Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

  • Goot-Heah, Khor (Centre of Studies for Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA) ;
  • Kwai-Lin, Thong (Institute of Biological Science, Faculty of Science, University of Malaya) ;
  • Froemming, Gabriele Ruth Anisah (Institute for Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA) ;
  • Abraham, Mannil Thomas (Tengku Ampuan Rahimah Hospital, Department of Oral and Maxillofacial Surgery, Ministry of Health Malaysia) ;
  • Rosdy, Nik Mohd Mazuan Nik Mohd (Centre of Studies for Oral and Maxillofacial Diagnostic and Medicine, Faculty of Dentistry, Universiti Teknologi MARA) ;
  • Zain, Rosnah Binti (Oral Cancer Research and Coordinating Centre (UM-OCRCC), Faculty of Dentistry, University of Malaya)
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective: This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and Methods: Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result: From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion: The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.

Keywords

References

  1. An HJ, Cho NH, Lee SY, et al (2003). Correlation of cervical carcinoma and precancerous lesions with human papillomavirus (HPV) genotypes detected with the HPV DNA chip microarray method. Cancer, 97, 1672-80. https://doi.org/10.1002/cncr.11235
  2. Avon SL (2004). Oral mucosal lesions associatedwith use of quid. J Can Dent Assoc, 70, 244-8
  3. Badaracco G, Venuti A, Bartolazzi A, et al (2000). Overexpression of p53 and bcl-2 proteins and the presence of HPV infection are independent events in head and neck cancer. J Oral Pathol Med, 29, 173-9. https://doi.org/10.1034/j.1600-0714.2000.290405.x
  4. Bouda M, Gorgoulis VG, Kastrinakis NG, et al (2000). High risk HPV types are frequently detected in potentially malignant and malignant oral lesions, but not in normal. Oral Mucosa Mod Pathol, 13, 644-53.
  5. Dissemond J (2004). Oral lichen planus: an overview. J Dermatol Treat, 15, 136-40. https://doi.org/10.1080/09546630410030720
  6. Dokianakis DN, Spandidos DA (2000). P53 codon 72 polymorphism as a risk factor in the developmentof HPV-associated cervical cancer. Mol Cell Biol Res Commun, 3, 111-4. https://doi.org/10.1006/mcbr.2000.0196
  7. D'Souza G, Kreimer AR, Viscidi R, et al (2007). Case-control study of human papillomavirus and oropharyngeal cancer. N Engl J Med, 356, 1944-56. https://doi.org/10.1056/NEJMoa065497
  8. Duensing S, Lee LY, Duensing A, et al (2000). The human papillomavirus type 16 E6 and E7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle. PNAS, 97, 10002-7. https://doi.org/10.1073/pnas.170093297
  9. Duensing S, Munger K (2004). Mechanisms of genomic instability in human cancer: insights from studies with human papillomavirus oncoproteins. Int J Cancer, 109, 157-62. https://doi.org/10.1002/ijc.11691
  10. Esquenazi D, Bussoloti FI, Carvalho MG, et al (2010). The frequency of human papillomavirus findings in normal oral mucosa of healthy people by PCR. Braz J Otorhinolaryngol, 76, 78-84.
  11. Fuju C, Stina S, Jari K, et al (1991). Human papillomavirus (HPV) infections and their associations with oral disease. J Oral Pathol and Med, 20, 305-17. https://doi.org/10.1111/j.1600-0714.1991.tb00936.x
  12. Gichki AS, Buajeeb W, Doungudomdacha S, et al (2012). Detection of human papillomavirus in normal oral cavity in a group of pakistani subjects using real-time PCR. Asian Pac J Cancer Prev, 13, 2299-304. https://doi.org/10.7314/APJCP.2012.13.5.2299
  13. Iftner T, Villa LL (2003). Human papillomavirus technologies. J Natl Cancer Inst Monogr, 31, 80-8
  14. Joao M, Frederico SR, Gustavo J, et al (2006). Oral squamous cell carcinoma: Review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102, 67-76. https://doi.org/10.1016/j.tripleo.2005.07.038
  15. Khovidhunkit SP, Buajeeb W, Sanguansin S, et al (2009). Detection of human papillomavirus in oral squamous cell carcinoma, leukoplakia and lichen planus in Thai patients. Asean Pac J Cancer Prev, 9, 771-5.
  16. Kulkarni SS, Kulkarn SS, Vastrad PP, et al (2011). Prevalence and distribution of high risk human papillomavirus (HPV) types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in the general population in Karnataka, India. Asian Pac J Cancer Prev, 12, 645-8.
  17. Lim GCC, Rampai S, Yahaya H (eds) (2008). Cancer Incidence in Peninsular Malaysia (2003-2005). The Third Report of the National Cancer Registry of Malaysia. National Cancer Registry, Ministry of Health Malaysia, 1-179.
  18. Malaysian National Cancer Registry (MNCR) (2003). Cancer statistics. Ministry of Health Malaysia.
  19. Moore MA, Ariyaratne Y, Badar F, et al (2009). Cancer epidemiology in South Asia - past, present and future. Asian Pac J Cancer Prev, 11, 49-66.
  20. Munoz N, Bosch FX, de Sanjose S, et al (2003). Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med, 348, 518-27. https://doi.org/10.1056/NEJMoa021641
  21. Neville BW, Day TA (2002). Oral cancer and precancerous lesions. Cancer J Clin, 52, 195-215. https://doi.org/10.3322/canjclin.52.4.195
  22. Pindborg JJ, Reichart PA, Smith CJ, et al (1997). World Health Organization International Histological Classification of Tumours. Histological typing of cancer and precancer of the oral mucosa. 2nd ed. New York: Springer-Verlag, 33-40.
  23. Pintos J, Black MJ, Sadeghi N, et al (2006). Human papillomavirus infection and oral cancer. Oral Oncol, 44, 242-50.
  24. Ragin CC, Modugno F, Gollin SM (2007). The epidemiology and risk factors of head and neck cancer: a focus on human papillomavirus. J Dent Res, 86, 104-14. https://doi.org/10.1177/154405910708600202
  25. Reichart PA, Philipsen HP (2005). Oral erythroplakia - a review. Oral Oncol, 41, 551-61. https://doi.org/10.1016/j.oraloncology.2004.12.003
  26. Riley RR, Duensing S, Brake T, et al (2003). Dissection of human papillomavirus E6 and E7 function in transgenic mouse models of cervical carcinogenesis. Cancer Res, 63, 4862-71.
  27. Robert A (2000). Types of Oral Cancer in Oral Cancer, the dentist role in diagnosis, management, rehabilitation, and prevention (Robert AO, Remy HB, eds). Quintessence Publishing.
  28. SahebJamee M, Boorghani M, Ghaffari SR, et al (2009). Human papillomavirus in saliva of patients with oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal, 14, 525-8.
  29. Silverman S Jr (1998). Epidemiology. In: Oral Cancer (Silverman S Jr ed). pp1-6, 4th ed. Hamilton, Ontario, Canada: BC Decker Inc.
  30. Saini R, Tan PK, Sarah AR, et al (2010). High-risk human papillomavirus in the oral cavity of women with cervical cancer, and their children. Virology J, 7, 131-6. https://doi.org/10.1186/1743-422X-7-131
  31. Spielmann N, Wong DT (2011). Saliva: diagnostics and therapeutic perspectives. Oral Diseases, 17, 345-54. https://doi.org/10.1111/j.1601-0825.2010.01773.x
  32. Tan BS, Rosman A, Ng KH, et al (2000). Profile of the betel/tobacco quid chewers in six Malaysian estates. Annal Dent Univ Malaya, 7, 1-5.
  33. Zain RB (2001). Cultural and dietary risk factors of oral cancer and precancer - a brief overview. Oral oncol, 37, 205-10. https://doi.org/10.1016/S1368-8375(00)00133-0
  34. Zhao M, Jiang W, Carvalho AL, et al (2004). Feasibility of quantitative PCR-based saliva screening of HPV for head and neck cancer. Otolaryngology - Head and Neck Surgery, 131, 180. https://doi.org/10.1016/j.otohns.2003.05.001
  35. Zoltan S, Karoly P, L'aszlo T, et al (2005). Human papillomavirus in head and neck cancer: Molecular biology and clinicopathological correlations. Cancer Metast Rev, 24, 19-34. https://doi.org/10.1007/s10555-005-5045-3
  36. Zygogianni AG, Kyrgias G, Karakitsos P, et al (2011). Oral squamous cell cancer: early detection and the role of alcohol and smoking. Head and Neck Oncol, 3, 1-12. https://doi.org/10.1186/1758-3284-3-1

Cited by

  1. Association between the TP53 codon 72 polymorphism and risk of oral squamous cell carcinoma in Asians: a meta-analysis vol.14, pp.1, 2014, https://doi.org/10.1186/1471-2407-14-469
  2. Lack of Association between High-risk Human Papillomaviruses and Oral Squamous Cell Carcinoma in Young Japanese Patients vol.15, pp.10, 2014, https://doi.org/10.7314/APJCP.2014.15.10.4135
  3. Prevalence of Human Papillomavirus Infection in Oral Squamous Cell Carcinoma: a Case-control Study in Wuhan, China vol.15, pp.14, 2014, https://doi.org/10.7314/APJCP.2014.15.14.5861
  4. Emerging and Established Global Life-Style Risk Factors for Cancer of the Upper Aero-Digestive Tract vol.15, pp.15, 2014, https://doi.org/10.7314/APJCP.2014.15.15.5983