GAE/g, 23.55, and 35.44 mg VCE/g, and 26.08 mg GAE/g, 39.93, and 55.60 mg VCE/g, respectively. Green tea extraction at $57^{\circ}C$ by using 43% ethanol was found to be optimal, yielding TP, ABTS, and DPPH values of 101.15 mg GAE/g, 171.38, and 177.56 mg VCE/g, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.4
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• pp.626-632
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• 2004

Of hot- water extracts prepared from 30 kinds of herbal medicines, Acanthopanax senticosus (75.6% inhibition of control), Atractylodes macrocephale (71.3%), Panax ginseng (70.0%), Glycyrrhiza uralensis (66.3%) and Angelica acutiloba (63.1%) showed the potent tumor metastasis inhibition activity against colon 26-M3.1 lung carcinoma at 2.5 mg/kg body weight, whereas the other extracts had a little activity, except for Pueraria thunbergiana (58.6%) and C. leticulata (54.9%) having the intermediate activity. We also found that Citrus leticulata (1.80-fold of control), A. macrocephale (1.73-fold), A. senticosus and G. uralensis (1.64-fold) enhanced on Peyer's patch cells mediated-hematopoietic response at 100 $\mu\textrm{g}$/mL. In addition, these active herbal medicines were prepared into steam distillates to improve the food rheology as beverage, and to remove the inactive components. Among these steam distillates, A. macrocephale, G. uralensis and A. senticosus showed the significant tumor metastasis inhibition activity at 2.5 mg/kg body weight (58.7%, 50.3% and 41.9%, respectively), and A. macrocephale had the potent activity even at 0,25 mg/kg body weight (49.7%). In treatments of steam distillates with Peyer's patch cells, A. macrocephale and A. senticosus significantly increased the bone marrow cell proliferation even at 10 $\mu\textrm{g}$/mL (1.49- and 1.28-fold of control). Although steam distillates had lower activity than hot-water extracts, herbal medicines, such as A. macrocephale and A. senticosus, showed the high immunostimulating activity in hot-water extracts as well as steam distillates. Therefore, these results assumed the possibility that steam distillates from herbal medicines might be utilized to food industry for beverage.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.7
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• pp.1057-1064
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• 2016

This study was carried out to investigate the effect of fermented herbal mixtures (FHMs) in HepG2 and PC12 cells. Two different types of fermented herbal mixtures consisted of Chrysanthemum morifolium, Ganoderma lucidum, Acanthopanax senticosus, Schisandra chinensis, Hovenia dulcis thumb, and Lycii fructus. FHM-A and FHM-B were separately fermented with Prunellae Spica, Portulaca oleracea (FHM-A) and Acorus gramineus, Pycnostelma paniculatum (FHM-B). Total phenolic content of FHM-B was higher than that of FHM-A. ORAC values in both FHM-A and FHM-B increased in a dose-dependent manner, and antioxidant activities against peroxyl radicals were higher in FHM-A than FHM-B. Both FHM-A and FHM-B effectively ameliorated AAPH- and ethanol-induced oxidative stress in HepG2 cells. They also suppressed lipid formation induced by ethanol treatment. In addition, FHM-A and FHM-B prevented $H_2O_2$-induced PC12 cell death. FHM-B showed a relatively stronger protective effect than that of FMB-A. Taken together, these findings show that a fermented herbal mixture could be used in healthy and functional food design for oxidative stress-related diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.605-610
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• 2003

According to the Leukemia and Lymphoma Society, leukemia is a malignant disease (cancer) that originates in a cell in the marrow. It is characterized by the uncontrolled growth of developing marrow cells. There are two major classifications of leukemia: myelogenous or lymphocytic, which can each be acute or chronic. The terms myelogenous or lymphocytic denote the cell type involved. Thus, four major types of leukemia are: acute or chronic myelogenous leukemia and acute or chronic lymphocytic leukemia. Leukemia, lymphoma and myeloma are considered to be related cancers because they involve the uncontrolled growth of cells with similar functions and origins. The diseases result from an acquired (not inherited) genetic injury to the DNA of a single cell, which becomes abnormal (malignant) and multiplies continuously. In the United States, about 2,000 children and 27,000 adults are diagnosed each year with leukemia. Treatment for cancer may include one or more of the following: chemotherapy, radiation therapy, biological therapy, surgery and bone marrow transplantation. The most effective treatment for leukemia is chemotherapy, which may involve one or a combination of anticancer drugs that destroy cancer cells. Specific types of leukemia are sometimes treated with radiation therapy or biological therapy. Common side effects of most chemotherapy drugs include hair loss, nausea and vomiting, decreased blood counts and infections. Each type of leukemia is sensitive to different combinations of chemotherapy. Medications and length of treatment vary from person to person. Treatment time is usually from one to two years. During this time, your care is managed on an outpatient basis at M. D. Anderson Cancer Center or through your local doctor. Once your protocol is determined, you will receive more specific information about the drug(s) that Will be used to treat your leukemia. There are many factors that will determine the course of treatment, including age, general health, the specific type of leukemia, and also whether there has been previous treatment. there is considerable interest among basic and clinical researchers in novel drugs with activity against leukemia. the vast history of experience of traditional oriental medicine with medicinal plants may facilitate the identification of novel anti leukemic compounds. In the present investigation, we studied 31 kinds of anti leukemic medicinal plants, which its pharmacological action was already reported through many experimental articles and oriental medical book: 『pharmacological action and application of anticancer traditional chinese medicine』 In summary: Used leukemia cellline are HL60, HL-60, Jurkat, Molt-4 of human, and P388, L-1210, L615, L-210, EL-4 of mouse. 31 kinds of anti leukemic medicinal plants are Panax ginseng C.A Mey; Polygonum cuspidatum Sieb. et Zucc; Daphne genkwa Sieb. et Zucc; Aloe ferox Mill; Phorboc diester; Tripterygium wilfordii Hook .f.; Lycoris radiata (L Her)Herb; Atractylodes macrocephala Koidz; Lilium brownii F.E. Brown Var; Paeonia suffruticosa Andr.; Angelica sinensis (Oliv.) Diels; Asparagus cochinensis (Lour. )Merr; Isatis tinctoria L.; Leonurus heterophyllus Sweet; Phytolacca acinosa Roxb.; Trichosanthes kirilowii Maxim; Dioscorea opposita Thumb; Schisandra chinensis (Rurcz. )Baill.; Auium Sativum L; Isatis tinctoria, L; Ligustisum Chvanxiong Hort; Glycyrrhiza uralensis Fisch; Euphorbia Kansui Liou; Polygala tenuifolia Willd; Evodia rutaecarpa (Juss.) Benth; Chelidonium majus L; Rumax madaeo Mak; Sophora Subprostmousea Chunet T.ehen; Strychnos mux-vomical; Acanthopanax senticosus (Rupr.et Maxim.)Harms; Rubia cordifolia L. Anti leukemic compounds, which were isolated from medicinal plants are ginsenoside Ro, ginsenoside Rh2, Emodin, Yuanhuacine, Aleemodin, phorbocdiester, Triptolide, Homolycorine, Atractylol, Colchicnamile, Paeonol, Aspargus polysaccharide A.B.C.D, Indirubin, Leonunrine, Acinosohic acid, Trichosanthin, Ge 132, Schizandrin, allicin, Indirubin, cmdiumlactone chuanxiongol, 18A glycyrrhetic acid, Kansuiphorin A 13 oxyingenol Kansuiphorin B. These investigation suggest that it may be very useful for developing more effective anti leukemic new dregs from medicinal plants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1529-1533
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• 2008

The detection characteristics of gamma-irradiated ($0{\sim}10.0\;kGy$) medicinal herbs (Platycodon grandiflorum, Acanthopanax chiisanensis) were investigated by photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR). The results of the PSL, a first screening method in comparison with the TL, showed photon counts greater than 5,000 counts/60 s (positive) in the irradiated samples, while the non-irradiated samples yielded photon counts less than 700 counts/60 s (negative). The TL was also applied for the detection method of irradiated medicinal herbs and showed that the non-irradiated sample revealed a glow curve with a low intensity, while the irradiated samples showed a higher intensity. These results were normalized by re-irradiating the mineral grains with a irradiation dose of 1.0 kGy, and a second glow curve was recorded. The ratio of the intensity of the first glow curve ($TL_1$) to that after the normalization dose ($TL_2$) was determined and compared with the recommended threshold values. TL ratio ($TL_1/TL_2$) was below 0.007 for the non-irradiated sample and higher than 0.1 for all irradiated samples (above 1.0 kGy). ESR spectroscopy revealed specific signals (6.065 mT) derived from free radicals in cellulose containing irradiated medicinal herbs. The P. grandiflorum showed clearer signals than A. chiisanensis. From the results of our studies, the PSL, TL, and ESR determinations were found to be suitable for the detection of irradiated medicinal herbs such as P. grandiflorum and A. chiisanensis.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.501-512
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• 2005

In Exp. 1, a total of forty eight multiparous sows were used in a 21-d performance assay. All litters were standardized at eleven piglets within 24h of parturition. Sows were fed one of the four experimental diets 1) CON(basal diet; Control), 2) MP1(basal diet added 0.05% of medicinal plant mixtures), 3) MP2 (basal diet added 0.1% of medicinal plant mixtures) and 4) MP3(basal diet added 0.2% of medicinal plant mixtures). Backfat thickness difference from farrowing to weaning was tended to increase in CON treatment compared to those of medicinal plants mixture(MP) treatments. However, there were not significant differences among the treatments(P>0.05). Sow’s ADFI was increased in sows fed MP2 treatment compared to MP3 treatment(P<0.05). Piglet weight gain was tended to increase in MP1 treatment compared to CON treatment(P<0.05). The glucose concentration of MP3 treatment was higher that of CON treatment(P<0.05). Exp. 2, a total of one hundred twenty weaning pigs ($L{\time}Y{\time}D$, 4.70$\pm$0.63kg average initial body weight) were used in a 20-d performance assay. Weanling pigs were fed treatments diets included 1) CON(basal diet; Control), 2) MP0.05(basal diet added 0.05% of medicinal plant mixtures), 3) MP0.1(basal diet added 0.1% of medicinal plant mixtures) and 4) MP0.15(basal diet added 0.15% of medicinal plant mixtures). Through entire experimental period, as MP increased in the diets, there was an increase in ADG(linear, P<0.067), improvement gain/feed(linear, P<0.018) and a decrease in ADFI (linear, P<0.008). DM digestibility was significantly increased with addition of MP(linear, P<0.004; quadratic, P<0.030). In conclusion, sows fed MP2 had showed less body weight loss and increased weight gain for suckling piglet. In weaning pigs, as supplementation MP from 0.05 to 0.1% in diet had improved growth performance and DM digestibility.