Browse > Article

Effect of lipoaspirate cell autograft on proliferation and collagen synthesis of diabetic fibroblasts in vitro  

Song, Sun Ho (Department of Plastic Surgery Korea University College of Medicine)
Han, Seung Kyu (Department of Plastic Surgery Korea University College of Medicine)
Chun, Kyung Wook (Department of Plastic Surgery Korea University College of Medicine)
Kim, Woo Kyung (Department of Plastic Surgery Korea University College of Medicine)
Publication Information
Archives of Plastic Surgery / v.36, no.6, 2009 , pp. 679-684 More about this Journal
Abstract
Purpose: Human lipoaspirate cells are relatively easy to obtain in large quantities without cell culture. The aim of this in vitro pilot study was to determine the effect of cell therapy using uncultured lipoaspirate cells on cell proliferation and collagen synthesis of diabetic fibroblasts, which are the major contributing factors in wound healing. Methods: In order to get diabetic fibroblasts, dermis tissues were obtained from foot skin of diabetic patients who underwent debridements or toe amputations(n = 4). In order to isolate lipoaspirate cells, the same diabetic patients' abdominal adipose tissues were obtained by liposuction. The diabetic fibroblasts were co - cultured with or without autogenous lipoaspirate cells using porous culture plate insert. Initial numbers of the lipoaspirate cells and diabetic fibroblasts seeded were 15,000 cells/well, respectively. For cell proliferation assay, two treatment groups were included. In group I, diabetic fibroblasts were cultured with the insert having no cells, which serves as a control. In group II, the lipoaspirate cells were added in the culture plate insert. For collagen synthesis assay, one additional group(group III), in which diabetic fibroblasts were not seeded in the well and only lipoaspirate cells inside the insert were incubated without diabetic fibroblasts, was included for a reference. Results: One hundred to one hundred sixty thousand lipoaspirate cells were isolated per ml of aspirated adipose tissue. After 3 - day incubation, the mean cell numbers in group I and II were 17,294/well and 22,163/well. The mean collagen level in group I, II, and III were 29, 41, and 2 ng/ml, respectively. These results imply that both cell proliferation and collagen synthesis in the lipoaspirate cell treatment group were 28 and 44 percents higher than in the control group, respectively(p < 0.05). Conclusion: Uncultured lipoaspirate cell autografts may stimulate the wound healing activity of diabetic fibroblasts.
Keywords
lipoaspirate cell; diabetic fibroblast;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Embil JM, Papp K, Sibbald G, Tousignant J, Smiell JM, Wong B, Lau CY: Recombinant human platelet-derived growth factor-BB(becaplermin) for healing chronic lower extremity diabetic ulcers: an open-label clinical evaluation of efficacy. Wound Repair Regen 8: 162, 2000   DOI   ScienceOn
2 Hong L, Peptan IA, Colpan A, DAW JL: Adipose tissue engineering by human adipose-derived stromal cells. Cells Tissues Organs 183: 133, 2006   DOI   ScienceOn
3 Kano M, Masuda Y, Tominaga T, Hori T, Kitaichi T, Yoshizumi M, Kitagawa T: Collagen synthesis and collagenase activity of cryopreserved heart valves. J Thorac Cardiovasc Surg 122: 706, 2001   DOI   ScienceOn
4 Veves A, Falanga V, Armstrong DG, Sabolinski ML: Graftskin, a human skin equivalent, is effective in the management of noninfected neuropathic diabetic foot ulcers: a prospective randomized multicenter clinical trial. Diabetes Care 24: 290, 2001   DOI   ScienceOn
5 Kuroyanagi Y, Yamada N, Yamashita R, Uchinuma E: Tissue-engineered product: allogenic cultured dermal substitute composed of spongy collagen with fibroblasts. Artif Organs 25: 180, 2001   DOI   ScienceOn
6 Han SK, Choi KJ Kim WK: Clinical application of fresh fibroblast allografts for the treatment of diabetic foot ulcers: a pilot study. Plast Reconstr Surg 114: 1783, 2004   DOI   ScienceOn
7 Suga H, Matsumoto D, Inoue K, Shigeura T, Eto H, Aoi N, Kato H, Abe H, Yoshimura K: Numerical measurement of viable and nonviable adipocytes and other cellular components in aspirated fat tissue. Plast Reconstr Surg 122: 103, 2008   DOI   ScienceOn
8 De Loecker W, Koptelov VA, Grischenko VI, De Loecker P: Effects of cell concentration on viability and metabolic activity during cryopreservation. Cryobiology 37: 103, 1998   DOI   ScienceOn
9 Ashjian PH, De Ugarte DA, Katz AJ, Hedrick MH: Lipoplasty: from body contouring to tissue engineering. Aesthet Surg J 22: 121, 2002   DOI   ScienceOn
10 Sams HH, Chen J, King LE: Graftskin treatment of difficult to heal diabetic foot ulcers: one center's experience. Dermatol Surg 28: 698, 2002   DOI   ScienceOn
11 Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279, 2002   DOI   ScienceOn
12 Marston WA, Hanft J, Norwood P, Pollak R: The efficacy and safety of dermagraft in improving the healing of chronic diabetic foot ulcers: result of a prospective randomized trial. Diabetes Care 26: 1701, 2003   DOI   ScienceOn
13 Hehenberger K, Heilborn JD, Brisma K, Hansson A: Inhibited proliferation of fibroblasts derived from chronic diabetic wounds and normal dermal fibroblasts treated with high glucose is associated with increased formation of l-lactate. Wound Repair Regen 6: 135, 1998   DOI   ScienceOn
14 Dragoo JL, Choi JY, Lieberman JR, Huang J, Zuk PA, Zhang J, Hedrick MH, Benhaim P: Bone induction by BMP-2 transduced stem cells derived from uman fat. J Orthop Res 21: 622, 2003   DOI   ScienceOn
15 Liu K, Yang Y, Mansbridge J: Comparison of the stress response to cryopreservation in monolayer and three- dimensional human fibroblast cultures: stress proteins, MAP kinases, and growth factor gene expression. Tissue Eng 6: 539, 2000   DOI   ScienceOn