• Journal of the Korean Society for Library and Information Science
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• v.41 no.4
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• pp.135-160
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• 2007

This study aims to raise academic library funds in Korea through how to raise funds and what conditions are. In order to do this, case studies on academic library funds of university in America as well as academic library funds of university in Korea have been analyzed. As for the specific plan for raising academic library funds, I have indicated to establish office for library development and include library as one of allotment for infrastructure which is similar to the Development Impact Fee in the U.S.A., and the use of library supporting organizations like "Library Friends."

• Journal of Veterinary Science
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• v.22 no.4
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• pp.45.1-45.13
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• 2021

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 10⁶ cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

• Korean Institute of Interior Design Journal
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• v.14 no.6 s.53
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• pp.47-56
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• 2005

The purpose of this paper is to understand the meaning of 'institution' in Louis Kahn's architecture and to reveal that it is expressed through singularity and commonality, which have two aspects; formal-material(eidos-hyle) aspect and phenomenological aspect. Under these recognitions, this paper shows the detail analysis on design process of Phillips Exeter Academy Library, focusing on his writings and sketches. The results are as follows. First, the basic form is maintained through the design process of the Exeter library, and it is derived from the consideration of the human activities in the library. And second, each 'room' of the library acquires its singularity through structure and light. And the 'plan' is based on the commonality inherent in the singularity of each room. And the last, singularity and commonality are revealed not only In the relation of architectural elements but also In the relation with man and architecture.

• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1196-1206
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• 2014

A metagenomic fosmid library was constructed using genomic DNA isolated from the gut microflora of Hermetia illucens, a black soldier fly. A cellulase-positive clone, with the CS10 gene, was identified by extensive Congo-red overlay screenings for cellulase activity from the fosmid library of 92,000 clones. The CS10 gene was composed of a 996 bp DNA sequence encoding the mature protein of 331 amino acids. The deduced amino acids of CS10 showed 72% sequence identity with the glycosyl hydrolase family 5 gene of Dysgonomonas mossii, displaying no significant sequence homology to already known cellulases. The purified CS10 protein presented a single band of cellulase activity with a molecular mass of approximately 40 kDa on the SDS-PAGE gel and zymogram. The purified CS10 protein exhibited optimal activity at $50^{\circ}C$ and pH 7.0, and the thermostability and pH stability of CS10 were preserved at the ranges of $20{\sim}50^{\circ}C$ and pH 4.0~10.0. CS10 exhibited little loss of cellulase activity against various chemical reagents such as 10% polar organic solvents, 1% non-ionic detergents, and 0.5 M denaturing agents. Moreover, the substrate specificity and the product patterns by thin-layer chromatography suggested that CS10 is an endo-${\beta}$-1,4-glucanase. From these biochemical properties of CS10, it is expected that the enzyme has the potential for application in industrial processes.

• Journal of Information Science Theory and Practice
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• v.8 no.1
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• pp.45-55
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• 2020

The purpose of this work was to study library website users' actions by tracking their behavior, determining popular content, and identifying browsing patterns and subsequent improvement of access to popular content. The study of behavior models and the use of web analytics has led to the emergence of solutions that improve the usability and functionality of the State Public Scientific-Technological Library of the Siberian Branch of the Russian Academy of Sciences (SPSTL SB RAS) website. These are: identifying user tasks as they are developed, conducting user testing to better understand the event. tracking data and collecting additional data to verify the effectiveness of the changes made. Examining data on the duration of the session and the number of visits will help determine the goals of user visits and develop new recommendations. Usability analysis and testing will make it possible to compare the data obtained using web analytics and the perception of the library site by the users themselves. Recommendations are offered to libraries on the use of data on the real behavior of the target audience of the library website to improve access to library resources and services, increase their relevance and improve information services.

• Journal of Korean Library and Information Science Society
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• v.33 no.1
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• pp.143-167
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• 2002

The aim of this study is to examine the influence of foreign library classification schemes on the Chinese classification systems in the library. And the study is to analyze development process of three modem library classification schemes, the $\ulcorner$Renmin University of China's Library Books Classifications$\lrcorner$, $\ulcorner$Chinese Academy of Sciences's Library Books Classifications$\lrcorner$and $\ulcorner$Chinese Library Classification$\lrcorner$which are being used in many libraries in China where the library is regarded an important organization for performing the national policies.

• Korean Institute of Interior Design Journal
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• v.14 no.5 s.52
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• pp.89-97
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• 2005

The purpose of this study is to analyze the interior characteristics of Philips Exeter Academy library by Louis I. Kahn. Kahn's main thoughts of 'what it wants to be ' and 'how it was done' play a important role in this research because these statements act as a clue to understand this library. 'What it wants to be' means the essence and existence of architecture and 'how it was done' shows the tectonic of architecture and construction process to enhance its presence. Most of his works inform that Kahn had consistently applied his thoughts into design process. The thoughts of the essence of library leads Kahn to configurate 3 layers of interior space. The emphasis of tectonic expression is also the characteristics of interior in that structure is the logical part of whole building system rather than concealing object for the exterior of buildings. In Exeter library, interior characteristics are summarized as 3 layers of interior configuration, the order in dynamics, the visualization of construction process, and the relationship between structure and space.

• Journal of the Korean Society for Library and Information Science
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• v.28
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• pp.193-245
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• 1995

New curriculum for information and library science is proposed on the notion of the internship and scholarly characteristics. The future of the information and library science is expected as the discipline to synthesize the natural science and the social science. Especially the internship is strongly suggested as the post graduate program. To draw the conclusion. the history of the information and library science and its educational background are analyzed. The states of the historical developments of the information and library science are explained in terms of philosophy. The functional and scholarly environments of the information and library science are also investigated. Secondarily it concludes that the curriculum development for information and library science is not aimed on the skills but on the phenomena.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.888-897
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• 2009

Lipase diversity in glacier soil was assessed by culture-independent metagenomic DNA fragment screening and confirmed by cell culture experiments. A set of degenerate PCR primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly PCR amplify metagenomic DNA from glacier soil. These products were used to construct a lipase fragment clone library. Among the 300 clones sequenced for the analysis, 201 clones encoding partiallipases shared 51-82% identity to known lipases in GenBank. Based on a phylogenetic analysis, five divergent clusters were established, one of which may represent a previously unidentified lipase subfamily. In the culture study, 11 lipase-producing bacteria were selectively isolated and characterized by 16S rDNA sequences. Using the above-mentioned degenerate primers, seven lipase gene fragments were cloned, but not all of them could be accounted for by the clones in the library. Two full-length lipase genes obtained by TAIL-PCR were expressed in Pichia pastoris and characterized. Both were authentic lipases with optimum temperatures of ${\le}40^{\circ}C$. Our study indicates the abundant lipase diversity in glacier soil as well as the feasibility of sequence-based screening in discovering new lipase genes from complex environmental samples.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1484-1489
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• 2014

A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at $10^{\circ}C$ (43% activity remained), with the optimal temperature at $20^{\circ}C$, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.