Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Detection Characteristics of Gamma-Irradiated Korean Medicinal Herbs by Using PSL, TL, and ESR

PSL, TL 및 ESR 분석에 의한 감마선 조사 한약재의 검지 특성

  • Yang, Hee-Sun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Yong-Dae (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jin, Chang-Hyun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Dae-Seong (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chung, Hyung-Wook (New Hazard Chemicals Team, Korea Food & Drug Administration) ;
  • Byun, Myung-Woo (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jeong, Il-Yun (Radiation Research Center for Innovative Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 양희선 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 박용대 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 진창현 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 최대성 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 정형욱 (식품의약품안전청 신종유해물질팀) ;
  • 변명우 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부) ;
  • 정일윤 (한국원자력연구원 정읍 방사선과학연구소 방사선전략기술개발부)
  • Published : 2008.11.28
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Abstract

The detection characteristics of gamma-irradiated ($0{\sim}10.0\;kGy$) medicinal herbs (Platycodon grandiflorum, Acanthopanax chiisanensis) were investigated by photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR). The results of the PSL, a first screening method in comparison with the TL, showed photon counts greater than 5,000 counts/60 s (positive) in the irradiated samples, while the non-irradiated samples yielded photon counts less than 700 counts/60 s (negative). The TL was also applied for the detection method of irradiated medicinal herbs and showed that the non-irradiated sample revealed a glow curve with a low intensity, while the irradiated samples showed a higher intensity. These results were normalized by re-irradiating the mineral grains with a irradiation dose of 1.0 kGy, and a second glow curve was recorded. The ratio of the intensity of the first glow curve ($TL_1$) to that after the normalization dose ($TL_2$) was determined and compared with the recommended threshold values. TL ratio ($TL_1/TL_2$) was below 0.007 for the non-irradiated sample and higher than 0.1 for all irradiated samples (above 1.0 kGy). ESR spectroscopy revealed specific signals (6.065 mT) derived from free radicals in cellulose containing irradiated medicinal herbs. The P. grandiflorum showed clearer signals than A. chiisanensis. From the results of our studies, the PSL, TL, and ESR determinations were found to be suitable for the detection of irradiated medicinal herbs such as P. grandiflorum and A. chiisanensis.

한약재의 방사선 조사여부를 확인하기 위해 도라지와 오가피에 $0{\sim}10\;kGy$의 감마선을 조사하고 PSL, TL 및 ERS 특성을 비교 검토하였다. PSL을 측정한 결과 비 조사된 한약재에서 한계치($T_1$)인 700보다 낮은 photon count 값을 보이면서 negative로 판정되었고, 0.15 kGy 이상 조사구는 한계치($T_2$)인 5,000보다 높은 photon count 값을 나타내면서 positive로 표시되어 방사선 조사된 것을 확인할 수 있었다. 또한 시료로부터 mineral을 분리하여 TL의 glow curve와 TL ratio($TL_1/TL_2$)를 확인하였다. 방사선 조사가 되지 않은 도라지와 오가피는 TL ratio가 0.007 이하의 값을 나타내었으며, 조사구(1.0 kGy 이상)에서는 0.314 이상의 TL ratio를 나타내었다. 도라지와 오가피는 본 연구에서 설정한 TL ratio의 한계치 0.1보다 높은 수치를 나타내어 방사선 조사여부 확인이 가능하였다. ESR 측정을 통한 방사선 조사 한약재 검지에서는 cellulose 라디칼에서 유래된 특이한 라디칼 신호를 보여주었고, 이는 도라지 시료에서 더욱 뚜렷하게 확인할 수 있었다. 이상의 결과에서 PSL, TL 및 ESR 분석은 도라지와 오가피의 방사선 조사 여부 확인에 적합한 방법임을 확인할 수 있었다

Keywords

References

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