• Journal of Engineering Education Research
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• v.13 no.5
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• pp.41-48
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• 2010

In this paper, we proposed "Future Design and Success Engineering in Global Society by Self-Innovation" (an abbreviated name: FDSE). We started to make a small experiment between Korea Maritime University and Yamagata University at the current year. After inspecting the results, especially, successful and unsuccessful points in our lecture, we have to develop the strong points and amend the week points, through making the analysis of success-failure, which is his favorite management method. We have to level up the contents of our work in the future. The students for this lecture would learn from experiences of "the common knowledge (common field of discussion)", held common languages, common thought, common system. They filled up the blanks of "The Matrix Frame" which is mentioned in the previous report, and they expressed their own way of life (work, study). As a matter of course, their personal private affairs must not be made public. It is quite happy that the cultural, economical exchanges between Korea and Japan are now in full flourish and people do a lot of traveling each other. However, if this project will start on the educational global standard, we have a world-wide significance. Moreover, we can promote goodwill between Korea and Japan, and also we can contribute to a better international understanding and the peace of the world by students in both Universities. We hope that candidates for this lecture will have a rounded personality and be a life worthy of human being for realizing Self-Actualization in "Motivation and Personality" by Abraham Maslow.

• Mycobiology
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• v.43 no.2
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• pp.137-149
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• 2015

In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration ($LC_{50}$) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

• Natural Product Sciences
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• v.26 no.3
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• pp.207-213
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• 2020

Characterization and in vitro inhibition studies of protease inhibitor from the mushroom Pleurotus floridanus (PfTI) towards the pest Papilio demoleus is studied. The addition of 1 mM Mn²⁺, Na²⁺, Ba²⁺ and Ni ²⁺ enhanced the PfTI activity. The ICP-atomic emission spectrum showed the presence of Ca²⁺, Mg²⁺ and Zn²⁺ in the PfTI. Surfactants SDS and CTAB at a concentration of 1% reduced the PfTI activity whereas, the nonionic detergents Triton X and Tween 80 increased the activity. The inhibitory activity gradually decreased with increase in concentration of DMSO and H₂O₂. The activity was increased by dithiothreitol up to a concentration of 80 μM and inactivated at 140 μM. The activity of PMSF modified PfTI was drastically reduced to 0.234 U/mL at 4 mM concentration and similar results were obtained for modification of cysteine by N-Ethylmaleimide at slightly higher concentrations. The complex of trypsin and PfTI showed complete loss in fluorescence intensity at 343 nm compared with control. In vitro inhibition studies of PfTI with midgut proteases isolated from citrus pest P. demoleus with protease activity of 1.236 U was decreased to 0.613 U by 50 μL (0.1 mg/mL) of the inhibitor. Inhibitor was stable up to 0.04 M concentration of HCl.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.806-812
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• 2003

Attempts have been made to establish relationship between the response to ACTH challenge in female calves, growth and first lactation performance. A total of 19 Holstein calves weighing 100 kg i. v. were given 0.50 IU of ACTH/kg $BW^{.75}$ (EXACTHIN inj., Richter G., Budapest) at 60 days of age. Serial blood samples were taken at times 0, 0.5, 1, 2, 3, 4 and 5 hours and analyzed for cortisol, glucose insulin and FFA levels. From challenge series the area under the curve from time of administration and the following 5 h were calculated. Negative, and mostly loose relationship between response to ACTH challenge for cortisol, insulin, or FFA and ADWG during growth have been established (p>0.05) with positive one for glucose. Bivariate coefficients of correlation varied within the range from -0.35 to 0.15. Estimations reveal negative correlation between the length of first lactation and cortisol or insulin (r=-0.80, p<0.001 and r=-0.45, p<0.10, resp.) Close association between cortisol or insulin and actual first lactation milk yield was found (r=-0.48, p<0.10; r=-0.64, p<0.01, resp.). Close relationship between the response to ACTH challenge and milk protein yield was present only for insulin (r=-0.59, p<0.05).

• Microbiology and Biotechnology Letters
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• v.40 no.4
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• pp.303-309
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• 2012

Keratinases are of particular interest because of their action on insoluble keratins and generally on a broad range of protein substrates. Alkalophilic and neutrophilic actinomycete strains isolated from different soil samples, rich in keratinaceous substances were screened for keratinolytic activity. An alkalophilic isolate, TBG-S13A5, was found to possess good keratinolytic activity and was able to utilize feather as the sole carbon and nitrogen source. TBG-S13A5 exhibited an off-white aerial mass color with a rectus-flexibilis type of spore chain. The morphological, microscopical and biochemical characters were comparable with that of Streptomyces albidoflavus. Fatty acid methyl ester profiling (FAME) and 16S rDNA sequence analysis confirmed its identity as a strain of S. albidoflavus. Under submerged fermentation conditions, maximum protease production was recorded on the $5^{th}$ day of incubation at $30^{\circ}C$, using basal broth of pH 9.0 with 0.25% (w/v) white chicken feather. This strain could affect feather degradation when the initial pH was 8 and above and maximum protease production was recorded when the initial pH was around 10.5. The effectiveness of the crude enzyme in destaining and leather dehairing were also demonstrated.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1862-1867
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• 2007

An individual's immune response is critical for host protection from many different pathogens, and the responsiveness can be assessed by the amount of cytokine production upon stimulating bacterial components such as lipopolysaccharide (LPS). The difference between individuals in their peripheral blood mononuclear cells (PBMC) responsiveness to LPS, a Gram-negative endotoxin, was investigated from 27 healthy individuals. We observed a large variation in $IFN{\gamma}$ production among different individuals. The PBMC of the consistently three highest and three lowest $IFN{\gamma}$ producers were investigated. Since previous studies described that a single point mutation in the coding region of TLR2 and TLR4 is linked to the individual responsiveness to pathogenic bacterial infections, we first examined the known point mutations in the coding region of $TLR2^{Pro681His}$, $TLR4^{Pro714His}$ located in the cytoplasmic regions of the Toll-like domain as well as $TLR4^{Asp299Gly}$ located in the extracellular region. None of these mutations were associated with an individual's responsiveness to LPS, despite the presence of $TLR4^{Asp299Gly}$ mutation. Further investigation revealed that the variation of PBMC responsiveness to LPS among healthy individuals was due to constitutive expression levels of TLR4 and TLR2. This result is consistent with an aging-related low expression of Toll-like receptors in the mouse model of LPS responsiveness. The present study therefore suggests that the constitutive expression levels of TLR2 and TLR4 may contribute to the individual response to LPS.

• Journal of the Korean Society of Costume
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• v.19
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• pp.175-194
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• 1992

Le Proche-Orient est le pays de la Bible, Le peuple He breu ancien, appartenant la race des S mits s mi-nomades, est venu avec Abraham, de m sopotamie en Palestine, terre de Canaan. Nous avons consid r les v tements du peuple des anciens H breux en nous basant sur la Bible et en observant des peintures murales et des bas-reliefs des divers payes de l'ancienne poque, Pour comprendre l' volution des v tements des gens de la pr sente r gion palestinienne, nous avons tudi les v tements traditionnels de la race b douine qui m nent jusqu' maintenant une vie nomade dans le m nent jusqu' maintenant une vie nomade dans le d sert, parce que nous n'avons pas pu connaitre le processus d' volution des v tements apr s l'ancienne poque la suite d' v nements historiques compliques. En conclusion, nous pouvons nous r sumer comme suit: 1) Nous pensons que la kimlah, costume important du peuple h breu ancien est devenu abajeh, manteau des B douins, puisque ces v tements servent prot ger le corps lors des changments de temps, et la nuit on les utilise comme couverture et comme sac pour y mettre des objets, et comme tente lors su travail. en un mot, les fonctions de la Simlah et celles de l'abajeh sont les memes l' poque ancienne et maintenant. 2) Nous pensons que la forme et l'utilisation de la Kethoneth ayant une forme de tunique et de la thob des B douins sont presque semblable. La kethoneth et la thob sont la tuniqu importante que portent jusqu' maintenant tous les peuples du Proche-Orient. 3) Comme on le voit dans la Bible, les femmes du peuple h breu et celles des B douins utilisent le voile pour couvrir la e te, et se servent d'accessoires pour d corer leur corps. A l'avenir, les vVtements des Palestiniens, dans une recherche pous approfondie, feront l'objet de a 2 me partie de l' tude des costumes du Proche-Orient.

• Advances in Energy Research
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• v.2 no.1
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• pp.33-45
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• 2014

Photocatalytic hydrogen generation by water splitting ($H_2O_{(1)}{\rightarrow}H_2_{(g)}+1/2O_2_{(g)}$) has been studied on photocatalysts based on Zn, Cd, Fe and Cu, synthesized by coprecipitation. Iron and copper nanoparticles were incorporated as cocatalysts to enhance the photocatalytic activity of the ZnCd solid solution. The effect of the different synthesis parameters (temperature, elemental atomic ratios, amount of Cu and Fe incorporated in the catalyst and calcination temperature) on the photocatalytic production of hydrogen has been studied in order to determine the best experimental synthesis conditions. The catalysts have been characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and BET. The experiments of photocatalytic water splitting were performed in aqueous solution of the photocatalysts previously dispersed in a soft ultrasound bath. The photocatalysts were irradiated under different lights ranging from 220 to 700 nm. The photocatalytic activity was found to be clearly dependent on the specific area of the photocatalyst.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6109-6113
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• 2012

Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective: This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and Methods: Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result: From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion: The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.1
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• pp.219-223
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• 2016

Background: Promoter hypermethylation is a frequent epigenetic mechanism for gene transcription repression in cancer and is one of the hallmarks of the disease. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) contributes to cell contact-mediated communication. Dysregulation of promoter methylation has been reported in various cancers. Objectives: The objectives of this study were to investigate the CELSR3 hypermethylation level in oral squamous cell carcinomas (OSCCs) using methylation-sensitive high-resolution melting analysis (MS-HRM) and to correlate CELSR3 methylation with patient demographic and clinicopathological parameters. Materials and Methods: Frozen tissue samples of healthy subjects' normal mucosa and OSCCs were examined with regard to their methylation levels of the CELSR3 gene using MS-HRM. Results: MS-HRM analysis revealed a high methylation level of CELSR3 in 86% of OSCC cases. Significant correlations were found between CELSR3 quantitative methylation levels with patient ethnicity (P=0.005), age (P=0.024) and pathological stages (P=0.004). A moderate positive correlation between CELSR3 and patient age was also evident (R=0.444, P=0.001). Conclusions: CELSR3 promoter hypermethylation may be an important mechanism involved in oral carcinogenesis. It may thus be used as a biomarker in OSCC prognostication.