• The Journal of Korean Academic Society of Nursing Education
• /
• v.20 no.2
• /
• pp.312-320
• /
• 2014

Purpose: There recurred controversies related to legal allowance of abortion since newly advancing prenatal genetic testing technology. This survey was aimed to identify the attitudes toward marriage, and pregnancy (AMP) and terminating pregnancy (ATP) among Korean nursing students. Method: A descriptive study was performed from October to November 2012 in South Korea. 424 nursing students responded to two types of tools developed by authors. Questionnaire for ATP is consisted of 10 items with 5-points Likert Scale. Questionnaire for AMP is 12 items containing 5 categories. Results: Mean score of ATP was 36.90. Students that are enrolled in bachelor degree had a significant higher ATP. Students who are currently dating with an opposite gender showed much higher level of ATP than those are not. Lower group of delayed the time of getting marriage and pregnancy showed highest score of ATP, significantly. Conclusion: Individual factor must be primarily considered when assessing ATP of nursing students, for example current relationship with opposite gender and educational background, attitude toward marriage and pregnancy. Further studies are needed to identify personal factors of the young adults affecting on the ATP.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1997.04a
• /
• pp.97-97
• /
• 1997

It was reported that ATP depletion occurs and accelerates cell damage during ischemia and reperfusion. To determine the mechanism of cell damage, the change of energy metabolism in liver was studied during ischemia/reperfusion. The groups were divided into four categories : sham-operated group, ischemia/reperfusion group, and two types of ATP-MgCl$_2$ treatment groups(one was treated during ischemia and the another during reperfusion). Rats were administered intravenously saline or ATP-MgCl$_2$. Rats were anesthetized and blood vessels in the left and median lobes of the liver were occluded. After 60min of ischemia, the clamp at those vessels were removed. After ischemia, one and five hours after reflow, energy metabolites(ATP, ADP, AMP, inosine, adenosine, hypoxanthine, xanthine) in liver were measured with HPLC. To observe mitochondrial function, aterial keton body ratio in blood and mitochondrial glutamate dehydrogenase activity in liver were measured. And lipid peroxidation was measured to evalutate the involvement of free radicals. In this study, ATP and ADP were catabolized to their metabolites(AMP, inosine, adenosine, hypoxanthine, xanthine) during ischemia and they resynthesized ATP and ADP during reperfusion. But total purine base were not restored to level of normal rat. The main source of resynthesizing ATP and ADP was AMP. In both ATP-MgCl$_2$ treated groups, mitochondrial function was protected and lipid peroxidation was significantly reduced. Our findings suggest that ischemia/reperfusion impairs hepatic energy metabolism.

• Health Policy and Management
• /
• v.14 no.4
• /
• pp.75-87
• /
• 2004

The purpose of this paper is to compare the amount of household health expenditures (HHE) and the proportion of health care expenditure to the household expenditure by ability to pay(ATP) levels. This study has focused on the influence of household ATP on HHE, estimating elasticity of health care expenditures for different ATP groups. For the empirical analysis, the Urban Households Survey Data of 2002 have been used. Our principal findings show that HHE are sensitive to changes in household ATP levels and that the group which is most responsive to changes in A TP level is the lower ATP group. These suggest that as households have less ATP, households with lower ATP reduce expenditures on health care in a proportional manner than those with higher ATP.

• YAKHAK HOEJI
• /
• v.45 no.3
• /
• pp.282-286
• /
• 2001

To investigate the different mechanism between ATP and compound 48/80 (C$_{48}$80/)-induced histamine release, we observed effects of calcium antagonists in histamine release of rat peritoneal mast cells. Verapamil and diltiazem (voltage-dependent calcium channel blocker) and TMB-8 (a blocker of intracellular calcium release) significantly inhibited ATP-induced histamine release, but did not inhibit $C_{48}$80/-induced histamine release. Econazole (a blocker of receptor-operated calcium channel) dose-dependently inhibited both ATP and $C_{48}$80/-induced histamine release, but inhibitory effect of econazole in ATP-induced histamine release was more potent than that in $C_{48}$80/-induced histamine. EGTA dose-dependently inhibited ATP and $C_{48}$80/-induced histamine release, but $C_{48}$80/-induced histamine release was slightly inhibited by high concentrations (>2 mM) of EGTA. These results suggest that ATP-induced histamine release is related to broth intracellular calcium release and extracellular calcium influx via voltage-dependent calcium channel and receptor-operated calcium channel. $C_{48}$80/-induced histamine release is related to extracellular calcium influx, especially by receptor-operated calcium channel rather than voltage-dependent calcium channel.

• Proceedings of the KSR Conference
• /
• 2009.05a
• /
• pp.1959-1962
• /
• 2009

In this paper, we derive the hazard which is a criterion of safety assurance by PHA for the ATP equipped in Korea Tilting Train. The ATP onboard equipment in Korea Tilting Train was introduced as ETCS Level l(which is ETCS specification). Also, the ATP trackside equipment was introduced as a ETCS SRS(System Requirement Specification) 2.2.2 and it has a target for commercial operation in 2009 late. In IEC 62278(EN50126), it recommend of PHA to derive a hazard of target system in concept establishment step and estimate if it can control as a tolerable level and then derive safety case to control the hazard by lifecycle. Therefore, we indicate the hazard using criterion of safety assurance by PHA using FRS (Functional Requirement Specification) 5.0. which is a upper criterion than SRS. The hazard of ATP onboard equipment derived in Korea Tilting Train will be able to use as ATP functional hazards of unified onboard equipment in G7 which is a multiple unit and preliminary hazards of ATP onboard equipment in NEL drived by Korail.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.2
• /
• pp.224-230
• /
• 2006

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. We report here the kinetic mechanism of presteady state ATP binding and hydrolysis by the Rho-RNA complex. Presteady state chemical quenched-flow technique under multiple turnover condition was used to probe the kinetics of ATP binding and hydrolysis by the Rho-RNA complex. The quenched-flow presteady state kinetics of ATP hydrolysis studies show that three ATPs are bound to the Rho-RNA complex with a rate of $4.4\;{\times}\;10^5M^{-1}s^{-1}$, which are subsequently hydrolyzed at a rate of $88s^{-1}$ and released during the initiation process. Global fit of the presteady state ATP hydrolysis kinetic data suggests that a rapid-equilibrium binding of ATP to Rho-RNA complex occurs prior to the first turnover and the chemistry step is not reversible. The initial burst of three ATPs hydrolysis was proposed to be involved in the initialization step that accompanies proper complex formation of Rho-RNA. Based on these results a kinetic model for initiation process for Rho-RNA complex was proposed relating the mechanism of ATP binding and hydrolysis by Rho to the structural transitions of Rho-RNA complex to reach the steady state phase, which is implicated during translocation along the RNA.

• The Korean Journal of Mycology
• /
• v.17 no.4
• /
• pp.177-183
• /
• 1989

Mitochondria in Pleurotus ostreatus was purified by stepped sucrose density gradient centrifugation. The mitochondrial ATP synthase was investigated by various waveof the illumination at dark room for 30 min. The mitochondrial ATP synthase activity was stimulated 2.3 fold by 480 nm illumination compared with the broad wavelength group. The mitochondrial ATP synthase activity according to various times of illumination was stimulated 4.2 fold for 15 min at 480 nm compared with the broad wavelength group. The optimum pH and optimum temperature of the mitochondrial ATP synthase were 7.5 and $56^{\circ}C$, respectively. The activity of this enzyme was stimulated by 0.5 mmol $Fe^{2+}$, 1.0 mmol $Fe^{3+}$ and 5.0 mmol $k^+$ ion, but inhibited by 0.1 mmol $Na^+$ ion.

• Industrial Engineering and Management Systems
• /
• v.7 no.1
• /
• pp.57-65
• /
• 2008

Available-to-promise (ATP) exhibiting availability of manufacturing resources can be used to support customer order promising. Recently, one advanced function called Capable-to-promise (CTP) is provided by several modern APS (advanced planning system) that checks available capacity for placing new production orders or increasing already scheduled production orders. At the customer enquiry stage while considering the order delivery date and quantity to quote, both ATP and CTP are allocated to support order promising. In particular, current trends of mass customization and multi-side production chain derive several new constraints that should be considered when ATP/CTP allocation planning for order promising - such as customer's preference plants or material vendors, material compatibility, etc. Moreover, ATP/CTP allocation planning would be executed over a rolling time horizon. To utilize capacity and material manufacturing resource flexibly and fulfill more customer orders, ATP/CTP rolling planning should possess resource reallocation mechanism under the constraints of order quantities and delivery dates for all previous order promising. Therefore, to enhance order promising with reliability and flexibility to reallocate manufacturing resource, the ATP/CTP reallocation planning mechanism is needed in order to reallocate material and capacity resource for fulfilling all previous promised and new customer orders beneficially with considering new derived material and capacity constraints.

• Molecules and Cells
• /
• v.38 no.7
• /
• pp.616-623
• /
• 2015

P2 receptors are membrane-bound receptors for extracellular nucleotides such as ATP and UTP. P2 receptors have been classified as ligand-gated ion channels or P2X receptors and G protein-coupled P2Y receptors. Recently, purinergic signaling has begun to attract attention as a potential therapeutic target for a variety of diseases especially associated with gastroenterology. This study determined the ATP and UTP-induced receptor signaling mechanism in feline esophageal contraction. Contraction of dispersed feline esophageal smooth muscle cells was measured by scanning micrometry. Phosphorylation of $MLC_{20}$ was determined by western blot analysis. ATP and UTP elicited maximum esophageal contraction at 30 s and $10{\mu}M$ concentration. Contraction of dispersed cells treated with $10{\mu}M$ ATP was inhibited by nifedipine. However, contraction induced by $0.1{\mu}M$ ATP, $0.1{\mu}M$ UTP and $10{\mu}M$ UTP was decreased by U73122, chelerythrine, ML-9, PTX and $GDP{\beta}S$. Contraction induced by $0.1{\mu}M$ ATP and UTP was inhibited by $G{\alpha}i_3$ or $G{\alpha}q$ antibodies and by $PLC{\beta}_1$ or $PLC{\beta}_3$ antibodies. Phosphorylated $MLC_{20}$ was increased by ATP and UTP treatment. In conclusion, esophageal contraction induced by ATP and UTP was preferentially mediated by P2Y receptors coupled to $G{\alpha}i_3$ and $G{\alpha}q$ proteins, which activate $PLC{\beta}_1$ and $PLC{\beta}_3$. Subsequently, increased intracellular $Ca^{2+}$ and activated PKC triggered stimulation of MLC kinase and inhibition of MLC phosphatase. Finally, increased $pMLC_{20}$ generated esophageal contraction.

• Childhood Kidney Diseases
• /
• v.16 no.2
• /
• pp.109-114
• /
• 2012

Purpose: Medullary sponge kidney (MSK) is a rare congenital disease characterized by diffuse ectasia or dilatation of precalyceal collecting tubules. MSK incidence and prevalence in the general population is uncertain and only a few patients are reported especially in the pediatric age. There has been increasing reports of patients with MSK who have other malformative disorders. Also several case reports concerning about etiological association of some genes. Methods: Collaborative study through nation-wide survey was done to investigate the incidence and etiological association of some genes such as GDNF gene, ATP6V1B1, ATP6V0A4 gene in developing MSK in Korean children. Results: Four cases of MSK who have various other malformative disorders were collected. There are no mutations of GDNF gene, ATP6V1B1, ATP6V0A4 gene in all patients. Conclusion: MSK is one of the very rare diseases in pediatric age. The etiological association of GDNF gene, ATP6V1B1, ATP6V0A4 gene in developing MSK in Korean children is not proved.