• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.9
• /
• pp.1210-1214
• /
• 2009

Early postmortem ATP level is known as a good predictor of pork quality. Pork carcasses were divided into two; one was electrically stimulated (ES) to simulate poor quality pork and the other was left untreated and served as a control normal pork. Fractions of longissimus were excised from carcasses regularly for 2 hours after death and deep-frozen ($-80^{\circ}C$) until analyses. The ATP level of normal untreated control pork decreased from 5.00 to 2.04 ${\mu}mole$/g within 2 hours postmortem. The decrement of ATP was approximately 60% of its initial content. In the meantime, ES poor quality pork had a more drastic rate of ATP decrease. Electrical stimulation itself decreased ATP level from 4.70 to 3.50 ${\mu}mole$/g, by approximately 25%. ATP level of ES pork dropped to 1.71 ${\mu}mole$/g within 1 hour postmortem and was further plunged to 0.26 ${\mu}mole$/g and almost exhausted during the next hour. The level of IMP increased from 0.49 to 3.17 ${\mu}mole$/g and it became the dominant nucleotide within 2 hours postmortem. Electrical stimulation prompted the increase of IMP from 0.69 to 3.19 ${\mu}mole$/g and its level went up to 6.64 ${\mu}mole$/g within 2 hours postmortem. The level of ADP also decreased from 1.45 to 0.67 ${\mu}mole$/g for 2 hours after death and ES also accelerated ADP breakdown. The AMP levels were lower than those of other nucleotides and increased from 0.16 to 0.31 ${\mu}mole$/g within 2 hours postmortem. The increase of AMP was accelerated between 60 and 90 minutes after electrical stimulation. Early postmortem electrical stimulation prompted a drastic rate of changes in contents of 4 nucleotides during 2 hours postmortem. In the meantime, the ATP levels for ES poor quality pork were much lower than those of normal pork.

• The Journal of the Korean Society for Microbiology
• /
• v.35 no.2
• /
• pp.109-115
• /
• 2000

FtsH is a membrane-bound, ATP-dependent metalloprotease that is involved in a variety of cellular functions including the regulation of responses to heat and stress shock. Previously, we had cloned and sequenced pneumococcal ftsH gene whose deduced amino acid sequence was very similar to those of several gram-positive bacteria and Escherichia coli, except for the N-terminal domain that was responsible for membrane anchoring. In order to better understand the role of Streptococcus pneumoniae FtsH, we expressed pneumococcal ftsH gene in Escherichia coli. When it was expressed from a strong promoter, $P_{tac}$, a considerable amount of the recombinant FtsH was produced, although the prolonged induction resulted in not only accumulation of breakdown products but also ceasing of the further growth of E. coli host. This indicated that the expression of the exogenous ftsH gene was tightly regulated since the excessive FtsH appeared detrimental to bacterial cells. In Western blotting, the pneumococcal FtsH protein, whether native or recombinant, was reactive to anti-E. coli FtsH serum. The observation that FtsH proteins were well conserved throughout the bacterial kingdom and its expression level was fine-tuned suggests an important role for this protein in the stress adaptation which may be related to infecting process by pneumococci.

• The Korean Journal of Physiology and Pharmacology
• /
• v.22 no.3
• /
• pp.225-234
• /
• 2018

Adenosine is a naturally occurring breakdown product of adenosine triphosphate and plays an important role in different physiological and pathological conditions. Adenosine also serves as an important trigger in ischemic and remote preconditioning and its release may impart cardioprotection. Exogenous administration of adenosine in the form of adenosine preconditioning may also protect heart from ischemia-reperfusion injury. Endogenous release of adenosine during ischemic/remote preconditioning or exogenous adenosine during pharmacological preconditioning activates adenosine receptors to activate plethora of mechanisms, which either independently or in association with one another may confer cardioprotection during ischemia-reperfusion injury. These mechanisms include activation of $K_{ATP}$ channels, an increase in the levels of antioxidant enzymes, functional interaction with opioid receptors; increase in nitric oxide production; decrease in inflammation; activation of transient receptor potential vanilloid (TRPV) channels; activation of kinases such as protein kinase B (Akt), protein kinase C, tyrosine kinase, mitogen activated protein (MAP) kinases such as ERK 1/2, p38 MAP kinases and MAP kinase kinase (MEK 1) MMP. The present review discusses the role and mechanisms involved in adenosine preconditioning-induced cardioprotection.

• The Transactions of the Korean Institute of Electrical Engineers A
• /
• v.54 no.8
• /
• pp.410-417
• /
• 2005

This paper analyses the transient phenomena against single line to ground fault and lightning surge on underground power cable systems. For analysis in various fault conditions, several actual underground power cable systems are modeled using ATP In ground fault, the transient characteristic of the conductor and the sheath according to the fault current and the installation types of CCPU are analysed. In lightning surge strokes, the various unbalanced conditions including the length of crossbonded lead, the breakdown of CCPU and distance unbalance are considered. This paper is expected to contribute the establishment of proper protection methods against transients on underground power cable systems.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.60 no.10
• /
• pp.1811-1816
• /
• 2011

This paper describes switching surge analysis for reclosing decision in 345kV combined transmission line with GIL. Reclosing operation should be decided based on the detailed technical analysis in combined transmission line because this line includes power cable section which is week on insulation. Insulation of power cable can be breakdown at the week point in case of reclosing moment. Therefore the detailed analysis has to be carried out by considering several conditions such as charging rate, inserting resistance, arrester, length ratio of power cable section, grounding resistance, etc. Analysis is performed by EMTP/ATP.

• Proceedings of the KIEE Conference
• /
• 2005.07a
• /
• pp.718-720
• /
• 2005

This paper analyses the transient phenomena against lightning surge on underground power cable systems. For analysis, several actual underground power cable systems are modeled using ATP. In lightning surge strokes, the various unbalanced conditions including the length of crossbonded lead, the breakdown of CCPU and distance unbalance are considered. This paper is expected to contribute the establishment of proper protection methods against transients on underground power cable systems.

• The Transactions of the Korean Institute of Electrical Engineers A
• /
• v.54 no.10
• /
• pp.487-495
• /
• 2005

This paper reviews the characteristic of thermal and temperature of oil field cable in transient state such as grounding fault and lightning surge. For analysis in various conditions, many actual underground power cable systems are modeled using ATP. These results are applied for the examination of temperature increase when the single line to ground fault by the breakdown of insulation part and hitting of lightning surge are occurred. The inner part temperature of OF cable is analysed according to the various kinds of cable using the thermal model of transient state. The temperature increase of sheath and crossbonded lead bv fault current is also analysed using IEC 60949.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.27 no.5
• /
• pp.501-508
• /
• 1994

This study was undertaken to get basic data for the cold-waterless transportation of live fish. The optimal cold temperature of plaice, Paralichthy olivaceus was determined by checking the changes of dissolved oxygen and ammonia in the sea water and the survival times during storage at various temperatures. After determination of optimal temperature for transportation, the changes of serum components and muscle components of live plaice were also carried out during storage at cold($5^{\circ}C$)-waterless conditions and the recovery conditions($10\%$ density at $15^{\circ}C$). At higher storage temperature, decreases in dissolved oxygen and the increases in ammonia in seawater were observed. In addition, the survival time was short at low temperature($0^{\circ}C\;and\;3^{\circ}C$). Almost all of the serum components(hemoglobin, glucose, LDH, GOT and GPT) of live plaice gradually increased during storage in cold-waterless conditions, and then those values decreased to the initial levels after $3{\sim}10hrs$ storage in conditions of recovery. The concentration of ATP in the muscle steadily decreased during storage in cold-waterless conditions. The contents of ADP and IMP seemed to be directly related to the extent of ATP breakdown. ADP and IMP thus showed a gradual increase during storage. The level of lactate in the muscles gradually increased during these storage times, also. On the other hand, the levels of those components in the muscle entirely recovered to their original levels within $3{\sim}6hrs$ storage after they were returned to conditions of recovery. The ratio of ATP to the ATP and its related compounds${ATP/(ATP+ADP+AMP+IMP){\times}100}$ in the muscle showed $45\%$ after 18hrs storage in cold-waterless conditions. Otherwise, ratios returned to their original levels within $3{\sim}6hrs$ of storage in recovery conditions.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.8
• /
• pp.1336-1344
• /
• 2018

Objective: This study aimed to observe whether high-pressure processing (HPP) affected aroma development and the degradation rate of umami taste-related ATP breakdown products, specifically inosinic acid in grass-fed beef during vacuum aging. Methods: Strip loin (longissimus lumborum) cuts obtained from six grass-fed Friesian Holstein steers (32 months old) on day 4 post slaughter were vacuum-packed and subjected to pressurization at 300 and 500 MPa for 180 s at $15^{\circ}C{\pm}2^{\circ}C$. The samples were then stored for 4 weeks at $5^{\circ}C{\pm}0.5^{\circ}C$ under vacuum and compared with the control (0.1 MPa). Results: HPP increased the shear force value, promoted moisture loss and lipid oxidation, induced surface paleness, stabilized pH during aging, and reduced bacterial load and growth. The shear force value of 500 MPa-treated samples remained higher than the control after aging, while no significant differences were found between the control and 300 MPa-treated samples. Degradation of inosinic acid and inosine occurred during pressurization, resulting in an increase in hypoxanthine content. However, the degradation rate in HPP-treated samples during aging was slower; therefore, inosinic acid and inosine content remained higher than in control samples. No significant differences were found in hypoxanthine content at the end of aging. HPP intensified the levels of hexanal, octanal, 2-methylbutanal, 3-methylbutanal, benzaldehyde, and 2,5-dimethylpyrazine in cooked-aged beef samples. Conclusion: HPP induced aroma development and delayed the degradation of inosinic acid. However, it also reduced the postmortem tenderization rate.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.59 no.5
• /
• pp.847-853
• /
• 2010

This paper describes switching surge analysis for reclosing decision in 345kV combined transmission line with XLPE power cable. Reclosing operation should be decided based on the detailed technical analysis in combined transmission line because this line includes power cable section which is week on insulation. Insulation of power cable can be breakdown at the week point in case of reclosing moment. Therefore the detailed analysis has to be carried out by considering several conditions such as length ratio of power cable section, arrestor, inserting resistance, charging rate, grounding resistance, etc.. On the other hand sheath voltage on IJ(Insulated Joint) is analyzed to check dangerous condition on cable cover. Analysis is performed by EMTP/ATP. Analysis results show that reclosing can be operated as the single line-to-ground fault occurs on overhead line in 345kV combined transmission line, if the inserting resistance is considered before the operation of main circuit breaker.