• Journal of Nutrition and Health
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• v.57 no.2
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• pp.185-195
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• 2024

Purpose: The carbohydrate-restricted diet has been recognized to be effective into preventing and alleviating lifestyle-related diseases, such as obesity and type 2 diabetes. The rare sugar ᴅ-allulose is a functional monosaccharide with anti-obesity effects. In the present study, we examined the effects of dietary ᴅ-allulose on body fat accumulation in rats fed severely carbohydrate-restricted diets containing high concentrations of different fats, beef tallow, or soybean oil. Methods: Male Wistar rats (n = 35, 3-week-old) were divided into 5 groups: One chow-fed control (C) group, and four carbohydrate-restricted groups, namely, beef tallow (B), beef tallow + ᴅ-allulose (BA), soybean oil (S), and soybean oil + ᴅ-allulose (SA), with free access to the diet and water for 8 weeks. The B and BA diets contained 23% beef tallow and 2% soybean oil, whereas the S and SA diets contained 25% soybean oil. Furthermore, the BA and SA diets contained 5% ᴅ-allulose. Results: The final body weight, weight gain, and food intake were significantly higher, and food efficiency was significantly lower in the control group compared to the other carbohydrate-restricted groups. Intra-abdominal adipose tissue, carcass fat, and total body fat weights were not influenced by dietary fat type or ᴅ-allulose supplementation, except for the epididymal adipose tissue weight. In contrast, carbohydrate restriction suppressed body weight gain in rats, but remarkably increased body fat accumulation. Conclusion: Under carbohydrate-restricted conditions, no anti-obesity effects of dietary ᴅ-allulose were observed, regardless of the dietary fat type. The causes of these effects are unknown. However, they may be influenced by a very low carbohydrate and high protein diet. Further research is required to elucidate the effects of ᴅ-allulose under various nutrient compositions with different fat, carbohydrate, and protein energy ratios.

• Archives of Plastic Surgery
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• v.39 no.1
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• pp.51-54
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• 2012

With the gradual increase of cases using fillers, cases of patients treated by non-medical professionals or inexperienced physicians resulting in complications are also increasing. We herein report 2 patients who experienced acute complications after receiving filler injections and were successfully treated with adipose-derived stem cell (ADSCs) therapy. Case 1 was a 23-year-old female patient who received a filler (Restylane) injection in her forehead, glabella, and nose by a non-medical professional. The day after her injection, inflammation was observed with a $3{\times}3cm$ skin necrosis. Case 2 was a 30-year-old woman who received a filler injection of hyaluronic acid gel (Juvederm) on her nasal dorsum and tip at a private clinic. She developed erythema and swelling in the filler-injected area A solution containing ADSCs harvested from each patient's abdominal subcutaneous tissue was injected into the lesion at the subcutaneous and dermis levels. The wounds healed without additional treatment. With continuous follow-up, both patients experienced only fine linear scars 6 months postoperatively. By using adipose-derived stem cells, we successfully treated the acute complications of skin necrosis after the filler injection, resulting in much less scarring, and more satisfactory results were achieved not only in wound healing, but also in esthetics.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.4
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• pp.590-595
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• 2008

The aim of our study was to research the effect of overfeeding on plasma parameters and mRNA expression of genes associated with hepatic lipogenesis in the Sichuan white goose and Landes goose. Fifty-four male Landes geese and 57 male Sichuan white geese were hatched on the same day under the same feeding conditions. After overfeeding for 14 days, (1) extrahepatic adipose tissues grew greatly in the Sichuan white geese, while more lipid accumulated in liver tissue in the Landes geese. (2) Sichuan white geese had a higher plasma concentration of triacylglycerols (TG), lipoproteins and insulin than the Landes geese. However, the Landes geese exhibited higher increase of plasma concentrations of TG, lipoproteins and insulin, with greater decrease of the diacylglycerol acyltransferase 2 (DGAT2) activity and DGAT2 mRNA level and a smller decrease of plasma glucose concentration. In addition, the mRNA level of MTP and LPL in liver was down- and up- regulated by overfeeding, respectively. (3) The correlations between the activity of LPL and the proportions of subcutaneous adipose tissue, abdominal adipose tissue, and liver weight, and the plasma concentration of VLDL were different in the two breeds. (4) The proportion of fatty liver weight was positively correlated to plasma concentrations of VLDL and TG in the overfed Sichuan white geese. Such a relationship did not exist in the Landes geese. (5) The activity of DGAT2 and its mRNA abundance in liver had significant negative correlations with the TG content in liver lipid and plasma insulin level in the Landes geese, while in the Sichuan white geese they had negative correlation (p>0.05) with TG concentration in liver lipid and had significant positive correlation with VLDL and TG concentrations in plasma.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.95-106
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• 2013

Objectives : The researcher investigated the anti-obesity effect of Alismatis Orientale Rhizoma(AP) water extract in mice fed a high fat diet and focused on the analysis of local area adipose tissue. Methods : Male ICR mice were divided into three groups, which were fed either a normal AIN diet, a 45% high fat diet (CT group), or a high fat diet and orally administration with a concentrations of 100 mg/kg (AP100 group) and 300 mg/kg body weight (AP300 group) for eight weeks. Results : As compared with CT group, AP100 group showed significant reductions in absolute weight of liver. As compared with CT group, AP100 group and AP300 group showed significant reductions in weight gain and relative weight of total fat. AST, triglyceride, total-cholesterol levels from the AP 100 group and 300 group were significantly lower than those of the CT, and ALT, LDL-cholesterol levels from the AP 100 group was significantly lower than those of the CT. But serum HDL-cholesterol levels from the AP 100 group and 300 group were significantly higher than those of the CT. And serum adiponectin levels from the AP 100 group was significantly higher than those of the CT. In result of real time PCR, all mRNA expression(PEPCK, G6Pase, PGC-$1{\alpha}$ and $ERR{\gamma}$) of two experimental groups were significantly decreased compared to those of CT group. The treatment with AP on local abdominal area made a fat cell size lessen on the fat tissue in the abdominal cavity and subcutaneous area. Conclusions : These results suggest that AP has an anti-obesity effect and the effect is mediated by inhibition of fat gain.

• Journal of Korean Medicine Rehabilitation
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• v.26 no.1
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• pp.13-31
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• 2016

Objectives In this study, it was investigated whether Gami-Handayeolso-Tang (HDYST) medication has anti-obesity effects in high fat diet (HFD)-fed obese mice. Methods The experimental animals were divided into five groups-normal diet-fed (ND), high fat diet-fed control (HFD), HFD+HDYST 150, HFD+HDYST 300, and HFD+orlistat as a positive drug. The obese markers such as body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, lipid contents, leptin, adiponectin, and GOT/GPT were measured. Also, white adipose tissue, liver weight, abdominal fat mass, hepatic lipid contents, and mRNA expression of obese-associating genes were examined in obese mice. Results In high fat diet-fed mice, HDYST administration significantly decreased body weight, diet efficiency ratio, serum levels of total cholesterol, triglyceride, LDL-cholesterol, as well as leptin and GOT/GPT, compared to the HFD group in a dose-dependent manner. HDYST increased significantly the serum levels of HDL-cholesterol and adiponectin. It also reduced the accumulation of lipids, such as total lipid and triglycerides, in organs such as liver and abdominal adipose tissue. Moreover, HDYST administration significantly decreased the expression levels of fatty acid synthetic genes, such as sterol regulatory element-binding protein-1c (SREBP-1c), FAS and Stearoyl-Coenzyme A desaturase 1 (SCD-1), in the liver tissues, while it increased the messenger RAN (mRNA) levels of fatty acid catalytic genes, such as Peroxisome proliferator activated receptor alpha (PPAR-${\alpha}$), acyl-COA oxidase (ACO), and Carnitine palmitoyltransferase-1a (CPT-1a). Conclusions Based on the results above, HDYST reveals anti-obesity effects declining body fat accumulation through the regulation of fatty acid metabolism and leptin/adiponectin serum levels. It therefore suggests that HDYST can be clinically useful for the treatment of obesity.

• Exercise Science
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• v.21 no.1
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• pp.111-120
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• 2012

The purpose of the study was to assess the effects of different cardiorespiratory fitness response to exercise training on cardiovascular disease and adipocytokine release and gene expression in abdominal obesity women. forty eight middle-age women were divided to a exercise-responder (n=34) group (ERG) and a exercise-nonresponder (n=14) group (ENRG) based on cardiorespiratory fitness after exercise training (12weeks, 1200 kcal/week, moderate-vigorous intensity, walking and jogging). Cardiorespiratory fitness was measured using maximal oxygen uptake with metabolic gas analysis and body composition was determined by bioelectrical impedance. We measured lipid and glucose profiles, blood adipocytokines and adipocytokine genes expression in adipose tissue. Waist girth (p=0.040), and %body fat (p=0.031) were significantly decreased in ERG than ENRG. Triglyceride (p=0.023) and systolic blood pressure (0.046) were significantly decreased in ERG than ENRG. Blood leptin (p=0.022) was significantly decrease in ERG than ENRG but leptin gene expression was decreased both groups (p<0.001). These results show that exercise-responder group improved cardiovascular disease risk factors and adipocytokine more than exercise-nonresponder group after exercise training. Moreover, exercise-nonresponders group show that obese indices and blood leptin and leptin gene expression in adipose tissue were decreased despite the failure to improve fitness. Therefore, regular exercise training seems to give health benefits although the failure to improve fitness.

• Archives of Plastic Surgery
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• v.36 no.6
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• pp.679-684
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• 2009

Purpose: Human lipoaspirate cells are relatively easy to obtain in large quantities without cell culture. The aim of this in vitro pilot study was to determine the effect of cell therapy using uncultured lipoaspirate cells on cell proliferation and collagen synthesis of diabetic fibroblasts, which are the major contributing factors in wound healing. Methods: In order to get diabetic fibroblasts, dermis tissues were obtained from foot skin of diabetic patients who underwent debridements or toe amputations(n = 4). In order to isolate lipoaspirate cells, the same diabetic patients' abdominal adipose tissues were obtained by liposuction. The diabetic fibroblasts were co - cultured with or without autogenous lipoaspirate cells using porous culture plate insert. Initial numbers of the lipoaspirate cells and diabetic fibroblasts seeded were 15,000 cells/well, respectively. For cell proliferation assay, two treatment groups were included. In group I, diabetic fibroblasts were cultured with the insert having no cells, which serves as a control. In group II, the lipoaspirate cells were added in the culture plate insert. For collagen synthesis assay, one additional group(group III), in which diabetic fibroblasts were not seeded in the well and only lipoaspirate cells inside the insert were incubated without diabetic fibroblasts, was included for a reference. Results: One hundred to one hundred sixty thousand lipoaspirate cells were isolated per ml of aspirated adipose tissue. After 3 - day incubation, the mean cell numbers in group I and II were 17,294/well and 22,163/well. The mean collagen level in group I, II, and III were 29, 41, and 2 ng/ml, respectively. These results imply that both cell proliferation and collagen synthesis in the lipoaspirate cell treatment group were 28 and 44 percents higher than in the control group, respectively(p < 0.05). Conclusion: Uncultured lipoaspirate cell autografts may stimulate the wound healing activity of diabetic fibroblasts.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.4
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• pp.383-393
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• 1991

The present study was carried out to investigate the effect of cimaterol on growth performance, carcass quality and cellular functional activity of broilers as affected by the various protein and energy levels. In starter period (0-21 days) all chicks were fed the basal diet which contained approximately 23 % crude protein and 3200 kcal of metabolizable energy per kg of diet. The cimaterol was added during 22-49 days and during the period of 8th week the cimaterol was withdrawn. In finisher period (22-49 days), a $2{\times}2{\times}3$ factorial arrangement consisting of 2 levels of cimaterol (0 mg/kg, 0.25 mg/kg), 2 levels of protein (19%, 17%) and 3 levels of energy (3200, 2900, 2600 kcal/kg) was used. In the finisher period, the body weight gain and feed efficiency was improved by the supplementation of cimaterol. The high protein and high energy level with supplementation of cimaterol had showed the highest body weight gain and feed efficiency, without significant difference. The administration of cimaterol had no effects on percentage of abdominal fat content, giblet and neck. Eventhough the difference was not significant (p>0.05), carcass yield was improved slightly by the administration of cimaterol. The effect of cimaterol on carcass composition was clearly demonstrated that protein content of broilers was not increased (p>0.05) but fat content decreased significantly (p<0.05). The ultilization of nutrients in experimental diets was not significantly affected by feeding cimaterol compared to control group. The results of in vitro studies with liver and adipose tissue showed that cimaterol increased the lipolytic activities at 19% protein level whereas at 17% protein level this effect was variable. Lipogenic activities in liver and adipose tissue were not affected with the administration of cimaterol but the activities increased as energy decreased, particularly in liver tissue. In cell studies with acinar culture of liver tissues, cimaterol had no effect on protein synthetic activity but the parameter was increased at higher level of dietary protein and energy. Protein secretion in liver was increased by the supplementation of cimaterol. In addition, at high protein level the protein secretion was increased and has shown the highest values at medium energy level.

• Journal of Nutrition and Health
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• v.26 no.1
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• pp.47-55
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• 1993

To investigate the effects of exercise-training on serum lipids, fat distribution and several parameters of body fatness(percent body fat, skinfolds thickness, body circumference) were assessed in 24 healthy male subjects submitted to an 8-wk high intensive exercise-training. Blood sample was taken twice, per and post exercise-training, Exercise-training took place 5 days a week and daily energy intake and expenditure were observed. The results obtained are summarized as follows: 1) Through exercise-training body weight (changes : 1.1$\pm$1.1kg ; p=0.000) and percent body fat (changes : 2.4$\pm$1.3% ; p=0.000) decreased significantly . 2) Suprailiac was the most reduced site by exercise-training among eight site skinfolds. Central skinfolds were changed more by exercise-training than peripheral skinfolds with reduction of 1.7$\pm$1.7mm and 0.2$\pm$1.9mm. Central site circumferences were reduced more by exercise-training than peripheral site circumferences. 3) Total cholesterol, HDL-cholesterol and LDL-cholesterol were not significantly changed by exercise-training while only triglyceride decreased (changes : 20.7$\pm$44.8mg/dl ; p<0.05). Changes in body weight were grately related to changes in total cholesterol. Changes in deep abdominal adipose tissue were related to changes in triglyceride.

• The Korean Journal of Food And Nutrition
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• v.16 no.3
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• pp.192-196
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• 2003

The purpose of this study was to investigate the changes of body composition, serum lipids and several parameters of body fatness (percent body fat, waist-hip ratio) in abdominal women by exercise. For this study, 8-weeks intensive exercise(5km jogging/day, 50min/day) was continued by subjects and they limited only fat rich foods and controlled daily energy intake to 1,800kcal～2,100kcal per day. The subjects were 52 women and the distribution of ages was 36～54 years. The data were analyzed using SPSS/PC package program and the results were estimated by paired t-test, Pearson correlation. The results are summarized as follows : 1) After exercise-training for 8 weeks, percent body fat, body mass index, body weight, total cholesterol was decreased (p<0.05). 2) LDL cholesterol and triglyceride was decreased significantly(p=0.000). The changes in deep abdominal adipose tissue were related to changes in triglycerides. 3) After exercise training, the waist-hip ratio was significantly correlated to body weight and serum lipids. 4) According to the data of this study, Ⅰ recommended that obese women, especially, abdominal obese patients should exercise regularly and we should prolong many studies for obesity.